Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 458,189 | A→G | 15.9% | D255G (GAT→GGT) | clpX → | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 458,189 | 0 | A | G | 15.9% | 173.3 / 4.6 | 68 | D255G (GAT→GGT) | clpX | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
Reads supporting (aligned to +/- strand): ref base A (5/49); new base G (9/2); total (17/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.91e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA > NC_000913/457952‑458425 | cacaGGCTGGTATTGTCTTCATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGAt < 1:994742/239‑1 (MQ=255) gggtttttttGTCTACATCGATGAAACCGACCAGATTTTTG‑GCAAGCCAGAACACCCGTCCATGACCCGAGGCGTTGCCGGTGAAGGCGTACGGCCGGCCATGGTGGAGCGGATCGAAGGTACGGTAGCTGCTGTTCCACAGCAAGGTGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTCATAACTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAg < 2:995581/231‑1 (MQ=255) agagacagttGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAg < 1:606801/231‑1 (MQ=255) gTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAg < 1:189820/237‑1 (MQ=255) gtctcaaTCGATGAGATCGACATAGTTTCGC‑GAAAGTCAGACAACCCGTCCATTTCCCTGGGCGTTTCCGGTGAAGGCGTCCCGCAGGCACTGTTGAAACTGAGCGAAGGTACGGTCGCTGCTGTTCCACCGCAAGGGGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCa < 2:1289911/233‑1 (MQ=255) gTCTAAATCGATGAAAGCGACAAGGTTTCGC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGGTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCa < 1:786145/239‑1 (MQ=255) cTACATCGATGAAAGCGACAAGA‑TTCTCGGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAc < 1:1244916/238‑1 (MQ=255) cTAAATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAcc < 1:1123031/239‑1 (MQ=255) tACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTTGCGGTGCGGTTTCCGGTCTGGTTAAAAGGATTTTTCAccc > 1:1235085/1‑238 (MQ=255) aTCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGCTTTTCTGTCTGTCTAAAGTGAGTTTCCAcccgct > 1:970279/1‑235 (MQ=255) tGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCTGGGCTTGGTAAAATGATTTcccctgtctctttt > 1:1015161/1‑227 (MQ=255) tGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGAGATTTCCAACCctgtgggaa > 1:1139855/1‑234 (MQ=255) aGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGGTAAAAGGATTTTCCCCCGTGGAGAAAACGGCCCCGGCC‑TTTGTTTTggg > 1:537281/1‑238 (MQ=255) caacacaCCCGTCCATTACCCGAGGCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGgcgc < 1:1002558/232‑1 (MQ=255) gACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCGGGTTAAATGGATTTCTCCCCGGGTGGCAAACGCACCCGGCC‑TTGGgttttcacagcg > 1:80187/1‑231 (MQ=255) cAACCCGTCCATTGCCCGATCCGGTTCCGGTGAAGGCGTACCGCAGGCACGGTTGAACCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTATGGATAAAGTGATTTCCCTCCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACgg < 1:714740/239‑1 (MQ=255) cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATaaaa > 1:517912/1‑178 (MQ=255) cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAg < 2:517912/179‑1 (MQ=255) aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGaa < 1:949766/239‑1 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTTCCGGGCTTGGTAAAGTGGTTTTCCCCCCTGGAGGAACCGGCCCCCGCA‑TTGGGTTTGGTGCGACGGTTAAACGCAAGTcccgca > 1:781949/1‑235 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTTCGGTCTGGGTAAAAGGATTTCCCCCCGTGTAGAAACCCGCTCCGGCC‑TTGGTTTTGGCGCGACGGTCAAAGCGAAGTCCGACa > 1:203872/1‑239 (MQ=255) cTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTCTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt < 1:996759/239‑1 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTTCGTTTGCCGGACTGGGTAAAGTGCATTTCCACCGTGTAGAAACCGGCTCCGGCATTTGGTTTTGG‑GGGCCGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGGCGCTGGCGCCGGTTg > 1:201529/1‑239 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGTCTTGATAAAGGGATTTTCCACCGTGTAGAAAACGGCTCCGGCA‑TTGGTTTTTGCGCGGCGGGAAAAGAGAAGGCCGGCAAAACAAACGAAGGCGAGCTGCTGGGCCAGGGTg > 1:686838/1‑239 (MQ=255) gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGCGGGCTGGATAAAATGATTTTCCCCCGCGTAGAAAACAGGCCCGGCA‑TTTGGTTTGGGGCGGCGGTGAAAGCGAAGAGCGCCAAAAAAAGCGAAGGGGGGCTGCTggggggggggg > 2:1196546/1‑230 (MQ=255) gTTGAAACTGAACGAAGGTAGGTGAGTGGCTGTTCCACCGTAAGGTGGGCGTAAACAACCCGAGAAGGAATTCTTGGAGGTCGATACCTGTAAGATCCTGTTTATTTGGGGCGGTGCGGTTGCCGGTCTGGATAAAGTGAGTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCTCAGGTTg < 2:201529/239‑1 (MQ=255) ttGAAACTGATCGAGGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAGCATCCGCAGCAGGAATGCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa < 1:197806/239‑1 (MQ=255) ttGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCTGGGTAAAATGATTTTCCACCGTGTCGAAAACCGCCCCGGCA‑TTGGGTTTGGTGCGCCGGGAAAAGAGAAGACCGGCAAAGCAAGCGAAGGCGGCCTGCTGTGGCCGGTTGa > 1:1074885/1‑239 (MQ=255) ttGAAACTGACTGAAGGTGCGTGGTCTGCTGTGCCACCGCACGGGTGGCGTTAACATCCGCATCAGGAATTCGTGAGGGTTGATACCGTTAAGCTCCTGTTGATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGGGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa < 2:781949/239‑1 (MQ=255) ccTGATCGAAGGGACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg < 1:170455/238‑1 (MQ=255) tCGAAGGTACGGTAGCTGCGGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt < 1:537719/239‑1 (MQ=255) cGAAGGTTCGGTAGGTGCTGTCCCCCCGCAAGGTTGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt < 1:511365/238‑1 (MQ=255) tACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg < 1:922143/214‑1 (MQ=255) gtcgctcttgTTCCCCCGCAATTGTGGCGTAAAATTCCGTAGCAGGAATTCTTGACGGTTGATACCTCTAAGGTCCTGTTGTTTTGTGGCGGTGCGTTTGCCGGTCGGGATAAAGTGATTTCCCACCGGTTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGGGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 2:644447/231‑1 (MQ=255) tAGCTGCTGTTCCACCGGAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:1094244/238‑1 (MQ=255) tAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACCTCCGCAGCAGGAATTCTTGCAGGTTTATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:1205243/238‑1 (MQ=255) ggctgcgGTTCCCCCTGAAGGGGGGGGTAAACATCCGAAGCAGGAATTCTGTCAGGTTGATACCTCGAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt < 2:834255/237‑1 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTg > 1:32436/1‑111 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTg < 2:32436/111‑1 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt < 1:1196546/239‑1 (MQ=255) ccACCGGAATGTGGGCTGAACCATCCGCAGCAGGAAATCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 1:870587/239‑1 (MQ=255) ccACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 1:568557/239‑1 (MQ=255) cccctgaaGGTGGGTATAAACCTCCGGAGCAGTAATTCTTGCAGTTTCGTCCCTCTAAGAGCCTGTTTGTTTGTGGCGGCGCGTTTGGCGGGCCGGATAAAGTGAGTGCCCACCGTGTAGAAACCGGCTTCTGCC‑TTTGTTTGGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGACGAGCTGCTGGCGCAGGTTGAACCGGAATATCTGATCAAGTTTGGTCTTATCCCt < 2:313096/233‑1 (MQ=255) ccccGTAAGGTGGGCTTAACCATCCGGAGCAGGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTCTATTTGTGGCGGTGCGGTGTCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt < 2:260982/237‑1 (MQ=255) gccGCAAGCGGGGCGTAAACATCCGGAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCAGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg < 1:177259/238‑1 (MQ=255) ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:539900/224‑1 (MQ=255) gggAATTTTTGCAGGTTGATACCTCTAAGATCATGTTTAATTGGTGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa < 1:958429/238‑1 (MQ=255) agGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCg < 1:382926/237‑1 (MQ=255) aaTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATATAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc < 1:983329/238‑1 (MQ=255) aaTTCTTGAAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCCCCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGATAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCa < 1:673463/236‑1 (MQ=255) ttGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg < 1:1208849/159‑1 (MQ=255) ttGATACCTCTAAGGTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa < 1:823613/236‑1 (MQ=255) ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc < 1:168042/179‑1 (MQ=255) ctctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaag < 1:857284/239‑1 (MQ=255) tctAAGGTCCTGTTTATTTGGGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑gaaga < 1:974074/239‑1 (MQ=255) aTCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGa < 1:942758/38‑1 (MQ=255) aTCATGTTTATTGGGTGCGGTGCGTTTGCCGGTCTGGATAAAGGTATTACCGACCGTGTAGAAACCGGCTACGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGctct < 1:435958/239‑1 (MQ=255) cTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGGTAAAGTGATTTTCCACCGTGTAGAAAACGGCTCCGGCA‑TTGGGTTTGGCGCGACGGGAAAAACGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGGCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTGTTGGCAACGTTGAATTAACTGAGC‑GAGGAAGCTCTGAt > 1:326541/1‑239 (MQ=255) tGTTTATTTGGGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGAtt < 1:1105375/239‑1 (MQ=255) tttATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa < 1:910734/239‑1 (MQ=255) tttATTTGGGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCa < 1:1156287/239‑1 (MQ=255) tgGCGGTGCGTTTGCCGGTCTGGATAAAGTGCTTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGGCGGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATATGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa < 2:203872/239‑1 (MQ=255) ggggggTGCGTTTGGCGGTCGGGAGAAAGGGAGTTCCCACGGTGTAGAAACCGGCTCAGGCA‑TTGGGTTTGGCCCGGGCGTAAAAGCGAAGTCCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTTTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCa < 2:1074885/235‑1 (MQ=255) ggggTGCTTTTTCTGGGCTGAGTAAAGGTATTTCACACCGTGTAGAATCCGGCTTGGGCA‑TTGGTGTCGGCGCGACGGTAAAAGCGAAGTCCGTCAAAGCAAGCGAAGGTGAGATGCTGTCGCAGGTTGCACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGGTGAATGAACTGAGC‑GAAGAAGCGCTGATTCGGACCATCaaa < 2:210137/237‑1 (MQ=255) ggTCTGGGTAAAGTGATTACCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGc < 1:260512/239‑1 (MQ=255) tCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAACGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAGCAAGTTTGGTCTTATCCCTTAGTTTATTGGTCTACTTCCGGTTGTTGCCAAGTTGCAGCAACTGAGC‑GAAGAAGCTCTGATTCACATCCTAAAAGAGCCGCAAACCGcca > 2:364237/1‑238 (MQ=255) cTGGAAAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGccc < 1:1093492/238‑1 (MQ=255) tGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGCAGGCGAGCTGCTGGCGCAGGGTGAACCGGAAGATCTGATCAAGTTTGGGCTAATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAGTGAAAT‑AGCGGAATAAGCCCGGGTTCAGAACCCAAAACAGACAAAAAACGcccaa > 2:685890/1‑237 (MQ=255) ggATAAAGTGGTTTCCCTCCGGGTAGAAAGCGGGTCCGGCA‑TTGGTTTTGGCGCGTCGGTAAAAGCGAAGTGCGAAAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGACGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACTCCCTGa < 1:428283/239‑1 (MQ=255) | CACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTC‑GTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGC‑GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGA > NC_000913/457952‑458425 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |