BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,801	C→A	12.6%	A152S (GCG→TCG)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,801	0	C	A	12.6%	300.2 / 6.7	109	A152S (GCG→TCG)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (73/19); new base A (4/13); total (77/32)
Fisher's exact test for biased strand distribution p-value = 1.55e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 111 total aligned reads displayed.

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGG  >  NC_000913/4241563‑4242037

ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCAGCGAATA‑TG‑AA‑CTGGAGCCAGTTCCGc                                                                                                                                                                                                                                                  >  1:367538/1‑239 (MQ=255)

 cgggcgAAATCAGCCAGGGAGGACTTTCGGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCa                                                                                                                                                                                                                                                 <  1:635067/236‑1 (MQ=255)

 cgcgcgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCa                                                                                                                                                                                                                                                 <  1:165335/239‑1 (MQ=255)

   gacgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAat                                                                                                                                                                                                                                               <  1:673912/237‑1 (MQ=255)

    gcgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAATAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAat                                                                                                                                                                                                                                               <  1:377096/238‑1 (MQ=255)

     gcacctcggccaacGGGGACTTTCGGGCCCGGTAAAAGGCAGGCTTAAGAAAAGCAGTGCAGGGGTACATGCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCTGAACGGGCAATAAAATAGTTTACGCAGGAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAatat                                                                                                                                                                                                                                             <  2:662535/231‑1 (MQ=255)

           cagccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGGGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCACTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGAAA‑ACAGGTTAAAAAAGACACATTTGCCCG‑GAATA‑TG‑AA‑CTGGCACCATTTCCGAAAAAAACAtag                                                                                                                                                                                                                                       >  2:652595/1‑237 (MQ=255)

               caggGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCga                                                                                                                                                                                                                                   <  1:983092/239‑1 (MQ=255)

                     ggACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TA‑AG‑CTGGAGCCAGTTCCGCAAAATAAAATGGGGAGA‑G‑AAt                                                                                                                                                                                                                           >  2:153625/1‑239 (MQ=255)

                                 atggTTTACAGCAGCATTAAAACCAGCAGGGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAcc                                                                                                                                                                                                               <  2:1275962/237‑1 (MQ=255)

                                 accggataCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACCCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAAcc                                                                                                                                                                                                               <  1:223055/234‑1 (MQ=255)

                                        aCAGCAGCATTAAGACCAGCAGTGCAGGGATACATCCCGAGGCAAACATATAACGCAAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCAAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAACCC‑AACGCCa                                                                                                                                                                                                       <  1:1076259/239‑1 (MQ=255)

                                             agcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGGGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTAAGGCTAAACC‑AACGCCAATAc                                                                                                                                                                                                   >  1:118542/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATTCATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGc                                                                                                                                                                                                                     <  2:1228130/219‑1 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGCACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑ATGGAGCCAGTTACGCAAGAAAAATTGGCGAAA‑G‑ACATAAAGCAAAACC‑AACGCCAAAACCGc                                                                                                                                                                                                >  2:596308/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑CATGGTTAAAAGAGAA‑CAGTTTACCGCGCATA‑AG‑AA‑CTGGAGCCAGTTATGCAATATACATTGGC‑AGAGG‑CATTGAGGCCAAACC‑AACGCAAAAAcccc                                                                                                                                                                                                >  2:220053/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCCATATAAATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                 >  1:1254579/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGc                                                                                                                                                                                                                                     >  1:182338/1‑205 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTTAGGCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                 >  1:703605/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGc                                                                                                                                                                                                                     >  1:1228130/1‑219 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGc                                                                                                                                                                                                >  1:1092720/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAAAAccc                                                                                                                                                                                                 >  1:1181495/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑AATTGAGTCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                 >  1:671802/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATAAACATTGGCGAGA‑G‑CATTGAGGCCAAACC‑AACGCCAAAAACGc                                                                                                                                                                                                >  2:314613/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAAAA‑TA‑AG‑CTGGAGCCAGTTCCGCAATATAAATTGGCGAGA‑G‑CATTTAGGCTAAACC‑AAcccccaaaaccc                                                                                                                                                                                                >  2:376383/1‑231 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACCGGTTCAAAGAGAC‑CAGTTTCCCCCGAAAA‑TA‑AC‑CTGAACCAAGTTCCCAAATAAACATT‑GCGAGA‑GCCATTGAGGCGAAAca‑aaacccaaaacccc                                                                                                                                                                                                >  2:612351/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTAAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑CCTGGTTAAAAGAGAC‑CAGTTTCACGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACAATGGAGAGA‑G‑CATAGAAGCTAAACC‑AAAGCACATAccac                                                                                                                                                                                                >  2:490546/1‑237 (MQ=255)

                                                         agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGCGCCAGTTCCGCAATATACATTGGCGAGG‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCg                                                                                                                                                                                      >  1:86036/1‑239 (MQ=255)

                                                         agcagGGGAGGTAAACATTCGGGGGCAAAAATATAACGCCAGCCGTCAGTATTCAGCAAGCTGGCATCCCCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGAAA‑ACTGGTTAAAAGAGAC‑CAGTTCCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCg                                                                                                                                                                                      <  1:376383/239‑1 (MQ=255)

                                                              tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TA‑AG‑CTGGAGCCAGTTCCGCAATGAACATTGGCGCGG‑G‑CATTGAGGCTAAACC‑AACCCAAAAAACCCCAAAAATGCa‑aaaaa                                                                                                                                                                                >  2:822374/1‑239 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACa                                                                                                                                                                                                                                          >  2:81469/1‑183 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACa                                                                                                                                                                                                                                          <  1:81469/183‑1 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ataaata                                                                                                                                                                              >  1:542097/1‑239 (MQ=255)

                                                                cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ataaata                                                                                                                                                                              >  1:103974/1‑239 (MQ=255)

                                                                   ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑TTAATAAACa                                                                                                                                                                           >  1:398508/1‑239 (MQ=255)

                                                                     aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACaaa                                                                                                                                                                         >  1:855188/1‑239 (MQ=255)

                                                                        cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑TTAAATAACAAAtt                                                                                                                                                                       >  1:1002886/1‑238 (MQ=255)

                                                                        cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCCCCAATAATGCG‑ATAAAAAACAAAtt                                                                                                                                                                       >  1:1041871/1‑238 (MQ=255)

                                                                         aTTCCGAGGAAAAATAAAAACCCCAGCCGTAATTTTCCAGCCAGCTGGCATCACCGAAACGGGAAAAAAAATAGTTTACGAAGAAAACTAAAAGTTGCCCAAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTCCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTcc                                                                                                                                                                     <  1:965784/239‑1 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATGTAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑CTAAATAACCAATTCCGGGa                                                                                                                                                                 >  1:1125159/1‑239 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCAGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCTAGA‑G‑CATTGAGGCTATAAC‑AACGCCAATACCGCCAAAAATGCG‑ATAAATAACAAATTCCCGGa                                                                                                                                                                 >  1:551238/1‑239 (MQ=255)

                                                                               aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AAAGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGaac                                                                                                                                                               >  1:525734/1‑237 (MQ=255)

                                                                               aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AAAGCCAAGACCGCCAAAAATGAG‑aaaaaaaaaaaaatacaggaaa                                                                                                                                                               >  2:948985/1‑229 (MQ=255)

                                                                                           accGCCGGCCGTAAGTTTTCAGCCAGTGGGAACCCCCGGAACGGGAAAAAAAATAGTTTACGAAGTAAACTAAAAGTTGCCCAAAAATAATCGAAA‑ACTGGTTAAAAGAGAC‑CAGTTCCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                   <  1:568629/237‑1 (MQ=255)

                                                                                               ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGG‑G‑CATTTAGGCTAAACC‑AACGCAACTACCGCCAAT‑ATACGAATAAAAAAAAAATTCCCGAAACAAACCTGccggcaaaa                                                                                                                                               >  2:294803/1‑232 (MQ=255)

                                                                                               ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGCCGAGG‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTCCCAGATaaa                                                                                                                                               >  1:317517/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑TTAAATAACAAATTCCGGGACATAACCTGCCAGTTAAACAg                                                                                                                                            >  1:520245/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATTACAAATTCCGGGACATAACCTGCCAGATaacaag                                                                                                                                            >  1:1127185/1‑235 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAg                                                                                                                                            >  1:460648/1‑239 (MQ=255)

                                                                                                  gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAATTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTGCCGCAAAATCCATTGGCGAGC‑G‑CATTGAGGCTAAACC‑ACAGCCAACACCGCCAACAATGGG‑AAAAATAAAAAAATCAGGAAAAAAACCTGcacaaaaaaaag                                                                                                                                            >  2:506249/1‑228 (MQ=255)

                                                                                                        aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGAACAg                                                                                                                                      >  1:979025/1‑239 (MQ=255)

                                                                                                        aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAAA‑G‑CATTGAGGCCAAACC‑AACGCCACTACCGCCAATAATGCG‑ATCAATAACAAATTCCGGGACATAACCTTCCAGATAAACAGGCACAg                                                                                                                                      >  1:606504/1‑239 (MQ=255)

                                                                                                            ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGtgtt                                                                                                                                  >  1:1209728/1‑239 (MQ=255)

                                                                                                               agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTCCCAGATAAAAAGGCACAGtgttgt                                                                                                                                >  1:1132616/1‑238 (MQ=255)

                                                                                                                 ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CCTTGAGGCTAAAAC‑AACGCCAATACCGCCCAAAATGCG‑GAAAATAAAAAATTCCGGGGCACAACCCACCCGATAAACAGGAACAGAGTTGTACg                                                                                                                             >  2:890347/1‑239 (MQ=255)

                                                                                                                 ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGAACAGAGTTGTCCg                                                                                                                             >  1:801998/1‑239 (MQ=255)

                                                                                                                  aagcTGGAAACCCCGAAACGGGAAATAAAATTGTTTACGAAGTAACCAAAAAGTTGCCCAAAAATAACCGCAA‑ACTGGTTAAAAGAGCC‑CAGTTCCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTCCCGAAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCAAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                               <  1:314613/235‑1 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGt                                                                                                                          >  1:1075954/1‑239 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGt                                                                                                                          >  1:480457/1‑239 (MQ=255)

                                                                                                                     cTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAACAAGGCACAGAGTTGTCCGGGtt                                                                                                                         >  1:67368/1‑239 (MQ=255)

                                                                                                                                  aaCGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAAAGGaaaa                                                                                                             >  1:219343/1‑238 (MQ=255)

                                                                                                                                    cGGGCAAAAAAATTGTTTACGCAGTAAACTAAAAGTTGCCCGAAAAAAATCGCAA‑ACTGGTAAAAAGAGCC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCACAATATACATTGGCGAGA‑G‑CATTGAGGCAAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAAcc                                                                                                          <  1:948985/239‑1 (MQ=255)

                                                                                                                                                 aGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACg                                                                                                                                                                                                          >  1:513172/1‑131 (MQ=255)

                                                                                                                                                 aGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACg                                                                                                                                                                                                          <  2:513172/131‑1 (MQ=255)

                                                                                                                                                   tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  1:278132/1‑239 (MQ=255)

                                                                                                                                                   tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  1:610562/1‑239 (MQ=255)

                                                                                                                                                   tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  1:195066/1‑239 (MQ=255)

                                                                                                                                                   tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           >  1:888931/1‑239 (MQ=255)

                                                                                                                                                   tttACACAATAAACTAAAGTCTGCCAGAAAATAACCGAAA‑ACTGGTAAAAAAAGCC‑CTGTTCCCCGCGAATA‑GG‑GG‑GTGGAGCCAGTCCCGAAATATAAATTGGAGAAA‑G‑AGTTGAGGCTAACCC‑ACCGCAAATCCCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGc                                                                                           <  2:610562/239‑1 (MQ=255)

                                                                                                                                                    tttcccAGAAAAATAAAATTTGACAAAAAAAAAGCGAAA‑CCTGGTAAAAAGAAAC‑CATTTCCCTGCGAATA‑TG‑AG‑CTGGAGCCTGTCCCCAAATATACATTGGAGAGA‑C‑AATTGAGACAAACCC‑AACCCAATTACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  2:1092720/234‑1 (MQ=255)

                                                                                                                                                    ttccGCAGTAAACTAAAGTTTCCCCGAAAATAACCGCAA‑ACTGGTTAAAAAAAAC‑CGTTTCCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTCCCGCAATATACATTGGCGAAA‑G‑CATGGAGGTTAACCC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  1:652595/236‑1 (MQ=255)

                                                                                                                                                     ttaccatgtaaCTAAAAGTTTGCCAAAAATAAACGCAA‑TGTGGGTAAAAAGAAC‑CCGTTTCCCGGGGAAT‑TG‑TGCCTGGACCCGGTCCCGAATAATAGTTGGGCGAGC‑G‑ATTTGAGGCTAACCC‑AACGCCATTACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                          <  2:174448/230‑1 (MQ=255)

                                                                                                                                                     tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGCCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGGTTATAGAGGTACAACCAAGTTCTGGCCAGGCAg                                                                                         >  1:1128055/1‑239 (MQ=255)

                                                                                                                                                     tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAg                                                                                         >  1:688712/1‑239 (MQ=255)

                                                                                                                                                     tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAAAGGTAAAACCAAGTTCTGGCCAGGccg                                                                                         >  1:1284223/1‑237 (MQ=255)

                                                                                                                                                     tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGAGTTGTCCGGGTTTATAAAGGTAAAACCAAGGTCTGGCCAGGcgg                                                                                         >  1:1160346/1‑237 (MQ=255)

                                                                                                                                                     tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAACCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCCAAAACGCCAATAATTCG‑ATAAATAACAAATTCCGTGACCTCACCTCCCAGAAAAAAAGGCACAGTATTGTCCGGGTTTATAAAGGGAAAAACAAGTTCTGCCCAggaaa                                                                                         >  2:406767/1‑236 (MQ=255)

                                                                                                                                                       cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCgag                                                                                                                                                                                                                                  >  2:864408/1‑104 (MQ=255)

                                                                                                                                                       cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCgag                                                                                                                                                                                                                                  <  1:864408/104‑1 (MQ=255)

                                                                                                                                                        gCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGaa                                                                                       >  1:824524/1‑238 (MQ=255)

                                                                                                                                                           gTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAatat                                                                                                                                                                                                                                             >  2:50770/1‑89 (MQ=255)

                                                                                                                                                           gTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAatat                                                                                                                                                                                                                                             <  1:50770/89‑1 (MQ=255)

                                                                                                                                                             aaaCTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTGGTCCGGGCTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTac                                                                                  >  1:304938/1‑238 (MQ=255)

                                                                                                                                                                  aaaaATTGCCCCAAAAAAATTCCAATACTGATTAAAAGACAC‑TATTTACCCG‑GCATAGTG‑AG‑TTGGAGATAGTTCCGAAATATCAATTGGCGAAC‑G‑CATTGGGACTAAACC‑ATCGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                             <  1:1247777/239‑1 (MQ=255)

                                                                                                                                                                          cccGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCCGAAAAACAAGCACAAGGTTGTCCGGGTTTATAAAGGGAAAACCAAGTTCTGGCCAGGCGAAACCCACACCAGAaaaaaaaa                                                                    >  2:990320/1‑239 (MQ=255)

                                                                                                                                                                          cccGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATCAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaa                                                                    >  1:778087/1‑239 (MQ=255)

                                                                                                                                                                           ccGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTAGCCAGGCAGAACCTACACCAGAAATaaaaac                                                                   >  1:692442/1‑238 (MQ=255)

                                                                                                                                                                                aatacccGCAA‑ACTGGTTAAAACAGAC‑CGGTTCCCCGGAAATT‑TG‑GC‑GAGGACCCAGTGCCGAAATATCACGTGGGGAGA‑G‑CTTTGGGTCCACACC‑GACGCCAAATCCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                              <  2:756084/233‑1 (MQ=255)

                                                                                                                                                                                   aatCGAAA‑ATTGGTTAAAAGAGAC‑CAGTTCCCCG‑GAATA‑TGTGG‑GTGGGCCCAGTTCCGCAATAACCATTGGCGAGA‑A‑CATTGAGGCTAACCC‑AACCCAAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcag                                                           <  1:596308/239‑1 (MQ=255)

                                                                                                                                                                                      caaaa‑aCTGGTAAAAAGAGAC‑CATTTCCCCGCAAATT‑GG‑GG‑CTGGAGCCATTTCCGAAATATACATGGGCGAAA‑G‑CATTGAGGCAAACCC‑AACGCAAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                        <  1:890347/236‑1 (MQ=255)

                                                                                                                                                                                                          aC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCACTACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGCTAACCAGGCCCAGTGTTGTCCGGGTTTATCGCGGTAAAACCAAGTCCTGACCAGGCAATCCCTCCCCCAGAACAAAAACAAAGAAACACACCAATCTTaacggaaaccaa                                     >  2:616519/1‑229 (MQ=255)

                                                                                                                                                                                                          aC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAATTGAATCacg                                     >  1:242977/1‑239 (MQ=255)

                                                                                                                                                                                                          aC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                     >  1:805562/1‑239 (MQ=255)

                                                                                                                                                                                                                tttCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACgacgac                                >  1:614581/1‑239 (MQ=255)

                                                                                                                                                                                                                   cccGCGAATA‑TG‑AG‑ATGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATATGGGTAAAACCAAGTTCTGGCCAGGAAGAACCTACACCAGAAATAAAAAACAGGACAGCACCAATCTTAGGGGAATAACGACGACCGa                             >  1:8289/1‑239 (MQ=255)

                                                                                                                                                                                                                         aaTA‑GG‑AG‑CGGGACCCATTCCCGAAATATACATTGGCGAGA‑G‑AATTGAGGCTAACCC‑AACGCAAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                       <  1:999124/239‑1 (MQ=255)

                                                                                                                                                                                                                          ata‑tG‑AG‑GTGGAGCCAGTCCCGAAAATTACTTTGGGGAGA‑C‑AATTGAGGCTAAACC‑AACCCAAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                       <  1:635605/238‑1 (MQ=255)

                                                                                                                                                                                                                                      gaaaccggTTCCAAAATATGAATTGGGGAGA‑G‑‑ATTGGAGGTAAACCAAACGCCAAAACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAGAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAAt              <  1:406767/231‑1 (MQ=255)

                                                                                                                                                                                                                                            aGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTAAACCCGAAATAAAAAACAGGCCAGAAGCAATCTTAAGTGAATCACAACGACCGAACAGGTTACAGAAAAAaccaa        >  2:1023414/1‑237 (MQ=255)

                                                                                                                                                                                                                                                 cGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAGATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTACGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAACAATCTTAATGAAATCACGACAACCAAAACGATAAATGCAAAAAAAACCAAggg  >  2:490370/1‑239 (MQ=255)

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAA‑ACTGGTTAAAAGAGAC‑CAGTTTCCCGCGAATA‑TG‑AG‑CTGGAGCCAGTTCCGCAATATACATTGGCGAGA‑G‑CATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCG‑ATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGG  >  NC_000913/4241563‑4242037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`