| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 37,250 | A→C | 21.8% | V192G (GTG→GGG) | caiC ← | putative crotonobetaine/carnitine‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 37,250 | 0 | A | C | 21.8% | 113.4 / 3.8 | 65 | V192G (GTG→GGG) | caiC | putative crotonobetaine/carnitine‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (26/20); new base C (3/15); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCG > NC_000913/37015‑37475 | cgtattACGTTCGGTACGGGGGGCCCGTGACTTTTGGTCCTGTCCCCCGGAGGCGGGGGCTCTTTTTTTCCCGACCAGCACAAAGGTGGCCTCGGCAGAAAACGTCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATcacca < 2:259245/234‑1 (MQ=255)cGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTAATTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATcacca < 1:510981/239‑1 (MQ=255) tCGGTAACGGTGGCGCGGTTCTTCCGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTtcgg < 1:494595/239‑1 (MQ=255) gTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGGGCAGGTTGTAATGGGTAATCACCCAACCTTtcggtcg > 1:139061/1‑239 (MQ=255) aaCGGTGGGGCTGTACTTCTGTACCGTTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCCAAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGGGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCggg < 2:562892/239‑1 (MQ=255) cGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAAAATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGCAg > 1:39172/1‑239 (MQ=255) gcgcGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTACTATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCCCACCTTTCGGTCGGGCggtggg > 1:634360/1‑238 (MQ=255) gcgGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAAAGCAGGCCTTACCGCCAGGTACACG‑TCATCGTCCCGCAGTGCACACTCCCAGGCGGAGTAATATCAAGCGAACCGCAGGTGGTAATGGGGAATACCAACACCTTTCGGTCGGGAgggggggg > 2:436227/1‑236 (MQ=255) ttCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGt < 1:370842/239‑1 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGGGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGGGGTGGTGCCGGAGGTGAAAGGAAtt > 1:687246/1‑239 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAAtt > 1:501685/1‑239 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGGGAGGAGAAtt > 1:510286/1‑239 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATACCCACACCTTTCGGTCGGGAGGGGGGGCCGgggggggaaggaaat > 2:52007/1‑228 (MQ=255) ccccAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCAAGCACTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTAGTAATGGGTAATCACCACACCTTTCGGACGGGGGGTGGGGCGGGGGGGGAAGAGAAGTTc > 2:122110/1‑239 (MQ=255) cccAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTc > 1:150734/1‑238 (MQ=255) cAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGGAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTccg > 1:484423/1‑238 (MQ=255) aaGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGt > 1:788140/1‑239 (MQ=255) gcgcGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGGGCCGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGGGGGGGGGCCGGAGGGGGAGAGAAGTTccgccggagg > 2:452554/1‑235 (MQ=255) gggCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGGGCACACTGCCAGGCGGAGTAAAATCCAGCGAAGACCAGGTTGAAATGGGAAATCCCCACACCTTTCGGTCGGGGGGCGGGGCCGGAGGTGAAGAGAAATTCCACCATATCGtaa > 2:260018/1‑237 (MQ=255) ctcGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGAAATCACCCAACCTTTCGGTCGGGAGGTGGTCCCGGAGGTGACGAGAATATCCGCAGTATCGTCAGTCGATAgcgtcg > 1:847176/1‑239 (MQ=255) ctcGACCAGCAAAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAgcggcg < 1:715794/239‑1 (MQ=255) ccaccaCAAGGGGGGCCCCGGCAGAAACCGCCGCCATCGCCGCAGTCCACGGGGAACCGATTGAAAACCCGGGATTTACCGGAAGGT‑GACGTTCATCGGCACGCAGTGCACCCTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCCCACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 2:24123/239‑1 (MQ=255) ccaccaCAAAGGGGGCCCCGGAAAAAACCGCCCCCATCGCCGCAGTACACTGGCAATCGATAGGAAACGCAGGCATTACCGTCAGGTAGGCG‑TCATCGTCACGCAGTGCACACTGCCGGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAAGGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 2:139061/239‑1 (MQ=255) ccAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAAGGGAAGAGAATTTCCCCCGGATCGTCAGTCGAAACcggcgggggc > 1:625328/1‑235 (MQ=255) ccccGGCAGAAAACGCCGCCAGCGCGGCAGTACACTGGAAATCGTTATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACGGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGc < 1:298931/238‑1 (MQ=255) cgccATCGCCGCAGTACACTGGCAAGCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCAAACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCa < 1:50758/239‑1 (MQ=255) aTCCCCGCAGTACCCGGGCAATCGATAGGAAACGCCGGCTTATCCGTCAGGTAGCGG‑TCCCCATCACGCAGTGCACACTGCCAGGCGAAGTAATATCCAGGGAAGCGCGGGTTGTAAGGGGTAATCACCACCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTg < 2:510286/239‑1 (MQ=255) cgcAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCGTAGGACAAGGGGGCAGGTTTGTGA‑TTTTTTAGTTGAggga > 1:823867/1‑236 (MQ=255) aTCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCGGGGTGTTGG‑GTTTTTAGTTGGGTCAACAGACTccccccc > 1:162099/1‑232 (MQ=255) aTCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGGGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCCTAGCCCAAGGGGGCCGGTGTTTGAGTTTTTCTTTTG‑GTAAACGAACTCACGccc > 1:188641/1‑237 (MQ=255) aGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGGACAGCCCAAAGGGGCAGGGTGTTGA‑TTTTTTAGTTGAGTAAACGAACTCACGCCCTC‑ATCAGCGGGAAATGCCCAATCTGTccg > 1:99090/1‑237 (MQ=255) ggcATCGTCACGCAGTGCACACGGCCAGGCGGAGTAATATCCAGCGAAGGGCAGGTTGTAATGGGTAATCCCCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAAtg < 1:100717/236‑1 (MQ=255) tCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGCATAGCACAAGGGGGCAGGGTGTTGA‑TTTTTCTGTTTAGTAAAAGAAATCACCCCACC‑ATCAACGGGGAGTGGCACAACTGTCAGGaaaaaggggcg > 2:646764/1‑232 (MQ=255) tcccGCAGTGCACACTGCAAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑Caa < 1:680417/236‑1 (MQ=255) aCGGAGGGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAAGGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAAtt < 1:421/239‑1 (MQ=255) gcgtgccactcgCCAGGGCGAGTAATTCCCCGCGGAGGGCAGGTTGAAATGGGTAACCCCCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCAAAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAg < 1:242298/228‑1 (MQ=255) catgtcaccCTGCCGGGGGGAGTAATTTCCAGCGAAGCTCAGGTTGTAATGGGTAAACCCCAACCCTTTCGGGCGGAAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAg < 1:377379/234‑1 (MQ=255) tGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGCATAGAACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATAAGCGGGGAAGGCCACATCTGTTAGGGAAATGTGCCG‑CAATTGGGTGGGATAtt > 1:873661/1‑238 (MQ=255) gCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGCAGAGAATTTCCGCCGTAGCGTCCGTCGATAGCGGCGGTGCAGAGCACAAAGGGGCAGGTGTTTGT‑TTTTTTAGTTTGGGAAAGCAACCACCGCCAAC‑ATTACCGGGGAGGGCTAAATCTTTTCGGCAAAAGTTCCGCCAATTG‑GGGGCATCtcc > 2:836281/1‑236 (MQ=255) gcgggtttttttCCAGGTAAGCTCAGGTTGTAAGGGGTAATCCCCCCCCCTTTCGGGCGGGGGGTGGTGCGGGAGGTAAAGGGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCctgct < 2:99090/227‑1 (MQ=255) aGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATTTGCCG‑CAATTGAGTGGGACTTTCCTGCTGaa > 1:410456/1‑237 (MQ=255) aGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGGTGTTGA‑TTTTTCAGTTTAGTAAACGAACTCACGCCACC‑ATCAGCGGGAAGGGCCACATCTGTAAGGCAAAAGTGCCG‑CAATTGAGGGGGATCTTCCTGCTGGAta > 1:289945/1‑238 (MQ=255) aGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGACGATAGCGGCGGGGCCCAGACAAAGGGGGCAGGTTGGTGA‑TTTTTCCATTGAGTTAACGAAATCAAGCCACC‑CACCACAGGAGGTGCCCCACCTGTCAGGCAAAAGTGCCG‑CAATTGAGGGGCGACTTACTGCTGaaaa > 2:44401/1‑237 (MQ=255) ggaaattCCAGCGAGGGGCAGTTTGTATTGGGTAATCCCCACACCTTGCGGTCGGGAGGTGGTGCCGGAGGTAAAGGGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACAGCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATc < 1:415831/232‑1 (MQ=255) gTAATTGCCAGCGAAGCGAAGGTTGGAATGGGAAATCACCCCCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATc < 1:260018/238‑1 (MQ=255) atttCCAGGGAAGCGCAGGTTGAAATGGGTAATCACCCCCCCTTTCGGTCGGGAGATGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGt < 1:436227/235‑1 (MQ=255) cccAGCGAAGCTCAGGTGGTAAGTGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGc < 1:190951/201‑1 (MQ=255) ggggAAGCGGAGGTTGTAAGGGGTAATCCCCCCCCCTTGCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:339987/236‑1 (MQ=255) ggggAAGCGCAGGTTGTAATGGGTAATCACCACCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:452554/236‑1 (MQ=255) gcgggggggcAGGTTGTAATGGGTAATGCCCCCCCCTTTCGGTGGGGAGGGGGTGGCGGGGGTGAAGAAAATTTCCGCCGTTTCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:873661/231‑1 (MQ=255) cgggaggcggAGTTTGTAATGGGTAATCCCCCGCCCTTGCGGGCGGGGGGTGGGTCCGGGGGTGAAGAAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:410456/233‑1 (MQ=255) cgcggaggggaGGTTGAAAGGGGTAATCCCCACCCTTTTGGGTCGGGGGGTGGGGCGGGGGGTAAAGGGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:447256/229‑1 (MQ=255) aGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGGAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCCCATCTTTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTTGTACa > 1:400776/1‑239 (MQ=255) aGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGGGGATAGCCCAAGGGGGGCGGGTGTTGA‑GTTTTTAGTTGAGTAAACGAACTTCCGCCAACAACCACCGGGAGGTGCC‑CCTCTGTCCGGGCAAAGTGGCG‑CCAATTTGTGGCCTCTCCCTTCCGAATCTGTTgtataa > 2:292120/1‑234 (MQ=255) gaaggggggtttGTAAGGGGTAATCACCCCCCCTTTCGGGCGGGAGGGGGTGCCGGGGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:150734/226‑1 (MQ=255) gAAGCGCAGGTTTTAATGGGTAACCCCCCCCCCTTTCGGTCGGGAGGGTGTGCCGGAGGTTAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:368109/236‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAAGTGAAGAGAATTTGCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:1267/236‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAAGCACCCCACCTTTGGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:572510/236‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAACCTCCACCCCGTTCGGGCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:351381/236‑1 (MQ=255) gAAGCGCAGGTTGAAATGGGTAATCCCCACCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:52007/236‑1 (MQ=255) gAAGCCCAGGTTGAAATGGGTAATCACCACCCCTTTGGGGCGGGAGGGGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:113281/236‑1 (MQ=255) gccgagttgttAAGGGGTAATCCCCCCTCCTGGCGGGCGGGAGGTGGGGCCGGGGGTGAGGAGAATTGCCGCCGTAACGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGa < 2:188641/228‑1 (MQ=255) cgcAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGGTGTTTA‑TTTTTCAGTTGGGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGGGCCCCATTTTTCAGGCAAATGTGCCC‑CAATTGAGTGGCATCTTCCTGCTTAATCTTTTGATAACTAGGATa > 1:447256/1‑239 (MQ=255) ggaGGTTGAAATGGGTAAACCCCACCCCTTTCGGTCGGGAGGGGGGCCCGGAGGTGAAGAGAATTGCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATa < 1:451267/236‑1 (MQ=255) ggtttttAATGGGTAATCCCCACACCTTGCGGTCGGGAGGTGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGaa < 2:289945/233‑1 (MQ=255) gtttaagtggTAAGCCCCACCCCTTTCGGGCGGGAGGTGGGGCCGGGGGTGAAGGGAATTTCCGCCGGATCGGCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAAtt < 1:321965/230‑1 (MQ=255) gTAATGGGAAATCACCACACCTTTCGGTCGGGAGGTGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTgcg < 1:63613/239‑1 (MQ=255) | CGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACG‑TCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAATCACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGA‑TTTTTCAGTTGAGTAAACGAACTCACGCCATC‑ATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCG > NC_000913/37015‑37475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |