Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 830,865 A→G 19.7% intergenic (‑222/‑7) ybiH ← / → rhlE DUF1956 domain‑containing tetR family putative transcriptional regulator/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913830,8650AG19.7% 129.8 / 5.7 74intergenic (‑222/‑7)ybiH/rhlEDUF1956 domain‑containing tetR family putative transcriptional regulator/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base G (14/2);  total (46/28)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCACGCCAAAGGGCGTCGTCCGGTACG  >  NC_000913/830627‑831101
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
aTGGCAGGATTATTCATCGGACAGTCCATTCTTAATCAAATGAGTGATTAAGATTATGCCTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGa                                                                                                                                                                                                                                                  <  1:358607/239‑1 (MQ=255)
aTGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGa                                                                                                                                                                                                                                                  <  1:39933/239‑1 (MQ=255)
        attattCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTAAACCGGTAACGGTCGGGGCGGTTCGGGGTAGTTAt                                                                                                                                                                                                                                          >  1:199642/1‑239 (MQ=255)
               aTCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGGAATTATGTCtttt                                                                                                                                                                                                                                   >  1:515565/1‑238 (MQ=255)
                 cGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGCCTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGa                                                                                                                                                                                                                                 <  1:392971/239‑1 (MQ=255)
                 cGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGa                                                                                                                                                                                                                                 <  1:780745/239‑1 (MQ=255)
                 cGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGGAGGTCTATCTTTTGa                                                                                                                                                                                                                                 >  1:392627/1‑239 (MQ=255)
                        tCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGGTTTTGTCATTTTCCTTTTCAGTATCTCCCTGCAAACTACACCGGTAACGGTCGGGGCGGTTCTGGGGAGATATGTTTTTTCATACtttt                                                                                                                                                                                                                          >  2:516656/1‑238 (MQ=255)
                             tCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGCGGTAGGTATGTCTTTCGCGTCTTTGGGttt                                                                                                                                                                                                                     >  1:592445/1‑239 (MQ=255)
                             tCTTAATCAAATGATTGATTAAGAATATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTGTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGttt                                                                                                                                                                                                                     <  1:423820/239‑1 (MQ=255)
                              cTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTa                                                                                                                                                                                                                   <  1:217354/239‑1 (MQ=255)
                                  aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAACCTACACCGGTAACGGTCGGGGCGGTTTCGGTAAGGTTTGTTTTTTGATTCTTTGGGgtttatg                                                                                                                                                                                                                 >  2:870700/1‑234 (MQ=255)
                                  aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTTCGAAGAAATTTTTCTTTCGGTTCTTTGGGTGTAAAcc                                                                                                                                                                                                               >  2:336445/1‑239 (MQ=255)
                                  aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGTGATTATGTCTTTCGATTCTTTTGGTTTaatc                                                                                                                                                                                                                >  1:210484/1‑236 (MQ=255)
                                  aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGTAGTTATGTCTTTCGATTCTTTGGGttttaac                                                                                                                                                                                                                 >  1:875684/1‑234 (MQ=255)
                                  aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGGTTAAGc                                                                                                                                                                                                                >  1:88861/1‑238 (MQ=255)
                                                    aTTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTTTGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTCCGCGCCg                                                                                                                                                                                           >  1:619573/1‑239 (MQ=255)
                                                       aTGACTCCATGGGGGAGTTGTCCAGTAGGGCTAAGAATTTTAGCAACGCCAGTACCAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGGTCCCTCATGACAGTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGAGGCGGTTCTGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTg                                                                                                                                                                                        <  1:824526/239‑1 (MQ=255)
                                                        tGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAACCTACACCGGTAACGGTCGGGGCGGTTCGGGGGAGTTATGTCTTTCGATTCTTTGGGGTTTAGCCCCGTTATCCGTCCCGCCGTTGc                                                                                                                                                                                       >  2:546435/1‑239 (MQ=255)
                                                        tGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGTTGTTATGTCTTTTGTTTTTTTGGGGTTTAGCCCTGTAATCCTGGGCGCCGTGGc                                                                                                                                                                                       >  2:629858/1‑239 (MQ=255)
                                                        tGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGGAGTTATGTCTTTCGATTCATTGGGTTTAAGCCCCGATATCCTGCGCCCCGttt                                                                                                                                                                                        >  1:165119/1‑237 (MQ=255)
                                                              cATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTTGGGTTAAGCCCTGATATCCTGCGCGCCggttgcgggca                                                                                                                                                                                 >  2:196618/1‑229 (MQ=255)
                                                              cATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGGTTGGAGGAGTTTTGTCTTTTGATTCTTTGGGTTTAAAGCCCCGAATCCCTGCGCCCCTTGCCggacg                                                                                                                                                                                >  2:860524/1‑235 (MQ=255)
                                                                      aGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACCCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGGTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGAGATCCTTCGCGCCGTTGCCGAGCCAGGTTAcc                                                                                                                                                                        >  1:459873/1‑239 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTTGGGGTAGTGTTTTCCTTTGATTCTTTGGGTTTCAACCCTGATAATCTGCCGGCCGTTGCCGGGGCAGGGTTAcgg                                                                                                                                                                       >  2:816115/1‑237 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGTGAGTTATGTCTTTCGATTCTTTTGGTTTAAGCCCTGATATCCTGCGCGCCGGTTCCGGGCAGGGGTACCGt                                                                                                                                                                      >  1:393741/1‑239 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATACTGCGCGCCGTTGCCGAGCAGGGTTACCGt                                                                                                                                                                      >  1:392486/1‑239 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTGTTTATGTCTTTCTATTCTTTGGGTTTAAGCCCCGATTACCTCCTCGCCGTTCCCGAGCCGGGTTAcccg                                                                                                                                                                      >  1:877086/1‑237 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTTTGTCTTTCGATTCTTTGGGTTTAAGCCCGGATATCATGGGCGCCGGTACCGACACGGGTTTCCGt                                                                                                                                                                      >  1:734294/1‑239 (MQ=255)
                                                                        ttGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGGTACCgg                                                                                                                                                                      >  1:297905/1‑238 (MQ=255)
                                                                             ctgtttGGCTAAAAATTTTAGCCACGCCAGTCACAGGGTTAATTTATGCGCTGCGTCCCAAAAACTGCTACCCTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAacc                                                                                                                                                                 <  2:81911/234‑1 (MQ=255)
                                                                             ccgttgggttAAGAATTTTAGCAACGCCAGTCACAGGGTTAATTTATGCGTTGCGGCACAAAAACTGCTACACCCCGCTCCCTCATGACATTGTTGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAacc                                                                                                                                                                 <  2:601267/230‑1 (MQ=255)
                                                                             cAGTTTTGCTTAAAATTTTAACAACGCCAGTTCAAGGGATTATTTATGCGTTGCTTCAAAAAACCTGCTACACCCCGGTCCCTCAAGAAATTGTGGTTTTTGGCATTTTCCTTTTCAGTATCTCCCTTAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAacc                                                                                                                                                                 <  2:875684/239‑1 (MQ=255)
                                                                                  tGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGGTTCTTTGTGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCCAGCAGGGGTACCGGGAacccaac                                                                                                                                                             >  2:891171/1‑236 (MQ=255)
                                                                                  tGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAacccccc                                                                                                                                                             >  1:81911/1‑238 (MQ=255)
                                                                                     cTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTTGGAGTAGTTATGTCTTTCGGTTCTTTGGGGTTAAGCCCTGAAGTCCTTGCGCCCGTTTCCGACGAAGGTTTCCGTTAACCCAccccaa                                                                                                                                                         >  2:616109/1‑237 (MQ=255)
                                                                                     cTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGGTTAAGCCCTGATATCCTGCGCGCCGTTGCCGGGCAGGGTTACCGTGAACCCAACCCtt                                                                                                                                                         >  1:793381/1‑238 (MQ=255)
                                                                                        aGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGGTCGGAGTTGTTATGTCTTTTGGTTCTTTTGGGTTTAGTCCTGATTTACGTGCGCCCGTTGCCGGGGCGGGGTTCCGGCAACCCCCCCCTATTc                                                                                                                                                      >  2:505913/1‑239 (MQ=255)
                                                                                          aaTTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGGTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCCATTccg                                                                                                                                                    >  1:730393/1‑237 (MQ=255)
                                                                                                      gCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCCGGGTTACCGTGAAACCACACCTATTCAGCAGCAGGCggt                                                                                                                                         >  1:81302/1‑236 (MQ=255)
                                                                                                          gtccaaGGTATTATTTTTGTGTTGCTTCAAAAAAACTGCTACCCTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTg                                                                                                                                    <  2:297905/234‑1 (MQ=255)
                                                                                                                    tAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTACGCCCTGTTGTCCTGCGCGCCGTTGCCGGGCAGGGTTACCGGGAACCCACCCCTATTCAGCCGCAGGCGCTCCCTTCGGGGCTGGa                                                                                                                          >  2:729646/1‑239 (MQ=255)
                                                                                                                          aTGCGCTGCGTCTCAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCcg                                                                                                                    <  1:73417/239‑1 (MQ=255)
                                                                                                                           tGCGCTGCGTCACAAAAACTGCTTCCCTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCcgc                                                                                                                   <  1:816115/239‑1 (MQ=255)
                                                                                                                           tGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACGCACCCCCATTCAGCACCAGGCGATCCCTCCGGGGCTGGAAGGCcgc                                                                                                                   >  1:391680/1‑239 (MQ=255)
                                                                                                                            gcgcTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTTGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGGTATCCTGCGCGCCGTTTCCGCGCAGGGTTACCGGGAACCCCCCCCTCTTCAACAGCCGGCGAACCCCGCGGGGGTGGAAagcagc                                                                                                                   >  2:44231/1‑238 (MQ=255)
                                                                                                                            gcgcTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGCTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTTAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGGTACCGTGAACCACCCCCCATTCAGCAGCAGGCGATCCCTGCGGGGCTCGAAGccccc                                                                                                                   >  1:728849/1‑236 (MQ=255)
                                                                                                                                cgCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGGAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTTCGCGCCGTTGCCGGGCAGGGGTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTCCTGGAAAGcccggc                                                                                                                 >  1:561487/2‑232 (MQ=255)
                                                                                                                                  cGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTTGGTTTAAGCCCTGGTATCCTGCGCGCCGTTGCCGAGCCGGGGTACCGTGGACCCCCCCCTATTCCGCAGCCAGCGGACCCTGCCGGGGTGGAGGGCCGCGccccaa                                                                                                            >  2:639880/1‑236 (MQ=255)
                                                                                                                                  cGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAg                                                                                                                                                                                  >  1:477402/1‑169 (MQ=255)
                                                                                                                                  cGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAg                                                                                                                                                                                  <  2:477402/169‑1 (MQ=255)
                                                                                                                                  cGTCACAAAAACTGCTACACTCCGCTCACTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGGAGTTATGTCTTTCGATTCTTTCGGGTTAAGGCCTCACATACTTCGCGCCGTTGTCCAGCAGGGTTACCGTGAACCCCACCCCACTCCGCAACAGGCGCTCCCCGCGGTGCTGGAAAGCcgcgcacaga                                                                                                            >  2:125671/1‑233 (MQ=255)
                                                                                                                                  cGTCACAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAg                                                                                                                                                                                  >  1:198892/1‑168 (MQ=255)
                                                                                                                                  cGTCACAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAg                                                                                                                                                                                  <  2:198892/168‑1 (MQ=255)
                                                                                                                                       caAAAACTGCTACCTTCCTCTCCCTCATGACATTGTGGTTTTTGGCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCt                                                                                                       <  1:305073/239‑1 (MQ=255)
                                                                                                                                                      tCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTTCCGAGCAGGGTTACCGGGAACCCCCCCCCATTCCGCCGCCGGCGGTCCCCTCGGGGCCGGAAAGCCGCGCACTCATTGCTACGccccacacccg                                                                                        >  2:105534/1‑230 (MQ=255)
                                                                                                                                                      tCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGaccggc                                                                                        <  1:806552/239‑1 (MQ=255)
                                                                                                                                                        cccccccTCATGACATTGTGGTTTTTGTCATTTCCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGaccggcac                                                                                      <  2:730393/235‑1 (MQ=255)
                                                                                                                                                        cGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCCATTCAGCAGCAGGCGATCCCCGCGGTGCTGGAAGGCCGCGACCTGCTGGCTAGGGCCCCGacccgca                                                                                       >  1:614432/1‑238 (MQ=255)
                                                                                                                                                        cGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGGTTAAGCCCTTATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCCCCCCCATTTCGCCGCCGGCGATACCCTCGGGGCTGGAAGGCCGCGGCCTGATGGGTAACGCCCAGacatcccc                                                                                      >  2:94770/1‑233 (MQ=255)
                                                                                                                                                                   gACATTGTGGTTTTGGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAg                                                                           <  1:616109/239‑1 (MQ=255)
                                                                                                                                                                    aCATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGTAGTTATGTCTTTTGCTTCTTTGGGGTTAAGCCCTGATATCCTGCCCGCCGTTTCCGAGCAGGGTTACCGGGAAACCACCCCTCTTCAGCAGCCGGCGATCCCCGCGGGGTCGGAAAGCCCCCACCCGATGGGCACGGCCCCAACCCGCACCGCCCAAACaac                                                                          >  2:615383/1‑237 (MQ=255)
                                                                                                                                                                        tgtgGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGGGCTGGAAGGCCGCGACCCGATGGCTAGCGCCCAGACCGGCACCGGCCAAACAGCgg                                                                        >  1:209714/1‑237 (MQ=255)
                                                                                                                                                                         gtgGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGGGGAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTTCGCGCCCTTTCCCAGCCGGGTTACCGTTGACCCACCCCTTTTCAGCAGCAGGCGATCCCCGCGGGGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCCCCCGCAAAAAACCggggt                                                                     >  2:357799/1‑237 (MQ=255)
                                                                                                                                                                             tttttGTCATTTTCCTTTTCAGTAGCTCCCTGAAACCTACTCCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTa                                                                  <  1:539302/238‑1 (MQ=255)
                                                                                                                                                                                  gTCCTTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGGCGGGGCGGTTCGGAGTAGTTAGTTCGTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGc                                                            <  1:443920/239‑1 (MQ=255)
                                                                                                                                                                                  gTCATTTTCCTTTTCAGTTACCTCCTGAAAACTACCCCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGc                                                            <  1:742827/239‑1 (MQ=255)
                                                                                                                                                                                     attttcattttcAGGATCCCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATTTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGc                                                         <  1:10344/239‑1 (MQ=255)
                                                                                                                                                                                      ttttCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGAGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCCCCGTTGCCGCGCAGGGTTACCGTGGACCCCCCCCTATTCAGCACCCGTCGGTCCCTGCCTGGCCGGAAGGCCCCCCCCTGATGACTAGGGCCCCAACACGCACCCGGCAAACAGCGGGCCTTAAGcgcccacg                                                        >  2:881819/1‑232 (MQ=255)
                                                                                                                                                                                           cTTTTCAGTATCTCCCTGAAAACTACGCCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGc                                                   <  1:860524/239‑1 (MQ=255)
                                                                                                                                                                                                                   tccccGGGAACGGTCGGGGGGGTTTGGGGTTGTTTTGTCTTTCGGTTCTTTGGGTTTAAGCCCTGATTTCCTGCGGGCCGTTGCCGAGCCGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGc                           <  2:210484/236‑1 (MQ=255)
                                                                                                                                                                                                                   accccGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGc                           <  1:196618/236‑1 (MQ=255)
                                                                                                                                                                                                                    cccaggTAACGGTCGGGGCTGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCGGTGGCCGAGCAGGGTTACCGTGAACCCACCCCTATGCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCa                          <  1:435407/235‑1 (MQ=255)
                                                                                                                                                                                                                           aaCGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCACGCCaa                    <  1:891171/238‑1 (MQ=255)
                                                                                                                                                                                                                                gcGGGGGGGTTTGGGGTTTTTTTGTTTTGCGATTCTTTGGGTTTAAGCCCGGATATCCTGCGGCGCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGGCCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCACGCCAAAGGGc               <  2:793381/237‑1 (MQ=255)
                                                                                                                                                                                                                                             ggAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATTTCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCCCCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCACGCCAAAGGGCGTCGTCCGGTACg  <  1:885699/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
ATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCTTTTCAGTATCTCCCTGAAAACTACACCGGTAACGGTCGGGGCGGTTCGGAGTAGTTATGTCTTTCGATTCTTTGGGTTTAAGCCCTGATATCCTGCGCGCCGTTGCCGAGCAGGGTTACCGTGAACCCACCCCTATTCAGCAGCAGGCGATCCCTGCGGTGCTGGAAGGCCGCGACCTGATGGCTAGCGCCCAGACCGGCACCGGCAAAACAGCGGGCTTTACGCTGCCGCTGTTGCAACACCTGATCACTCGCCAGCCGCACGCCAAAGGGCGTCGTCCGGTACG  >  NC_000913/830627‑831101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: