Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,877 A→G 25.4% E176G (GAG→GGG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,8770AG25.4% 80.3 / 2.2 47E176G (GAG→GGG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (8/24);  new base G (11/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 4.97e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTT  >  NC_000913/71650‑72113
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGTCTGCCGGCTGTGGCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGCTATTCCTGCCGGACTGCAGAGCGGGGGGATTAAATGGt                                                                                                                                                                                                                                     >  2:260966/1‑239 (MQ=255)
aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTTTTACGCCGAATATTATCGGCGGCCTGCTGGGGCCGCCCTTTTACTTCCTCCCAGGGATTCTGGCGGGGCCGGCGCTCGGTATTCCTGCCGGAAGGGAGAGCGGGGAGGTTAAAAggg                                                                                                                                                                                                                                     >  2:204918/1‑238 (MQ=255)
  cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGGGGCCGCCGGTTTACTTCCTGCCAGGGTTTCTGGGGGGCGACGGGGTCCGATTTCCTTCCGGACAGCCGAGCGGGGGGTTTAAAGGGTtg                                                                                                                                                                                                                                   >  2:105587/1‑239 (MQ=255)
     aaCATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCGGCCAGGGATTCTGGAGGGCGCGGGGTTCGGCATTCCTCCCGGAATGCAGAGCGGGGCGTTTAAATGGTtgct                                                                                                                                                                                                                                 >  1:297963/1‑238 (MQ=255)
           cGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTCAATTTTTTACCCCGAATCTTATCGGCTGCCTTCCTTGGCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGCTCGCATTTTCTGCCGGAATGTAGGGCGGGGGGTTTAAAAGGTTGCTGCGGGCa                                                                                                                                                                                                                          >  2:436346/1‑239 (MQ=255)
               gCATCAACCCAGCATGGTCACCATTCTGGTGGGTCGTTTTGTTGGCCCGACGTGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAg                                                                                                                                                                                                                      <  1:450098/239‑1 (MQ=255)
               gCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGAGCGGTATTCCTTCCGGAATGCAGAGCGGTGAGGTTAAATGGTTGCTGCTGGGAAAAg                                                                                                                                                                                                                      >  1:830574/1‑239 (MQ=255)
                          gCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTGCCGGAATGCAGAGCGGGGGGGTTTAATGGTTTCTGCCGGCCAAAGCGGGGgttttt                                                                                                                                                                                                          >  1:537419/1‑238 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGGATATTATCGGCTGCCTGCTGTTGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGCTCGCTATTCCTCCCGGGATGCAGCGCGGGGGGGTTAAATGGGTGCGGCTGGGAACCGCGGGGTGTTtgt                                                                                                                                                                                                         >  2:84490/1‑239 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGGTATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGGTTTtgt                                                                                                                                                                                                         >  1:897135/1‑239 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGAGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGCTATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTtgg                                                                                                                                                                                                         >  1:199096/1‑238 (MQ=255)
                                tCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTTTGGCGGGCGCGGCGGTGGATATTCCTTCCGGAATGCGGAGCGGGGGGTTTTAATGTGTGCTGCTTGGACCAGCGGGGTTTTTTTGGGtg                                                                                                                                                                                                    >  2:607086/1‑238 (MQ=255)
                                    tatgcgggtgggtcGTTTTGTTGGCCCGACGCGTCCTCTGGTGCCAATGGTGGCGGGAATGCGGGATCTGCCGGTGGGTAAATTTATTCCGCCAAATATTATCGGGTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTtggtg                                                                                                                                                                                                <  1:209489/233‑1 (MQ=255)
                                         tGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGGATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTTCCGGGATGCGAAGCGGTGGGGTTTAATGGGTTCTGCTGGCCACAGCGGGGGTTTTGTGGGtgtgggggggg                                                                                                                                                                                           >  2:426289/1‑229 (MQ=255)
                                         tGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGGGGGGTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTTGGGGGTTGgtgggtgg                                                                                                                                                                                           >  1:399440/1‑239 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGCATGCTGGATCTGCCGGTGGCTTACTTTATTAAGCCGAATATTATCGGCTGCCTGCCGTGGGCGCCGTTTTACTTTCTGCCCGGGGTTCTGGCGGGCGCGGCGGGCGGTGTTCCTGCCGGAATCCGGGAGGGGGGGATTTAACTGGTTGTGCTGGCAAACACGGGGGTTTTGTGGGGtgggggggggtg                                                                                                                                                                                         >  2:789454/1‑233 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGGGGGGGGGTCCGTATTTCTGCCGGGATGCAGAGTGGGGGCGTTAAATGGTTTTTGTTGGGAAAACCGGGGTTTTTtgggggtggggggggggg                                                                                                                                                                                         >  2:882508/1‑233 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTGCCGGAATGCAGAGCGGTGGGTTTAAATGGTTGCTGCTGGCACCACCGGTGTTTTTGTGGGGTGGtggcgg                                                                                                                                                                                            >  1:639977/1‑234 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCGGCTGGCAACAGCGGTGGTTTTGTGGGTTGGtggcgg                                                                                                                                                                                            >  1:284905/1‑234 (MQ=255)
                                                tcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAAAATTATCGGCGGCCTGCTGTGGCCGCCGCTTTACTTCCTGCCCGGGATTCTGGCGGGCGCGGCGCTCGATATTCCTGCCGGGATGCGGAGGGGTGAGGTTATAATGGGGTGGCTGGGAACAGCGGGGGTTTTTGGGGGTgggggtggggatg                                                                                                                                                                                        >  2:796909/1‑226 (MQ=255)
                                                 tgTTTTGTTGGCCCGGCGGTTCCGCTGGGGCCAAGGGTGGGGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTACCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  1:848409/237‑1 (MQ=255)
                                                 ctttttGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCGGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  1:84490/236‑1 (MQ=255)
                                                 cgTTTTGTTGGCCCGACGGGTCCGCTGGTGCCAATGGGGGCGGGAATGGTGGATGTGCCGGTGGATAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  1:706282/238‑1 (MQ=255)
                                                                aCGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAAAGGTTGCTGCTGGCAACAGCGGGGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCggggtttgtgccg                                                                                                                                                                    >  1:161199/1‑228 (MQ=255)
                                                                            gTGCCAATGGTGGGGGGAATGCTGGATTGGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                                                                                         <  1:609216/238‑1 (MQ=255)
                                                                              gCCAATGGTGGGGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTCCGCCGAATATTAGCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACt                                                                                                                                                       <  1:204918/238‑1 (MQ=255)
                                                                                      tggGGGAAATGGTGGTTCTGCCGGGTGTAAATTTTTTTCCCGCGAATTTTGTGGGCGGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGttt                                                                                                                                               <  2:161199/238‑1 (MQ=255)
                                                                                           ggAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATTTTAGCGGCTGCCTGCGGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                                                                                          <  1:426289/238‑1 (MQ=255)
                                                                                                         gCCGGTGGCTAAATTTATTACGCCGAATATTACCGGCTGCCGGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCcgcg                                                                                                                           <  1:423867/239‑1 (MQ=255)
                                                                                                               ggCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGt                                                                                                                      <  1:723286/238‑1 (MQ=255)
                                                                                                                           taCGCCGATTATTAGCGGCTGCCTGGTGTGGCCGACGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGa                                                                                                         <  1:207293/239‑1 (MQ=255)
                                                                                                                                                 cctgcTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCAGCGGGGAAGAGACCGACCGCTTTGGTCCTTTTTTTTTCCCCGGGCGGTTTTTTTGGGTGAGCCCCGTTGtttttctcctcc                                                                                    >  1:7721/1‑231 (MQ=255)
                                                                                                                                                      tgtgGCCGCCTTTTTCCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtg                                                                              <  1:819452/239‑1 (MQ=255)
                                                                                                                                                         ggCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggt                                                                            <  1:260966/238‑1 (MQ=255)
                                                                                                                                                             gccgTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgcgc                                                                       <  1:789454/239‑1 (MQ=255)
                                                                                                                                                                                 ggtttCTGGCGGGGGGGGGGATCGATTTTCCTGCCGGAAGTCAGAGGGTTGGGTTTAAATGGTTGGTTCTGGCAACAGCGGTGTTTTGGTGGGTTGGTGGCTGGCTTGGCTGGGGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCa                                                    <  2:656616/235‑1 (MQ=255)
                                                                                                                                                                                           gggcgCGGGGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                          <  1:607086/238‑1 (MQ=255)
                                                                                                                                                                                             ggccgggcgTTGGATTTTCCGGCCGGAATGCAGGGGGGTTTGTTTAAATGGTTGCTGGTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGc                                         <  2:399440/233‑1 (MQ=255)
                                                                                                                                                                                                     gatcgatATTCCTTCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTTGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtat                                 <  1:500501/238‑1 (MQ=255)
                                                                                                                                                                                                             ttCCGGCCGGAATGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtt                        <  1:436346/239‑1 (MQ=255)
                                                                                                                                                                                                                            gaggggggTGGGTTTAAATGGTTGCTTGGGGGAACAGGGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATCCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  2:284905/233‑1 (MQ=255)
                                                                                                                                                                                                                            gagagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  1:104492/238‑1 (MQ=255)
                                                                                                                                                                                                                             agggcgggtaGTTTAAAGGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  1:737373/229‑1 (MQ=255)
                                                                                                                                                                                                                             agagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  1:882508/238‑1 (MQ=255)
                                                                                                                                                                                                                               atcGGTGAGTTTAAAGGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg        <  1:508028/236‑1 (MQ=255)
                                                                                                                                                                                                                                    tGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGtt   <  1:779347/238‑1 (MQ=255)
                                                                                                                                                                                                                                    tGAGTTGAAATGGTTGCTGGTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGtt   <  1:357728/238‑1 (MQ=255)
                                                                                                                                                                                                                                     gAGTTTAAATTGTTGCTGCTGGCAACAGCTGTGTTTTTGTGGGTTGGTCGCTGGCTGTGCGTGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGttt  <  1:404765/238‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
AACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTT  >  NC_000913/71650‑72113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: