| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 75,091 | G→T | 37.4% | D130E (GAC→GAA) | thiB ← | thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 75,091 | 0 | G | T | 37.4% | 25.8 / 3.4 | 24 | D130E (GAC→GAA) | thiB | thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (3/12); new base T (7/2); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTT > NC_000913/74875‑75329 | gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGTC‑GGGGTTTTACGTTTTTTTTTTTCTT‑TAACGaaatgcaaggag > 1:751377/1‑228 (MQ=255)gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTGTTAT‑AAAAAAAgggaaagaag > 2:244461/1‑231 (MQ=255)gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAGTTTTTTCTTTTTCT‑TAACAAAAGCGCAGTAg > 1:68849/1‑238 (MQ=255) cTTCCCCCCAGTTTTTGTTGCCCGTGCCCGTTTTTTCCGCCATTTTCGCCCGGGTTGGGGGGGCGTCTTCGCCATAGATTTTTTGCACCAATAGCAACAGCCCCAGCCCGGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCa < 2:689352/239‑1 (MQ=255) ttCGCTCCAGCTTTGGGTTACTGGCACCGTTTTCGTCGCCAGTTGCTGCCGGGCTGGGGGGGCGTCATGACCAAAGACTTTTTGCATCCATAGCAACAGCCCCATCCCCGGTTTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCa < 1:62529/238‑1 (MQ=255) tCGCTCCCGCCTTGGGGCACGTTGCCCTTTTTCTTCGCCAGTTGCTGCAGGGCTTGGGGGGCGTCATCGCCTTAGACTTTTTGCACCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCaaa < 1:377130/239‑1 (MQ=255) tCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGCC‑GGGGTTTTCCTTTTTTTATTTTTAT‑AAACAAAAGCGAAGTGGtcatcatcaca > 1:321225/1‑237 (MQ=255) ggTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGCGTTTTTAGTTTTTTTTTTTCAT‑ATACAAAAAGGCAGTAGTCAATATTACACTGTACGaaacggg > 1:20953/1‑235 (MQ=255) ccgtgaccgtTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTCAGTTTGTTTTTTTCTT‑AAAACAAGGCGGAGTAGCCCTAATCAACAGGGAAGAAAAAGTCatta > 1:823010/1‑239 (MQ=255) ggCGTCAGCGCCATAGCCTTTTGGCATCCATAGCAACAGCCCCAGCCCCGGTGTACGGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCcaca < 1:244461/238‑1 (MQ=255) tAGCAACAGCCCCGGCCCGGGTGTACTGGTGCGCGGATCCTGATAAACCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTg‑g < 1:743231/239‑1 (MQ=255) acacgACCCAGCCCGGGTGTATGGGTGCCCGGATCCTGATAAACCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTg‑gcggc < 1:264522/235‑1 (MQ=255) aCCCAGCCCCGGTGTACTGGTGCTCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGATTTTTCAGTTTGTTCTTTTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCCGGAAAGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑GCAACAGTCCGGTTTTACTT‑GAGGCGACTa > 1:697796/1‑239 (MQ=255) gCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTCAGTTTTTTTTTGTCTT‑AAACGAAGGCGAAGTAGCCCTAATCAAACGGTACGAAAATGTCATTATTCCCG‑CCGCCGGGGACGCTAACGGCATCCCCCGCCACACCCCTTTTTG‑CCAAAAACCCGGCTTTATTG‑GCGGCGGCTTACAgg > 1:571791/1‑239 (MQ=255) gggTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAg < 1:657677/206‑1 (MQ=255) ggtgggggATTCTTATAAATCCCCCGCAAGTTTTGATCGCTCCCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGccc < 1:233420/233‑1 (MQ=255) cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAGTTTTTTTTTTTCCTAAAACAAAGGGGAGGAGCCCATAATCAAACGTGAGGCAAG‑GTCATTTTTTCAGACCCCCGGGAAGGTAAACGCCA‑CCGCCTCCACCCCCCCTTTGGTCAAAAAGCCGGGTTTTATTGTGCCGCG‑CTAAAAGATTGTTATACAcccaa > 2:231752/1‑236 (MQ=255) cttaTAACTCCCCCTCCATTTTTGACCGCTCTCAACCAGTTTTTTCAGGCTTGGTGGC‑GGTTTTTTCAGTTTGTTTTTGTCAT‑AAACGAAGGCGAAGTAGCCAAAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt < 2:20953/236‑1 (MQ=255) tGATCGCTCTCACCCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCAAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTc < 1:439629/239‑1 (MQ=255) tttgGG‑GGTTTTTTCTGTTTTTTTTTTTCAT‑AACCAAAGGGGAAGTAGCAATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGcc < 2:823010/237‑1 (MQ=255) tttttCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAATTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCa < 1:574853/238‑1 (MQ=255) ttCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACCGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCCTCCGTCGACGGTTGATAAGCGCAAAGCCATCTTCCAgcg > 2:753735/1‑238 (MQ=255) tCAGTTTGTTCTGTTAAA‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTTTTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGcc < 1:231752/239‑1 (MQ=255) tttttGTCAT‑AAAGTAAGGCAAGTTAGGTATAATCAAACGGTACGAAATTTGCATTATCCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGttt < 2:279749/236‑1 (MQ=255) gTCAT‑GAACGAAGGCGAAGTGGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGtt < 1:594890/239‑1 (MQ=255) | GCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCAT‑AAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAG‑CCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGG‑CAAACAGTCCGGTTTTACTG‑GCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTT > NC_000913/74875‑75329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |