Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,154,491 T→C 21.9% L159P (CTC→CCC)  mdtA → multidrug efflux system, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,154,4910TC21.9% 102.4 / 4.6 62L159P (CTC→CCC) mdtAmultidrug efflux system, subunit A
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base C (8/7);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
aTTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGttt                                                                                                                                                                                                                                     >  1:330767/1‑238 (MQ=255)
         gcTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTACCCAAAGCCCAAGGCCACCTGGCACAAGATAAAGCCACGCTTGACAACGCCCGCCGGGACCTGGCGCCGTATCAAACACCGGCAAAAACCAATCCCGTTTCCCGCCCGGa                                                                                                                                                                                                                           >  2:237821/1‑239 (MQ=255)
                   ttACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCCCGCTTGCCCACGCCCGCCGTGACCTGGCGCGTTATCAACAAATGGCAAAAACCACACCCGTTTCCCGCCGGGAGCTGGATGc                                                                                                                                                                                                                  >  2:689588/1‑238 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTTTCAACAACTGGCAAAAACCAATACCGTTTCCCCCCAGGAGCTGGATGCCCAAAAGgc                                                                                                                                                                                                         >  2:292347/1‑239 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGgc                                                                                                                                                                                                         >  1:314236/1‑239 (MQ=255)
                             cAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCAAAAGCCCAGGGCCAACGGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGCCCTGGCGCGTTATGAACACACGGAACAACACAACCCCGGTCCCCGCCAGGAGCCGGAGGCCCAACaagggc                                                                                                                                                                                                       >  2:563080/1‑234 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACACCTGGCAAAAACCAACCCCCTTTCCCGCCCGGGGCGGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  2:716453/1‑239 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  1:690556/1‑239 (MQ=255)
                                     tGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGTAGCCCAACAGGCGCTGGTCAGt                                                                                                                                                                                               >  1:247081/1‑239 (MQ=255)
                                                      gCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAACCCACGCTTGCCAACGCCCACCGTGCCCTGGCGCGTTATCAACAACTTGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACACGCGCTGGACAGAGAACCCGAAGGCAcc                                                                                                                                                                               >  1:159241/1‑237 (MQ=255)
                                                          tACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCATTGAAACCGAAGGCAACATTAAg                                                                                                                                                                         >  1:184469/1‑239 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAg                                                                                                                                                                         >  1:863597/1‑238 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCaa                                                                                                                                                                                                              >  1:757148/1‑202 (MQ=255)
                                                                 ccgagAAGGCCAGAGGGACAAAGCAGGCGTTTTACTGGCAAAAATTGCCCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCAAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCtgatg                                                                                                                                                                  <  1:226057/235‑1 (MQ=255)
                                                                 ccAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCCAAAACCAATCTCGTTTCCCGCCAGGAACTGGATGCCCAACAGCCGCTGGGCAGTGAACCCGAAGGCACCCTTTATGCtgaag                                                                                                                                                                  >  1:801966/1‑237 (MQ=255)
                                                                      aaGGCCGGCAGGTCAAGGCAGGCGTTTACCGGGAAAAATTGGACCCCAGCCAGTCCAAAGTTCAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAAAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaag                                                                                                                                                               <  1:428537/237‑1 (MQ=255)
                                                                       gggCCAGGAGGTAAAAGCAGTCGTTTTACGGAGAGAATGGCACCCACCCCTGTTCAAAGTTGCATTAGCCAAACCCCGGGGCCACCGGGCAAAAGAAAAACCCACGTTTGCCACCGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  2:516880/237‑1 (MQ=255)
                                                                       aGGCCAGCAGGTCAAAGCGGGGGATTACCGGGCAAAAATTGCCCCCAGCCAGTTAAAAGTTGAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAAAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  1:716453/238‑1 (MQ=255)
                                                                        ggCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCCCTGGTCAGTGAAACCGAAGGCCCCATTAAAGCTGATGaagaaag                                                                                                                                                           >  2:193844/1‑239 (MQ=255)
                                                                                   cAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAATCCACGCTTGCCAACGCCCGCCGTCACCTGGCGCGTTATCAACCACTGGCAAAAACCAATCTCGGTTCCCGCCAGTGGCCGGATGCCCACAAGCCCCTGGCCCGGGAAACACCAGACACCATTAAGGACAAAGaagcaagagattcaaagg                                                                                                                                                >  2:388806/1‑228 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGCAGGAACCATTAAGGCTGATGAAGCAACCGTTTCCTGCGCGCAGcct                                                                                                                                        >  2:158874/1‑237 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCCGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCCAAGGCCCACTTAGGGCTGCTGAAGCCACCGCTGCCAGGCCGCAGCt                                                                                                                                         >  2:55720/1‑238 (MQ=255)
                                                                                                                        cacgTTGAAATTTGCATTGGCAAAAGCCAGGGGCCAACGGGAAAAAGATAAACCCAAGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCAc                                                                                                            <  1:767962/236‑1 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACa                                                                                                                                                                                                            >  1:330064/1‑130 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGCCTGGAGCCGGATTACCGCACCAGCCGAGGGTcgc                                                                                              >  1:391665/1‑239 (MQ=255)
                                                                                                                                        ttACCACCACCCCGGGCCCATCTGGAAAAAAAAAACCCCACCTTTGCCAACCCCCGCCGTGCCCTGGCGCGTTACCAACAACGGGCAAAACCCAATCTCGGTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGt                                                                                            <  2:242013/239‑1 (MQ=255)
                                                                                                                                           gCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGACGAAGCAAGCGTTGCCAGCCCGAAGCTCCAACTCGACCGGAGCCGGCTTACCGCACCAGCCCATggctgccttg                                                                                          >  1:425021/1‑230 (MQ=255)
                                                                                                                                             ccaACCCCCGGGCCCATGGGGAAAAGATAAAACCCCCGCTTCCCACCCCCCGCCGTGACCGGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGt                                                                                        <  2:690556/238‑1 (MQ=255)
                                                                                                                                                aGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGACGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGGCGCACCCCAACGGGAGCCGGATTACCGCCCCAGCCGATGGTCGCGTTgggccaa                                                                                    >  2:499818/1‑234 (MQ=255)
                                                                                                                                                   ccAGGCCAAACTGGAAAAAAAAAAAGCCACGGTTGCCAAGCCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAg                                                                                  <  1:700888/238‑1 (MQ=255)
                                                                                                                                                      gggCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAgg                                                                              >  1:169768/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGTGCTCAAGCAGGTTGATg                                                                        >  1:752410/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATg                                                                        >  1:231589/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCAGCAACTCGACTGGAGCCGGATAACCGCACCAGCCGATGGTCGCGTTGGGCTCAAGCAGGTTGATg                                                                        >  1:880907/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGCTGCCAGCGCGCCGCTGCAACCCGACTGGGGCCGGGTTACCGCCCCAGGCGATGGGCCCGTGGGGCTCACGCCGGTTGagt                                                                        >  2:66664/1‑237 (MQ=255)
                                                                                                                                                                        taaaaCCCACTTTTGCAAACGCCCGCCGTGACCTGGCGCTTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  1:657492/237‑1 (MQ=255)
                                                                                                                                                                           aaGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAGTCAGGTTGATGTTGGTAACCAAAt                                                           >  1:252960/1‑238 (MQ=255)
                                                                                                                                                                                  gCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGACGGTGGTAACCAAAACTCCAatg                                                   >  1:185925/1‑239 (MQ=255)
                                                                                                                                                                                      gCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGACCCGGATTACCGCACCAGTCGACGGCCGCGTCGGTCCCAAGCAGGTTGATGTTGGTAACCAAATCACCAGGGGGGa                                               >  2:700823/1‑239 (MQ=255)
                                                                                                                                                                                       ccactgcccgccGTGACCTTGGGGTTTAACAAAAACCGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCTCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGAt                                              <  2:180080/234‑1 (MQ=255)
                                                                                                                                                                                        cAACGCCCGCCGGGACCTGGCGGGTTAGCAACAACGGGAAAAAACCAATCGCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATa                                             <  1:827476/239‑1 (MQ=255)
                                                                                                                                                                                         cccccccgccGTGTCCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATac                                            <  2:391665/235‑1 (MQ=255)
                                                                                                                                                                                             ccccccTTGCCCTGGCGCTTTTTTAACAACTGGAAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGACCAACAGGCGCTGGTCAGTGAAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATaccac                                         <  2:330767/239‑1 (MQ=255)
                                                                                                                                                                                                    tgcccTGGCGCGTTATCAACAACCGGCAAAAACCATTCTCGTTTCCCGACAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCg                                 <  1:402075/236‑1 (MQ=255)
                                                                                                                                                                                                     gcccTGGAGCTTTATCCACAACTGGCAAAAACCAAACTCGTTGCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGAAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  1:699507/237‑1 (MQ=255)
                                                                                                                                                                                                      aCCGGGCGCGTTAACAAAAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  1:699506/238‑1 (MQ=255)
                                                                                                                                                                                                        cgggCGCGGTATAAACAACTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                             <  1:143650/237‑1 (MQ=255)
                                                                                                                                                                                                         tGGGGCGTTACCAACACCTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  1:182002/239‑1 (MQ=255)
                                                                                                                                                                                                         tGGCGGGTTACCAACAACTGGCAAAAGCCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  1:138913/239‑1 (MQ=255)
                                                                                                                                                                                                          ggggcgTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                           <  1:22090/236‑1 (MQ=255)
                                                                                                                                                                                                          ggCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAACCGTTGCCAGCGCGCAGCTGCACCTCGCCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCCCAAGCAGGTTGATGTTGGTAACCAAAACTCAAGTGGTGATAAAACCGGGATCggggtgc                           >  2:574553/1‑238 (MQ=255)
                                                                                                                                                                                                            cgcgTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                          <  1:66664/238‑1 (MQ=255)
                                                                                                                                                                                                               gTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  1:168757/239‑1 (MQ=255)
                                                                                                                                                                                                                ttaacaaacacTGGAAAAACCCAAGCTCTTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  2:247081/229‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGGAAAAACCAATCCGGTTTCCCGCCAGGAGATGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCATCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATTGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCTcc                      <  2:159241/238‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGTTCGTGGGGATCAccc                     >  1:207520/1‑239 (MQ=255)
                                                                                                                                                                                                                         acgtgaaaaaaCCAATCTCTTTTCCCCCCAGGGGCTGGATGCCCAACAGGGGCTGGTCGGGGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATc            <  2:8451/233‑1 (MQ=255)
                                                                                                                                                                                                                            ggCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACGGGCGCTGGTCAGTGAAACCGAAGGCCCCCTTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa         <  1:121728/239‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAACCCTATCCCGTTTCCCGCCAGGGCGTGGACGCCACACAGGCGCTGGTTAGTGAAACCGGAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  2:131415/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  1:689588/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  1:578393/238‑1 (MQ=255)
                                                                                                                                                                                                                                   aCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt  <  1:574588/239‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: