Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,507,677 T→C 13.5% E73G (GAG→GGG)  fryC ← putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,507,6770TC13.5% 174.0 / 2.5 72E73G (GAG→GGG) fryCputative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base C (5/6);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCGA  >  NC_000913/2507444‑2507912
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTCTTAAGAGCATCCATGAAGCCAATCAcacca                                                                                                                                                                                                                                        >  2:261909/1‑239 (MQ=255)
tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGACCTcagca                                                                                                                                                                                                                                        >  1:211146/1‑239 (MQ=255)
  cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGCAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGAGGCCGATCTcacaagg                                                                                                                                                                                                                                      >  1:299335/1‑234 (MQ=255)
   tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATACCGAGCTCAGCAGGa                                                                                                                                                                                                                                     >  2:23418/1‑239 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGGCTGCGACAGCCAGGCAGATTTAAGTAAGGAGGGGTCGAAACCGGAGACTTTCCCGCTATTAAAGGCATCCCTGATGCCGACACCAGCAGGa                                                                                                                                                                                                                                     >  1:492924/1‑237 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGGGAGGTCGAAACCGGGAAATTTCCCGCTATTAAGAGCATCCATGAGGCCGAACTCAGCAGGAAt                                                                                                                                                                                                                                   >  2:219870/1‑239 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGAAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAACTTAAGTAAGGAAAGGTGGAAACCGACGAATTTCCCGCTATTAAGATCATCCATGATGCCGATCCCAGACGGAAt                                                                                                                                                                                                                                   >  1:162969/1‑239 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAGACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCCCCATTAAGAGCATCCATGATGCCGATCCCGGCAGGAATTTTa                                                                                                                                                                                                                               >  1:162027/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACACAACGCCCCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGACAGGCCGAAAACGGCAAATTTCCCGCTCTTAAAAACCACCAAGATGGCGATCCCCCCAAGAATTTTa                                                                                                                                                                                                                               >  2:317340/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCTCCGAAGGGTTGCGACAGCCAGGCAAATTTAAGTACGGAGAGCGCGAAACCCGAGCATTTCCCGCTATTAAAAGCCTCCCTGCTTCCGCTCTCAGCAGGGAttttt                                                                                                                                                                                                                               >  2:67676/1‑237 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTa                                                                                                                                                                                                                               >  1:715285/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCCCAGCAGGAATTTTa                                                                                                                                                                                                                               >  1:681009/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAAAGCATCCATGATTCCGATCTAAGCAGGAATTTTaa                                                                                                                                                                                                                              >  1:823414/1‑239 (MQ=255)
                           tCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCAATAATGCCGATCTCAGCAGGAATTTTAAGCCAACAATaaaaaaaa                                                                                                                                                                                                             >  2:585500/1‑233 (MQ=255)
                                tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAAAAACACGCCGCCGAAGGACTGCGACAGCCCGGCAAATTTAAGTAAGGAGAGGACGCAACCGGAGAATTTACCGCTATTAAGAGCATCCCTGATGCCGATCTCAGCAGGGATTTTAAGCCCGCAATAAGCAAACAgctg                                                                                                                                                                                                        >  2:528265/1‑239 (MQ=255)
                                       cccccccccccccGAAAAACCCAGCCGGGAACCCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGaaa                                                                                                                                                                                                 <  2:756020/232‑1 (MQ=255)
                                         cacccccccccGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  2:211146/234‑1 (MQ=255)
                                         accAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  1:105935/237‑1 (MQ=255)
                                         aaccccccGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  1:351618/234‑1 (MQ=255)
                                         aTCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               >  1:53835/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGGTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCACCTGGCTGAAAACGAg                                                                                                                                                                                            >  1:881497/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAg                                                                                                                                                                                            >  1:302706/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTAAAAGCAATAAACGGGCTGAAAGCGAg                                                                                                                                                                                            >  2:278969/1‑239 (MQ=255)
                                             ccccGCCGAAAAAACCAGCCGGGACCGCCAGTTTGCCCCCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAGACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  2:162027/234‑1 (MQ=255)
                                                   caaaaaacCCAGCCGGGAACCCCCGTTTGCCCCCGAAGGAGTTCGCCCCAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATcaca                                                                                                                                                                                     <  2:823414/235‑1 (MQ=255)
                                                        aaaCCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGcc                                                                                                                                                                                <  1:627704/239‑1 (MQ=255)
                                                             aGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATaa                                                                                                                                                                           >  1:296681/1‑239 (MQ=255)
                                                               ccctgAACCCCAGTTTGCCACGGATGAGGTTCGCCACAAAACCAGAACACGTCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACCGGCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTCCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACCCCGCCCATAAtt                                                                                                                                                                         <  2:753031/235‑1 (MQ=255)
                                                               cccggAACCCCAGTTTGCCACCGATAGAGTTCGCCCCAAAAGCAGCAAACAGCGGAATGGAAAAACCAAACAACCCGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         <  2:53835/236‑1 (MQ=255)
                                                               ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         >  1:39570/1‑239 (MQ=255)
                                                               ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         <  1:219870/239‑1 (MQ=255)
                                                                 ggaaccGCCCGTTTGCCACCGATAAAGGTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGGAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTaa                                                                                                                                                                       <  2:704998/234‑1 (MQ=255)
                                                                         cAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGACGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCAAGCTATAAGAAATCAGCTGGCTGAAAGCAAGGATCCAACCCACACTAATTAAGGTCGGc                                                                                                                                                                >  2:383444/1‑238 (MQ=255)
                                                                                ccACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACCCGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCg                                                                                                                                                        <  1:334649/239‑1 (MQ=255)
                                                                                  cccGACAGAGTTGCCACCAAAAGCACCAAACATCTGAATGGGAAAACCAAACAACACGCCGTCGAAGGGATGCGACAGCGAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTg                                                                                                                                                      <  2:259306/238‑1 (MQ=255)
                                                                                             tCGCCAAAAAAGCAGCAAACAGCGGAATGGAAAAACCAAACAACCGGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAATAAGAGAATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGAc                                                                                                                                           <  1:67676/239‑1 (MQ=255)
                                                                                              cGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGCTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCCCCCCTAATTAAGGTCGGCACCATGCGTGAAATCCCTGAc                                                                                                                                           >  2:474302/1‑238 (MQ=255)
                                                                                                    aaaaaGGAGAAAACAACGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGAGGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATcaca                                                                                                                                     <  2:539261/238‑1 (MQ=255)
                                                                                                            gcaAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATTCGTGAAATCCCTGACATCACATGCTCCgg                                                                                                                             >  1:594057/1‑239 (MQ=255)
                                                                                                              aaaCATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACAtgctgc                                                                                                                               <  1:261909/235‑1 (MQ=255)
                                                                                                                 cATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGtt                                                                                                                        <  1:420273/239‑1 (MQ=255)
                                                                                                                                         cacGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTagag                                                                                                 >  1:117510/1‑238 (MQ=255)
                                                                                                                                              cggcgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCTGCTATTAAGAGCAACCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAAGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGc                                                                                           <  1:838112/236‑1 (MQ=255)
                                                                                                                                               gccgAAGGGCTGCGACAGCCAGGCAAATTTAAGTAAGGATAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCg                                                                                          <  1:309811/239‑1 (MQ=255)
                                                                                                                                                ccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCAAGGATAACACCGCCCATAATTAAGATCGGCACCCTCCGGGAAACCCCTGACATCACATGCTGCGGGAGTTCCCCCCAGAACCTGGTTTTAGAGGGCTgggg                                                                                         >  2:233367/1‑239 (MQ=255)
                                                                                                                                                 cgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGGCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGa                                                                                        <  1:11115/239‑1 (MQ=255)
                                                                                                                                                           cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAAct                                                                               >  1:713459/1‑238 (MQ=255)
                                                                                                                                                           cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAAct                                                                               >  1:54727/1‑238 (MQ=255)
                                                                                                                                                               cgCCAGGTCAATTGAAGTAAGGAGAAGTCCAAACCGGAAAATTTCCCCTTTTTAAGGACAACCATTATGCCGGTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCCGCTGGCTGAAAGCAGGGATCACACCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAActgccg                                                                           <  2:57352/237‑1 (MQ=255)
                                                                                                                                                                gCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGCCATCACATGCTCCGGGCGTTCAACCCAGAAGCTGGTTTTAGAGGCCTGCGGGTTCTTAActgcg                                                                            >  2:709838/1‑235 (MQ=255)
                                                                                                                                                                               gtaagGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGACCTGCGGATTCTTAACTGCCGCTCCCGCACCgggagc                                                           >  2:447138/1‑234 (MQ=255)
                                                                                                                                                                                 aagggagggTCAAAACCGGAAAATTTCCCCCTATTAAGGGCATCCATGATGCCGATCCCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGcc                                                        <  2:715285/232‑1 (MQ=255)
                                                                                                                                                                                 aagGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGcc                                                        <  1:54248/239‑1 (MQ=255)
                                                                                                                                                                                        ggTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  1:278969/238‑1 (MQ=255)
                                                                                                                                                                                        ggTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCAGGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  1:84930/238‑1 (MQ=255)
                                                                                                                                                                                        agTCGAAACCGGAGAATTTCCCGCTATTAAGAGCAGCCATGAGGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  1:195116/237‑1 (MQ=255)
                                                                                                                                                                                         gTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACGGCCGCTGCCGCACCGGATCCGCCGGGCCCaa                                                >  1:680075/1‑239 (MQ=255)
                                                                                                                                                                                                 cGGAGAATTTCCCGGTATTAAGAGGATCCATGATGCCGATCTCAGCAGGAATTTGAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCa                                        <  1:632487/239‑1 (MQ=255)
                                                                                                                                                                                                   gagaATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCCCCCCAGAACATGGGTTTAGAGGCCTGCGGGTTCTTAACTGCCCCTGCCGCACCGGATGCGCCAGGCACAACGGGTGCAca                                      >  2:801656/1‑238 (MQ=255)
                                                                                                                                                                                                    agaATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACt                                      <  1:23418/238‑1 (MQ=255)
                                                                                                                                                                                                             cgttATTAAGAGCATCCATGAGGCCGATCCCAGGAGGAATTTTAAGCCAGCTAAAAGCAATCAGCTGGGTGAAAGCGAGGATCACACCGCACATAATTAAGGTCGGCCCCAGGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGGTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTa                            <  2:302706/236‑1 (MQ=255)
                                                                                                                                                                                                                     agagacaccATGATGCCGATCCCAGCAGGAATTTTAAGCCAGCTAAAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCAt                    <  1:596677/232‑1 (MQ=255)
                                                                                                                                                                                                                     agagCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCAt                    <  1:624845/239‑1 (MQ=255)
                                                                                                                                                                                                                        gcctCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATa                   <  1:183164/234‑1 (MQ=255)
                                                                                                                                                                                                                          aTCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCCCCAGGCACAACGGTTGCCCTGCGTTTTTCAATGGCCATAAcca               >  1:456433/1‑237 (MQ=255)
                                                                                                                                                                                                                          aTCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCCCCAGGCACAACGGTTGAACTGCGTTTTTTCATGGCCATAaaaa               >  1:379435/1‑239 (MQ=255)
                                                                                                                                                                                                                            ccATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGGTGCGCCAGGCACAACGGTTGCACTGGGTTTTTTAATGGCCATAaaaaaa             >  2:387222/1‑237 (MQ=255)
                                                                                                                                                                                                                                gtcgCCGAGCCCAGGAGGAATTTTAAGCACACTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGTCCATAATTAAGGTCGGCACCAGGCGTGAAACCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATcc         <  2:681009/236‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCCATTTCAGCAGGAATTTTAAACCAGTTTTAAGCAAACAGCGGGCCGAAAGCGAGGATCACCCCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTg      <  2:296681/239‑1 (MQ=255)
                                                                                                                                                                                                                                     cGATCCAAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTg      <  1:796467/237‑1 (MQ=255)
                                                                                                                                                                                                                                      gATCCCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCCCCCCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCg   <  1:795627/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCCGAAGCTGGTTTTACAGGCCTTCGGATTCTTAACTGCCCCTGCCGCCACGGATGCCCCCGGCCCCACGGTTGGCATGCGCTTTTTCAATGCCATCACAAATATCCCAGTcca  >  2:411647/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCGA  >  NC_000913/2507444‑2507912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: