Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,265,360 T→C 17.2% G223G (GGA→GGG tdcB ← L‑threonine dehydratase, catabolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,265,3600TC17.2% 155.3 / 2.5 70G223G (GGA→GGGtdcBL‑threonine dehydratase, catabolic
Reads supporting (aligned to +/- strand):  ref base T (43/12);  new base C (4/10);  total (48/22)
Fisher's exact test for biased strand distribution p-value = 8.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT  >  NC_000913/3265125‑3265595
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
gAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                                                                                                                                                          <  1:753453/239‑1 (MQ=255)
 aaTATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGTTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGGTATTTCCCCga                                                                                                                                                                                                                                         >  2:555507/1‑238 (MQ=255)
   tatatTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAg                                                                                                                                                                                                                                       <  1:466326/239‑1 (MQ=255)
      atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGCCCAATTCACTAACGATTTCGTAAGTTAAATTACCCGGGCGGGCGCCATCCCAACCATCCGCCAGGGGGCCGGTAGTACGGGGCGTGGTTATTTCTCCGGAgggg                                                                                                                                                                                                                                    >  2:421188/1‑239 (MQ=255)
      atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTgg                                                                                                                                                                                                                                    >  1:455260/1‑239 (MQ=255)
      atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGGGCGTGGTTATTTCTCCGGAgggg                                                                                                                                                                                                                                    >  1:341152/1‑239 (MQ=255)
        tGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGGGTGGaa                                                                                                                                                                                                                                  >  1:709339/1‑239 (MQ=255)
           tttattttCCCGTTTAAAAAGGCGGCCCAGGCCAGGCGCCCGGGCCTTTCGGTGACGACTTTATTGCGCGGAATTAAGGCAAACATTCTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAaaga                                                                                                                                                                                                                               <  2:433810/234‑1 (MQ=255)
           tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCCAGAACGATTTCTTAAGTTACATTACCCGGGCGGGAGCAATCACAACCAACCGCCAGGGGGCCGTAAGTTCGGGCGGGGGTATTTTCTCCGGAGTGGaaaaa                                                                                                                                                                                                                               >  2:829867/1‑237 (MQ=255)
           tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTCCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCCCAACCATCCGCCAGGGTGCCGGTAGTTAGGTGCGTGGTTATTTTTCCGGAGTGGAaaga                                                                                                                                                                                                                               >  1:402298/1‑239 (MQ=255)
                    ccGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGGGCTGGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCAt                                                                                                                                                                                                                      >  2:610376/1‑239 (MQ=255)
                     cGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGCTGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTAGGTGCGTGGTTATTTCTCCGGAGTGGAGAGAAGCCGCCAt                                                                                                                                                                                                                      >  1:522973/1‑238 (MQ=255)
                     cGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCCCAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGGGTGGAAAGAAGCCGCCAt                                                                                                                                                                                                                      >  1:349611/1‑238 (MQ=255)
                       cTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGCGCCATCACAACCATCCGCCAGGGGGCCGTTAGTCCGGACCGAGGGTATTTATCCGCCGTGGCAAGAGGCCGCCATGcc                                                                                                                                                                                                                   >  1:474228/1‑239 (MQ=255)
                          aataatGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCCCAACCATCCCCCAGGGTGACGGTAGTTCGGTGCGTGGTTATTTCACCGGAGTGGAAGCAAACCGCAAAGCCggg                                                                                                                                                                                                                >  2:40498/1‑237 (MQ=255)
                          aataatGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGGGCCGGTAGTTCGGGGCGTGGTATTTTTCCCGGGGGGGAAAAAAGCCGCCATGcgcgc                                                                                                                                                                                                                >  2:275416/1‑235 (MQ=255)
                               tGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGATTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCTTAAGTTAAATTACCCGGGCGGGAGCCATCCCAACCATCCTCCAGGGTGCCGGTAGTTCGGGGCTTGGTTATTTTTCCGGAGGGGAAAGAAGCAGCCTTGCCGGGAACGt                                                                                                                                                                                                           >  1:77570/1‑239 (MQ=255)
                                  agcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGCGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCTGGAACGtttt                                                                                                                                                                                                        >  2:197663/1‑239 (MQ=255)
                                  agcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACACGGGCGGGAGACATCACAACCATCCGCCAGGGGGCCGGTAGTTCGGGGCGTGGTTATTTATCCGGGGTGGAAAGAACCCGCCCTGCCGTGGACGttt                                                                                                                                                                                                         >  2:13755/1‑238 (MQ=255)
                                    cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCACTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGGGCGTGGTTCTTTCTCCGGGGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                                                                                                                                                      >  1:654462/1‑239 (MQ=255)
                                    cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTTACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGCGCCGGTAGTTCGGCGCGTGGTTATTTCTCCGGTTTGGAAAGAAGCCGCCATGCCGTGAACGTTTTc                                                                                                                                                                                                       >  1:767042/1‑238 (MQ=255)
                                    cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGCGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGAGGGTGGTTATTTTTCCGGAGTGGAAAGAAACCGCCAGTCCGGGAACGttttaa                                                                                                                                                                                                      >  2:713937/1‑237 (MQ=255)
                                    cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCCCAACCATCCGCCAGGGTGCCGGTGGTTCGGTGCGGGGTTATTTCCCCGGAGGGGAAAGAAGCCGCCATGCCGGGACCGTTTTCa                                                                                                                                                                                                      >  1:394392/1‑239 (MQ=255)
                                    cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                                                                                                                                                      >  1:370422/1‑239 (MQ=255)
                                     tcaTGCCGGAGCGCCTTCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATTCTGTTTCTTATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                                                                                                                                                      <  1:708628/237‑1 (MQ=255)
                                     acaTGCCAGAGCGCCTGCGCCTTCGGGGACGACTTTAGTGAGCTTAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGAGGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCCCAACCATCCGCAAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                                                                                                                                                      <  1:72659/238‑1 (MQ=255)
                                      caTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAAACATCACCCAGGGTGCCGGTGGTTCGGTGCGTGGTTATTTGCCCGGAGCGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGa                                                                                                                                                                                                    >  1:497866/1‑239 (MQ=255)
                                              agCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGc                                                                                                                                                                                            >  1:296652/1‑239 (MQ=255)
                                                  cttgcttctTCGGTGCGCATTTTTTTGCGCGAAATTAAGGCAATCAATATTTTTCTGATTTTGTCTTCGGTGCCCAGCAGGTGTGTCACGAATATTTCAGGAACGTTTTGGAAAGTTAAATTACCCGGGCGGGAGACATCAAAACCAGCCCCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAAt                                                                                                                                                                                        <  2:493257/232‑1 (MQ=255)
                                                   ctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCTGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATa                                                                                                                                                                                       >  1:306737/1‑239 (MQ=255)
                                                   ctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCCAATa                                                                                                                                                                                       >  1:870416/1‑239 (MQ=255)
                                                                gaacgacTTTATTGCGATGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt                                                                                                                                                                            <  1:668946/236‑1 (MQ=255)
                                                                              cgcTGAATTAAGGCAATCATACTGTTTCTGTTTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAGGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACACCAATAACACGGATGGTCGGGTTAATGAAttt                                                                                                                                                             >  1:294068/1‑239 (MQ=255)
                                                                              cgcTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCACATAACACGAATGGTGGGGCTAAGAGAttt                                                                                                                                                             >  1:448064/1‑239 (MQ=255)
                                                                                    aTTAAGGCATTTATTCTTTTTCTGATTTCTTGTTCGCTTACCAGCCCGATTTCATCGACAATTTCACGAACGTTTTCGTAAGTTAAATTCCCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCCCCGGAGGGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGc                                                                                                                                                       <  2:520224/239‑1 (MQ=255)
                                                                                    aTTAAGGAAATCATACTGTTTCTGATTTCGGCTTCGCTGACCAGCACGATGTCAACGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGc                                                                                                                                                       <  1:611046/239‑1 (MQ=255)
                                                                                             aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCCCaa                                                                                                                                               >  1:462290/1‑238 (MQ=255)
                                                                                             aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAAACCGCATGGGTGGGTTAAAAGATTTAAtttcacccccaaa                                                                                                                                              >  2:790026/1‑228 (MQ=255)
                                                                                                      tttCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACAAGCAAt                                                                                                                                     >  1:559793/1‑239 (MQ=255)
                                                                                                                cGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGCCTGTACGCCAATAAAACGAATGGTCGGGTTAAAAGATTTAATTGCCACCGCAATAACAGCAAATAAACCGcca                                                                                                                           >  2:181948/1‑239 (MQ=255)
                                                                                                                cGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGca                                                                                                                            >  1:158942/1‑237 (MQ=255)
                                                                                                                           aCCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACGACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCACTAACACGCATGGCCGGGTTAAAAGATTTAATTGCCACCGAAAACACACCAATTAAACCGcccccacacatggg                                                                                                                >  2:337625/1‑232 (MQ=255)
                                                                                                                                  cacttGTCATCGAATTATTCCCGAAAGATTTCGTAAGTTAAATTCCCGGGGGGGGACAAATCCAAACACACCGACGGGGTGCCGGTGGTTGGGTGCGTGGTTTTTTCCCGGGAGGGGAAAGAACCCGCCATGCCGTTAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAAt                                                                                                          <  2:709339/235‑1 (MQ=255)
                                                                                                                                      tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                                                                                                                                                          <  1:205225/107‑1 (MQ=255)
                                                                                                                                      tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                                                                                                                                                          >  2:205225/1‑107 (MQ=255)
                                                                                                                                      tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCCCCACCAATTGGCACAAACACg                                                                                                      >  1:151813/1‑239 (MQ=255)
                                                                                                                                      tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACg                                                                                                      >  1:281516/1‑239 (MQ=255)
                                                                                                                                       ttcagcggattaTTCACGAACGATTTCGAAAGTTAAGTTCGCCGGGGGGGAGACCGCACAAACCACCGCGGGGGTGCCGGGAGTTCGGTGCGTTGTTATTGCCCCGGGGTGGAAAGAGGCCGCCACGCCGTGAACGTTTTCAGACTGTACGCCAATACCACGAATGGTAGGGTTAATAGATTTAATTGCCCCCGCAATCCCAGCAATTAAACCGCCACCACCAATTGGCACAATCACg                                                                                                      <  2:754442/227‑1 (MQ=255)
                                                                                                                                          aTCGACTAATTCACGACCGTTTTCGAAAGTTAAATTCCCCGGGCGGGAGACCTCCAACCCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTAt                                                                                                  <  1:610376/239‑1 (MQ=255)
                                                                                                                                           tCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCACCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAACTACGGTATc                                                                                                 >  1:418916/1‑239 (MQ=255)
                                                                                                                                             gtatAATTCACGAACGTTTCCTTAAGTTAAATGCCCCGGGCGGGACAATTCACACCCCTCCGCCAGGGTGCCGGTAGTCCGGTGCGTGTTTATTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCGGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGa                                                                                               <  1:56291/236‑1 (MQ=255)
                                                                                                                                              gcaaaTTCAGGAACGTTTTCGTAAGTAAAATTCCCCGGGCGGGAGAAATCACACCCAGCCGCAAGGGGGCCGGTAGTGCGGTGCGGGGTTATTTCTCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGa                                                                                               <  1:790026/235‑1 (MQ=255)
                                                                                                                                               ttatttCACGAAGGATTTCGTAGGTTAATTTCCCGGGGCGGGAGACATCAAAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGAc                                                                                              <  1:713937/234‑1 (MQ=255)
                                                                                                                                                tAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCCCCAATTGGACAAATCACGTTACCGaaa                                                                                             >  2:513294/1‑236 (MQ=255)
                                                                                                                                                  aTTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGAcatca                                                                                          >  1:229874/1‑239 (MQ=255)
                                                                                                                                                  aTTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGAAATTAAACCCCCACAACCAATTGTCACAATCACGTTATCTCcatct                                                                                          >  1:560664/1‑238 (MQ=255)
                                                                                                                                                              tttCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGAATTAATTGCAACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGCCACAAAAAAGATCtcca                                                                              >  2:589306/1‑236 (MQ=255)
                                                                                                                                                                 ttagaGTTGAATTGACCGGGGGGGGAAACATCCAACCCTGCCGAGGGGGGGCCGGGAGTTCGGTGCGGTGTTTTTTGCCGGGGGGGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCCAATAACACAAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCAt                                                                            <  2:281516/235‑1 (MQ=255)
                                                                                                                                                                 cGTAAGTTAAATTCCCCGGGGGGGAGACATCACAACCATCCGCCGGGGTGCCGGTAGTTGGGTGCGTGGTTATTTCCCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATa                                                                           <  1:275416/239‑1 (MQ=255)
                                                                                                                                                                      gTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCACTAAttt                                                                       >  1:84423/1‑238 (MQ=255)
                                                                                                                                                                      gTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACACCCAATTGGCACAATCACGTTATCGACATCATAGAGACCTCCCAAAATTTc                                                                      >  2:624078/1‑239 (MQ=255)
                                                                                                                                                                                cccGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCAACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCAAAATCACGTTATCGACATCATAGAGATCTTCCAAAATTTCccaaccaa                                                              >  2:414964/1‑238 (MQ=255)
                                                                                                                                                                                               tCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAAAGATCTTCCATAATTTCCAAACCAATCGTCCCCTGGCCAGc                                              >  2:612259/1‑239 (MQ=255)
                                                                                                                                                                                                 caacccctCCGCCAGGGGGCCGGTGGTTCGGTGGGTGGTTATTCCCCCGGAGGGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCaa                                            <  1:181948/232‑1 (MQ=255)
                                                                                                                                                                                                        tCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                                      >  1:45779/1‑238 (MQ=255)
                                                                                                                                                                                                        tCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCAACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAACCGTTCCCTGGCCAGCAATCACttc                                     >  1:793343/1‑238 (MQ=255)
                                                                                                                                                                                                                      ggttttttgggTGCGTTTTTTTTTCCCGGGGGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGTCTGTACGCCAATAACCCGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa                        <  2:45779/230‑1 (MQ=255)
                                                                                                                                                                                                                           gttgggggggtgtTTATTTCTCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAAATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTgg                   <  2:158942/230‑1 (MQ=255)
                                                                                                                                                                                                                              gggTGGGGGGTTATTGCCCGGGAGGGAAAAGAGGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGAt                <  1:13755/238‑1 (MQ=255)
                                                                                                                                                                                                                                            tttCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGCCATCATAGAGATCTTCCATAATTTCCAGACACATCGTTCCCTGGCCAGCAATAACTTTCGGATCACCGTAAGGTGGAGTAAAAATACGGcc    >  1:763565/1‑238 (MQ=255)
                                                                                                                                                                                                                                              tctcCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt  >  1:595886/1‑238 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
GAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT  >  NC_000913/3265125‑3265595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: