Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,280,131 A→G 12.7% E51G (GAG→GGG)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,1310AG12.7% 172.1 / 2.7 71E51G (GAG→GGG) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (39/20);  new base G (8/2);  total (49/22)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCT  >  NC_000913/4279899‑4280363
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         
ttttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGGCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTg                                                                                                                                                                                                                                      <  1:645467/238‑1 (MQ=255)
 tttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGGGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGTTGCGCTACTGGCGTGGCCGCGGTTTGGTTTGCATGTGGAGTTTGa                                                                                                                                                                                                                                     >  1:188621/1‑238 (MQ=255)
  tttggATATTTTGAAAAAAATTAGAATTCAGGCATAACGTTTTTCTCAGATCAAAAAAAAAATCGCAAACAGGGAACGCATGGAAATCTTCTTCCCCATACTGATAATGCCCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATc                                                                                                                                                                                                                                   <  2:454589/235‑1 (MQ=255)
  ttGCGATATTTGGAAAAAAATGAGAATTCCGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATc                                                                                                                                                                                                                                   <  1:264406/239‑1 (MQ=255)
  ttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGGGTTTGAt                                                                                                                                                                                                                                    >  1:227872/1‑238 (MQ=255)
   tGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGTTGCGCTACTGGCGTGGCCGACGTTTGGTTTGAATGGGGAGTTTGAt                                                                                                                                                                                                                                    >  1:520146/1‑237 (MQ=255)
   tGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGGGTTTGAt                                                                                                                                                                                                                                    >  1:248045/1‑237 (MQ=255)
   tGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGAt                                                                                                                                                                                                                                    >  1:398636/1‑237 (MQ=255)
        tatTCTGAAAAAAATTGGAATTCAGGCATAACGGCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCGTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAc                                                                                                                                                                                                                            <  1:570512/239‑1 (MQ=255)
        tatTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGaa                                                                                                                                                                                                                             <  1:196928/238‑1 (MQ=255)
             tGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCttt                                                                                                                                                                                                                       <  1:673261/239‑1 (MQ=255)
               aaaaaaaTGAGACTTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGCTAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCACGCTTCCGCTTATGCAAATCGCCATCGGTGCGTTACTGGCGTGGCCGACGTATGGTTTCATGTGTGAGGTTGATCCTGAGACTCtttt                                                                                                                                                                                                                      >  2:65607/1‑239 (MQ=255)
                    aaTGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGGGTTTGATCCTGAACTCCTTTTAGTCt                                                                                                                                                                                                                >  1:661320/1‑239 (MQ=255)
                                    tAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGtt                                                                                                                                                                                                >  1:448976/1‑239 (MQ=255)
                                    tAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGCCCGACGTTTGGTTTGCCTGTGGAGTTTGGTCCTGAACCCTTTTTAGTCTTGTTTATCccgcctt                                                                                                                                                                                                 >  1:388991/1‑236 (MQ=255)
                                       cGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCt                                                                                                                                                                                             >  1:267374/1‑239 (MQ=255)
                                             tctcAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATc                                                                                                                                                                                                                                   <  1:370945/196‑1 (MQ=255)
                                                   aTCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGCCGTTTGGTTTGCATGTGGGGTTTGATCCTGAACTCTTTTTAGGCTTGTTTATCCCGCCGTTGCTGTTCGCttgtgg                                                                                                                                                                                 >  1:826828/1‑234 (MQ=255)
                                                               ctcccaacgcgggACCGCAAGGAAACTTTCTTAACCATTTTGATAATGGCCCTGGGGGGTCCGGTGTCTGGGGGGGTCACGCGTGGCATCCCCGGTCAGACCCCGCTGCCGTTTATGCAACTCGCAATCGGTGCGCTACGGGCGTGGCGGACGGTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGcc                                                                                                                                                                     <  2:730064/229‑1 (MQ=255)
                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAc                                                                                                                                                                                                                            >  2:109637/1‑182 (MQ=255)
                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAc                                                                                                                                                                                                                            <  1:109637/182‑1 (MQ=255)
                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGATGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCCTGTGGAGTTTTATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCCCTGATGGCTGGGAAACCCCg                                                                                                                                                                    >  2:328102/1‑238 (MQ=255)
                                                                           gAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAAtt                                                                                                                                                         >  1:203332/1‑239 (MQ=255)
                                                                             ccGCATGGAACTCTGCTTCACCATACTGATAGTGCCCCTCGTGGTCTCGCTGCCCGGGGTGGTCACCCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAAt                                                                                                                                                          <  1:43531/235‑1 (MQ=255)
                                                                                cATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAAAGCCGACCCGTGAATTTCTCg                                                                                                                                                    >  1:713438/1‑239 (MQ=255)
                                                                                  tGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGGGTTTGATCCTGGACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGGTGGCTGGAAAACGCCGAACCGGGAAttttcggaa                                                                                                                                                  >  1:231852/1‑233 (MQ=255)
                                                                                       aTCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTTTCCCGCCGTTGCTGTTCGCTGTTGGGTGGAAAACGCCGCCCCCGGAATTtctccacaa                                                                                                                                                >  2:43531/1‑231 (MQ=255)
                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTATCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCgaga                                                                                                                                       >  1:745873/1‑237 (MQ=255)
                                                                                                  cATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGCGCGAttt                                                                                                                                  >  1:333723/1‑239 (MQ=255)
                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCTGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATTTGGCGTTTGTTCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAAGCCCGACCCGTGAATTTTCCGAACATGGGCGGGGGTttttgtg                                                                                                                              >  2:837886/1‑233 (MQ=255)
                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGcc                                                                                                                            >  1:649776/1‑238 (MQ=255)
                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGcc                                                                                                                            >  1:652648/1‑238 (MQ=255)
                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGCCTTGTTTTTCCCGCCGTTGCTGTTCGCCGATGGCGTGAAAACGCCGACCCGGGAAATTTTCAAACATGGTCGAGAGATTTTCGGccc                                                                                                                           >  2:107869/1‑238 (MQ=255)
                                                                                                           aaTGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGGTTGATCCTGAACTCTTTTTAGTCTTTTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGAACCGTGAATTTTTCGAACATGGTAGGTGGATTTTCGGCCTc                                                                                                                          >  1:523374/1‑238 (MQ=255)
                                                                                                              gACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGGGGGATTTTTGCCCTCtcgc                                                                                                                      >  1:568097/1‑239 (MQ=255)
                                                                                                              gACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGGTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTAACTTTTTGCAACATGGTCGCGGGATTTTTGGCCTccc                                                                                                                        >  1:568111/1‑235 (MQ=255)
                                                                                                                   tCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTCCTGGCGTGGCCGCCGTTTGGTTTGCATGTGGGGTTTGGTCCTGAACGCTGTTTAGTACTTTTTTTCCCGCCGTTGCTGTTCGCTGTTAGCCGGAAAACACCGGCGCGTAAAGTTTCCGGAAATGGGGGGGAGAATTTCGGCCTcgcggggggg                                                                                                                 >  2:751653/1‑233 (MQ=255)
                                                                                                                   tCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGCGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTTTCGAAAATGGGCGAGAGCTTTTCGGCCACtcgctgtc                                                                                                                  >  1:344315/1‑236 (MQ=255)
                                                                                                                   tCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGGAAACGCCGACCCGTGAATTTCTCGAACATGGTCGGAGGATTTTCGGCCTccgcctggc                                                                                                                  >  1:892510/1‑230 (MQ=255)
                                                                                                                   tCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggc                                                                                                                  >  1:250421/1‑238 (MQ=255)
                                                                                                                    cGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGACCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGGGTTTGATCCTGAACTCTTTTTAGTCTTTTTTATCCCGCCCTTGCTTTTCGCGGCTGGCCGTAAAACGCCGACCCGTTAATTTCTCGAACATGGTCGCGGGGTTTTTGGCCCCcgcctggcgc                                                                                                                >  2:2982/1‑239 (MQ=255)
                                                                                                                     cgtttctcGCTTTCCCGGGGTGGTCCCGCGTTTCCGGCCTTTTCAGATCCCGCTTCCGTTTATGCAAATCCCCATCGGTGCTCTCGTGGCGTGGCCGACTTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggcgc                                                                                                                <  2:713438/235‑1 (MQ=255)
                                                                                                                           tcgcctgccgggggggGTCTCTGGTTTCAATCCTTTCCAGATCCCTCTTCCGCTTAGGCAAATCGCCATGGGTGCGCTATGGGCGTGCCCGACGTTGGTTTGGCATGTGGAGTTTGATCCTGAACTCTTTTCAGTCTGGTTCATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtg                                                                                                           <  2:826828/228‑1 (MQ=255)
                                                                                                                           tcGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGGGATTTTCGGCCTCGCCCTGGGGTtgggggg                                                                                                         >  1:582988/1‑238 (MQ=255)
                                                                                                                               gttCCGGGGTTGTTTCTCCGTGTCATGCCTTTTGAGTCCCCGTTTCCGTTTAGGCAAATCGCCATCGGTGCGCTACTGGCGGGGCCGACTTTTGGTTTGCATGTGGAGTTTGATCCTGAACTTTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTggtggtggt                                                                                                      <  2:248045/237‑1 (MQ=255)
                                                                                                                                    cGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGGGCTGTGGGGGGGCACCg                                                                                                 >  1:454354/1‑239 (MQ=255)
                                                                                                                                       ggtggtCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTTTCGAACATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGGGGTCACCGTgg                                                                                              >  1:581353/1‑239 (MQ=255)
                                                                                                                                        gtggtCACCCGTGTCATGCCCTTTCAAATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTTCTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTgg                                                                                              <  1:140766/238‑1 (MQ=255)
                                                                                                                                            tCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTTTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGGGGGGGTTACCGTGGTCg                                                                                           >  1:863371/1‑237 (MQ=255)
                                                                                                                                             cACTCGTGTTCTGCCCTTTCAGTTCCCGTTTCCTGTTTTGCAAATCGCCAGCGGTGCGCTACGGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCa                                                                                        <  1:525770/239‑1 (MQ=255)
                                                                                                                                             cACCCGTGTCATGCCCTTACAGACCCCCGTTCCGCTTAGGCAAATCGCCATCGGTGCGCTACTGGCGTGTCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCa                                                                                        <  1:220103/239‑1 (MQ=255)
                                                                                                                                              aCTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGGGGTAACCGTGGTCGGCAt                                                                                       >  1:797691/1‑239 (MQ=255)
                                                                                                                                                         tccccTTTCAGGCCCTGTTTCCGGTTATGCAAATCGCCATGGGTGCGCTACGGGGGGGGCCGATTTTTGGTTTGCTTGTGGGTTTTGATCCTGAACTTTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCtt                                                                             <  2:203332/237‑1 (MQ=255)
                                                                                                                                                           cctttgctgttcccgttcccgtTTTTGCAATTGGCCTGCGGGGCGCTGTTGGCGTGCCGTACTTTTGTTTGTCTGGGGGAGTTTGTTCCTGAACCTTTTTTAGTCTTGTTTCTCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTAt                                                                           <  2:388991/229‑1 (MQ=255)
                                                                                                                                                              tttCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGGGGGGGTCACCGTGGTCGGCATTGGCTTCCTTAtttt                                                                        >  1:243830/1‑238 (MQ=255)
                                                                                                                                                              ttccgaaccccGCTTCCGTTTATGCAAATCGCAATCGGTGCGCTACTGGCGTGGCGGCCGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTa                                                                        <  1:76797/231‑1 (MQ=255)
                                                                                                                                                                    aTCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGCCCCGTGAATTTCTCGAACATGGTCGCGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGCTGGCATTGGCTTCCTTTTTTTCTgggg                                                                  >  1:533/1‑238 (MQ=255)
                                                                                                                                                                        cgcttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGTAAACGCCGACCCGTTAATTTCTCGAACATGGTCGAGAGATTTTCCGCCTCGCGCTGGCGCTGGTGGTGGTCCCCGTGGTCGGCATTGGCTTCCTTCTTTACTTGggggtg                                                              >  2:872127/1‑239 (MQ=255)
                                                                                                                                                                          cttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGGGATTTTCGGCCTCGGCCTGGCGCTGGTGGTGGTCACCTGGGTCGGCATTGGCTTCCTTATTTAGTggggggggag                                                            >  2:419831/1‑235 (MQ=255)
                                                                                                                                                                          cttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGGGGTGcc                                                            >  1:113685/1‑239 (MQ=255)
                                                                                                                                                                          cttccgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAAAGCCGACCCGTGAATTTCTCGAACATGGTCGAGGGATTTTCGTCCTCGCGCTGGGCGTGGTGGTGGTGACCTCGGTCGGCAGTGGCTTCCTTATTTACTGggtgtgtcc                                                            >  1:123609/1‑234 (MQ=255)
                                                                                                                                                                               cttatcgCAAATCCCCATCGTTACCTTCCTGGCGGCTCCGGCGTTTGTTTTTCTTGTGTGTTTTGATCCTGCACTCTTTGTAGTCTTTTTTCTCCCGCCGTTGCTGTTCGGTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCa                                                       <  2:188621/233‑1 (MQ=255)
                                                                                                                                                                                                              gCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGGGCCGGGCATTCCCCTGATCCCTGCCTTTTCGCTggcggc                        >  1:165992/1‑239 (MQ=255)
                                                                                                                                                                                                              gCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGGGGTCACCGTGGTCGGCATTGGCTTCCTTCTTTACTGGGTGGTGCCGGGCCTTCCGCTGCTCCCTGCCTTTTCCCTgggggc                        >  1:565541/1‑239 (MQ=255)
                                                                                                                                                                                                                   gccggctgttggttggGATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTg                    <  1:675928/230‑1 (MQ=255)
                                                                                                                                                                                                                   gCCGGCGTTTGGGTTGCAAGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACTCCGACCCGTGAATTTCTCGCACAGGGTCGCGCGATTTTCGGCCTCCCGTCTGCGCTAGTGGTGGTCACCGGTGTCGGCATTGGCTTTCTGATTTACTGGGTGGGGCCGGGCATTCCCCTGGTGCCCTCATTTGCGCCGGgggaggggg                   >  2:478991/1‑236 (MQ=255)
                                                                                                                                                                                                                   gCCGACGTTTGGTTTGCATGTGGGGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGt                     >  1:582466/1‑237 (MQ=255)
                                                                                                                                                                                                                   gCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGGGCCGGGCATTCCGCTGATCCCTGCCTTTCCGCTTGCGGGGGTgg                   >  1:158180/1‑238 (MQ=255)
                                                                                                                                                                                                                     cGACTTTTGGTTTGAATGTGGAGTTGGATCCTTAACTCTTTTGAGTTTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCt                  <  1:751653/238‑1 (MQ=255)
                                                                                                                                                                                                                                    aTGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGGGATTTTTGGCCTCGCGCTGGCGCTGGTGGGGGTCACCGTGGTCGGCATTGGCTTCCTTGTTTCCTGGGTGGTGCCGGGCTTTCCGCGGATCCCTGCCTTTGCGCTGGCGGCGGGGCCTTCTcctcgcgctatt  >  2:31851/1‑229 (MQ=255)
                                                                                                                                                                                                                                          gAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTggtggtggt                                                                                                      >  1:62102/1‑134 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         
TTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCT  >  NC_000913/4279899‑4280363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: