Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,487,250	C→T	14.2%	L345F (CTC→TTC)	lptF →	lipopolysaccharide export ABC permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,487,250	0	C	T	14.2%	198.3 / 3.3	88	L345F (CTC→TTC)	lptF	lipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base C (29/44);  new base T (11/2);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 4.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAT  >  NC_000913/4487017‑4487478                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       acgggCGTATCACGTTGGTATTCACCGTGTTTATGATGGCCTTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAAc                                                                                                                                                                                                                                   <  1:250379/236‑1 (MQ=255)     gCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTACAGGG‑AAACCGGACCCGACGCTGGGGGTTGGGAACACTAACCAGATTTATTT‑G‑GGTTTAG‑GGATTG‑TTGTAAACCctt                                                                                                                                                                                                                               >  2:360120/1‑239 (MQ=255)     gCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGTTGTGGGCCGCTAACCTGGTTTATCT‑T‑GCTTTAT‑CGATTG‑GTTTCAACCttt                                                                                                                                                                                                                               >  1:379361/1‑239 (MQ=255)          tCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGTCGGTAACGGT‑AAGCTGGGCCCGACGCTGTGGATGTGGGCCGTTAACCTAGTTTATCT‑G‑GCTTTAG‑CGATTG‑TTTTCAACCTTTgggg                                                                                                                                                                                                                           >  1:125886/1‑237 (MQ=255)          tCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACCCTGTGGAGTTGGGCCGGTAACCTGATTTTTCT‑G‑GCTTTTT‑CGATTG‑TTCTCAACCTTTGGGac                                                                                                                                                                                                                          >  1:483033/1‑239 (MQ=255)               ttGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGCAAAGGT‑AAGATGGACCCGACGCTGTGGAGTGGGAACGGTACCCTGATTTTGTA‑G‑GCTTTTA‑TCATTG‑TTTTCGAACTTTggggacccg                                                                                                                                                                                                                      >  2:206077/1‑232 (MQ=255)                   tATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGATAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGCCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGCTTG‑TTTTCAACCTTTGGGACACCggtccc                                                                                                                                                                                                                 >  1:466433/1‑234 (MQ=255)                        aCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACTCACGTCAGGGACGCGTACTGTCGATGCTGTCAGCCATGTTGCTGTATCTACTTTTCTTCCTGATCCCGACCTCCCTCAAATCGA‑A‑CGGCGGTTTCGGTGAAGC‑GGGGCCAACTCCGTGGTCAGGGACCGGTATCCTGAATTAACT‑G‑GCTTTCC‑GTCGTT‑TTCTCATACTTTGGGacacacggacggcgc                                                                                                                                                                                                             >  2:191190/1‑227 (MQ=255)                        aCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑C‑CGGCGGTAAAGGG‑AAACTGGACCCGGCGCTGTGGATGTGGCGCGGTAAACTGCTTTTTCT‑G‑GCTTTAG‑AGATTT‑TTTTCAACCTTTTGGCGACccgg‑ccgggcac                                                                                                                                                                                                           >  2:194012/1‑235 (MQ=255)                          cGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGA‑TAGCTGGACCCGACCACTTGGATGTGGACCGTTACACTGATTTATTT‑G‑GGTTTGG‑CGATTG‑TTTTCAAACTTTTGTAGAACGTG‑CCGGtgccc                                                                                                                                                                                                          >  2:779687/1‑234 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGT‑A‑CGGCGGTAAAAGT‑AAACTGGACCCGACCCCGTGGATGTGGACCGTTAACCCGATTTATTT‑G‑GCGTTCG‑CGCTTT‑TTTTTAACCTTTGGGAAACCGTG‑GCGGGCcggcgc                                                                                                                                                                                                       >  2:766119/1‑239 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGTCCCCCCGCCGTGGATGTGGACCCTTAACCTGATTTATTT‑G‑GATTTAG‑CGCTTG‑TTTTTACCCCTTTGGACACCGTA‑CCGGGgcgccg                                                                                                                                                                                                        >  2:516265/1‑238 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGGGGATGTGGACCGTTAACCTGATTTATTT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGgccgccc                                                                                                                                                                                                        >  1:87401/1‑237 (MQ=255)                                    atgGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATTT‑G‑GCTTTTG‑GGATTG‑TTTTCAAACTTTGGGACACCGTG‑CCGGTCCCCCCCCTgcg‑cg                                                                                                                                                                                               >  1:370498/1‑238 (MQ=255)                                        cACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTCAAAGT‑AAACTGGAACCGACCCCTTGGATGTGGACCGTGAAACCGATTTTTCT‑T‑GGTTTAGAGGATTG‑TT‑TCACCCTTTTGGACACCGTGCCCGGCCCGCC‑CCCGCG‑CGccggg                                                                                                                                                                                          >  2:279268/1‑236 (MQ=255)                                        cACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGGTGAGGTGGACCGCTAACCTTATTTTTCT‑T‑GCTTTGT‑CGATTG‑TTTTCACCCTTTGGGGACCCCTG‑CCGGTCCCCCCCCTCCG‑CGCCa                                                                                                                                                                                            >  2:312482/1‑237 (MQ=255)                                             aTGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCCGGTTTATCT‑G‑GCTTTTG‑CGATTG‑TTCTCAACCTTTGGGACACCCTT‑CCGGTCCCCCGCCTTCG‑CGCCCGttttt                                                                                                                                                                                      >  1:389605/1‑238 (MQ=255)                                                    tcccGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCa                                                                                                                                                                                                                                     <  1:344622/184‑1 (MQ=255)                                                    tACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGGTGACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGGG‑CCGGGCCGCCCCCTGCG‑CGCCAGTTTTTTGCGGaaa                                                                                                                                                                              >  1:346943/1‑239 (MQ=255)                                                    tACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAAGT‑GAACTGGACCCGCCCCTCTGGATGGTGAACGTTAACTTTCATTTTCTAG‑GTTTAAG‑CG‑TTG‑TTTTTAACCCTTTGGAAACCGGT‑GCGGGCCGCCGGCCGCG‑CGGCCGATTTTTGCGGaaa                                                                                                                                                                              >  2:436852/1‑239 (MQ=255)                                                     aCCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGG‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCCTG‑CCGGTCCGCCGCCTGCG‑GGCCAGTTTTTCCCGTAAAg                                                                                                                                                                             >  1:236522/1‑239 (MQ=255)                                                         cTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCTCGTAAAGGAGc                                                                                                                                                                         >  1:135373/1‑239 (MQ=255)                                                                 ggTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGGAAAGGG‑AACCTGGACCCCACCCTGTGGTTGGGGACCGGTTACCTGATTTATTT‑G‑GCTTTTG‑CGATTGTTTCTCACACTTTGGTACA‑CGTG‑CCCGTTCGCCGCCCGCGCCGCAAGTTTTTGACGTAAgggggggcggggg                                                                                                                                                                  >  2:768972/1‑229 (MQ=255)                                                                  tttACCCCCCGTCAGGGACGGTTATTGTCGATGCTTCCAGCCAGGCTGCTGTTCTTGCTTTTCTTCCTGATCCAGACCCCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTTTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATg                                                                                                                                                                <  2:346943/238‑1 (MQ=255)                                                                                    cggttcttttCGTTGTTGCCAGCCTTTCTGTTTTTTCTTTTTTTCTTCCGGATCCAGCCCTCCCGGAAATCGA‑A‑CGGCGGTAAAGTT‑AAGCTGGCCCCGACGCTGTTGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                                                                                                                                              <  2:370498/230‑1 (MQ=255)                                                                                       gTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAc                                                                                                                                           <  1:600812/239‑1 (MQ=255)                                                                                             gggagggtcccagccaTGCTTTTGTTTCTATTTTTCTTCCTGATCCAGCCCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGCCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                                                                                                                                     <  2:716935/230‑1 (MQ=255)                                                                                             gctttccccccaggctTGCTTCTTTTTCTTCTTTTCTGACTGACCCAGCCCCTCCTTAAACTGG‑A‑CGGCGGTAATGGT‑ACGCTGGCCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                                                                                                                                     <  2:711363/230‑1 (MQ=255)                                                                                                   ctgcCAGCCATGCTGCTGTATCTTCTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTg                                                                                                                                                      <  1:268165/216‑1 (MQ=255)                                                                                                      cccggcaTGCTGCTTTTACTACTTTTCTTCCGGATCCAGACCTCCCTGAAATCGG‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa                                                                                                                            <  1:332018/234‑1 (MQ=255)                                                                                                       cagccaTGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa                                                                                                                           <  1:875181/239‑1 (MQ=255)                                                                                                                 gctttaacgaCTTTTCCTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTcac                                                                                                                 <  1:61896/230‑1 (MQ=255)                                                                                                                  ctgTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCCTTTGGCGTACTTGACCGCTATATCGGGAAAACAATTTTcacc                                                                                                                >  1:327733/1‑239 (MQ=255)                                                                                                                     ttttttcTTTTCTTCCTGATCCAGACCTCCCTGAAACGGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCGCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCaccacc                                                                                                             <  1:809886/233‑1 (MQ=255)                                                                                                                     tATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCCGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCCGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGTGGTACTTTACCGCTATATCGGGAAAACCATTTTCaccacc                                                                                                             >  1:740825/1‑239 (MQ=255)                                                                                                                       tctttttttCTTCCGGAGCCAGACCCCCCAGAAATGGA‑G‑CGGTGGTAAAGGG‑AAGCGGGACCCGTCGCTGTGGATGTGGACCGTTAACCTGATTTAGCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCACCCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACcat                                                                                                           <  2:591329/234‑1 (MQ=255)                                                                                                                       tcttctTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCGGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACcat                                                                                                           <  1:83703/239‑1 (MQ=255)                                                                                                                       tcttctTTTCTCTCTGCGCCAGACCCCCCT‑‑AATCGACACCGGCGGCAAAGGT‑AATGTGGCCCCGACGCTGGGGTGTTGGACCGTTAACCGGATTTATCT‑G‑GCTGTAG‑CGATTG‑TTCTCAACCTTGGGGACGCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCTCCACcat                                                                                                           <  2:455770/239‑1 (MQ=255)                                                                                                                       tCTACTTTTCTTCCTGGTCCAGACCCCCCTGAAATCGA‑A‑GGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACcat                                                                                                           <  1:204046/239‑1 (MQ=255)                                                                                                                         tACTTTTCTCCCTGACCACGACCCCCCGGAAAGCGA‑A‑CGGCGGTAAGGGT‑AAGCTGGCCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACcatca                                                                                                         <  2:112181/239‑1 (MQ=255)                                                                                                                          aCTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTTGGACACCGTG‑CCGGTCCGCCGCCTCCG‑CGCCAGTTTTTTGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTTACCGCTATATCGGTAAAACCATTTTtaccccactcat                                                                                                        >  1:633978/1‑231 (MQ=255)                                                                                                                                cttcCCGCTCCAGGCCTCCCTAAAAGCGA‑C‑CGGGGGTAAAGGT‑AAGCTGGTCCCGAGTCTGTGGAGGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGac                                                                                                  <  2:526814/239‑1 (MQ=255)                                                                                                                                 ttcCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGTTAAAGGT‑AAGCTGGCCCCGACGCTGTGGATGTGGACCGTTAACCTTATTTACCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGaca                                                                                                 <  1:812990/239‑1 (MQ=255)                                                                                                                                   cccgctcccgtccTCCCTGACATCGA‑G‑CGGCGGTAAAGGT‑AGGCTGTACCGGACGTGTTGGATGTGGCCCGTTAACCTGATTTATCG‑G‑GCTTTAG‑CGATTG‑TTCGCATCCTTTGGGAAACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTTGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACt                                                                                               <  2:229984/228‑1 (MQ=255)                                                                                                                                         ccAGTCCCCCCGTAAATCGA‑A‑CGGGCGTAAAGGT‑AAGCTTGCCCCGGCGTTGTGGATTTTGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCAt                                                                                         <  2:236522/239‑1 (MQ=255)                                                                                                                                                 cccTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCCGTTTTTCGCGTAAAGGAGCGGGGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCCTGCTGgtg                                                                                  >  1:614621/1‑238 (MQ=255)                                                                                                                                                  ccTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCCTCCTGATGCCACTGTTCATGCttgggt                                                                                 >  1:68637/1‑233 (MQ=255)                                                                                                                                                                               aGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCCTTATCAAGTTTTTCg                                                      >  1:197026/1‑239 (MQ=255)                                                                                                                                                                                  tGGACCCGACGCTGGGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTGTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGAt                                                    <  1:537292/238‑1 (MQ=255)                                                                                                                                                                                  gggACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTGAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGAt                                                    <  1:516265/237‑1 (MQ=255)                                                                                                                                                                                     aCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAG‑TTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCt                                               >  2:4398/1‑239 (MQ=255)                                                                                                                                                                                      cccGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGAAACTGTTCATCCTGGGGTCGCTGGCGGGCATTTTCAAGtttttgcttccgcg                                               >  2:471592/1‑228 (MQ=255)                                                                                                                                                                                      cccGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTCGGGACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAACGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAACGCTATATCGGTAAAACTATTTTCACCACCATCCTGATGACACTGTTCAGTCTGTGGGCGTTGTCGGGCATTAACAAGTTTGTGCATCa                                                  >  2:335840/1‑236 (MQ=255)                                                                                                                                                                                                   gtttgtcccGTTAACCTG‑TTTTTAT‑GTGCTTTAG‑GCATTG‑TTCCTAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  2:466433/232‑1 (MQ=255)                                                                                                                                                                                                    tgtggaCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  1:71254/238‑1 (MQ=255)                                                                                                                                                                                                    tgtggaCCGTTAACCTGATTTAGCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑GCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  1:526978/238‑1 (MQ=255)                                                                                                                                                                                                     gtggcCCTTTACCCTGGTTTTACT‑G‑GCTTTAT‑GCTGTG‑TTCGCAACCTTTGGGACACCTGG‑CCGGTCCGCCGCCTGCG‑CGCCAGGTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAACCTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCa                                <  2:807535/239‑1 (MQ=255)                                                                                                                                                                                                     gtggaCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGc                                 <  1:88526/238‑1 (MQ=255)                                                                                                                                                                                                      tgtaCCTTTAACCTTATTTTTCT‑G‑GCTTTGG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑GGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:873215/236‑1 (MQ=255)                                                                                                                                                                                                      tggtCCGTTAACCTGATTTATCT‑G‑GCTTGAG‑CTATTG‑TTCTCAACCGTGGGGACGCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTTATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCAGGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:809720/239‑1 (MQ=255)                                                                                                                                                                                                      tggaCCGTTAACCTGATTTATCG‑G‑GCTTTAG‑CGATTG‑GTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:360120/239‑1 (MQ=255)                                                                                                                                                                                                      gggaCCGTTAACCTGATTTATTG‑G‑GTTTAAG‑GGATTG‑TTCTCAACCTTGGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGGGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:191190/238‑1 (MQ=255)                                                                                                                                                                                                       ggaCCTTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:816240/238‑1 (MQ=255)                                                                                                                                                                                                       ggaCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCCCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:181699/238‑1 (MQ=255)                                                                                                                                                                                                        gaCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAggg                             >  1:288181/1‑239 (MQ=255)                                                                                                                                                                                                        gaCCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGACgggggggg                             >  2:877743/1‑234 (MQ=255)                                                                                                                                                                                                          ccGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGGGTCGCGGTCTGGCATTATCAAGTTGTTCGATCAGCTGAAAAAAGCGGGGCAggggg                           >  2:648152/1‑238 (MQ=255)                                                                                                                                                                                                             ttAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGGGtt                        >  1:726723/1‑239 (MQ=255)                                                                                                                                                                                                               aaCCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTac                      >  1:873517/1‑239 (MQ=255)                                                                                                                                                                                                                 ccTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTacga                    <  1:471592/239‑1 (MQ=255)                                                                                                                                                                                                                     aTTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAACAAGCCGGGCAGGGGAGttcgaacgcg                >  2:554900/1‑231 (MQ=255)                                                                                                                                                                                                                          tCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTAAGACGCGTTAg            >  1:90490/1‑238 (MQ=255)                                                                                                                                                                                                                            t‑G‑GCTTTAG‑CGTTTG‑TTCTCACCCTTGGGGCCCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  2:614621/239‑1 (MQ=255)                                                                                                                                                                                                                            t‑G‑GCTTTAG‑CGATTT‑TTCTCAACCTTTGGGACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  1:194012/239‑1 (MQ=255)                                                                                                                                                                                                                            t‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  1:877743/239‑1 (MQ=255)                                                                                                                                                                                                                             gg‑gCTTTAG‑CTGTTG‑TTCCCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  2:125886/238‑1 (MQ=255)                                                                                                                                                                                                                                gCTTTAG‑CGTTTG‑TTTTCCACCTGTGGGCTACCGGG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATAACGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  2:68637/239‑1 (MQ=255)                                                                                                                                                                                                                                gCTTTAG‑CGATTG‑TTCACAACCTTTGGGACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  1:766119/239‑1 (MQ=255)                                                                                                                                                                                                                                gCTTTAC‑GGATTG‑TTCTCAACCGTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  1:127658/239‑1 (MQ=255)                                                                                                                                                                                                                                 gcttttcgtaTTT‑TTCTCAACCTTTGGGACCCCGTG‑CCGGTCCGGCCCCGGCC‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCATCCTTTTGGCGTACTTGCCCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  2:483033/230‑1 (MQ=255)                                                                                                                                                                                                                                 cTTTAG‑CGATTT‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  1:67913/238‑1 (MQ=255)                                                                                                                                                                                                                                 cTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTacg                     >  1:284154/1‑224 (MQ=255)                                                                                                                                                                                                                                 cTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAg      >  1:10380/1‑239 (MQ=255)                                                                                                                                                                                                                                   ttAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGGCCGCCGCCTTCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTGTCACCCCCATCATGATGACACTGTTCATGCTGGGGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGa    <  1:436852/239‑1 (MQ=255)                                                                                                                                                                                                                                    tAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCCGGaa   >  1:294853/1‑239 (MQ=255)                                                                                                                                                                                                                                     aG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGCAACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGCTCACCTGAAAAAAGCCGGGCAGGGGAGTTACGAACGGTTAGCGGCAGGAAt  >  2:221112/1‑239 (MQ=255)                                                                                                                                                                                                                                     aG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAACCCGGGCAGGGGAGTTACGACGCGTTTGGGGCGGGaaa  >  2:711360/1‑238 (MQ=255)                                                                                                                                                                                                                                      tggggttG‑TTCCCAACCTTTGGGACACCGGG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAt  <  2:726723/234‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       ACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCT‑G‑GCTTTAG‑CGATTG‑TTCTCAACCTTTGGGACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAT  >  NC_000913/4487017‑4487478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑