Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,530,174	T→A	12.7%	I24F (ATC→TTC)	sgcC ←	putative PTS system EIIC permease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,530,174	0	T	A	12.7%	237.2 / 2.1	90	I24F (ATC→TTC)	sgcC	putative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base T (51/26);  new base A (2/11);  total (53/37)
Fisher's exact test for biased strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGA  >  NC_000913/4529947‑4530402                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        aagaaCTGGGGTAATCAAGGCCCCAATGGCTGTCGCATAACCCACGCCGGAGTCAGGGCCTGCGCCAACGGCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCGCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCa                                                                                                                                                                                                                              <  1:538509/236‑1 (MQ=255)   tAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCAAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑g                                                                                                                                                                                                                           <  1:759608/239‑1 (MQ=255)      cTGGGATAAACATGGCGCCAATGGGTGGCGCATAACCAACGGCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATc                                                                                                                                                                                                                        <  1:718732/239‑1 (MQ=255)              aTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATgatgat                                                                                                                                                                                                                >  1:872471/1‑239 (MQ=255)               tCATGGCGCCAATGGGTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAC‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATa                                                                                                                                                                                                               <  1:229807/239‑1 (MQ=255)                   ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAA‑CGCCAAAACCAAT‑CCCACCCGTTACCCCCGCCCTAATCGCCTCCAGCCCGGGGATGCGGAAACAAAAGCCAACAAC‑AATACTGATGAAAGGGa                                                                                                                                                                                                           >  2:465265/1‑239 (MQ=255)                    gcgcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATTCC‑GATCATGAATATAGGGAc                                                                                                                                                                                                          >  1:287135/1‑239 (MQ=255)                         aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATACTGATGAAAGGGACGaaaa                                                                                                                                                                                                     >  1:421200/1‑239 (MQ=255)                         aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGCCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATGGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCATTCAC‑GATCATGATGATAGGGACGcaa                                                                                                                                                                                                      >  1:849362/1‑238 (MQ=255)                          aTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCGTAC‑CGCCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGGAAGTAAGGCCAATCCA‑GATCCTGGTGAAAGGGAGGaaaaa                                                                                                                                                                                                    >  2:86769/1‑238 (MQ=255)                                ggcGCATACCCAACGCCGGAGGCAGGGCCTGGGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTAc                                                                                                                                                                                               <  1:131164/236‑1 (MQ=255)                                 tCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAGCGCTCAATCATCCCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCACT‑CCCAACCGTTACCCCTGCCTTATTCGCCCGCAGCCCGGGGATCCGGAACATAAGGACAACCAC‑GAACCAGAAGAAAGGGACGAAAAAGGAAca                                                                                                                                                                                              >  2:91695/1‑237 (MQ=255)                                             cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                                                                                                                >  1:445902/1‑193 (MQ=255)                                             cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCCCCTGCAACCCGGGGATTCGGAAGCAAAAGCCAATCAC‑CATAATGATGATAGGGGCGAAAACGGTACAGCCGAGGGAC‑Agg                                                                                                                                                                                >  2:672100/1‑239 (MQ=255)                                             cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGAATGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                                                                                                                <  2:445902/193‑1 (MQ=255)                                                        gggCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGCTGATAGGGACGAAAACGGTACCCCCGAGAGAC‑AGGATGTAATCaaa                                                                                                                                                                     >  1:814990/1‑239 (MQ=255)                                                               cgcCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAAt                                                                                                                                                               >  1:802080/1‑238 (MQ=255)                                                               cgcCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACACCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑CGGATGTAATGAAACATaag                                                                                                                                                               >  1:658050/1‑237 (MQ=255)                                                               ccccAACGGAAAAGCAAGGCAGGGTTGGGCCAAAACGCTAAACCATCACTTTGATGGGGGGACTGAGGCTGTCAATCGCCCTGACGATCCCCAGCCCCATAC‑CAAAAAAACAAAT‑CCAACCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATAATGATGATAGGGACGAAAAGGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATg                                                                                                                                                              <  2:872471/237‑1 (MQ=255)                                                                         aaaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGCTGATCGGGACGAAAACGAGACCGCCGGGGACC‑CGGATGTAATCAAACACAAAGCCCCCTTTTa                                                                                                                                                    >  2:493011/1‑239 (MQ=255)                                                                                tGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GACACTGATGATAGGGACGAAAACGGTACCCCCG‑GGGACAAGGTTGTAATCAAACAAAATGCCTCCTTTTTTTGAGtt                                                                                                                                             >  2:873002/1‑239 (MQ=255)                                                                                 gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑a                                                                                                                                           >  1:75107/1‑239 (MQ=255)                                                                                       cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAA‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AAAaga                                                                                                                                      >  1:431584/1‑238 (MQ=255)                                                                                               aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCCC‑GATCATGTGTATAGGGCCGAAAACGGTACCGCCGAGAGAC‑AGGATGGAAACAAACATAATGCCCCCCTTTTTTGAGTT‑AAAAAAGACTGGAtt                                                                                                                             >  2:258435/1‑239 (MQ=255)                                                                                               aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCGGCCTTAATCGCCTGCAGCCAGGGGATGCGGACGCTAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGGGAC‑CGGAGGTAATCAAAAATAATCCACCCTTTTATTAGGTT‑AAAAAGGCCTGGAtt                                                                                                                             >  2:820182/1‑239 (MQ=255)                                                                                                 aCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGGGTT‑AACAGAGCCTTGATttg                                                                                                                           >  1:533502/1‑239 (MQ=255)                                                                                                             caCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATTGGGTCGAAAACGGTACCGCCGAGAGAC‑AGGAGTGAATCAAACACAATGCCTCCTTTTATTTGGTT‑AACAGAGCCTTGATTTGTTGTTTTAAcgc                                                                                                               >  2:889863/1‑239 (MQ=255)                                                                                                              aCTTTGATAGGGGAACTGAGGCAGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAAGGGATGCGGAAGATAAAGCCAATCAC‑GATCATGATGATAGGGACGAAAACGATACCGCCGAGCGAC‑AGGGTGTAAACAAACATAAACCCTCCTTTTTTTCAGTT‑AAAAAAGCCTTGTTTTGTTTTTTAAAcgct                                                                                                              >  2:405417/1‑238 (MQ=255)                                                                                                               ttttGATGGGGGGCCTGAGGCTGTCGATCGCTACACATACCCCCAGCCCCATAC‑CGAAAAACCCAAC‑CCCACCCTTTACCCCGTCCTTAATCGCCGGGCGCGAGGGGATGGGAAAGAAAAGGCAAATCAC‑GATCTTGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgt                                                                                                             <  2:576696/238‑1 (MQ=255)                                                                                                               cTTTGATGGGGGGAGTGGGGCTGTCGACCCCCATGACGATCCCCAGTCCTATAC‑CGAAAAAACAAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCAAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgt                                                                                                             <  1:509038/239‑1 (MQ=255)                                                                                                               cTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGTGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCATCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAAcgcg                                                                                                              >  1:777353/1‑238 (MQ=255)                                                                                                                 gtGAAAGGGGGACGGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCGGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAATCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGcgtcg                                                                                                           <  1:672100/238‑1 (MQ=255)                                                                                                                         gggATTGAGGTTGTCGATCGCAATGACGACCACCATCCCCAACC‑CGAAAAACCCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGGGGAAGATAAGGCAAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa                                                                                                    <  1:367444/238‑1 (MQ=255)                                                                                                                         gggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa                                                                                                    >  1:482346/1‑238 (MQ=255)                                                                                                                          ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt                                                                                                   >  1:777237/1‑238 (MQ=255)                                                                                                                          ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt                                                                                                   >  1:723593/1‑238 (MQ=255)                                                                                                                          ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTCCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATACGGCCGAAAACGGTACCGCCGGGAGAC‑ACGCTGTATTCAAACATAATGCCCCCTTTTATTGCGTTAAACAG‑GCCTTCATTTGTTGTTTTAACGCGTCCTCCTTTAt                                                                                                   >  2:870925/1‑238 (MQ=255)                                                                                                                              tGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCCGGGGATGCGGAAGATAAGGCCAATCCC‑GATCATGATGATAGGGGCGAAAACGGTACCGCCGGGCAAC‑AGGCTGTAATCAAAAATAATGCCTCCTTTTTTTTAGTT‑AACAGGGCCTTGGTTTGTTGTTTTAACGCGTCGCAGTTTATCCCtt                                                                                              >  2:277499/1‑238 (MQ=255)                                                                                                                              tGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCtg                                                                                              >  1:450165/1‑239 (MQ=255)                                                                                                                                  gctgcccagcGCCATGGCAATCACCATTCCCATAC‑CGACAAACCCAAT‑CCCAACCGTTCCCCCTGCCTAAACCGCCTGCAGCCAGGGGATGCGGAAAAAAAGG‑CAATCCC‑GATCATGAGTATAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAg                                                                                          <  1:250551/229‑1 (MQ=255)                                                                                                                                       cgcacGCCATGACGACCCCCAGGCCCATAC‑CGAAAAAACCAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAggg                                                                                     <  1:621833/235‑1 (MQ=255)                                                                                                                                            gCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCGGCTTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACTGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGgcggcg                                                                                <  1:493011/239‑1 (MQ=255)                                                                                                                                                tGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGGGCAGGGCGGGGCCAt                                                                            >  1:375012/1‑239 (MQ=255)                                                                                                                                                 gccgtcacccAGTCCAATAC‑CCAAAAAACCAAT‑CCCAACCGTTCCCCCTGCCTTAATCGCTGGCAGCCAGGGGATGCGGAAGATAAGGCAAATCAC‑GATCATGATGATAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                           <  2:709410/231‑1 (MQ=255)                                                                                                                                                   agATCACCAG‑CCCCTACACGAACAAACCAAT‑CCCAACCGTTACCCCGTCCTTAATCGCCTGCAGCCAGGGGAGGCGGAATATAAGGCAAATCAC‑GAACATGATTATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                           <  1:247540/236‑1 (MQ=255)                                                                                                                                                   acatacccAGTCCCATAC‑CGAAAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCAGGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt                                                                           <  1:871915/231‑1 (MQ=255)                                                                                                                                                      tCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGCTTTGTTGTTTTAACGCGTCGTCGTTGATCCCGTTGAGCAGGGCGGCGCCATTAAGCg                                                                      >  1:823309/1‑239 (MQ=255)                                                                                                                                                           gtacccctcc‑cGACAAAAACGAT‑CCAAGCCGTTCCCCTGGTCTGAATCGGAAGCGGCAGGGGAAGGCGGAAGAAAAGGCCAATCCA‑GGTTAGGATGATAGGGCAGAAAAGGGGACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCGGTGAGCAGGGCGGCGCCATTAAGCGTAtgg                                                                 <  2:542813/230‑1 (MQ=255)                                                                                                                                                            gTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGGGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGACGGGCGGCGCCTTTAAGCGTAggaa                                                                >  1:774561/1‑237 (MQ=255)                                                                                                                                                            gTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAACGGTAgggt                                                                >  1:82851/1‑238 (MQ=255)                                                                                                                                                            gTCCCATAC‑CAACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGGGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGTTCCCTGTGAGCAGGGCGCGCCCCTTAAGCCTGGGGa                                                                >  1:704402/1‑239 (MQ=255)                                                                                                                                                             tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCTTCGTTGATCCCTGTGAGCAGGGCGGGGCCATAaagggaaggggg                                                               >  2:373074/1‑237 (MQ=255)                                                                                                                                                             tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGAt                                                               >  1:874825/1‑239 (MQ=255)                                                                                                                                                             tCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAACGGTAGGGAt                                                               >  1:654308/1‑239 (MQ=255)                                                                                                                                                             ccccATCC‑CAAAAAACCCAAA‑CCCCACCGTTACCCCCGCCTTAATGCCCTGCAGCCGGGGTATGCGAAAGAAAGGGCCAACCCG‑GTTCAGGATGAGAGGGAAGAAACGGGTACCGCCGAGAGGC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGAt                                                               <  2:874825/238‑1 (MQ=255)                                                                                                                                                              cccATAC‑CCAAAAAACCAAC‑CCCAACCGTTCCCCCTCCCTTAATCGCCTGCAGCCAGGGGAGGCGGAAGATAAGGCCAATCAC‑GATAATGATGATGGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAggg                                                                 <  1:465265/236‑1 (MQ=255)                                                                                                                                                                    c‑cGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACCGAGCCTTGATTTGTTGTTTTAACGCATCGGCGTTGATCCCTGTGCGCAGGGCGGGGCCATTAAACGTAGGGATggcaaaa                                                        >  2:256578/1‑233 (MQ=255)                                                                                                                                                                     acccaaaaaCCAATACCCACCCTATCCACCGGCCTTAATCGCCCGCGGCAGGGGGTAGGGGAAGAAAAGCCAAATCAC‑TAAAATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTa                                                         <  2:753701/234‑1 (MQ=255)                                                                                                                                                                        aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATttgtt                                                                                                                         >  1:96868/1‑171 (MQ=255)                                                                                                                                                                        aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc                                                      >  1:550341/1‑238 (MQ=255)                                                                                                                                                                        aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc                                                      >  1:457613/1‑238 (MQ=255)                                                                                                                                                                        aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGACGTCGTTGATCCCTGGGAGCAGGGCGGCGCCATTAAGCGGAGGGAGGCCGGAATCa                                                     >  2:711752/1‑239 (MQ=255)                                                                                                                                                                        aCAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCATCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTTGTCGTTGATCCCTTGGGGCAGGGGGGGGCCATTAAGGGAAGGGTGGCCGGaaac                                                      >  2:285603/1‑236 (MQ=255)                                                                                                                                                                                        aCCGTTCCCCCTGATTTAATCGCCTGCAGCAAGGGGGTGCGGAAGATAAGGCAAATCAC‑GATCAGGATGATGGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATg                                      <  1:870925/239‑1 (MQ=255)                                                                                                                                                                                        aCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGGGCAGGGGGGCGCCCTTAAGCGTAGGGAGGCCGTAACCACTTTTGGTTCGCATg                                      >  2:2586/1‑239 (MQ=255)                                                                                                                                                                                        aCCGTTACCCCGGCCTTAATCGCGTGCAGCCAGGGGATGCGGAAGATAAGGCAAATCAC‑GATCATGAAGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATg                                      <  1:523085/239‑1 (MQ=255)                                                                                                                                                                                             tcccctcgctttAATCGCTGGCGGCCAGGGGATGGGAAAGATAAGGCCATTCCG‑GATATGGGTTATAGGAACGAAAACGGAACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGt                                 <  2:421200/229‑1 (MQ=255)                                                                                                                                                                                             tcccccTGCCTAAATCGCCTGCAGCCGGGGGAGGCGGAAGAAAAGGCCAATCCC‑GATCATGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGt                                 <  1:86769/237‑1 (MQ=255)                                                                                                                                                                                             tACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTGGATCCCTTTGTGCAGGGCGGCGCCATTAAGCGTGGGGAGGCCGAAATCCCTTTTGGTTCGCAGGGAGGt                                 >  2:523085/1‑239 (MQ=255)                                                                                                                                                                                               ccccTGCCTTAATCGCCTGCGGCCAGGGGATGGGGAAGATAAGGCAAATCAC‑GATCAGGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTa                               <  1:314340/239‑1 (MQ=255)                                                                                                                                                                                                   tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGCGCAGGGCGGAGCCATTAAGCGTAGGGATGCCGTAATCACTTTTGGTTGTCTGGGAGGttcaaaa                           >  2:699521/1‑234 (MQ=255)                                                                                                                                                                                                   gtccTTAAGCCCCTGCAGCCAGGGGAGGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt                           <  1:873002/237‑1 (MQ=255)                                                                                                                                                                                                   gcgcTTACTCCCCTGCTGCCGGGGGGTGCGGAAAAAAGGGCCAATCAC‑GATCATGATGATGGGGACGAAAGCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATGTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt                           <  2:631181/236‑1 (MQ=255)                                                                                                                                                                                                    gCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCCCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTataata                          >  1:136310/1‑239 (MQ=255)                                                                                                                                                                                                    cccTTAAGCCCCGGCGGCCGGGGGAGGGGAAAGAAGAGGCCAACCAC‑GATCATTATGATGTGGAAAAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATa                          <  2:777237/238‑1 (MQ=255)                                                                                                                                                                                                      ctaaaTCGCCTGCAGCCAGGGGATGCGGAAGAAAAGGCCAATCAC‑GATCATGATGATAGGGACAAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAAt                                                       <  1:537448/205‑1 (MQ=255)                                                                                                                                                                                                          aTCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCCCTGTTGGTTCGCATGGAGGTTACAATAAGGtac                    >  1:631181/1‑237 (MQ=255)                                                                                                                                                                                                          aTCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACGGTGGGTTCGCATGGGGGTTAAAAAAAGGTc                     >  2:314340/1‑238 (MQ=255)                                                                                                                                                                                                           tCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GTTCATGAAGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCa                   <  1:285603/239‑1 (MQ=255)                                                                                                                                                                                                               cTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTTAGCAGGGCGGCGCCATTAAGCGTAGGGAGGCCGTAATCACTGTTGGGTCCGATGGAGGTTACAAAAAGGGCCAAAcc               >  2:580958/1‑239 (MQ=255)                                                                                                                                                                                                               cTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGGGGCGCCATTAAGCGTAGGGATGCCGTAATCCCTGTTGGTTGGCGTGGAGGTTAAAAAAAGGCCCAAAcg               >  2:437569/1‑238 (MQ=255)                                                                                                                                                                                                                tGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGGGGTTACAATAAGGTCCATACCg              >  1:270695/1‑239 (MQ=255)                                                                                                                                                                                                                 gctgCCAGGGTATTCGGAAGTTAAGGCCAA‑CACACATCATGTTGAGAGGGCACAAAATGGTACCGCCGAGATAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt             <  1:266568/236‑1 (MQ=255)                                                                                                                                                                                                                  cccccAGGGGATGCGGAATATAAGGCCAAACAC‑GATCAGTGTGATAGGAACGAAAGCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTATAATAAGGTCCATACCGtt            <  2:136310/236‑1 (MQ=255)                                                                                                                                                                                                                  caggcaggGGATGGGAAAAGTAGGGCAAACAAG‑GATTATGATGTGGGGGACGAAAGAGGTTCCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt             <  2:326092/238‑1 (MQ=255)                                                                                                                                                                                                                  cagcagggggATGCGAAAGTTAAGACCAATCAC‑GATAATGTGGATAGGGACGAAAGCGTTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGt             <  1:697912/232‑1 (MQ=255)                                                                                                                                                                                                                    gcagggggAAGCGAAAGAAAAGGCCAATCAC‑GATCAGGATGAAAGGGACGAAACCGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTa           <  1:91695/234‑1 (MQ=255)                                                                                                                                                                                                                    gCCAGGGGATGGGGAAGAAAAGGCCAATCAC‑GATTAGGATGATAGGGACGAAAACGGTACCGGCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTAc          <  1:580958/239‑1 (MQ=255)                                                                                                                                                                                                                         gggATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACTCGTCGTCGTTGATCCCTGTGAGCGGGGCGGCGGCATTAAGCGTAGGGATGCCGTAGTCACCTTTGGTTCGCATGGAGGTTCAAATAAGGACCATACCGTAACaataa     >  2:484667/1‑235 (MQ=255)                                                                                                                                                                                                                            atacGGAAGATAAGGCCAATCCC‑GATCATGATGATCGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTACTCAAACATAATGCCGCCTTTTATTGAGTT‑AACACAGCCTTGATTTGTTGTTTTAACGCGTCGCCGTTGATCCCTGTGAGCAGGGCGGAGCCATTAAGCGTAGGGATGCCGTAACCACTGTAGGTTCGCATGGCGGGTACAATAGGGCCCTAACAGTAACAGTTaaac  >  2:142381/4‑237 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        AATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC‑CGACAAAACCAAT‑CCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAC‑GATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGAC‑AGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTT‑AACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGA  >  NC_000913/4529947‑4530402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑