| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,849,865 | T→G | 19.9% | intergenic (‑16/+109) | hycB ← / ← hycA | hydrogenase 3, Fe‑S subunit/regulator of the transcriptional regulator FhlA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,849,865 | 0 | T | G | 19.9% | 157.6 / 5.0 | 84 | intergenic (‑16/+109) | hycB/hycA | hydrogenase 3, Fe‑S subunit/regulator of the transcriptional regulator FhlA |
| Reads supporting (aligned to +/- strand): ref base T (37/26); new base G (17/4); total (54/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGGTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGA > NC_000913/2849640‑2850091 | ggtggTGATGGCTTTAACCGGGGCGACCCCCGGCGC‑GGGTGTATCTTCCCAGGTGTGGCAGCGCTTCGGCGCTGATTCTTTTTCATTCAGCATCCCCCGCAGGGGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGa < 2:469270/225‑1 (MQ=255) gTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGt > 1:129758/1‑228 (MQ=255) ggCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAAc > 1:225627/1‑228 (MQ=255) ggCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGG‑GGC‑AGCCGATACAGAGCGTGGAGGCAGCAATTACAAAACGATTACACAGGCATTCCCCAGGGGAATGTCCTTTTTGACGaaaaa > 2:48850/1‑227 (MQ=255) gCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTAATTTTTGACGAAAACa > 1:649866/1‑228 (MQ=255) c‑gcgCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCTCTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACACGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCTCCCGGCATTCCTCAGGGAATTGTCATTTTTGACGCAAACACGCCGATGa > 1:523899/1‑228 (MQ=255) c‑gcgCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGCGCGTGGAGTCCGCAATTACAAAACGATTCACCAGGCATTCCTCCGGGGATTGTCCTTTTTTACGAAAACATGCCGATg > 2:237758/1‑227 (MQ=255) gcgcAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGACCGTGGAGTCGGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTTACGAAAACAAGCCggagaa > 2:418407/1‑223 (MQ=255) aGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTAAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGCCGAAAACATGCCGATGAAATga > 1:619175/1‑227 (MQ=255) aGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGT‑GGC‑AGCCGGTACAGAGCGCGGAGTCAGCAATTACAAAACGATTCACCCGGCATTCCCCGAGGATTTGGAATTTTTGGCAAAAAAATACCGAAGaaagt > 2:66340/1‑225 (MQ=255) ggTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGGCATTTTTGACGAAAACATGCCGATGAAATGTc > 1:496492/1‑227 (MQ=255) tGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTTACGAAAACCTGCCGATGAAATGTCAt > 1:192261/1‑227 (MQ=255) ctgcacaGTGGTGCCAGGGCGGGGGCGAGGATTCTTTTTCATTCAGACTCCCTCTCGGGCGCGGCATTGATTGCCGGGCGGGCGGGCGAGGCGTCTCTGACAAGGCGGCCTCAAAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGa < 2:473586/225‑1 (MQ=255) cTTCACAGGGGGGACAGAGCTGCGGGGCAGATTCTTTTTCATTCAGCATCCCTCTCAGGCGCGGCATTGATTGGAGGCCGTGCTGGGGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGa < 1:180115/228‑1 (MQ=255) cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACCGAGCGGGAAGTCAGCAATTACCAAACGATTCAACAGGCCTTCCGCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCgcaac > 2:426679/1‑224 (MQ=255) cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTACTCAGTGGATTGTCATTTTTGACGAAAACATTCCGATGAAATTCAATTTTGGaca > 1:270889/1‑227 (MQ=255) cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTAAGCAATTACAAAACGATTCACCAGGCATCCCTCCGGTGATTGTCAATTTTGACGAAAACAATCCCCAAAAACGTCATTTTCGacc > 2:162813/1‑226 (MQ=255) tggtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCac > 1:557230/1‑226 (MQ=255) ggtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCCCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGT‑GGC‑AGCCGATACAGAGCGTGGGGTCAGCAATTACAAAACGATTCACCAGGCATCCCTCAGGTGATTGTCCTTTTTAACGAAAACATGCCGATGAAATGTCAATTTCGCCCCTCATc > 1:395242/1‑227 (MQ=255) gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGAATGTCATTTTTTACGAAAACATGCCGATGAAAAGTCATTTTCGAAACACATCg > 2:548944/1‑227 (MQ=255) gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGATGCAAACATGCCGATGAAATGTCATTTTCGACACTCATcc > 1:499117/1‑226 (MQ=255) gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGG‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGCTTACCCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAAAATCCCGAAGAAATGTCATTTTCGAAACTCATcc > 2:600090/1‑226 (MQ=255) tgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAAAATGCCGATGAAATGTCATTTTCGGCACCCATCGac > 1:292688/1‑228 (MQ=255) ggaagagCTGCGGCGCAGATTCTTTTTCATTCAGCATCCCTCGCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGaca < 1:98828/225‑1 (MQ=255) aCAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCCCAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGCAACTCATCGaaac > 2:234472/1‑228 (MQ=255) ggcgtGCGGCGCAAGTTCTTTTTCATTAGGCCGCACCCGCCGGCGGGGCATTGATTGCAGGCTGTGCTGGCGATTCGTCTCTGAACAGGCGGCCTCACAAGTGG‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGACATTTTCGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGcc < 1:187568/224‑1 (MQ=255) tGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGCCATTCCTCAGGTGATTGTCATTTTTGACGAACACATGCCGATGAAATGTCATTTTAGACCCTCATCGACACCCCCCTc > 1:561100/1‑228 (MQ=255) cggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACCGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTAATTTTCAACACTCATCGCcacccccacccc > 2:625051/1‑228 (MQ=255) cggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTAACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATccc > 1:264649/1‑228 (MQ=255) ggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAACATCCCGATGAAATGTCATTTTCGACACCCATCGACacccccaccccc > 2:445743/1‑228 (MQ=255) ggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGGCATTTTTTACGAAAACATGCCCATGAAATGTCCTTTTCGACACCAATCGAAACCCCCAAcccc > 2:98828/1‑228 (MQ=255) aTTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGGAAGGTCATTTTCACCACCCAACGAAACGCCCACCCCCAAACaag > 2:232664/1‑226 (MQ=255) catccacttcAGGCGGGGCCTTGATTGCGGGCCGTGCTGGCGATGCGGCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCaaaa < 1:442192/220‑1 (MQ=255) tcacAGGGGCGGCATTGATTGCAGGCCGTGCGGGCGAAGCGTATCTGAAACGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCa < 1:627734/225‑1 (MQ=255) cctcAGGCGCGTCATTGATGCAAGGCGGGGCTGGCAAAGCGGCTCAGAACAGGCGGCGTAACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCa < 1:626342/227‑1 (MQ=255) ggCGGGGCATTAGTGGCAGGCCGGGGTGGCGATGGGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGGGGGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc < 2:446981/228‑1 (MQ=255) gcgcGGAATTTATTGCAGGCCGTGCTGTCGAGGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCt < 1:334497/228‑1 (MQ=255) gcgcGGAATGGAGTGCAGGCCGTGGTGGCGATGCGTCTCTGAACAGGCGGCCGCACAAGTTT‑GGA‑CGCCGATACAGAGCGGGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc < 1:607181/227‑1 (MQ=255) cgGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAg > 1:404092/1‑228 (MQ=255) cgGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGCAATTTTCGACACTCATCGACACGCCCATCCCCAAAAAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAg > 2:198107/1‑228 (MQ=255) ggCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCCAAACGGGCAAAGCCTc > 1:182341/1‑225 (MQ=255) ggCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTAAAAAACGATTCACCAGGCATTCCTCAGGGGATTGGCATTTTTGACGAAAACATGCCGATGAAATGCCTTTTTGTAAACTCCTTGACCCCCCCATCCCCAACAAGCAGCAACGCCTGCAAAACGGGAAAAACCCCaca > 2:610298/1‑226 (MQ=255) gtattgggtgCCTTGCTGGCGAGGCGTCTCGAAACGGGCGGCCTCACAAGT‑T‑GGCAAGCCGATACAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGGATTCCGCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCAtg < 2:296966/219‑1 (MQ=255) gCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTTGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAACCCCAGCCCactccgcggggc > 2:299834/1‑217 (MQ=255) gTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCCCCTGATGCCggggct > 2:312115/1‑224 (MQ=255) gggggATGGGTCCCTGAAAAGGCGCGCTCA‑AAATGTGGGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTcc < 1:584574/224‑1 (MQ=255) ttgtttcttGAACGGGGGGCCCCAAAAGTGG‑GGC‑CGCCGATACAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAc < 2:619175/220‑1 (MQ=255) gcctccGAACAGGCGGCCCAACAAGTGT‑GGC‑AGCCGATACAGGGCGTGGAGTCAGCAATTAAAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAg < 1:66340/226‑1 (MQ=255) tctcTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATTCCGATGAAATGTCATTTTCGACACTCATCGa > 2:631513/1‑145 (MQ=255) ctctGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGGGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGac < 1:631513/145‑1 (MQ=255) tctGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGGGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGCCGAAAACATGCCGCTGAAATGTCCTTTTCGACACTCATCGACACGCCAAGCCCCAAACCGCCGTAACACCTGCAAAACCGGCAAACCCTCACCCCCTCCTGCCGGGCTTTTTCCCCTTCCCAGtt > 2:434340/1‑227 (MQ=255) tctGAACAGGCGGCCTAACAAGTGG‑CGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGAGTGTCATTTTTGAAGAAAACATGCCGATGAAATGTCATCTTCACCACTCATCGAACGGCCAAACCCCAACAAGGCTTAATCCCGGCACAACGGGCAAAGCCTCAGTCAATCATTGCGGGATTTTTCCCTTTAcccatt > 2:184721/1‑223 (MQ=255) aaCAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCACCACGCCCATCCCCAAACAGGCGTACAGCCTGCAAAACGGGCAAAGCCTCAGCTCATCCTGCCGGCCTTGTTCCCTTTACCAGTTGGCtt > 1:174365/1‑228 (MQ=255) gcggcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCCAACAGGCGTAACCCCTGCCACACGGGCAAATCCTCAGCTCATGCTGCCCGGCTTTGTCCCTTTCCCAgacgtctcttat > 1:161122/1‑216 (MQ=255) gcggcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAACCTCAGCCCATGCTGCCGGGCTTGTTCCCTTTACCCGTTGGCTTAAAtt > 2:171136/1‑228 (MQ=255) gcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGCTGAAATGTCATTTTCGACACTCATCGACACCCCCATCCCCAAACAGGCGTAAACCCTGCACAACGGGCCAAGCCTCCGCTCATGCTGCCGGGCTTTAGCCCTTTACCATTTGCCTTAAAATccc > 2:380100/1‑226 (MQ=255) gcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCTACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGATGGCTTAAATTCAc > 2:324197/1‑228 (MQ=255) cacaAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGCCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGc > 1:153810/1‑228 (MQ=255) aaggggggC‑AGCCGATTCAGGGCGTGGAGTCAGCAATTACAAAACGATTCCCCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGcaca < 1:445743/222‑1 (MQ=255) gttgggC‑AACCGATACCGCGGGGGGAGTCAGCAATTACAAAACGATCCCCCAGGCATTCCTCAGGTGATGGCCATTTTTGACCAAAACATGCCGATGAAATGTCATGTTCGACAACCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACAt < 2:371581/223‑1 (MQ=255) gggggC‑AGTCGATGCAGATGGGGGAGTCAGAAATTACAAATCCATTCCCCAGGAATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACAtt < 1:560838/224‑1 (MQ=255) t‑gGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCCCATTGtt > 1:77265/1‑228 (MQ=255) gC‑AGCCGATACAGAGCGTGGAGTCAGAAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTATTCTAAACACCCATCCCCAACCAGCCGAACAGCAGGCAAAACGGCTAAAACCCACGCCCACGATACCGGGCTTTTTCCCTTTACAAGTTGCTTTAAATTCACCGTCACATGGtttc > 2:126357/1‑227 (MQ=255) gatacgtggCGTGTAGTCAGCAATTACAAAACGATTCCCCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg < 1:242914/220‑1 (MQ=255) tccAGAGCGTGTGGTGAGCAATTACAAAACGATTCGCCGGGCATGCCTCAGGTGATTTTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCAGCGACACGCCCATCCCCAAACAGGCTTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCg < 2:404092/226‑1 (MQ=255) tataaggcttGGAGTCAGAAATTAAAAAACGATTCCCCAGGCTTTCCTCGGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg < 2:192261/217‑1 (MQ=255) tACTGAGCGGGGAGTCAACAATTACAAAACGATTCACCCGGCATTCCTCAGGTTATTGTCATTTTTGACGAAAACATGCCGATGAAATGGCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc < 2:129758/227‑1 (MQ=255) tACAGGGTGTGGATTCCGCAATTACAAACAGATTCCCCAGGCATTCCTCAGGGTATTGTCATTTTTGACGAAAACATGCCGATGAACTGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg < 1:312115/226‑1 (MQ=255) tACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGTTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc < 1:234472/227‑1 (MQ=255) cGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGtata > 2:179395/1‑228 (MQ=255) cGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGtat > 1:477526/1‑227 (MQ=255) tGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGc > 1:223967/1‑146 (MQ=255) tGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCtttt > 1:544241/1‑169 (MQ=255) ggAGTCAGGAATTACAAAACGTTCCCCCGGGCTTTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAg < 1:162813/227‑1 (MQ=255) ggAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACCTTGTTTTCAATAGCGGTGTATAAg > 1:241865/1‑227 (MQ=255) ggAGTCAGCAATTACAAAACGATTCACCAGGCATTCCCCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGt < 2:544241/170‑1 (MQ=255) cAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCATTAGCGGCGTCTAAACTGTCGTAAACGCCa > 1:100108/1‑228 (MQ=255) ttCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTTTAAGCTGTCGTAAACGCCAGCGAtttt > 1:56721/1‑228 (MQ=255) ttCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACCCCAGCGAattt > 2:39689/1‑228 (MQ=255) ggCATTCCCCAGGTTATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCgaga < 1:548944/228‑1 (MQ=255) aTTCCTCAGGGTATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAg < 1:179395/228‑1 (MQ=255) gtCCTCAGGGTGTTGACATTTTGGACGAAAACATGCCGATGAAATGGCATTTTCGACCCTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAgt < 1:380100/227‑1 (MQ=255) cTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCCGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAATGtt > 2:531625/1‑228 (MQ=255) gTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGCTTTTTTCGAGATAGGGTTcccaa > 2:358374/1‑225 (MQ=255) | GCGGGTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGA > NC_000913/2849640‑2850091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |