Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 191,662 A→G 9.4% E269G (GAG→GGG)  tsf → translation elongation factor EF‑Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913191,6620AG 9.4% 250.0 / 2.5 94E269G (GAG→GGG) tsftranslation elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (40/44);  new base G (7/3);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAT  >  NC_000913/191441‑191889
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   
ccAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGGTGCTGAAAGAGCAAAACGCTGAGGTGACTGGCTTACTCCCCTTCGGAGTGGGTGAAGGACTAGAGAAAATTGGgactaa                                                                                                                                                                                                                               >  2:294124/1‑226 (MQ=255)
   ggTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGAGACTGGCTCTATCCGCTTCCAAAGGGGGGAAGGCATCGAGAAAGTTAGAACTGACtt                                                                                                                                                                                                                            >  2:93182/1‑228 (MQ=255)
    gTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGCGAACGTTGAgactgcattt                                                                                                                                                                                                                           >  1:560266/1‑223 (MQ=255)
      aCAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGGGAAGGCATCGAGAAAGTTGAGACTGACTTTgc                                                                                                                                                                                                                         >  1:573380/1‑228 (MQ=255)
          cTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGGGACTGACTTTgcagca                                                                                                                                                                                                                     >  1:611298/1‑228 (MQ=255)
              attccggggtGCAGTAGGGTAAGCCGAAAGAAGTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAg                                                                                                                                                                                                                 <  2:179844/219‑1 (MQ=255)
              atatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGACGTTTCTCTGACCGGTCAGCCGTTCGTCATGGAACCAAGCAAAACTATGACGCAGCTGCTGAAAGAGCGTAACGCTGAAGTGACTGCCTCCACCCGCTTCGAGATGTGTGAAGGCCTCGCGAAAGTTGAAACTGCCttgccaacaaaag                                                                                                                                                                                                                 >  2:539388/1‑217 (MQ=255)
              atatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAACGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGGGACTAACTTTGCAGACGAAg                                                                                                                                                                                                                 >  2:323370/1‑228 (MQ=255)
               tatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGGGACTGGCTTCATCCGCTTCGAAGGAGGGTAAGGCATCGAGAAAATTTAGAATGACTTTTGAGCACAAg                                                                                                                                                                                                                 >  2:285121/1‑227 (MQ=255)
               tatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGGAACTGGCTTCATCCGCTTCGAAGTGGGGGAAGGCCTCTGGAAAGTTGAGACTGACTTTCCAGCAAAAGt                                                                                                                                                                                                                >  2:89630/1‑228 (MQ=255)
                    cgATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGGGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgctg                                                                                                                                                                                                           >  1:475480/1‑228 (MQ=255)
                      atggcgTCTGGTAAGGCGAAAGTAACCGCAGAGAAAATGGTTGAAGGCCGGATGAAGAAATTCACCGGCGAAGTTTCTCGGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAGCTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgctgc                                                                                                                                                                                                          <  1:66400/222‑1 (MQ=255)
                      aagCAGTCCGGTAAGCCGAAAGAAATCGCAGAGAAAATGGGTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTGCTCTGACCGGTCAGCCGTGCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTTCAGCAGAAGTTGCTGCg                                                                                                                                                                                                         <  1:394148/226‑1 (MQ=255)
                       tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTACACCGCTTCGAAGTGGGTGAAGGCCTCGAGAAAGTTGAGACTGACTTTGCAGCAGAGGTTGGTGGGa                                                                                                                                                                                                        >  2:445823/1‑228 (MQ=255)
                       tGCAGTCTGGGAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCg                                                                                                                                                                                                         <  1:420259/227‑1 (MQ=255)
                               ggTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAg                                                                                                                                                                                                <  2:609402/228‑1 (MQ=255)
                                gTAAGCCGAAAGATATCGCAGAGAAAATGGTTGAAGGTCGCATGAAGAAATTCCCCGGTGAGATTTATCTGACCGGTCAGCCGTTAGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGTATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAg                                                                                                                                                                                                <  1:511696/227‑1 (MQ=255)
                                gTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAg                                                                                                                                                                                                <  1:345821/227‑1 (MQ=255)
                                     ccGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAACGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTCTGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAATGGACTAACGCTGAAGGGACTGGCTTCAGCGCCTTCCAATGGGGGCAAGGAACGGAAAAAGTTGAGACTTACTTTGCAAACAAAGTTGCGCCGATGTCCAGGccggct                                                                                                                                                                                          >  2:395600/1‑223 (MQ=255)
                                      cGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCACAAGAAGTTGCTGCGATGTCCAAGCAGTCtt                                                                                                                                                                                         >  1:290153/1‑228 (MQ=255)
                                        aaagagaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAACTTCACCGGCGAAGTTTCTATGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTCGTAACATCCTGAAAGAGCATAACCCTGAAGTGACTGGCTTCCTCAGCTTCGAGATGGTGGAAGGCATCAGGCAAGTTGAGTCTGCCTTCGCAGACGAAGTTGCTGCGGGGTTCAACCAGGCTTaa                                                                                                                                                                                       >  2:233888/1‑228 (MQ=255)
                                        aaagaaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGGCCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGGGACTGGCGTCATCCCCTTCCAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGGGATGACCaaaccagctaaa                                                                                                                                                                                       >  2:18269/1‑218 (MQ=255)
                                         aaggaaTCGCAGAGAAACTGGTTGAAGGCCGAATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTAGGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAAt                                                                                                                                                                                      <  1:224055/228‑1 (MQ=255)
                                         aagaaaTCGGAAAGAAAATGGGTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCAGTTCGTTATGGAACCAAGCAAAACGGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGGGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAAt                                                                                                                                                                                      <  1:323370/228‑1 (MQ=255)
                                                cGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCCAAGTGGGTGAAGGCATCCGGGAAGGTGAGACTGATCTTTCAGCCGTAGTTGCTGCGATGTCCAAGCAGTCTTAATTAACaa                                                                                                                                                                                >  2:78882/1‑227 (MQ=255)
                                                    gagaAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAgg                                                                                                                                                                           <  1:408308/228‑1 (MQ=255)
                                                     agaAAATGGTTGAAGGCCGAATGAAGAAATTCACCGGCGAAGTTTCTCGGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGa                                                                                                                                                                          <  1:501961/228‑1 (MQ=255)
                                                                       gCATGAAGAAATTCACCGGCGAAGCTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGGGACTGGCTTCGTCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTTGCTTTGCAGCAGAAGTTGCTGCGATGTCAAAGGAGTATTATTTATCAAAAAGGACCCGCCCGGGgggggcat                                                                                                                                                        >  2:573953/1‑226 (MQ=255)
                                                                        cATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGGGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATAAAAAAGGGGCCGTCTAGGGGCGGgtt                                                                                                                                                        >  2:600560/1‑227 (MQ=255)
                                                                        cATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCaaaaa                                                                                                                                                                             >  1:241484/1‑206 (MQ=255)
                                                                        cATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCaaaaa                                                                                                                                                                             <  2:241484/206‑1 (MQ=255)
                                                                                 aTTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTtgtg                                                                                                                                               <  1:444979/227‑1 (MQ=255)
                                                                                   tCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGGCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGccc                                                                                                                                            <  2:447632/228‑1 (MQ=255)
                                                                                     aCCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGACGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGAAGTCTTAATTATCAAAAAGTAGCCGCCCGAGGGCGGCTTCTTTTTGTGCCCAt                                                                                                                                          >  2:550447/1‑228 (MQ=255)
                                                                                       cGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCCTCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCCAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATc                                                                                                                                         >  1:88630/1‑227 (MQ=255)
                                                                                         gCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTCACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGGAACCGCCTTTGCAGCAGAGGTTGCTGCGATGTCCACGCAGTCTTCTTTATCAAAAAGGAGACCACCGAGGCCGGCTACCTTTTGTGccaactctg                                                                                                                                      >  2:64255/1‑221 (MQ=255)
                                                                                          cGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGt                                                                                                                                     >  1:146908/1‑228 (MQ=255)
                                                                                             attttCCCTGACGGGTCAGCCGTTCGTTATGGAACAAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTaaa                                                                                                                                  <  2:562543/226‑1 (MQ=255)
                                                                                             aGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAAGCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTaaa                                                                                                                                  <  1:600560/228‑1 (MQ=255)
                                                                                                        ccGGTCAGGCGTGCGGTGAGGAGCCAAACAAATCTGTTGGTCAGCTGCTGAAAGGGCATAACGCTGAAGTGACTGGCTGCATCCGCTTCGAAAGGGGTGAAGGCATCGAGAAAGGTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAAc                                                                                                                        <  1:96970/227‑1 (MQ=255)
                                                                                                           gTCAGCCGTTCGTTATGGCACCAAGCAAAACTGTTGGGCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAAccc                                                                                                                      <  1:581941/226‑1 (MQ=255)
                                                                                                                 cgttcgttATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGGTCATAACGCGGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTgggg                                                                                                              <  2:399653/228‑1 (MQ=255)
                                                                                                                 cgttcgttATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCACCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGAGGTCCAAGCAGCCTTAATTATCAAAAAGGAACCGCCTGAGGCCGGCTCCTTGTTTTGCCCATCTTGTAAATTCTGCTACCCCCTGTgggg                                                                                                              >  1:407518/1‑228 (MQ=255)
                                                                                                                 cgttcgttATGGAACCAAGCAAAACGGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTgggg                                                                                                              <  1:165760/228‑1 (MQ=255)
                                                                                                                 cgttcgttAAGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTgggg                                                                                                              <  1:89630/228‑1 (MQ=255)
                                                                                                                   ttcgttATGGACACAAGCAAAACTGTTGGGCAGCTGCTGAACGTGCATAACGCTGAAGTCACTGGCTTCATCAGCTTCGAAGTGGGTCAAGGCATCGAGAAAGTTGAGACTGACTTTGTAGCAGAAGTTGCTGAGATGTCCAAACAGTCTTAATTATCAAAAAGGAGCCGCCCAAGGGCGGATTATTTTTGTGCCCATCTTTTAAATTCAGCCATCCTTTGTGGGGCt                                                                                                            >  2:234596/1‑228 (MQ=255)
                                                                                                                          tGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCCTAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGCTGGTGAAGGCATCGAGAAAGTTGAGCCTGACTTTGCAGCAGAAGTTGCGGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATGTTTTAAATTCGGCTAACCCTTTTgggggggggcgga                                                                                                     >  2:434387/1‑219 (MQ=255)
                                                                                                                          tGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCAATCTTGTAAATTAAGATAACCCTTACGGGGGAGCCCTGa                                                                                                     >  2:246680/1‑228 (MQ=255)
                                                                                                                          tGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGGTCCCATCTTGTAAATTAAGCTAACCCTTGTGGGGCTGCGCTGa                                                                                                     >  2:92499/1‑228 (MQ=255)
                                                                                                                                cacgCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCAGCTTCGAAGGGGGTGAAGGCATCGAGAAAGTTGGGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTATTTATCATAAAGGAGCCGCCTGCGCGCGGATTCTTTTTGTGCCCATCTTATAACTTACGCTAACCCTTGTGGGGTTTCGCTGCAAAGCg                                                                                               >  1:597310/4‑228 (MQ=255)
                                                                                                                                cAAGCAAATCTGTTGGGTGTGTGCAGAAAGAGAAGAACGTTGAAGTGAATGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTAGGACTGACTTTGCAGCTGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCg                                                                                               <  1:294124/228‑1 (MQ=255)
                                                                                                                                cAACCAAACTTGTTGGTCGCATGCTGAAAGACAATAACTCTGCAGTGACGTTGTTCATCCGGTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGATTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCAGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCg                                                                                               <  2:606808/228‑1 (MQ=255)
                                                                                                                                  aGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGCCTTTGCAGCAGAAGTTGCTGAGTTGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGCGGGCGGCTTCTTTTTGGGACCTTCTTGTCAATTAAGCTAACCCTTGGGGGGCTGCGCTGaaaaaggac                                                                                             >  2:184453/1‑223 (MQ=255)
                                                                                                                                  aGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCgc                                                                                              >  1:132997/1‑226 (MQ=255)
                                                                                                                                  aGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGa                                                                                              >  1:47505/1‑227 (MQ=255)
                                                                                                                                  aGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCACCTAACCCTTGTGGGGCTGCGCGGAAAAACgca                                                                                             >  2:382624/1‑226 (MQ=255)
                                                                                                                                       aaCTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGCCTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGCATTAATTATCAAAAAGTATCCCCCTGAGGGCGGCTTCTTTTTCGGCCCCTCTTGTACATTCAGCTAACCCTTGTGGGCGTGCGCTGAAAAACCACGtcaa                                                                                        >  2:388484/1‑225 (MQ=255)
                                                                                                                                               gTCAGCTGATGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCt                                                                                <  1:23206/228‑1 (MQ=255)
                                                                                                                                                tCAGCTGCTGAAAGGGCATAACGCTGAAGTGGCTGGCTTTATCCGGTTCGAAGTGGGTGAAGGACTCGAGAAAGTTGAGACTGACTTTGCAGGAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCg                                                                                  <  2:630582/225‑1 (MQ=255)
                                                                                                                                                tCAGCTGCTGAAAGGGCATAACGCTGAAGTGCCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGc                                                                                 <  1:372989/226‑1 (MQ=255)
                                                                                                                                                       ctgAAAGGGCATAACGCTGAAGTGACTGGCTTCATCCGCTGCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGCCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGTCGGCTTCTTCTTGTGCGCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTaa                                                                        <  1:78882/228‑1 (MQ=255)
                                                                                                                                                       ctgAAAGGGCAAAACGCTGAAGTGACGGGCTTCAACCGCTTCGAAGGGGGGGAAGGCAGCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCGGCGAGTTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTaa                                                                        <  2:560266/228‑1 (MQ=255)
                                                                                                                                                         gAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAAtt                                                                      <  1:18269/228‑1 (MQ=255)
                                                                                                                                                         gAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAAtt                                                                      <  1:603947/228‑1 (MQ=255)
                                                                                                                                                          aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAACGACGTACAATGACGCTCGTATTAATTc                                                                     >  2:406629/1‑228 (MQ=255)
                                                                                                                                                              ggcgataCGCTGAAGGGACTTGCTTCATCCGTTTGGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGGCCAAGCAGTCTTAATTAGCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAttt                                                                 <  1:539388/222‑1 (MQ=255)
                                                                                                                                                              agCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAttt                                                                 >  1:173448/1‑228 (MQ=255)
                                                                                                                                                                    aCGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGc                                                                                 >  2:226623/1‑206 (MQ=255)
                                                                                                                                                                    aCGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTAAATCg                                                           >  1:129599/1‑228 (MQ=255)
                                                                                                                                                                     cGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCt                                                                                <  1:226623/206‑1 (MQ=255)
                                                                                                                                                                        tGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATTTCGCTAGTATTAATTCATTTCAATCGttac                                                       >  2:658587/1‑226 (MQ=255)
                                                                                                                                                                        tGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGGCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGCCTTCTTTTTGTTCCCCTCTTGTAAATTCAGCTAACCCTGGTGGGGCTGCGCTGAAAACCGCCGTCCATTGCCGCTAGATTTAATTAATTTCAATCGTTgc                                                       >  1:354987/1‑227 (MQ=255)
                                                                                                                                                                          aaggtgttGGTTTTATCCGTTTCGAAGTGGGGGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGAc                                                      <  2:573380/220‑1 (MQ=255)
                                                                                                                                                                          aaGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAACAGGGCCGGACCATGTCCCAAGGATTAATTCATTTAAACCATTgcaa                                                     >  2:376042/1‑225 (MQ=255)
                                                                                                                                                                               acgggCTTCATCCGGTTCGAAGTGGGGGAAGGGATCGAGAAAGTTGAGACTGGCTTTGCAGGAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                <  2:88630/225‑1 (MQ=255)
                                                                                                                                                                               aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGACAAGCGACGTACAATGTCGCTAGAATTAATTCATTTCAATCGTTGACAGtctc                                                >  1:560254/1‑228 (MQ=255)
                                                                                                                                                                               aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                <  1:382624/228‑1 (MQ=255)
                                                                                                                                                                               aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTAAGCTAACCCTTGTGGGGCTGAGCTGAAACGCGCCGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                >  1:640604/1‑228 (MQ=255)
                                                                                                                                                                               aCTGGCTTCAGCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                <  1:93182/228‑1 (MQ=255)
                                                                                                                                                                                cTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCa                                               <  1:406629/228‑1 (MQ=255)
                                                                                                                                                                                   gattCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGTGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGga                                            <  1:554129/226‑1 (MQ=255)
                                                                                                                                                                                   gCTTCATCCGCTTCGAAGGGGGGTAAGGCAACGAGAAAGTTGGGACGGAATTTGCAGCGGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGga                                            <  2:290153/228‑1 (MQ=255)
                                                                                                                                                                                       cATCCGCTTCGAAGTGGGTGAAGGCATCGAGACAGTTGAGACTGTCTTTGCAGCAGAGGTTGCTGCGATGTCCAAGTAGTCTTAATTATCAAAAAGGAGCTGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTAAGCTAACCCTTGTGGGGCTGCGCTGAAAAGAGCCGTCCAATGTCGCTGTTATTAATTCATTTCAATCGTTGCCAGCCTCAGgaaag                                         >  1:533978/1‑227 (MQ=255)
                                                                                                                                                                                         tCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGTCGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaaa                                      >  1:534319/1‑228 (MQ=255)
                                                                                                                                                                                             cTTTGAAGTGGGTGAATGGATCGAAAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATg                                  <  1:569549/228‑1 (MQ=255)
                                                                                                                                                                                             cTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaaaaaag                                  >  2:142247/1‑224 (MQ=255)
                                                                                                                                                                                             cTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAAAAACATg                                  >  1:497404/1‑228 (MQ=255)
                                                                                                                                                                                             cTTCGAAGGGGGTGAAGGAAGCGAGGAATTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCGTAATTAACAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATg                                  <  1:234596/228‑1 (MQ=255)
                                                                                                                                                                                                  aaGTGGGTTAAGGCATCGAGGAAGGTGAGACTGGCTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc                            <  1:445823/228‑1 (MQ=255)
                                                                                                                                                                                                  aaGTGGGTTAAGGCAACCAGATAGTTTAGTCTGACTTTGCAGCAGAAGTTGCTGCGATGTCCTAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCAACTAGTAAATTCAGCTAACCCTTGTGGGGCTGCCCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAAACGTTGACAGTCTCAGGAAAGAAACATGGCTAc                             <  1:395600/228‑1 (MQ=255)
                                                                                                                                                                                                       ggTGAAGGCATCGAGAAAGGTGAGACTGACTTTGCAGCAGAAGGTGCTTCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATg                        <  1:202720/228‑1 (MQ=255)
                                                                                                                                                                                                              gCATGGAGAAAGTTGAGACTGACTTTGCAGTAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTACCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAc                  <  1:246680/227‑1 (MQ=255)
                                                                                                                                                                                                              gCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAAGTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAc                  <  1:595890/227‑1 (MQ=255)
                                                                                                                                                                                                                 tCGAAAAAGTTGAGACGGCCTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAccc                <  1:154661/226‑1 (MQ=255)
                                                                                                                                                                                                                             agaCTGACTTTGCAGCAGAAGTGGCTGCGAGTTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAt  <  1:285121/228‑1 (MQ=255)
                                                                                                                                                                                                                             agaCTGAATTTGCAGCAGAAGTTGGTGCGATGTCCAAGCATGCTTAATTATCAAAAAGGAGCCGCCGGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAt  <  2:560254/228‑1 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   
CCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAT  >  NC_000913/191441‑191889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: