Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,346,885 C→T 6.1% P103L (CCG→CTG)  ptsN → sugar‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,346,8850CT 6.1% 188.8 / 3.7 69P103L (CCG→CTG) ptsNsugar‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base C (27/36);  new base T (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA  >  NC_000913/3346660‑3347108
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               
aaaaaCGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCgg                                                                                                                                                                                                                               <  1:121794/228‑1 (MQ=255)
      gcgcCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGAGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGACGATGGCGTTTTCGTTCAGATGGAAACGACTATAGCTTTCGATGCCCACGAAAACCAACCGGTggca                                                                                                                                                                                                                          >  1:303027/1‑225 (MQ=255)
                    atcaGCGAGGCGGGGGCGAAACAACGTAGCCTGCCTGCTCAGGTGGTTTTGGAAGCTATCCTGACGCGGGAAAAAAGTGGCAGGACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCctg                                                                                                                                                                                                           <  1:39660/228‑1 (MQ=255)
                    atcaGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGCCTTACTTTTTGCCctt                                                                                                                                                                                                           >  2:172561/1‑227 (MQ=255)
                              gggcggcGAAAAAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCgg                                                                                                                                                                                                 <  1:419443/227‑1 (MQ=255)
                                      aaaCACTTTGGCCTGCGGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCa                                                                                                                                                                                          <  1:9338/227‑1 (MQ=255)
                                               aGCCGGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTCTTGCCATTCCGACTGGCCCACTGGAAGCCGCTCCTCTGCGCGCCGTTGGCGGTTTCGTTCAGCTCGAACAGCCTCTAGCTTTCGCTGCCATGGACACCCCCCCGGTGGAATTCCTTTTTGCCCTGCCGGTGCCGGCCGACAAAACCaaaaa                                                                                                                                                                                 >  2:45111/1‑226 (MQ=255)
                                                gcctgcctCCTCATGTGGTTTTTGACGCTAGCCTGACGCGGGAAAAAATGGGCAGTACCGGTTCCGGCAATGGTATTGCCGTTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg                                                                                                                                                                                <  2:587286/227‑1 (MQ=255)
                                                gCCTGCGGCCGTGGGTGGTTGTTGAAGCTATCTTGACACGGGAAAAAATGGGAAGTACCGTTCTCGGCAAGGGTATTGCGATTCCGCATGGCAAACTGGAAGAAGATACGCGGCGCGCCGTGGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTGCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg                                                                                                                                                                                <  2:141973/227‑1 (MQ=255)
                                                 ccTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCCAAACGCCTATAGCTTTCGATGCCACCGACAACCAACAGGCGGACTTCATTTTTGCCCTGCTGGTGCCGGCAGACCAACCTaaaaaacc                                                                                                                                                                              >  2:609936/1‑224 (MQ=255)
                                                 ccTGCCGCCTCAGGGGGTTTTTGAAGCTTTCCTGGCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa                                                                                                                                                                              <  2:489957/228‑1 (MQ=255)
                                                 ccTGCCGCCTCAGGGGGTTTTCGAAGTCTGCCGTACCCGGGAAAAAATGGTCAGCACCGGGATCGGCAATGGTAGTGCTATTCCGCATGGCAAACTGGAAGAAGATATCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa                                                                                                                                                                              <  2:351083/228‑1 (MQ=255)
                                                 ccTGCCCGCTAAGGTGGTTTGTGAAGTATACCTGACGGGGGAAAAAATGGGAAGTACCGGTATCGCCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa                                                                                                                                                                              <  2:551141/228‑1 (MQ=255)
                                                  cTGCCGGCTCAGGTGGTTTTTGAAGCGATCCTGACGCGGGAAAAAATGGGCAGTACCGGTAACGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa                                                                                                                                                                              <  1:344917/227‑1 (MQ=255)
                                                      cgccTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTg                                                                                                                                                                          >  2:658047/1‑227 (MQ=255)
                                                       gcctgcggtggtTTTTGAAGCTATACCGCCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAAGGGTATTGCCATTCCGAATGGCAAACTGGAAGAAGATTCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCa                                                                                                                                                                        <  2:660023/222‑1 (MQ=255)
                                                        tcTCAGGTGGTTTTTGAAGGTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAt                                                                                                                                                                       <  1:239399/227‑1 (MQ=255)
                                                                   ttttGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGGCAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCt                                                                                                                                                            >  1:268866/1‑228 (MQ=255)
                                                                            tATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACAAAACTAAAACGCACCTCCTTACCCTGTCCCtgtgggccaa                                                                                                                                                   >  2:87939/1‑220 (MQ=255)
                                                                                    gcgggggAAAAAATGGGCAGTACCTGTAGCGGGATTGTTATTGCCGTTCGGCAGTGCAAACTGGAAGAAGATGCCCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg                                                                                                                                            <  2:551580/224‑1 (MQ=255)
                                                                                         tgAAAAAATGGGCAGGCCCGGTATCGGCAATGGTAGTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTTCGCGCCGTTGGCGTTTCCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGa                                                                                                                                       <  2:169646/227‑1 (MQ=255)
                                                                                         ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGCTACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCAGGTGTCGAAACGGCTGGCgg                                                                                                                                        >  2:347388/1‑227 (MQ=255)
                                                                                         ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTAGCAACGCCTATAGCTTTCGATGCCATCGACAACCCAACGGTGGACTTACTTTTTGCCCTGCTGGTGCGGGCAGACCAAACCAAAACACACCTCGATACACTTTGGCTGGTGGCGAACAGTCTTGCgg                                                                                                                                        >  2:459644/1‑227 (MQ=255)
                                                                                                                  ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCACACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgg                                                                                                              >  1:651485/1‑227 (MQ=255)
                                                                                                                  ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGTTGGTGGCGAAACGTCTGGCGGACAAAACCACCTGCCCCCGTTTAcgc                                                                                                              >  2:561255/1‑228 (MQ=255)
                                                                                                                  ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCCTCATACTCTGTCGCTGGTGGCGCAAGGTCTGGCGGACAAAACAATCTGCCGCCGTTTAcgc                                                                                                              >  2:230422/1‑228 (MQ=255)
                                                                                                                   gCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAACTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg                                                                                                             >  2:257766/1‑228 (MQ=255)
                                                                                                                    gtaTGGTTTTGCCTTGCCGCAAGGAAAACTGGAAGGAGATTTGCGGCGAGGCGTTGGCGGTTTCGTTCAGCCCGAAATGCCTATGGCTTTCGAGGCCATCGACAACCAACCGGTGGACGTTCGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTCAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg                                                                                                             <  2:432724/225‑1 (MQ=255)
                                                                                                                    gaaTGGTTTTGTCATTCCGCAGGGCAAACTGGAAGAAGATACTGTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg                                                                                                             <  2:226614/226‑1 (MQ=255)
                                                                                                                    cAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATCCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg                                                                                                             <  1:105185/227‑1 (MQ=255)
                                                                                                                    aaaTGGTATGGCCATTCCGCAGGGCAAACTGGAAGAAGATTCCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg                                                                                                             <  1:316976/226‑1 (MQ=255)
                                                                                                                          tttttgcATTCCGCCTGGCAAACTGGAAAAAGTTACTTTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCa                                                                                                      <  2:161629/222‑1 (MQ=255)
                                                                                                                            ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGGCTGGGGGACAAAACCATCTCCCCCCGTTTACCcccagcccagg                                                                                                    >  2:329782/1‑227 (MQ=255)
                                                                                                                            ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATATTCCGTCGCTGGTGTCGAAACGTCTGCCGGCCAAAACCATCTGCCGCCGTTTACCCGCAACCCaca                                                                                                    >  2:439536/1‑226 (MQ=255)
                                                                                                                                cATTCCGCATGGCAAACTGGAAGAAGATATCCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGGCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTTGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg                                                                                                 <  1:230422/227‑1 (MQ=255)
                                                                                                                                  ttCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg                                                                                              <  1:329782/228‑1 (MQ=255)
                                                                                                                                   tCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGCCAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGTGa                                                                                             >  1:438881/1‑228 (MQ=255)
                                                                                                                                   tCCGCAAGGCAAACTGTAAGAAGATACTCTGCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACCGGGGGACTTACTTTTTTCCCTGCTGGGGCCGCACGACCAACCTAAAACGAACCAGACTACTCTATGCCTAGTGGCGAAACACTTGACGGACAAAACAAACTGCCGCCGTTTAACGGCAGCCCAGAGCgggga                                                                                             >  2:442950/1‑224 (MQ=255)
                                                                                                                                    ccGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGGGCCACCCAGAGCGagtaa                                                                                            >  1:53028/1‑224 (MQ=255)
                                                                                                                                       gaTGGAAAACTGGAAGAAGATACGCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAATCGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAagag                                                                                         <  2:438881/227‑1 (MQ=255)
                                                                                                                                        cgggCAAAATGGAAAAAGATACCGTTCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCCTCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGc                                                                                        <  2:331376/226‑1 (MQ=255)
                                                                                                                                         tGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGAAGAGCt                                                                                       >  1:72437/1‑228 (MQ=255)
                                                                                                                                            cAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCAACTGCCGCCGTTTCCGCGACCCCCGGAGCGATAGAGAGCTGt                                                                                     >  2:402464/1‑227 (MQ=255)
                                                                                                                                                 tGGAAGAAGATACTCTGCGGGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCaaa                                                                               <  1:389554/228‑1 (MQ=255)
                                                                                                                                                   gaagagGATTCCCTGGCCGCCGTTGGCGTGTTCGTTCAGCTCGAAACTGCTAATGATTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAAt                                                                              <  1:261005/227‑1 (MQ=255)
                                                                                                                                                              tctGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTCTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGAGACGAGATGAATCAAATACTTACgagtaa                                                                  >  2:73641/1‑223 (MQ=255)
                                                                                                                                                                tGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAt                                                                                               >  2:423725/1‑197 (MQ=255)
                                                                                                                                                                 gcgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg                                                                                              <  1:423725/197‑1 (MQ=255)
                                                                                                                                                                  cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGc                                                                                                  >  1:32957/1‑192 (MQ=255)
                                                                                                                                                                  cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGTCGGACAAAACCATCTGCCGCCGTTTACGCGCCACCCCGAGCCGTGAAAAGCTGAATCAAAACATTCCCGAAACCGaa                                                              >  2:248965/1‑228 (MQ=255)
                                                                                                                                                                  cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCGGAGCGATGAAAAGCTGTATCAAATCATTACGGATACCGaa                                                              >  1:121907/1‑228 (MQ=255)
                                                                                                                                                                  cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGac                                                              >  1:549190/1‑227 (MQ=255)
                                                                                                                                                                   gcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg                                                                                                 <  2:32957/192‑1 (MQ=255)
                                                                                                                                                                        gTTGGCCTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACt                                                        <  1:55919/228‑1 (MQ=255)
                                                                                                                                                                                 ttCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACCAAACCATCTGCCGCCGTTTACCCGCAGCCCAGAGCGATGAAGAGCTGTATCCAATCATTACGGATACCGAATGGACTCCGGATaaa                                               >  2:401749/1‑228 (MQ=255)
                                                                                                                                                                                  tCGTTCAGCTCGAAACGCCTTTAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAg                                              <  2:557753/228‑1 (MQ=255)
                                                                                                                                                                                                    cTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACa                                                                                                                                     >  1:616779/1‑123 (MQ=255)
                                                                                                                                                                                                     tataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACaa                                                                                                                                    <  2:616779/123‑1 (MQ=255)
                                                                                                                                                                                                      ttaGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt                           <  1:347388/226‑1 (MQ=255)
                                                                                                                                                                                                      attgCTTTCGCTGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTTCATACTCTGCCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt                           <  1:520404/224‑1 (MQ=255)
                                                                                                                                                                                                      ataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt                           <  1:257766/227‑1 (MQ=255)
                                                                                                                                                                                                       taGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGtct                         <  1:405191/228‑1 (MQ=255)
                                                                                                                                                                                                            ttCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGAAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGAACTCCGGATGAAGCGTCGTTATTCGGTAAGGTCTCtttt                    >  1:187907/1‑228 (MQ=255)
                                                                                                                                                                                                             tCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGTTACCGCAGGGACTCCGGATGAACCGCAGTTATTCGGTAATGTATCTTTTa                   >  2:83119/1‑228 (MQ=255)
                                                                                                                                                                                                              tgATGCCCGCGACCACCAACCGGTGGCACTACGTTTTGCCCTGCTGGCGCCGGCAGACCAAACTAAAACGCACCTGCATGCGCTGTCGCTGGTGGCGAAACGTCGGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAGGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAg                  <  2:392818/227‑1 (MQ=255)
                                                                                                                                                                                                                    cATCGACAACCAACGGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGACGAGCTGTATCAAATCCTTACGGATACCGAAGGAACTCCGGAGGAAACGTAGTTTTTCGGAACTTTCTCCTTTaagctgtg            >  2:361847/1‑221 (MQ=255)
                                                                                                                                                                                                                       cGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTtgtg          <  1:73641/227‑1 (MQ=255)
                                                                                                                                                                                                                         gcaaccaacCGGTGGACTTCCTTTTTGCCCTGCTGGGGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg       <  1:432318/227‑1 (MQ=255)
                                                                                                                                                                                                                         acaacaAACCGGTTGCCTTTCTTTTGGCCCGGGTGGGGCCGGCCGGCCAAACTAAAACGCACCTGCATACTCGGGCGCTGGTGGGGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATTATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg       <  2:268866/228‑1 (MQ=255)
                                                                                                                                                                                                                              caacgGGGGGACTTTCTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa  <  2:549190/228‑1 (MQ=255)
                                                                                                                                                                                                                               aaccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa  <  1:658047/227‑1 (MQ=255)
                                                                                                                                                                                                                                accGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCt                                                                                                                                             >  1:501998/1‑87 (MQ=255)
                                                                                                                                                                                                                                 ccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg                                                                                                                                            <  2:501998/87‑1 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               
AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA  >  NC_000913/3346660‑3347108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: