| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,355,507 | G→C | 20.3% | P261P (CCG→CCC) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,355,507 | 0 | G | C | 20.3% | 51.9 / 2.8 | 37 | P261P (CCG→CCC) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/6); new base C (5/3); total (28/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01 | |||||||||||
CTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCCGGC > NC_000913/3355289‑3355734 | cTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCCATGGGCGCGCGCGCGTACTTATAAATTCCAGACACCGCTTATccc > 1:4869/1‑227 (MQ=255)cTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCCCGTACTTATAAATTCCAGACACCCCTTATCCCt > 2:56449/1‑228 (MQ=255) tgtgTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTTTTCCACCAGCGCTTCTCCACTCACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGGAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAAACACCCGTTAACCCt > 2:532745/1‑227 (MQ=255) tgtgTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTTGCGCATACCGGTGAAATCAACACCACCACCGGTAACCGCCAATGGGCGCGTGCCGGGAGTTATAAATTCCAGACACCCCTTATCCCt > 1:267184/1‑227 (MQ=255) tgtgTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTAATCCCt > 1:470015/1‑227 (MQ=255) tgtgTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGCACTTATAAATTCCAAACACCCCTTATCCCt > 2:105548/1‑227 (MQ=255) cGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGTCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCCCGTACTTATAAATTCCAGACACCGCTTAGCCCTGACCTGCccgacg > 1:259750/1‑228 (MQ=255) cGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTTCacgact > 1:372390/1‑227 (MQ=255) tGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATAAACACCATAACCGGTAACAGCCAATGGGCGCGGGCGCGTACTTATAAATTCCAGACACGGCTTATCCCTGACCTGCACCACGCCGCACCGTTCGTCAACGAAACCGGct > 1:176362/1‑227 (MQ=255) tCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGc > 1:299447/1‑182 (MQ=255) gcgcTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCACCGAAACCGGCCCTGACTCCAGTTCGATGGATAACATCTGGGAACTGCTGCTGGc > 2:618547/1‑228 (MQ=255) acacCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACCAAACCGGCTCTGACTCCCGTTCGATGGATAACTTGCTGGAACTGCTGCTGCCAGGCgggcaggaaa > 2:153818/1‑221 (MQ=255) cGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCCGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCCggatggaaaacaa > 2:23416/1‑222 (MQ=255) gTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCACCGAAACCGGCCCTGACCCCAGTTCGATGGATAACATCCTCGAACTGCGTCTCGCAGGCGGGATGGataatatc > 2:159356/1‑223 (MQ=255) gTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATatcatc > 1:462416/1‑228 (MQ=255) gTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCCCTTATCCCTGCCCTGCACGACGCCGAACCGTCCGTCACCGAAACCGGCCCTGACACCAGTTCGATGGTTAACATCCTGGAACGCCCGCCGGCAGGCGggacggagacacac > 2:92121/1‑221 (MQ=255) aCCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCCCTGCATCCAGTTCGATGGATAACAGGCTGGAAATGCTCCTGTCAGCCGggatggtaatcctc > 1:268000/1‑218 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGCCTCCAGTTCGATGGATAACATGCTGGACCTGCTGCTGCCAGGCGGGATGGATCTCATCCgcgc > 1:13910/1‑228 (MQ=255) cgctggccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGCTGGCTCACATGCTGGAACTGCTGCTGGCAGGCGGGATGGAGTTCATccccccc > 1:380210/1‑223 (MQ=255) cgctggccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACCCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGCTAACATGCTGGAACTGCTGGTGGCAGGCGGGGAGGGAATCATCCGCGcc > 2:657628/1‑228 (MQ=255) gTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGACAACATGCTGGAACTGCTGCTGCCAGCCGGGATGGATATAATCCGCGCCATGCGTCTATTAGTac‑cacc > 1:234506/1‑228 (MQ=255) cctctcgcgggCCCATGACGGAAAAAACCACCCCATCCCCGGAACCCGCCAATGGGCCCGTGCGCGTTCTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCg < 1:92121/218‑1 (MQ=255) cgcATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCCCTGACTCCAGTTCGATGGATACCATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCTTCCCGCCCCTGGGTCTTTTAGTAC‑CACCCGCCCGCCAGaaca > 2:364543/1‑228 (MQ=255) aaCGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGCAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGACCTGCTGCTGCCAGGCGGGATGGTTATCATCCGCGCCATGCGTCTCTTATTAC‑CACCCGCCTTGCATAACATCCCg > 1:585319/1‑228 (MQ=255) aaCGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGCTGGCTAACATGCTGGAACTGCTGCTGGCATGCGGGATGGAAATCATCCGCGCCACGCGTCCTTTATAAC‑CACCCCCCTGGCAGAAAAACCCg > 2:513604/1‑228 (MQ=255) tGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGCTAACATGCTGGAACTGCTGCTGGCAGGCGGGAGGGGTATCATCCCCGCCATGCGGCTATTAGTAC‑CCCCCGCCTGGCAGACCAACCCgagtag > 2:155220/1‑223 (MQ=255) acCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTCCTTATAACTTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCCCCGACTCCAGTTCGACGGCTAACATGCTGGAACTCCTGCTGGCAGACGGGATGGTTAACAGCCGCGCCATGCGCCTATTACTACACACGCCCGTGGC‑GAACAACCCGGGAATGGACCCggg > 2:26400/1‑226 (MQ=255) cGCCAGTGGGCGGGTGCGCGTACTTATAAATCCCAGACACCGCTTACCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAGACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATAGCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCttt < 2:253230/228‑1 (MQ=255) cGCCAATGGGCGCGTGCCCGGACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCttt < 1:23416/228‑1 (MQ=255) cGCCAATGGGCGCGTGCACGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCttt < 1:56449/228‑1 (MQ=255) ggcccttgcgcgtGTTTTGAAATTCCAGACACCCCTTCTCCCGGTCCTGCACGACGCCGCTCCGGTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTCTTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTaa < 2:267184/222‑1 (MQ=255) tggggttACTTAAAAATTCCAAACCCCCCTTACCCCTGGCCTGCACGAGGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCAt < 2:462416/222‑1 (MQ=255) tgcgcgtACTTATAAATTCCAGACCCCCCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCAt < 1:465544/228‑1 (MQ=255) ggcgcatACTTAAAAATTCCAGTCACCGCTGATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCAt < 1:153818/227‑1 (MQ=255) cTTATAAATTCCAGACACCGCTTACCCCTGGCCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATg < 1:136726/227‑1 (MQ=255) gACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGCCTATTAGTAC‑CACCCCCCTGTCAGAACAACCCGGATATGGGACCGGAACTGCGTGCCTTCTTTGACTTTACCTCCATGCCTATGGCGCCGTgggtggg > 2:502554/1‑224 (MQ=255) acCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTATTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGGGCCTCCTTTGCCTTTAACTCCAGCCATATGAATCCGTGGGAGTGccc > 1:551090/1‑228 (MQ=255) gCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCcggc > 1:118540/1‑228 (MQ=255) | CTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTAC‑CACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCCGGC > NC_000913/3355289‑3355734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |