Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,951,495 | T→G | 17.1% | pseudogene (936/984 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,951,495 | 0 | T | G | 17.1% | 174.0 / 3.6 | 78 | pseudogene (936/984 nt) | ilvG | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted |
Reads supporting (aligned to +/- strand): ref base T (30/31); new base G (13/3); total (44/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.55e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA > NC_000913/3951269‑3951720 | tAGAAGCAGTTTACTCGTACTGTCGGGGGATGCTGGGGATGCACGGCC‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGt < 2:290824/227‑1 (MQ=255) gATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCCCGTTTTGATGACCGGGTGACAGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAACTGAACAAGCAGCGGCATGCACATGAGGCGATACAAGGGGATATaaac > 2:249010/1‑227 (MQ=255) gATTATCCGTACTATCTGGGCATGATGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGGGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGCGTTATCCAAATGGATATCGACCCGGCAAAAATGACCAAGCTGCGTCAGGCCCATTTGGAATTACAAGGGGACTTaaaa > 2:456767/1‑227 (MQ=255) tATCCGTACTATCTGGGGATGGTGGGGA‑GCACGGGACCCACAGCGGCAAGCTTCGCGGTGCAGGGGTGTGCCCTGCTGATCGCCGTGGGCGCACGGTTTGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGct < 2:340223/228‑1 (MQ=255) cGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGGGTTATCCATATGGATATGGACCCGGCAAAAATAAACAAGCTGCTTCAGGCACACGTGGCCTTACAAGGGGAATTAAAtccccgtt > 2:473057/1‑221 (MQ=255) aCTATCTGGGGATGGTGGGGATGCACGCCA‑CCAAAGCGGCAAACTGCGCGTGGCAGGAGCTTGACCTTCTGATCGCCGTGGGCGGCCGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc < 2:41688/228‑1 (MQ=255) ttttCTGGGCCTGCTGGGGATGCACGGAA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGTCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc < 1:249010/224‑1 (MQ=255) ccaTCTGGGAGTGGTGGGGATGCACGGAC‑CCAAAGCGGCAAACGTCGCGGTGCAGGCGTGTGACCTGCTGATCGCCGGGGGCGCAAGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 1:589943/226‑1 (MQ=255) aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 1:542721/226‑1 (MQ=255) aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 1:103358/226‑1 (MQ=255) tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATAGGGATATCGACCCGGCACGACTGAACAAGCTGCGTCAGGCCCATGTGGCCTTAACAGGGGATTTAAATGCCCTGTTACCAGAATTAcagc > 2:481359/1‑228 (MQ=255) tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGAGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGCCAGGCACATGTGGCATTACAAGGGAATTTCAATGCTCTGTTACCAGCATTAcagc > 1:125724/1‑228 (MQ=255) gCTGGGGATGCAAGGCA‑CCAAAGCGGCAATCTTGGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAcagca < 1:366197/228‑1 (MQ=255) cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACTTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCACACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAACGAACAAGTTGGGTCAGGCACATGTGGACTTACACGTGGCTTTTAATGCCCTGTTCACACCCTTAcacatg > 2:80564/1‑224 (MQ=255) cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACCCGCCAGTGTTATCCATATGGAAATCGACCCGGCAGAAATGACCAAGCGGCGTCAGGCACATGTGGCATTACAAGGGGATTTAACTGCCCTGTTCCCACCATTAcagcac > 2:328162/1‑227 (MQ=255) aTGCAGGGCA‑CCAAAGCGGAAAACTTCGGGGTGCAGGAGTGTAACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGtt < 1:523848/227‑1 (MQ=255) gcgcGGCC‑CAAAAGCGGCAAACTGCGGGGGGCAGGAGTGTGACCTGCTGATCGCCGTGGGCACAGGTTTGGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACGCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa < 2:266602/224‑1 (MQ=255) cACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt > 1:506084/1‑228 (MQ=255) gCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAAt > 1:100104/1‑228 (MQ=255) gCAAACTTCGCGGTGCGGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGACATGAACAAGCTGCGTCAGGCACATGTGGCCTTACAAGGTGATTTAAATGCTCTGTTACCACCCATACAACAGCCGTTCAATCAAGGAcagtcacca > 2:146484/1‑220 (MQ=255) gCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGCCCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCCATGACTGgcagca > 1:41996/1‑228 (MQ=255) aaCTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACAGGTAGCaaa > 1:447020/1‑226 (MQ=255) cAGGGGTGGGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCt < 1:566142/228‑1 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTTGGTCAGGACCCTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATTCATGGCAGCAACA‑CTCCGCGCGACGGCGtggg > 2:78691/1‑225 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGCACAAGCTGCGTCAGGCACATGTGGCATTACAAGTGGATTTAAATCCTCTGATACCACAATTACAGGAGCCGTTCACTCAATGACTGACAGAAACA‑CACCGCACAACTGCGcgagg > 2:142521/1‑226 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCCGAAAAA‑CTGCCCGCAGCTGCGtgat > 1:466501/1‑227 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACCGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCAAATGTGGCATTACAAGGTGATTTAAATACTCTGTTACCAGCATTACCGCAGCCGTTAAATCAATAACTGTCAGCACCA‑CTGAGcacacacgcgcgcgg > 2:443824/1‑217 (MQ=255) aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGTCAGCAAAA‑CTTCGCGCCGCTCCGtgatgac > 1:326751/1‑227 (MQ=255) ccTGGTGATCGCCGGGGGCGCCGGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa < 1:512286/227‑1 (MQ=255) ccTGCTGATCGCCGGGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa < 1:102748/227‑1 (MQ=255) cGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTGTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CGGCGCGAACCTGCGGGTTGAACAATCCTgg > 2:271976/1‑227 (MQ=255) cACTTTTTGGTGACCGGGTGCCCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt < 2:636375/228‑1 (MQ=255) tgatgaCCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGGGATGAACATTCCTGGCGTTACAACCAAccccgg > 2:102851/1‑224 (MQ=255) ccGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAGGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGG‑ACCAAAACCTGCCGGCACCCCCGTGATGAAAATTCCGTGCGTTACAGCCATCCCGGGGAcccca > 2:340804/1‑224 (MQ=255) taCCGGCAAACTGAACACCTTCGCGCACCACGCCAGTGTTATCCATATGGATAGCGACCCGGTAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg < 1:645925/227‑1 (MQ=255) ggCAAACTGAACACCTGCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCTTCAGGCAAATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgcg < 1:481359/226‑1 (MQ=255) aaaCTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt < 1:473057/228‑1 (MQ=255) ttaaCTCCTTCGCGCCACAACCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt < 1:271976/225‑1 (MQ=255) agtCGGGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa < 1:50731/225‑1 (MQ=255) cgcgCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaaa > 2:364298/1‑126 (MQ=255) gcgcCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt < 1:364298/126‑1 (MQ=255) cgcCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTCAATCAATGACTGGCAGCAACA‑CTCCGCGCAGCTGGGTGATGAAAATTCCTGGCGTTACGACCATCCCGGTGCCCACATCAACGCCCCGTTGTTTTTAAAAAAACtt > 2:494948/1‑227 (MQ=255) ccACACGCCAGTGTGATCCATACGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCTGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAACAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTGCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt < 1:80564/227‑1 (MQ=255) cacaCGCCTGTTTTATCCATATGGATATCGACCCGGCAAAAATGAACAAGCTGCGTCAGGCACATGGGGCATTACAAGGTGATTTAAATGCTCTGTTCCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg < 2:100104/228‑1 (MQ=255) cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGCTGATTTAAATGATCTGTTACCAGCATTACAGCAGCCGTACAATCAATGACTGGCAGCAACA‑CTGCGCGCAACGACGTGATGAACATTCCTGGCGTTACGCCCATCCCGGCGACGCTATCCACGACCCGTTGTTTGTCAaacaaccgcccgc > 2:406353/1‑221 (MQ=255) gCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGCCGTTACGACCATCCCGGTGCCGCCATCTACGCGCCGTTGTTGTTAAAACAACTTCCGGATCg > 1:527380/1‑228 (MQ=255) cgttgtTTTCCATTTGGATAGCGACCCGGGAAAAATGAACAAGCTGCTCCAGGCCCATGTGGCATTACAAGGTGATTTAAATGCCCTGTTACCAGCATTAACGCAGCCGTTAAATCCATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa < 2:125724/225‑1 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGCCGCAATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 1:182614/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 1:154881/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCACATGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 1:158113/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGAGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 1:41845/1‑228 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACAAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGt > 2:270885/1‑136 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCTGTGTTGTTAAAACAACTGTCGGATCGTAAACCCACGGATTg > 1:250578/1‑228 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGACCGTAAACCTCCGAATTg > 1:594771/1‑228 (MQ=255) tGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGtg < 1:270885/136‑1 (MQ=255) ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAACACTGTCGGCTCGTAAACCTGCGGATTGcg > 1:55692/1‑228 (MQ=255) gATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt > 1:558340/1‑228 (MQ=255) ctCGCCCCGGAAGAAATGACCAAGCTGCGTCAGGCACAGGGGGCATTACAAGGTGATTTAAATGCTCGGTTACCAGCATTACAGCGGCCGTTAAAGCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt < 2:655720/227‑1 (MQ=255) atCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTTGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt > 1:578386/1‑228 (MQ=255) tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTACATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCACCA‑CTGCGAGCAGCTGCGTGATGAACATTTCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGCTGCTGTTAAACCAACTGTCGGTTCGTAACCCTGCGGATTGCGCCGTg > 1:160690/1‑228 (MQ=255) tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg > 2:117969/1‑228 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc > 1:116887/1‑228 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt < 2:180098/224‑1 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt > 1:180098/1‑224 (MQ=255) ccGTCAGAAATGAACAAGCTGCGTTAGGCACATGTGGCATTTCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac < 1:78691/228‑1 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt > 1:430137/1‑201 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTGGTTGTTAAAACAACTGACGGATCGTAAACCTGCGGAATGCGTCCTGACcaca > 1:96692/1‑228 (MQ=255) ggCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa < 2:430137/201‑1 (MQ=255) gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc < 1:102851/228‑1 (MQ=255) gAACAAGCGTGGGCAGGCACATGTGGACTTACAAGGTGATTTAAAGGCTCCTTTACCAGCATTACGGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGGGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc < 2:506084/228‑1 (MQ=255) cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg > 1:584873/1‑182 (MQ=255) cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg < 2:584873/182‑1 (MQ=255) gggggggcAGGCACAGGGGGCTATACAAGGGGAATGAAAGGCTCTGGTAACAGCATTAAAGCGGCCGTTAAAACAATGACTGGCAGCAAAA‑CGGCGCGCAGCTGGGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc < 2:41996/220‑1 (MQ=255) ccaTTTGGCATTACAAGGGTATTTAAATGCTCTGTTCCCAGCATTACAGCCGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTg < 2:635384/227‑1 (MQ=255) atttgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCTTCGTGACCCCAGATGTGGGGCAGCACCAAATGTGGGCTgcg > 1:635384/4‑228 (MQ=255) aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCCGCACCAAAAGTGGGCTgc > 2:446563/1‑227 (MQ=255) ttgggattttaaaGGTGATTTAAATCGTCTTTTCCCAGAATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CCGCGCGCAGCCGCGGGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcgc < 2:527380/217‑1 (MQ=255) ttACAAGTGGTTTGAAATGTTCTGGTGCCAGTATTACAGGAGGCGTTAAATCAATGTCTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATTAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca < 2:55692/227‑1 (MQ=255) ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGAAGTGGGGCAGCACCAAATTTGGGCTGGGCAGCACACCCCCca > 2:321911/1‑228 (MQ=255) | TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA > NC_000913/3951269‑3951720 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |