Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 272,543 T→G 15.1% intergenic (+354/‑37) insN → / → eyeA pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/novel sRNA, function unknown, CP4‑6 propahge

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913272,5430TG15.1% 132.9 / 2.2 71intergenic (+354/‑37)insN/eyeApseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/novel sRNA, function unknown, CP4‑6 propahge
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base G (10/3);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 7.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGA  >  NC_000913/272320‑272768
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   
cAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGCAGGTGtt                                                                                                                                                                                                                                  >  1:378248/1‑227 (MQ=255)
     aCCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGCACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGAAACGTAAGCACTGCCGGTCGAAGGGGTTTTGACa                                                                                                                                                                                                                            >  1:241998/1‑228 (MQ=255)
                    acAGGGTGTTTTACCCCGGTTACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAACACCg                                                                                                                                                                                                           <  2:305947/227‑1 (MQ=255)
                            aTGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTTCCGGTCGAAGGTGTTTTGACATGTATGCGAACACCtggggcgca                                                                                                                                                                                                   >  1:305947/1‑223 (MQ=255)
                                      gtGACCAGGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGACCCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTAt                                                                                                                                                                                           <  1:400831/226‑1 (MQ=255)
                                       tGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGGATGCGAAACACCGGGGCAGCAGCGTCTATc                                                                                                                                                                                          >  1:35346/1‑227 (MQ=255)
                                         aCAACGGGGATTGAGGCGAGATCCCTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACg                                                                                                                                                                                       <  1:156356/228‑1 (MQ=255)
                                                           agaTCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCCGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACACCGGGGCAGCCGCGTCTATCACGCTTGTGTTGTTGACTTCt                                                                                                                                                                     >  1:42439/1‑228 (MQ=255)
                                                           agaTCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAGGTATGCGAAACACCGGGGCAGCAGCGTCTATACCGCTTGCGTTGCTGACTTCt                                                                                                                                                                     >  1:592309/1‑228 (MQ=255)
                                                                          gatgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGCCGGGGTTTTGACATGCATGCGAAACACCGGGTCACCAGCGTCTATCCCCCTTCCGTTCCTGACTTCTGCCACCTTTCGgcaa                                                                                                                                                      >  2:142322/1‑228 (MQ=255)
                                                                          gatgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTTGCGgcaa                                                                                                                                                      >  2:564698/1‑228 (MQ=255)
                                                                           ttgttCCTCAAGGTTAGGTCCGGAAAGGTTGAAGAACCTAAACCCGTTAATCCGCCTCTGGGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag                                                                                                                                                     <  2:394426/224‑1 (MQ=255)
                                                                           atgatCCTCATGTTTAAGTACTGAAAGGTTGAAAAACAAGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag                                                                                                                                                     <  1:457284/228‑1 (MQ=255)
                                                                             tttcccaaaGTTTAAGCACTGAAAGGTTGAAGACCATGAACCCGTTAATCCCCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca                                                                                                                                                   <  2:241998/221‑1 (MQ=255)
                                                                             gatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca                                                                                                                                                   <  1:348915/228‑1 (MQ=255)
                                                                                ccTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAgg                                                                                                                                                >  2:193882/1‑228 (MQ=255)
                                                                                ccTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGCTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGCACGACGAGGTTTTGGCATGTATGCGAAACACCGAGCCAGCAGCGCCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAgg                                                                                                                                                >  1:619026/1‑228 (MQ=255)
                                                                                           aGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCAACACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAACCACCGAGGCAGCAACGTCAGTCACGCTTGCGTTGCTGGACTCTGCACACTTCCGACAAGCACGGCAAAaggagg                                                                                                                                     >  1:616868/1‑223 (MQ=255)
                                                                                                tGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAaaaaaggggggacgg                                                                                                                                >  2:259817/1‑217 (MQ=255)
                                                                                                 gAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGCGTTTTGACATGTATGCGAAAAACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATTAAGAGGGCgcaggg                                                                                                                              >  1:340454/1‑223 (MQ=255)
                                                                                                 gAAAGGTTGAACCACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCGGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTGTTGACATGTATGCGAAACACCGGGTCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTCCCAACGTGCGGCAAGCAAGGAAAAAAAGtcggacagg                                                                                                                              >  2:74350/1‑220 (MQ=255)
                                                                                                   aaGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCCATAACGCTTGCGTTGCTGCCTTCTCCCAACTTGCGGCAAGCAAGGATAAAGAGGGCGACgggac                                                                                                                            >  1:270221/1‑226 (MQ=255)
                                                                                                       ttGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGGCTATCACCCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAAAAACGAGGGGACGGGCAGcc                                                                                                                         >  2:463478/1‑227 (MQ=255)
                                                                                                                aTGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACg                                                                                                                                                                                       >  1:401599/1‑157 (MQ=255)
                                                                                                                 tGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGc                                                                                                                                                                                      <  2:401599/157‑1 (MQ=255)
                                                                                                                 tGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGGTGCTGACTCCTGCCAACTTGCGGCAAGCAAGGATAAAAAGTGCGACGGGCAGCCCCCCCAGTATg                                                                                                              >  1:269645/1‑228 (MQ=255)
                                                                                                                   gaCCGGTTTATCCGGCTTGGTGGGTTGAAAAGGGCCCCCCGGCCTACGGGATCGGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGGTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGcc                                                                                                            <  2:229230/227‑1 (MQ=255)
                                                                                                                   caCCCGTTAACCCGCCTCTTTGGGTTGAAACCGTCCCCACGGCCTACGGGAGCTGACAGGCCGTGGAGGAGGAACGGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTTTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCGCAGTATGcc                                                                                                            <  2:592309/227‑1 (MQ=255)
                                                                                                                   aaCCTGTTAGACCGCCTGTGGGGGTTGAAAGCGAGTCCACGGCGTACGTGATCGGACAGGACTGGAAGGGGGAACTGGCAGTGAACCGTAAGCACTGCGGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGcc                                                                                                            <  2:48373/228‑1 (MQ=255)
                                                                                                                         ttAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGGATGCCtagttc                                                                                                      >  1:156522/1‑223 (MQ=255)
                                                                                                                         ttAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATCCCTGAGTc                                                                                                      >  1:572643/1‑228 (MQ=255)
                                                                                                                          tAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTTTGCCAACTTGCGGCAAGCAAGGATAAAAGGGGCGACGGGCACCCTCCCCCGCAAGCCTGAGAcc                                                                                                     >  2:240629/1‑228 (MQ=255)
                                                                                                                                gCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGa                                                                                                         >  2:13460/1‑218 (MQ=255)
                                                                                                                                 ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTTTCGGCAAGCAAGGGTAAAGAGGGCGCCGGGCAGCCTCCTCCGTATGCCCGAGGCcagggaga                                                                                              >  2:478303/1‑227 (MQ=255)
                                                                                                                                 ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAg                                                                                                        <  1:13460/218‑1 (MQ=255)
                                                                                                                                 ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGAAGCCTCCTCAGTATGCCTGGGTCcaggcagg                                                                                              >  1:48227/1‑228 (MQ=255)
                                                                                                                                 ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACCCTTGGGTTGCTGACTTCTGCCAACTTCCGGCAAGCAAGGGTGAAGAGGGCGACGGGCAGCCTCCCCCGTATGCCCGAGTcccagaagg                                                                                              >  2:332485/1‑221 (MQ=255)
                                                                                                                                    ctGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGAGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCCGACTTCTGCCAACTTGCGGCAAGCCAGGTTAAAGAGGGCACCAGGCGGCCTCCTCAGAATGCCCAGGGCCAGGCAGGGaa                                                                                           >  1:622274/1‑228 (MQ=255)
                                                                                                                                      gtgGGTTGAAAACGACCCCCGGGCCTACGTGATGTGAAAGGCCGTGCGGGAGGAAGTGGGTGTGATACGTAAGGAGTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAAc                                                                                         <  2:42439/228‑1 (MQ=255)
                                                                                                                                            tGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGa                                                                                   >  1:506308/1‑228 (MQ=255)
                                                                                                                                                   gTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAg                                                                            >  1:212523/1‑228 (MQ=255)
                                                                                                                                                     caccacGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGAATGCCTGAGTCCCGGAAGGGAAACCGGGGAAGGTCACCg                                                                          >  1:308157/1‑228 (MQ=255)
                                                                                                                                                        ccgggCCTACGTGATCTGACAGGCCGTACAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACGTGTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg                                                                       <  1:142322/225‑1 (MQ=255)
                                                                                                                                                        cccGGCCTGGGTGCTCTTAGCGGCGGTGGGGGAGGAACTGGGAGTGAGACGGAAGCCCTGCCGGTCGAAGGTGTTTTGACATGTATGAGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg                                                                       <  2:36773/226‑1 (MQ=255)
                                                                                                                                                           ggCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGGTATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAt                                                                    <  1:616746/228‑1 (MQ=255)
                                                                                                                                                             ccTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGt                                                                  >  1:505018/1‑228 (MQ=255)
                                                                                                                                                               tACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAGGtt                                                                 >  1:143910/1‑227 (MQ=255)
                                                                                                                                                                tcGTGATCGGAAAGGCGGTGCAGGAGTAACTGGCAGGGAGACGTAAGCCCTGCCGGTCGAAGGGTTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACGTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGATGGATGTTaa                                                               <  2:626102/227‑1 (MQ=255)
                                                                                                                                                                 cggtaTCTGACAGGCCGGGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAg                                                              <  1:463478/224‑1 (MQ=255)
                                                                                                                                                                      tCTGACAGCCCGTGTAGTTGGAATTGGAAGTGTCTCGGACTCACTGGCTGTCGATTGTGTTTTGGGATGTAGGAGAAACACCGGGGCAGCAGCGTCTAGCACGCTGGCGTTGCTGCGTTCTGCAAACTTGCGGCAAGCAAGGATAAAGAGTGCGCCGGGCAGCCTCCTCAGGATGCATGAGTCCAGGCAGGTAATCCGGGGAAGGTCAGCGACGGATGTTAACGGGGc                                                         <  2:267585/228‑1 (MQ=255)
                                                                                                                                                                       ttGACAGGGCGTGCAGTGGGAACTGGCAGTGATACGGAAGCATTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGc                                                         <  2:48227/226‑1 (MQ=255)
                                                                                                                                                                       cTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCa                                                        >  1:65252/1‑228 (MQ=255)
                                                                                                                                                                          aCAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGATACCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAAGTAAAGGGGGCagg                                                      >  1:477272/1‑225 (MQ=255)
                                                                                                                                                                           cAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATgg                                                     >  1:66098/1‑227 (MQ=255)
                                                                                                                                                                                  tGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAGGTTAAGGGGGCATGGCTCCGATg                                             >  1:527254/1‑228 (MQ=255)
                                                                                                                                                                                          gaACTGGCAGTGATACGTAAGCACTTCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAcg                                          <  1:342025/223‑1 (MQ=255)
                                                                                                                                                                                                   gggATACGTAAGCCCTGCCGGTTGAAGGGGTTTTGACATGTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCgg                             <  2:527254/225‑1 (MQ=255)
                                                                                                                                                                                                     gATTCGTAAGCCTTGCCGGTCGAAGGGGTTTTGACATGTATGCGAAACAGCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc                          <  2:143910/228‑1 (MQ=255)
                                                                                                                                                                                                      gtACGTCAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACAGCGGGGCAGCAGCGTCTAGCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc                          <  1:326706/226‑1 (MQ=255)
                                                                                                                                                                                                      aTACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc                          <  2:212523/227‑1 (MQ=255)
                                                                                                                                                                                                       tACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTCTGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCtt                        <  1:91028/228‑1 (MQ=255)
                                                                                                                                                                                                          gTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGGCAGCGACGGATGTTAAGGGGGAATGGCTCCGTTGACGCGCGGGCTGGCGGAGCTcccc                     >  1:569177/1‑227 (MQ=255)
                                                                                                                                                                                                              gctgtGCCGGGCGAAGGTTTTTTGACATGTATGCGAAACACCGGGGGCGGCGCTCCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgtag                  <  2:65252/223‑1 (MQ=255)
                                                                                                                                                                                                                  tGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGACGGTCAGCGACGGCTGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgtaggcgtc             >  1:305614/1‑223 (MQ=255)
                                                                                                                                                                                                                  tGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCACAGACGGATGTTAAGGGGGCATGGATCCCATGACGCGCTGGCGGCCGGAGCTTCCgtagtatcc             >  1:548129/1‑225 (MQ=255)
                                                                                                                                                                                                                    ccGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGAAGTcc            >  1:438478/1‑227 (MQ=255)
                                                                                                                                                                                                                        tCGAAGGTGTTTTGACATGTATGCGAAACCACGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGa        <  1:259817/227‑1 (MQ=255)
                                                                                                                                                                                                                             gttgtTTTGACGTGTATGCAGAACCCCGGGGCCGCAGCGGCTATCCCGCTTGCGTTGCTGCATTCTTCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGGCGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGATGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa  <  2:269645/226‑1 (MQ=255)
                                                                                                                                                                                                                             ggTTTTTTGACATGTATGGGAAACCCCGGGGCAGCAGGGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa  <  2:66098/228‑1 (MQ=255)
                                                                                                                                                                                                                             ggTGTTTTGACATGGTATGGAACCCCCGGGGGAGAGGCGGTTATCACGCTTGGGTTGCTGGCTTCTGCCAACTGGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa  <  2:569177/228‑1 (MQ=255)
                                                                                                                                                                                                                             ggTGTTTTGACATTTATGCGAAACACCGGGGCAGCAGCTTCTATCCCGGTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGGCGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa  <  2:506308/228‑1 (MQ=255)
                                                                                                                                                                                                                             ggTGTTTTGACATGGATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa  <  1:240629/228‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   
CAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGA  >  NC_000913/272320‑272768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: