Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,609,767 T→G 24.6% G118G (GGT→GGG prfC → peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,609,7670TG24.6% 73.9 / 3.0 55G118G (GGT→GGGprfCpeptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base T (16/21);  new base G (12/3);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 2.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTATCTC  >  NC_000913/4609551‑4609989
                                                                                                                                                                                                                         |                                                                                                                                                                                                                              
aaaaGGCCGTGGGTCCAACCAGTCCGGTAAGGCGGGCTGGATGGAGGTGGAAAAGCAGCGTGGGATCGCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCg                                                                                                                                                                                                                     <  2:642081/228‑1 (MQ=255)
         tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGTTACCTATCGTACCCTGACGGCGGTGGACTGCTCCCTGTTGGTTTTCGACGCCGCCAAGGGGGTGGAAGATCGTACCCCTaa                                                                                                                                                                                                            >  2:119087/1‑228 (MQ=255)
         tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTCCTGCCTGATGGTTATCGACTCCGCAAAAGGGGTTGAGGATCGTAccccaca                                                                                                                                                                                                            >  1:651544/1‑224 (MQ=255)
         tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTAACGGCGTTGGACGGCTGCCTGATGGTTACCGACCCCGCAAAAGGGGTTGAGAATCGTACCCGTaa                                                                                                                                                                                                            >  2:651452/1‑228 (MQ=255)
         tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCCGACGGCGGTGGCATTCTGACGTGTGGTGATGGACGCTGCAAAAGGCGTTGCAGAGCgacccccg                                                                                                                                                                                                              >  1:364884/1‑219 (MQ=255)
         tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGCACCCTGACGGCGGTGGACGGCTGCCTGATGGTTATCGACTCCGCACAAGGGTTTGCAGATCGTACCCGGaa                                                                                                                                                                                                            >  1:83436/1‑228 (MQ=255)
                      cACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGtt                                                                                                                                                                                               >  1:557843/1‑228 (MQ=255)
                                   aCTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGCGGACTGCTGCCTGCTGGTTATCGACGCCGCCAAAGGAGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTccgc                                                                                                                                                                                   >  1:343102/1‑227 (MQ=255)
                                         tggagatggaAAAGGGGGGGGGGTCCTCCATTTCTTCGCCTGGGATTCAGTTTCCGTTCACCGATTGCCGGGTTAACCGGCTCCACACCCCGGGGCACGAAGACTTCCCGGAAGTTACCTATCTTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACACGc                                                                                                                                                                            <  2:557340/228‑1 (MQ=255)
                                           gagatggaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCGGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGcc                                                                                                                                                                           <  1:32956/227‑1 (MQ=255)
                                                 gaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGCCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTTGGCGGGACACGCCGGTCCTc                                                                                                                                                                    >  1:241131/1‑228 (MQ=255)
                                                  aaaaGCAGCGTGGGATCCCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACCCCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAGGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCa                                                                                                                                                                   <  1:119087/228‑1 (MQ=255)
                                                  aaaaGCAGCGTGGAATCTCCATTACTTCGTCTGGTATGCAGTTTCCGTATCACGATTGCCTTGTTAACCTGCTAGACACCCCGGTGCACGAAGACTTCTCGGAAGATTCCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCa                                                                                                                                                                   <  1:358794/228‑1 (MQ=255)
                                                           gTGGGATCTCCATTACGACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATg                                                                                                                                                                                                     <  1:382743/185‑1 (MQ=255)
                                                              ggctctcCTTTACTTCGGTTGTGGTGCGTGTTCCGTATCCCGCTTCCCTGGTTACCCGGCTCGCCACCCCGGGGATCGAAGTTGTCTCGGACTATATCGATCGTACCTGTACTGCGTTTGACTGCTGCCTGATGGTGATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCTTAATCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAAc                                                                                                                                                        <  2:435966/224‑1 (MQ=255)
                                                                  ctcCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGCCTGATGCCCGATTGTTATCGACTCCGCAAAAGGTGTTGACGAGCGCACCCGTAAGCTGACGGAAGTTACCAGTCTGCGCGCAACGCCCACTCTGCCCCTTCAGAACAAACt                                                                                                                                                   >  2:239820/1‑228 (MQ=255)
                                                                        ttcttcGTCTGGTATGGAGTTCCCGTATCACGTTTGCCTGGTTACCCTGCTCGACCCCCCGGGGCACGAAGGCTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCg                                                                                                                                             <  2:607790/223‑1 (MQ=255)
                                                                             cGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGCGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGGGCGCCACCCCGCTCCCCCCCTTTTTGAAACAAATTTACCgggtaa                                                                                                                                        >  2:445492/1‑223 (MQ=255)
                                                                             cGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTCCCTGATGGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCACAACGCCAATCCCAACCATTATGAAAAAAATTGAcctgacaa                                                                                                                                        >  2:11400/1‑222 (MQ=255)
                                                                               tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACACGTCTGCTCGACACGCCGATCCTCACCCTTATGAACAAACTTGACCGTGAGATc                                                                                                                                      >  1:362558/1‑228 (MQ=255)
                                                                               tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGAAAGTTACCCGTCTGCCGGACCCGCCGTTCCTCACCTTTATGAACAAACTTGACCGTGATATc                                                                                                                                      >  1:468743/1‑228 (MQ=255)
                                                                               tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGGGTTGGAGATCGTACCCCTAAGCTGATGGAAGTTACCCGCCTGCGCGCCCCCCCGAGCCCCACCTTTTTGAACAAACTTCACCGGGAAATc                                                                                                                                      >  2:599114/1‑228 (MQ=255)
                                                                               tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCCGATGGTTATCGACGCCCCAAAAGGTGTTGACGATCGTACCCGGAAGCCGATGGAAGCTCCCCGCCTACGCGAAACCCCCCACCACACATTTATGACCAAACTTGACCGCGATATc                                                                                                                                      >  2:55621/1‑228 (MQ=255)
                                                                               tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTGCTGCCTGATTGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGTAAGCTGATGGACGTTACCCGTACGGCCGCCACGCCGACCCCCCCCTTTATGAACCAACTTAACCGGGATATc                                                                                                                                      >  2:394446/1‑228 (MQ=255)
                                                                                   tgATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGTTACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCACCGCCGCAAAAGTGGTTGAAGACCGTACCCGTAAGCTGATGGAAGTTACCCGTCCGCGCTAAACACCGATCCTCACCTTTATGAACAAACTTGACCGTGAGATcgcc                                                                                                                                   >  1:352612/1‑224 (MQ=255)
                                                                                   tgATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGACTGCGCGACACGCCGATCCTCACCCTTATTAACAAACTTTAACGTGATACCcgc                                                                                                                                   >  1:412222/1‑227 (MQ=255)
                                                                                             tCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTTACCGTGATATCGACGACCCGATggc                                                                                                                        >  1:340991/1‑227 (MQ=255)
                                                                                               gctATCACGATTGCCTGGTTAACCTGCCCGACCCCCCGGGGCACGAAGTCTTCTCGGAAGATACCTTTCGTTCCCTGACGGCGTTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGAAGGAg                                                                                                                       <  1:484370/225‑1 (MQ=255)
                                                                                                     aCGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAAATTGACCGTGATATCCGCGACCCCATGGAGCTGCTCg                                                                                                                >  1:49246/1‑228 (MQ=255)
                                                                                                        gATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCg                                                                                                                <  1:11400/226‑1 (MQ=255)
                                                                                                        ctttGCTTGGTTACCCTTCCCGCCCCCCCGGGTACCGAAGCCTTCTCGGAAGATACCGATCGTACCCTGACGGCGGGGGACTGCTGCCTGAAGGTTGTCGACGCCGCAAAAGGTGTTGAAGACCGTACCCGTAAGCGGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGAt                                                                                                              <  2:309160/226‑1 (MQ=255)
                                                                                                        aatggCCGGGTTAACCTCGTGAACACCCCGGGGCACGAAGACTTCCAGGAAGGTACCTACCGTGCCCTGACGCGGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTTTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGAGCCGATGGAGCTGCGCGAt                                                                                                              <  2:390490/224‑1 (MQ=255)
                                                                                                         aTTGCCTGGTTAACCTGCTCGACCCCCCGGGGCACGAAGTCTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGAt                                                                                                              <  1:423108/227‑1 (MQ=255)
                                                                                                                 gTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGGCCTTCTCGATGAAGTTggg                                                                                                     >  1:148189/1‑226 (MQ=255)
                                                                                                                  ttACCCTGTTCGACCCCTCGGGGCACGAAGACTTCTGGGAAGATCCCTATCGTGCCCGGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGGCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                                                                                    <  2:412222/228‑1 (MQ=255)
                                                                                                                     cccTGCCCGACACCCGGGGGCAGGAAGACTTCGCGGAAGATACCAGCCTTACCGTGAGGGCGGTGGCAGGCTGCCTGATGGTTAGCGAGGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGGCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCTACCCGATGGAGCTGCTCGATGAAGTTGAGAACg                                                                                                 <  2:455621/227‑1 (MQ=255)
                                                                                                                                                                   tCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAAATTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGCACGAGCTGAAAATCGGCTGTGGGCCGGTCCCCCGGCCGATTgggcgggg                                                   >  1:306128/1‑222 (MQ=255)
                                                                                                                                                                   tCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGAAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGAGGAAGTTGAGAACGAGCTGAAAATCGGCTGTGGCCCGATCACCTGGCCGATTGACTGCgg                                                   >  1:73296/1‑228 (MQ=255)
                                                                                                                                                                        cctgggcgggggTGGCCTGCTCCCGTAGGGTTATCGGCGCCGAAAAGGGTGTTGAAGACCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTAGGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                                               <  2:651544/221‑1 (MQ=255)
                                                                                                                                                                        cccTGCCGGCTGTGGACTGCGGCCTTATTGTTAGCGACGCAGAAAAAGGTGTTGAAGATCGTACCCGTAAGCTTATGGAAGTTACCCGTCTGGGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                                               <  1:223238/227‑1 (MQ=255)
                                                                                                                                                                        cccTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCCCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGACGTTGGAAGCGAGATGCAAATCGGCTGTGACCCGACCACCTGGCGGATTGGCGGGGGCacca                                              >  2:240443/1‑225 (MQ=255)
                                                                                                                                                                        cccTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAACTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGTTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGGGCGGAGAACCTGCCCGATGGGCGGAGGAAAGc                                              >  2:269992/1‑228 (MQ=255)
                                                                                                                                                                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGGAGGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGtt                                          >  1:357315/1‑227 (MQ=255)
                                                                                                                                                                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGACCTGaaaa                                                                                        >  1:29098/1‑181 (MQ=255)
                                                                                                                                                                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGGTGAGAACGAGCTGAAAAACCGCAGTGCGCCGGTCACCTGGCCGATTGGGCGCGGCAACCCGttt                                         >  2:579154/1‑228 (MQ=255)
                                                                                                                                                                                         tgctgcCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTac                             <  1:445492/228‑1 (MQ=255)
                                                                                                                                                                                          gttcgcgtagggTTATCGCCCCCGCAAAGGGGGGTGAAGATCGTACCCGGAAGCTGATGGAAGTTACCCGTCCGAGCGCCACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTac                             <  2:49246/217‑1 (MQ=255)
                                                                                                                                                                                           ctgcCTGATGGTTATCGACGCCGCAAAAGGGGTTGAAGATCGTACCCGGAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTCCTCGATGAAATTGGGAACGAGCTGAAAATCGCCTGTGCGCCGATTACCTGGCGGATTGGGTGCGGCAAAAGGTTTAAAGGGGTTTaca                            >  2:600999/1‑226 (MQ=255)
                                                                                                                                                                                             cctgtaTGTTTTTCGACGCGGCAAAAGGTGTTGAAGACCGTACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTaccac                          <  2:241131/222‑1 (MQ=255)
                                                                                                                                                                                                        aTCGATGCCGCAAAGGGGGTTGAAGATCGTACCCGTAAGGTGAGGGAAGTTCCCCGTCTGGGCGGCCCGGCGATCCTCCCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGAc              <  2:148189/228‑1 (MQ=255)
                                                                                                                                                                                                        aTCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGAGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCAGCTGTGCGCCGAGCACCTGGCTGATTGGCTGCGGCAAGCGTGTTAAAGCCCTTTa                              >  1:137793/1‑212 (MQ=255)
                                                                                                                                                                                                         tggACCCCTCAAAAGGTGTTGAAGATCTTCCCCGTAAGCGGATGAAAGTTACCCGTCTGCGCGACACGCCGATCCTCCCCTTTATGAACAAACCTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACg             <  1:225100/226‑1 (MQ=255)
                                                                                                                                                                                                              gcctcAAAAGGTGTTGAAGCTCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAAc         <  1:599114/227‑1 (MQ=255)
                                                                                                                                                                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCTGTTACACCCTTTTTAAAAACGAAAccat      >  2:442262/1‑226 (MQ=255)
                                                                                                                                                                                                                    aaaGGTTGTGAAGTTCTTCCCCGTAAGGTTATGGAGATTCCCCGTCGGGCCACCCCGCCGCTCCCCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGAGGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTAtctc  <  2:357315/228‑1 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                              
AAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTATCTC  >  NC_000913/4609551‑4609989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: