Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,825,226	G→T	15.6%	Q134K (CAG→AAG)	spy ←	periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,226	0	G	T	15.6%	208.2 / 3.8	82	Q134K (CAG→AAG)	spy	periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (27/41);  new base T (12/2);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 2.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGG  >  NC_000913/1825000‑1825464                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTAAGTTTTTTTGCTGAAAGATTATTCAGGAGTTGCAGGCCTTTTACCTTTTTCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAACTTATCATTTAATTGCTTTTTCTTTT‑CCGGGGTCAg                                                                                                                                                                                                                                       >  1:769892/1‑239 (MQ=255)  cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAAATTATTTAGCAGTTTCAGGCATTTTTCCTTTTTCCGCTTGG‑AGGTCTG‑TCAAACGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTT‑TCgggggcggg                                                                                                                                                                                                                                      >  2:778538/1‑232 (MQ=255)              aggTTAGGGGTAATCAGGTTTTTTTTACAATTGTT‑GGAAAAGAAAAAAGAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAAtt                                                                                                                                                                                                                         <  1:672657/237‑1 (MQ=255)                   aggggtgATCAGGTTTTTTTAACAGTTTTT‑GGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGtt                                                                                                                                                                                                                    <  1:286089/236‑1 (MQ=255)                    gtggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAATTTTTTTGCTGAAAAATTATTCTGCCCATGCATGCATTTTTCCTTTTGCCGCTGGACCTTTTTG‑TCACAC‑CTTCTCCAAATTAACATTTAATTGCTTTTTTTTTT‑CCGGCGCCCAGAGT‑TTGTAAAAttttttt                                                                                                                                                                                                                   >  2:677444/1‑238 (MQ=255)                     tggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTTTG‑TCTGCCGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTTTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGt                                                                                                                                                                                                                     >  1:557864/1‑236 (MQ=255)                          aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGCTTATTCCGCAGTTGCAGGCATTTTACCTTTTGCCGCTGTG‑CGTTCTG‑TCAGACGCCTCTCACAATAAGCATTAAATTTGTTTTTCTTTT‑CCGGGGTCAGGCTG‑TTGTTAATTTTGTTCTGGGtt                                                                                                                                                                                                             >  1:919049/1‑239 (MQ=255)                          aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAAGTTATTTAGCCGTTGCAGGCATTTTACCTTTTTCCCCTGGA‑CGGTCTG‑TCCGACGCTTCTCAAAATTATCATTTAATTGCTTTTTTTTTT‑CCGGCGTCCGGGTGTTTGGAATTTTTTTTTTGGGt                                                                                                                                                                                                              >  1:211242/1‑239 (MQ=255)                          aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTTGAAAATTATTCAGCAGTTTCAGGCCTTTTTCCCTTTTCCGCTGGG‑CGTTCTGTTCAG‑CGCTTTTCCAAATTAACATTTAATTGCTTTTTTTTTTCCCGGCGGCGGGTTG‑TGGTAAATTTTTTTTTggtt                                                                                                                                                                                                              >  2:858070/1‑237 (MQ=255)                            taGGTTTTTTTTCAAACTGTTGTGAAAAAAAAAAAAAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGttt                                                                                                                                                                                                            <  1:692184/238‑1 (MQ=255)                                      ttAAAAATGTT‑TGAAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                                                                                  <  1:568952/239‑1 (MQ=255)                                       aactgtggTT‑TGAAAAAAAAAAAAAACA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                                                                                  <  1:615513/230‑1 (MQ=255)                                         cAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTT‑TCAAACGCTTCTCAAAATTACAATTTAATTGCTTTTTTTGTT‑CCGGCGCCAGGATG‑TTGTAAAATTTGTTCTGGGTTTCCAttttggccacc                                                                                                                                                                                              >  1:909466/1‑229 (MQ=255)                                              tttttagaaagaaaaAAGAAAT‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                                                                                                                                                                                          <  1:352080/231‑1 (MQ=255)                                                        gagaaAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCCGTTGTCGGCATTTTACCTTTTGCCCCTGGA‑AGGTCTG‑GCCAACGCCTCTCCAAATTAGCCTTAAATTGATTTTTTTTTT‑TCCGCCGCCGGCTT‑GTGTAAATTTTGTTTTGGGGTTTCATTTGGGCCGACATTTTaagtgactc                                                                                                                                                                                >  2:615513/1‑231 (MQ=255)                                                         tgaaaagaaaa‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTGGCTGAAAGATTATTCAGCAGTTGCAAGCCTTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCCGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑CCGGCGTCAGGGTG‑GTGTAAATTTTGTTCTTGGTTTCCATTTGCGCCCGCCAGCTAGCttttc                                                                                                                                                                                 >  1:17515/2‑235 (MQ=255)                                                                  aacccgccgATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGttctt‑c                                                                                                                                                                      <  1:858070/236‑1 (MQ=255)                                                                   a‑CCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCa                                                                                                                                                                    <  1:794121/239‑1 (MQ=255)                                                                               tGTCCACCGCATTTCTGCAAGGTTGTGGACAAGGCCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATc                                                                                                                                                         <  1:778538/239‑1 (MQ=255)                                                                                gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTTTT‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTT‑TCGGAGTCAGGATG‑TTGTAAATTTTTTTTTTGGGTTTCATGTTCGCCAGCATGTTAGCTTTTGCCTGTTCTT‑CCCTTTTTTCGa                                                                                                                                                           >  1:128179/1‑236 (MQ=255)                                                                                     ccGCATTACTGGAAGGTAGGGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCt                                                                                                                                                  <  1:285902/239‑1 (MQ=255)                                                                                                   ggTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGGAGTTGCAGGCATTTTACCTTTTTCCGCTGGG‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCCTTAAATTTCTTTTTCTGTT‑TCCGGGGCAGGATG‑TTGTAAATTTTGTTCTGGGTTTTCATGTGCGCCAGCCTGTTAGCTTTTTGCTTTTTTT‑TCATTTTTGCGATC‑TGGGGTTTCCGTTTTACttt                                                                                                                                    >  2:92072/1‑239 (MQ=255)                                                                                                   ggTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTTCAAGCATTTTTCCTTTTTACGCTGGA‑CGTTCTG‑TCAGACGCTTCTCCAAATTAGCATTAAATTGCTTTTTTTGTT‑TCGGCGTCGGGATG‑TTGTAAATTTTTTTCTGGGGTTCCTTGTGCGCCATCATGTTAGCTTTTCGCTGTTCTTCCCATTTTTG‑GTTC‑TGCCGTTCAGCTTTTTCttt                                                                                                                                    >  1:133897/1‑239 (MQ=255)                                                                                                     tAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCg                                                                                                                                                            <  1:483229/214‑1 (MQ=255)                                                                                                      ggttgACAAAACCCGGGGTTTTAAGTGTTTTGGCTGAAAGATTATTCCGCAGTTGCAGGCATTTTTCCTTTTGCCTCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTGCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTGCTTTATc                                                                                                                                 <  2:31759/235‑1 (MQ=255)                                                                                                       gTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCCAAATTAGCATTTAATTGCTTTTTCTGTT‑CCGGCGTCCGGGTG‑TTTGAAAATTTGTTCTGGGGTTTCATTTTGCCCAGCATGTTTACTTTTCGCTGTTTTT‑CCATTTTTTGGGTC‑TTCGCTTTCGCTTTTTCTTTATCg                                                                                                                                >  1:914362/1‑239 (MQ=255)                                                                                                          gACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTTAGAAGTTGCAGGGATTTTTCCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGTTTTTTTTTTT‑CCGGCGGCCGGGTG‑TTGTAAATTTTTTTCTGGGTTTTCATGTGCGCCACCATGTTAGCTTTGCGCTTTTCTT‑CCCTTTTTTCTCTC‑TTCGCTTTCGCTTTTTCTTTTTCGAAg                                                                                                                             >  2:177908/1‑239 (MQ=255)                                                                                                          gACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAACTTTTGCCGCTGGA‑CGTTCTG‑TCAGAAGCTTCTCAAAATTAGCATTAAATTTCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGGAAATTTTTTTCTGGGTTTCCATTTGCGCCAGCCGTTTTGTTTTTGGCGGTTTTT‑CCATTTTTGCGATC‑TTGGCTTCGGCTTTTACTTTATctgag                                                                                                                             >  1:646786/1‑235 (MQ=255)                                                                                                          gACAAGACCGGCGGTCTTAAGATTTTTTGCTGAAAAATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCTCTTGA‑CGTTCTG‑TCAAACGCTTCTCCAAATTAACATTAAAATGCTTTTTCTTGT‑ACGGCGGCATGTTG‑TTGTAAAATTTGATCTTGGTTTCCATGTGGGCCAGCATGTTAACTTTGCGCATTTCTT‑CCATTTTTTCGATCATGC‑CTTCAACTTTTACTTTTTCGaaa                                                                                                                             >  1:647470/1‑238 (MQ=255)                                                                                                             aaGACCTGCGGTTTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGt                                                                                                                           <  2:637867/238‑1 (MQ=255)                                                                                                               gACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGCTG‑TTGTAAATTTTGTTCTGGGTTTCCATGTTCGCCAGCATGTTAGCTTTGTCCGTTTTTT‑CCATTTTTGGGGTC‑TGCGCTTTAGCGTTTTCTTTTTTGAAGGTAt                                                                                                                         >  1:889933/1‑238 (MQ=255)                                                                                                               gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCCAAATTAACATTTAATTGCTTTTTCTGTT‑TCCGCGTCCGGATG‑TTGTAAATTTTTTTTTGGGGTTTCCTTTGCGGCCGCCTGTTAGCGTTTCGCTGTTTTT‑TCCTTTTTTTGGTC‑TGCCCTTTACGTTTTACTTTTTCGGAgggaac                                                                                                                        >  2:225099/1‑235 (MQ=255)                                                                                                               gACCGGCGGTCGTAAGTTTTTTGGCTGAAAGATTATTCAGCAGATTCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTT‑TCAGACGGTTCTCAAAATTAACATTAAATTGCTTTTTCTGTT‑CCGCCGTCCGGGTG‑TTGTAAATTTTGTTTTGGGTTTTCATTGTCGCCCGCCAGTTGGCTTTTCGCCGTTCTT‑CCCTTTTTTCGCTT‑TGGGCTTCCGCTTTTTCTTTATTGCAGGTAtt                                                                                                                        >  2:836289/1‑238 (MQ=255)                                                                                                                  cggctgTTTTATTTTTTTTGGGTGAAAGATTATGCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                     <  2:552737/239‑1 (MQ=255)                                                                                                                        tttttaGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCCGGCATTTTTCCTTTTGCCGCTGGA‑CCGTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:66474/233‑1 (MQ=255)                                                                                                                        ttttAAGTTTTTTGGTTGAAAGGTTTTTCAGCAGTTGCAGGAATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:323026/236‑1 (MQ=255)                                                                                                                        ttctAAGTTTTTTGGGTTAAAGGTTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  1:891995/235‑1 (MQ=255)                                                                                                                        tCTTTAGTTTTTTGGGTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  2:586762/238‑1 (MQ=255)                                                                                                                        tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                <  1:187490/238‑1 (MQ=255)                                                                                                                         tttAATTTTTTGGGCTGAAAGTTTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                              <  2:185017/238‑1 (MQ=255)                                                                                                                           ttaGTTTTTTGGCTGTAAGTTTTTTCAGCATTTGCAGGCATTTTTCCTTTTGCCGGTTGA‑CGTTCTG‑TCAGACGCGTCGTAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgat                                                                                                            <  2:424309/237‑1 (MQ=255)                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACATTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTCAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCATTTTTGCGATC‑TTCGCTTCAGCTTTTACTTTATCGAAGTGAACGCTAGCAATGATGTCCTGc                                                                                                     >  2:485277/1‑239 (MQ=255)                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCAt                                                                                                       >  1:449612/1‑237 (MQ=255)                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGc                                                                                                     >  2:319014/1‑239 (MQ=255)                                                                                                                                  tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCCCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCTTCAGCTTTTACTTTATGGAAGGTATCGCTGGCAATGATGGCATga                                                                                                     >  2:878432/1‑238 (MQ=255)                                                                                                                                         gAAAGATTATTCAGTAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATGTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                                                                                              <  1:906285/239‑1 (MQ=255)                                                                                                                                         gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAAtt                                                                                                                                                                                                                         <  1:124531/118‑1 (MQ=255)                                                                                                                                         gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAAtt                                                                                                                                                                                                                         >  2:124531/1‑118 (MQ=255)                                                                                                                                                    cagcagTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTcc                                                                                   >  2:231589/1‑239 (MQ=255)                                                                                                                                                    cagcagTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCATGCATTGCGCGGCGtttttca                                                                                   >  2:129119/1‑238 (MQ=255)                                                                                                                                                           tgttggCTTTTTGCCTTTGGCCGTGGGA‑GGTTTTG‑TCAGGCGGTGCTCAAAAATAGCATTAAAGTGATTTTTCTTTT‑CCGGCGTCAGGATG‑TTGTAAATTTGGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTT‑CCAGTTTTGCGATC‑TGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATTCATTGCGCGGCGTTCTCTCAgctgag                                                                            <  2:17515/235‑5 (MQ=255)                                                                                                                                                           tGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                            <  1:92072/239‑1 (MQ=255)                                                                                                                                                            gCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGGGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGGTC‑TGCGCGTCAGCTTTTACTTTATCGAAGGATTCGCTGGCAATGATGGCATGCCTTACGGGGCGTTCTTCCAGcggcgg                                                                           >  2:520840/1‑239 (MQ=255)                                                                                                                                                                 cATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCACGCGGCGGCCGtt                                                                      >  1:714361/1‑239 (MQ=255)                                                                                                                                                                     ttACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAt                                                                  <  1:319014/239‑1 (MQ=255)                                                                                                                                                                             tGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGTCGGACGGTTCATCTGGTCAc                                                          >  1:134831/1‑239 (MQ=255)                                                                                                                                                                              gCCGGTGGA‑CCTTCTG‑TCAGACGCTTCCCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACg                                                         <  1:225099/239‑1 (MQ=255)                                                                                                                                                                                                   gccGCTTTTCAAAATTAGCTTTAAATTGTTTTTTTTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGGTCTGGTTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                      <  2:909466/237‑1 (MQ=255)                                                                                                                                                                                                     cGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGTCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcgcg                                    >  1:554642/1‑239 (MQ=255)                                                                                                                                                                                                      gCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACCCTGGCCTTTCATGATTTCGCgg                                   >  1:704885/1‑239 (MQ=255)                                                                                                                                                                                                         tttccaaaTTGCCATTAAATTTTTTTTTTTGTT‑CCGGCCGCTTGATG‑TTTTAAATTTTGTTCCTGGTTTCCATTGGCGGCAGCATGTTAGGTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTCCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATc                                <  2:429897/234‑1 (MQ=255)                                                                                                                                                                                                          ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCCTGCATTGGGCGGCGTTCTTCCAGTGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCTTGATTTCGGGGATcg                               >  2:320973/1‑238 (MQ=255)                                                                                                                                                                                                          ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGAttt                                        <  1:607222/230‑1 (MQ=255)                                                                                                                                                                                                          ctcAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                                  >  1:66460/1‑236 (MQ=255)                                                                                                                                                                                                          ctcAAAATTAGCATTAAATTGCTTTGTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGTTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCACCGGCGGCCTTTTCATCTGGTCACGCTGGCCTTTCTTGATTTCGGGGa                                  >  1:863187/1‑236 (MQ=255)                                                                                                                                                                                                                    gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgatg                                                                                                           >  1:733238/1‑153 (MQ=255)                                                                                                                                                                                                                    gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatgatg                                                                                                           <  2:733238/153‑1 (MQ=255)                                                                                                                                                                                                                    gCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGCCGGACGGTTCATCTGGGCACGCTGGCCTTTCATGATTTTGCGGGTctg                              >  2:607222/1‑230 (MQ=255)                                                                                                                                                                                                                       ttAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTgcg                   <  1:231589/238‑1 (MQ=255)                                                                                                                                                                                                                         aaaTTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGCCGGACGTTTCATCTGGTCCCGCTGGCCTTTCATGATTTCGCGGGTTTGCTGTTTTTGCGCGt                >  2:248651/1‑239 (MQ=255)                                                                                                                                                                                                                             ttgtttttCTGTT‑CCGGCGTCAGGTTG‑TTGTAAATTTTTTTTTGGGTTTCCATGGGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGt            <  2:128179/236‑1 (MQ=255)                                                                                                                                                                                                                             ttcTTTTTCTGTT‑GCGGCGTCAGGTTG‑TTGTAAATTTTTTTCTGGGTTTCCATGGTCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGt            <  2:714361/237‑1 (MQ=255)                                                                                                                                                                                                                              ttttttttTGTT‑CCGGCTTGAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  2:914362/237‑1 (MQ=255)                                                                                                                                                                                                                              gttttttCTGTT‑CCGGGGTTAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:889575/237‑1 (MQ=255)                                                                                                                                                                                                                              gCTTTTTCTGTT‑CCGGCGTTCGGAGG‑TTGTAAATTTTGTTGTGGGTTTCCATGTGCGCCAGCATTTTAGCTTTGCTCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:890255/239‑1 (MQ=255)                                                                                                                                                                                                                              gCTTTTTCTGTT‑CCGGCGTCAGGATT‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:433052/239‑1 (MQ=255)                                                                                                                                                                                                                              gCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:839606/239‑1 (MQ=255)                                                                                                                                                                                                                              gCTTTTTCTGTT‑CCGGCGGCAGGATG‑TTGTAAATTTTGTTCGGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTG‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:419575/239‑1 (MQ=255)                                                                                                                                                                                                                              gCTTTTTCTGGT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc           <  1:423688/239‑1 (MQ=255)                                                                                                                                                                                                                                   ttCTTTT‑CCGGTGTCAGGATG‑TTGTAAATTTTTTTCCGGGTTTCCCTTTTCGGCTGCATGTTTGCTTGTCGCTTTTCTT‑CCATTTTTGCTATC‑TTCGCGTCAGCTTTTACTTTATCGAAGGTAGCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTGCATCTGGTCACGCTGGCCTTTCATGATTTCGCTGATCTGCTGTTTCTTCGCGTCGGtcaggtt      <  2:66460/239‑1 (MQ=255)                                                                                                                                                                                                                                    tCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCGGTTTCTGCGCCTCGGtcgggttc     >  1:88145/1‑239 (MQ=255)                                                                                                                                                                                                                                     ttGTT‑GCGGCGGCTGGTTT‑TTTTAAATTTTTTTCTTGTTTTCCCTTTGCGTCCGGTTGGTAACTTTTCGCTGTTTTT‑CCATTTTTGCGATC‑TGCGCTTCGGCTTTTAGTTTATCGAATGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttca    <  2:878248/238‑1 (MQ=255)                                                                                                                                                                                                                                       gTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCAAGCGCCGGACGTTTCATCTGGGTACGCTGGCCTTTCATGATTTTGCGGGTTTGCTGTTTTTGGGGGGCGGggagggtcagg  >  2:419575/1‑239 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGA‑CGTTCTG‑TCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTT‑CCGGCGTCAGGATG‑TTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT‑CCATTTTTGCGATC‑TGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGG  >  NC_000913/1825000‑1825464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑