Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,877,287 A→C 21.5% G82G (GGT→GGG yoaF ← DUF333 family outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,877,2870AC21.5% 89.1 / 2.1 58G82G (GGT→GGGyoaFDUF333 family outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base C (5/10);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGG  >  NC_000913/1877049‑1877508
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
aaCGGAACTGCGCAAAGCCTTTCCCGGCGAAGGCGGCGATTATGCAACCTTGCGCGAGCAATATCTTGCAGAACTGGCGCAACCCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCa                                                                                                                                                                                                                                   <  1:849149/239‑1 (MQ=255)
 ccGGAACTGCGAAAACGCTTTCCCGGCGAAGTCGCCCATTAGGAAACCTTTCGCGAGCAATATCTTGCAGAACGGGCGCAACAGGAGAAAGAAGGAAAGCGGCGGGCGGACACCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCGGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAc                                                                                                                                                                                                                                  <  2:254527/238‑1 (MQ=255)
          cgcAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTTCCCAGCCGGAt                                                                                                                                                                                                                         >  1:650962/1‑239 (MQ=255)
             aaaCCCTTTCCCGGGGAATGCGTCGATGTTGCAACCTTTCGCGGGCAATATCTTGCAGAACGGGCGAAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGt                                                                                                                                                                                                                      <  2:785968/239‑1 (MQ=255)
               ctcttttCGCGGCGAATTCGTCAATTATGCACCCTTTGCCGAAAAATTTCTTGCGGAACGGGCCCAACGCGGGAAGGAAGGAAAGGGGCTGGCGGACATCGCCAAAAAACAGCCCAGGCCCCTGCACCACTCAGCAAAAAACACCACGCAGACCCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCAGCCGGATGGTCa                                                                                                                                                                                                                    <  2:219033/235‑1 (MQ=255)
               aGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTCCGTAGCTCGTGATTTTTGAACAGCATCCGGCGGTTTATTTTTGACCGCCGGATGGTc                                                                                                                                                                                                                     >  1:216042/1‑238 (MQ=255)
                                 gggttgttATGGAACTTTTCGCGCGAAAATTCTGGCCAAACGGGGCAAACCCGGGCAAGAAGGAAAGCGGCTGGCGAAAATCGCCAAAAACCAGCCGTGGACCCGGCCTTACTCAGCAAAAAACACCCCGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCa                                                                                                                                                                                                   <  2:521330/232‑1 (MQ=255)
                                       ttATGCAACTTGGCGCGAGCAATACCTTGCAGAACTGGCGCAACACGGACAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATtcatc                                                                                                                                                                                             <  1:539147/238‑1 (MQ=255)
                                                 tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAACCGGATGGTCACGCCGCCACACTGCCCCTTCATCAAACGtttaa                                                                                                                                                                                   >  1:125666/1‑236 (MQ=255)
                                                  ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGGGCTGGCCGACTGGCTACGTAACTTGTGATTTTAGTACAGCATCCGGCGGTTTTTTTTTCACACCCGGATGGTGAAGCCCCCCAAATGCCCCTTCATCAAATGtttacc                                                                                                                                                                                  >  2:310186/1‑235 (MQ=255)
                                                         gCAATATCTTGCAGAACTGGCGAAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACCCCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCgg                                                                                                                                                                           <  1:319524/238‑1 (MQ=255)
                                                             tatCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAGGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGcc                                                                                                                                                                              <  1:171076/231‑1 (MQ=255)
                                                                     aGAACGGGCGCAACCCGGGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCAGGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAAtt                                                                                                                                                               <  1:911398/238‑1 (MQ=255)
                                                                        aCTGGCGCACACCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAACCAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAAttgttg                                                                                                                                                           <  1:310186/239‑1 (MQ=255)
                                                                         cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGGTATTTTTTACCACCCGGCTGGGCACCCCGCCCCAACGCCCCTTCCTCATTCTCTTTTCCCCCCGCGACTTTTCCATTGTTGc                                                                                                                                                          >  2:591156/1‑239 (MQ=255)
                                                                           tgcgcAACCCGCGCAAGAAGGAAAGCGGCTGGCGGAAATCGCCAAAAAACAGCCGCTGACCCTGCTCTCCTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTg                                                                                                                                                        <  2:283121/238‑1 (MQ=255)
                                                                           ggCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGAACCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCCTTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTg                                                                                                                                                        >  1:133189/1‑239 (MQ=255)
                                                                              gcAACACGAGCAAGAAGGAAAGCGGCTAGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTCCAGCATCCGGCGGTTATTTTTCACCCGCCGGATGGTCACGCCGCCCCAATGCCCATTCATCAAACGGTTCCCCGCCCGGTAATTTGCAAATTTTGCTTAc                                                                                                                                                     >  1:382435/1‑238 (MQ=255)
                                                                              ccAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCAAAAAAACAGCCGCTGCCCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGAc                                                                                                                                                     <  1:535697/237‑1 (MQ=255)
                                                                                     gtcaaagaagGAAAGCGGCGGTGGGACCTCCGCAAAAACCACCCGGGGACCCTGTACTACCCAGCAAAAAACACCCCGCAGAACCATGCGCGGGTGCTGGCCGACTGGCAACGTAGCTTGGGATTTGAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTgcg                                                                                                                                             <  2:560969/235‑1 (MQ=255)
                                                                                      aGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCTCCCGCTAATTTGCCATTTTGTTCAccccctttc                                                                                                                                             >  1:914657/1‑232 (MQ=255)
                                                                                      aGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTAACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGGAATTTGCAATTGTTGCTTACCCCTTgc                                                                                                                                              >  1:123403/1‑237 (MQ=255)
                                                                                                tcaGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCCCCACAATGCCCCTTCCTCAATCGGTTTACCCCCCGGGAATTTGCAATTGTTTCTGACCCCCTTGCGTGTCTGc                                                                                                                                    >  1:857622/3‑236 (MQ=255)
                                                                                                aaaggcgCTGGCGGCAATCGCAAAAAACCAGCCGCTGACCCTGTTCTACTAAACAAAAAACACCCCGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCCTCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCa                                                                                                                                   <  1:82583/231‑1 (MQ=255)
                                                                                                 aaGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGGTATTTTTTTCCCACCGGCTGGGCACGCCGCAACAAAGCCCCTTCATCAATCGTTTCTCCCCCCCGGAATTTTCAATTGTTGCTCACCCCTCGCCCTGTCtctcctg                                                                                                                                 >  2:528836/1‑233 (MQ=255)
                                                                                                   gCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGCCTGGCTACGTAGCTTGGGATTTTAGTACAGCATCCGGGGGTTTTTTTTCCACAACCGGATGGTTACGCCGCCACAATGCCCATTCCTCAAACGTTTCACCGCCCGGCAATTTTCCATTGTTGCTTACCCCTTCCCCTGCCtcccccgga                                                                                                                               >  2:528861/1‑231 (MQ=255)
                                                                                                       cTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTTACCCCTTGCGCTGTCTGCACTGGAATg                                                                                                                           >  1:728330/1‑238 (MQ=255)
                                                                                                         ggCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCCCAATTCCCATTCATCAATCGTTTCACCCCCCGGGAATTTGCAAtt                                                                                                                                                               >  1:700604/1‑202 (MQ=255)
                                                                                                             gcaacgccaaaaaaaCCGCCGGGGACCCTGCTTTCGTCAGAAAAAACCACCACGCAGAACCATGGGCTGGTGCTGGCCGACTGGCTAGGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGAGGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTc                                                                                                                     <  2:799427/229‑1 (MQ=255)
                                                                                                             cacacccccAAAAAACCCCCGCTGCCCCTGCTCTCCCCCGCAAAAAACCCCCCGAAAAACCATGCGCTGGTGGTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCACCCGGCGGTTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTcc                                                                                                                    <  2:268738/232‑1 (MQ=255)
                                                                                                                        aaacCAGCCGCTGCCCCTGCTCTACTAAGCAAAAAACCCCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGGTATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTctgc                                                                                                         <  1:591156/239‑1 (MQ=255)
                                                                                                                                        ctcttctACCCACAAAAAACCACCCCGCAGAACCAGGGCGTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACg                                                                                         <  2:650962/235‑1 (MQ=255)
                                                                                                                                             ccaCTGAGGAAAAACCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGGACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCgta                                                                                    <  1:528836/237‑3 (MQ=255)
                                                                                                                                                cTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCCGCCGGATGGTCACCCCCCCCCAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTTCAATTGTTGCCGCCCCCTTTCCCTGTCTGCCCTGGAATGGGCGTCCCCCCCCTTTCCTGGCAAAAAACACCGGCCGGAttt                                                                                 >  2:5054/1‑239 (MQ=255)
                                                                                                                                                   aGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTTACCCCTTGCGCTGCATGCCACTGACTGAACGTCCCGCCCTCCGTCTGGCCATAAGCCGCGCCCGGGTTTGc                                                                               >  2:243509/1‑238 (MQ=255)
                                                                                                                                                     cAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCCATTTTTGCTGACCCCTTTCGCTGTCTTCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCAGGGATTGCCAt                                                                            >  2:202619/1‑239 (MQ=255)
                                                                                                                                                     cAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTTTTGCTGACCCCTTGCGCTGTCTGCACTGGGATTAGCGTCCCGCCCTTTTGCCGGCAAATAACCCACCCCGGCTTTGCCaa                                                                            >  2:324737/1‑238 (MQ=255)
                                                                                                                                                      aaaaacCCCCCCCCAAAACCATGCCCTGGTTCGGGCCGACTGGCTACGGAGCTTGTGATTTTAGTACAGCACCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATa                                                                           <  2:857622/239‑1 (MQ=255)
                                                                                                                                                        aaaaCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATAcc                                                                         >  1:575010/1‑239 (MQ=255)
                                                                                                                                                          aaCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTTCCATTCCaa                                                                       >  1:214289/1‑239 (MQ=255)
                                                                                                                                                               cacGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATTAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCttc                                                                  >  1:28631/1‑237 (MQ=255)
                                                                                                                                                                 cgcgGAACCAGGCGCGGGTGCTGGCCGACTGGCTACGTAGGTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:727722/239‑1 (MQ=255)
                                                                                                                                                                 cGCAGAACCAGGCGCGGGGGCTGGCCGACGGGCGACGTAGCTTGTGATTTTAGTACAGCAACCGGCGGTTATTTTTCACCAGCCGGATGGGCACGCCGACACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTGGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:614830/239‑1 (MQ=255)
                                                                                                                                                                  gCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:649710/238‑1 (MQ=255)
                                                                                                                                                                      acccATGCGCGGGTGCTGGCCCACGGGCTACGTAGCTTGTGATTTTAGTCCAGCACCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGt                                                           <  1:210317/236‑1 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCCATTGTTGCTGACCCCTTTCTCTGTCTGCCCTGGAATGAGCGTCCCGCCCTTCTGCTGGCCCACGACCGACGCCGGGTTTCCCCTACCAAACTTcgggggggttagg                                                     >  2:649710/1‑232 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGAc                                                                                              >  2:47036/1‑199 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGAc                                                                                              <  1:47036/199‑1 (MQ=255)
                                                                                                                                                                             cgcTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTCCGCCGGTTTTggggg                                                   >  1:350484/1‑236 (MQ=255)
                                                                                                                                                                                tGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTc                                                <  1:202619/239‑1 (MQ=255)
                                                                                                                                                                                tGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCCCCCGGTAATTTTCAATTTTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCCCCCTTCTGCTTGCAATCAACCGCAGCCGGATTTGCCCTACCAACCTCCGCCGGGTTTAgtgtacc                                                 >  2:560853/1‑233 (MQ=255)
                                                                                                                                                                                          ccaCTGGCTCCGTGTGTTGTATTTTTATTCCCGCCCCCGGGGTTTTTTTTCCCCCACCCGGATGTGCCCGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTgaga                                      <  2:568139/236‑1 (MQ=255)
                                                                                                                                                                                                                   ttgtccAGCCTCCGGGTGTTCTTTTTCCCCAGCCGGAGGGTCACGCCGCCACAATGCCCCTTCACCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCa            <  2:728330/234‑1 (MQ=255)
                                                                                                                                                                                                                   tggTACCGCCTCGGGGGTTTTTTTTTCCCCAGCCGGATGGTCACCCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCa            <  2:186625/237‑1 (MQ=255)
                                                                                                                                                                                                                   atgtcccgagaccGGTGGTTATTTTCCCCGACCGGGATGGCCACGCCGCCACCATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGCCCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCa            <  1:362162/228‑1 (MQ=255)
                                                                                                                                                                                                                        cAGCATCCGGCGGTTATTTTCCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCagcaag       <  1:428626/239‑1 (MQ=255)
                                                                                                                                                                                                                           cATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTATGGGGTTTCGGCTGCGAAAGGGGGTGAAAAACCCCCCGGACCCgcaaacaa    >  1:186625/1‑239 (MQ=255)
                                                                                                                                                                                                                             cccGGTGTTTTTTTTTCCCCAGTCGGTGTGTCCCGCCGCCACAATGCCCTTTCATCAATCGTTTCCCCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAgg  <  2:123403/238‑1 (MQ=255)
                                                                                                                                                                                                                             cccGGCGTTTTTTTTCCCCCCGCCGGATGGTCACCCCGCCACAATGCCCATTCCTCCATCGTTTCCCCGCCCGTTAATTTGCAATTGTTGCTTACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAgg  <  2:912761/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
AACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGACCCCTTGCGCTGTCTGCACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGG  >  NC_000913/1877049‑1877508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: