Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,355,458 T→G 14.5% G398G (GGT→GGG glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,355,4580TG14.5% 119.6 / 2.5 59G398G (GGT→GGGglpBanaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base G (10/2);  total (36/23)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGGCGCTGAAATATTGCATCA  >  NC_000913/2355231‑2355692
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
cGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGt                                                                                                                                                                                                                                  <  1:184332/239‑1 (MQ=255)
  tcaCGGCATTCGAGGGCCGGTTCCGGGCCTTTATTGGCTAAAAACCGCCACGGGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCa                                                                                                                                                                                                                                <  2:571204/237‑1 (MQ=255)
        cATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGGGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGCCGGGGTTTGTGCCGTCAGGGCtt                                                                                                                                                                                                                          >  1:755024/1‑239 (MQ=255)
          ttCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATTCGGCGGCGGGGTTTGTGGCGTCCGGGCTTTa                                                                                                                                                                                                                        >  1:392622/1‑239 (MQ=255)
            cGAGAGCCGATTCTCGGCCTTGATTGGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACa                                                                                                                                                                                                                      <  1:654278/239‑1 (MQ=255)
            cGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTTGGCCGTCAGGGCTTTaaa                                                                                                                                                                                                                      >  1:288255/1‑237 (MQ=255)
               ggggcggttCTGGGCCTGGATGGGCTAAAAACCGCCACGGGGGGGGAATGGGATAAGGGAGATTTTTTTGCGCCGCAACCGGGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATg                                                                                                                                                                                                                   <  2:45564/231‑1 (MQ=255)
                  ccGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTGTGCCGTCAGGCCTTTACAttcgc                                                                                                                                                                                                                 >  1:777201/1‑234 (MQ=255)
                                       cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCTCCCAGGGATGCGGCGCCGGGGTTGTTGCCGGCCGGGCTTTACATGCCGCTCACCAGATTTCCCaaaccc                                                                                                                                                                                           >  1:127465/1‑234 (MQ=255)
                                       cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTTGGCCGTCAGGGCTTTACATGCCGCCCaa                                                                                                                                                                                                           >  1:21637/1‑222 (MQ=255)
                                                 ccACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaa                                                                                                                                                                                >  1:89858/1‑239 (MQ=255)
                                                   aCGGGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGGGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaaca                                                                                                                                                                              <  1:791658/239‑1 (MQ=255)
                                                    cgcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTTGGCCGTCGGGGCTTTACATTCCGCTCCACAACTTGCCCAACCCGCACAGGGCcaaaaa                                                                                                                                                                             >  2:389386/1‑239 (MQ=255)
                                                       ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGAAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatg                                                                                                                                                                           <  1:320857/238‑1 (MQ=255)
                                                            gAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCCTCGGTTAGGTGCTGGGCGGATTTGATCCCCTCCCCCAGGCATGCGGCGCCGGTGTTTGTGCCGTCAGTCCTTTACATGCCGCCCCACCAATTGCCCAACCCCCAGGGGGCCCACCaagagtgaa                                                                                                                                                                     >  2:671706/1‑238 (MQ=255)
                                                                   ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCCACAATGAATGAAACCAGCt                                                                                                                                                              >  1:33123/1‑239 (MQ=255)
                                                                      gggggGATTTTTTTGCGCCGCAACCGTGGCAGGAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTACCAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGC‑‑‑GTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                                                                                                                                                        <  2:228047/238‑1 (MQ=255)
                                                                           taTTTTTTTGCCCCGCCACCGGGGCAGCCGTTCGGTTGAACCCCTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAACCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaaa                                                                                                                                                       <  2:873805/237‑1 (MQ=255)
                                                                           gaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCCACAATGAATGAAACCAGGTTCGaaaaa                                                                                                                                                      >  1:351102/1‑238 (MQ=255)
                                                                            ttttttttGCGCCGCACCCGTGGCAGAAGTTCGGTGTAACCACTGATGGGCCTCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGGCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                                                                                                                                                        <  2:636485/235‑1 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGCTGCGGCGGCGGGGTTTGTGCCGGCAGTGCTTTACATACCCCCCCAACAGTTGCCCAACGCCCAGGAGGCCCACAACAAATAAACCAAACTTCGaaaaac                                                                                                                                                     >  2:5995/1‑237 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCCGTGCCTTACATGCCGCCCAACAGATTTCCCAACGCGCAGGAGGCCAAAAAAGAATAACACCAACTTCGaaaaac                                                                                                                                                     >  2:257964/1‑237 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGGGGCCACCAATGCATGACAACAGCTTCGAAAACt                                                                                                                                                     >  1:841969/1‑239 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGGGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACCCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACt                                                                                                                                                     >  1:423276/1‑239 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACCCGCAGGAGGCCAACCATGAATGCCACCAGCTTCGAAAAc                                                                                                                                                      >  1:745334/1‑238 (MQ=255)
                                                                            aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGCGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTTGTCCGGCAGTGCTGTACATGCACCCCCACAGAGTTCCCACACCCCAGGGGGCCAACAAAGACTAACAACAGcaccatacagc                                                                                                                                                     >  2:173414/1‑229 (MQ=255)
                                                                              ttttttGCGCCGCAATCGTGGCAGCAGTTCGGTGTAACCTATGATGAGACGCTACGTCCGTCACAGGCAGGGCAAACCATTGAAAGCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGACCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGc                                                                                                                                                   <  1:425594/239‑1 (MQ=255)
                                                                                 tttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGGGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGGAAACTGCAtt                                                                                                                                                >  1:384308/1‑239 (MQ=255)
                                                                                      gccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCCGCTTCGAAAACTGCATTaa                                                                                                                                              >  1:636485/1‑236 (MQ=255)
                                                                                        cggaaCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCa                                                                                                                                         <  1:702734/236‑1 (MQ=255)
                                                                                                           cGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACCGGCAGGAGGCCAACAATGAATGGACCCAGCTTCGAAAACTGCATTAAGGGCCCCGCCTGCAccccccccg                                                                                                                       >  1:331210/1‑237 (MQ=255)
                                                                                                                  aCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGCGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGCAAACTGCATTAAGTGCACCGTCCGCACCACCCCCTTCCCCGt                                                                                                                >  1:705703/1‑238 (MQ=255)
                                                                                                                   ccACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACCGATTGCCCAACGCGCAGGAGGCCAACAATGAATGAACCCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCCCCTGCCCGGTgg                                                                                                              >  1:388689/1‑238 (MQ=255)
                                                                                                                                 cTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCCGGAGGCCAACAATGAATGCCACCAGCTTCGAAAACTGCATTAAGTGCACCCCCTGCCCCACCCCCCGCCCGGGGAGCCGGGGGAAAccc                                                                                                >  2:70855/1‑239 (MQ=255)
                                                                                                                                         gtccggggcaggGCAAACTATTAAAAACCTGTTTGCCAGCGGTTGGGGGCTGGGGGGATGTGACCCCATCGCCCGGGGATGCGGGGGGGGTGTTTGGGCCGACAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATcc                                                                                        <  2:89858/233‑1 (MQ=255)
                                                                                                                                                    ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCCGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGACCCACCGCCCGCCCGGGGAGCCGGGTGAATCCCGGTTATCCAGGGCCaaacc                                                                             >  1:5714/1‑237 (MQ=255)
                                                                                                                                                                     ctttttGCCATCGTTTCGGGTCTGGGGGGATTTGACCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGc                                                            <  1:5995/236‑1 (MQ=255)
                                                                                                                                                                        tttGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGGGAATCCCGGTTATCCAGGGCCAAACCAACCCGGGCCGGATGGCGAg                                                         >  1:356083/1‑239 (MQ=255)
                                                                                                                                                                                    tcggtGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTcgtcggttg                                             >  1:594535/1‑231 (MQ=255)
                                                                                                                                                                                         ggtGGGCGTTTTTGACCCCTGCGCCCGGGGTTGGGGGGGGGGTGTTGGGCCAGGCGGTGCTTCACAGGCCGCTCAAGAGATTCCCAAACGCGCGGGAGGCCACCAATGAATGCCACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCCCCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGa                                        <  2:33123/237‑1 (MQ=255)
                                                                                                                                                                                           tGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGCAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGGGAATCCCGGTTATCCAGGCCCAAAACAACCCGGGCGGGAGGGCGGGCGTCCGGGTTTGAAAGATg                                      >  2:699923/1‑239 (MQ=255)
                                                                                                                                                                                             ggCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCCCCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGAGGGCGGGCGTCTGCGTTTGAAAGAggg                                     >  1:759599/1‑238 (MQ=255)
                                                                                                                                                                                               gggttttGTTCCCCCCGCCCGGGGGTGGGGGGGGGGGTTTGGGGCGGTCAGTGCTTTACAGCCCCCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAAGGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGgcg                                   <  2:841969/234‑1 (MQ=255)
                                                                                                                                                                                               gggtttggaccccATCCGCCAGGGGGGGGGGGGGGGTGGTTGGGCGGTAAGGGCTTACCACGCCGCAAAAAGGATGGCCCAAGGGGAAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCGGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGgcg                                   <  2:705703/228‑1 (MQ=255)
                                                                                                                                                                                               gggATTTGCCCCCCTCGCCCAGGGATGGGGGGGGGGTTTTTGGGCCGTCAGTGCTTTACAGGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACCCCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGgcg                                   <  1:97584/237‑1 (MQ=255)
                                                                                                                                                                                               cGGATTTGACCCCTTCGCCCGGGGTTGCGGCGGCGGTGTTTGTGCCGTCAGTTCTTTACATGCCGGTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGgcg                                   <  1:257964/238‑1 (MQ=255)
                                                                                                                                                                                                 tttttGATCCCTCCCCCCAGGGTTGGGCGGGGGGTTTTTGGGTGTTCGGTGTTTTAAGTGCCGCTCAACAGAGTGCCCAACGCGCAGGCGGCCAACAATGAATGACCCCAGCTTCGAAACCTGCATTAAGTGCCCCGTCTGCACCCCCGCCTGCCCGGGGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCa                                 <  2:392622/236‑1 (MQ=255)
                                                                                                                                                                                                 gATTTGACCCCAGCGCCCGGGGATGGGGCGGCGGTTTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACCCCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCa                                 <  1:70855/238‑1 (MQ=255)
                                                                                                                                                                                                  aTTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATTACACCAGCTTCGAAAACTGCCTTCAGGGCACCGTCCGCCCCACCGCCCGCCCGGGGAGCCGGGTGAATCCCGGTTATCCCGGGCCCAAAAAAACCGGGCCGGGGGGGGAGCGGCCGCGTTTGGAAAAGGGCGCAcc                               >  2:641279/1‑238 (MQ=255)
                                                                                                                                                                                                  aTTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGt                                                                                                                                     <  1:156901/137‑1 (MQ=255)
                                                                                                                                                                                                  aTTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGt                                                                                                                                     >  2:156901/1‑137 (MQ=255)
                                                                                                                                                                                                       aTCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTTCACGCCGCTCAACAGATTGCCCAACGCGCAGGGGGCCAACAATGAATGGCACCACCTTCGAAAACTGAATTAAGTGCACCGTCTGCACCACCGCCTGCCCCGTTAGCCGGGTGGATCCCGGGTATCCCAGGCCAAAACAAGCCGGGCCGGTAGGGGAGCGCCGGCGGTTGAAAAAGGGGGAACTggaag                          >  2:13997/1‑235 (MQ=255)
                                                                                                                                                                                                           cgtCGCCCAGGGAGGCGGCGGCGGTGTTTGTGCCGTAAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGGAGGAGGCCAACAATGAATGCCACCAGCTCCGAAAACTGCATTAAGTGCCCCGTCGGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGa                      <  1:748406/237‑1 (MQ=255)
                                                                                                                                                                                                            aTCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCCTTAAGTGCACCGTCTGCACCACCGCCTGCCCGGGGAGCCGGGTGAATCCCGGTTATCCAGGGCCACAACAAGCCGGGCCGGATGGCGCGCGCGTGCGTTTGTACGATGGCGCACTGTATGACCAg                     >  1:902039/1‑239 (MQ=255)
                                                                                                                                                                                                            aTCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGAGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCCGGCCGGATGGCGAGCGGCTGCGTTTGAAAAATGGCGCACTGTATGa                         >  1:236285/1‑235 (MQ=255)
                                                                                                                                                                                                               gccaggggACGCGGGGGCGGGTGTTATACCGTAGGTGCTTTACATGCCGCTCAACAGTTTGCCCAACGCGCAGGAGGCCAACAATGAATTACCCCAGCTTCGAAATCTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGgcg                  <  1:472222/234‑1 (MQ=255)
                                                                                                                                                                                                                          cggcggcggTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTTAAAGATGGCGCACTGTATGCCGAGGCGCTGAAATAttt       >  1:580118/1‑238 (MQ=255)
                                                                                                                                                                                                                          cggcggcggTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAATGCATTAAGTGCACCGTCTGCACCAACGCCTGCCCGGTGAGCCGGGGGAATCCCGGTTATCCAGGGCCAAAACCAACCGGGCCGGCTGTGGAGCGGCCGCGGTTTAAAAATGGGGCACTGTATGACGAGGCGCCGAAATAtt        >  2:385209/1‑238 (MQ=255)
                                                                                                                                                                                                                               gcggTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTTTGACCAGGCGCTGAAATATTGCATCa  >  1:17333/1‑239 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
CGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGGCGCTGAAATATTGCATCA  >  NC_000913/2355231‑2355692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: