Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,936,843	T→C	15.0%	S420S (TCT→TCC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,843	0	T	C	15.0%	142.5 / 2.6	64	S420S (TCT→TCC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (32/19);  new base C (4/6);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAA  >  NC_000913/2936610‑2937075                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          ggCGTGGAAATGTTAATGGGGCACCAGCTCCCCGGCCCCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCa                                                                                                                                                                                                                                      <  2:855707/239‑1 (MQ=255)    gTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTTGAAACAACCCGCAAGCGAAGGGAACCCCACGATAAAACCAAACAGGGAAAACTCAcaacaaag                                                                                                                                                                                                                                   >  2:387403/1‑237 (MQ=255)      ggCAATGCTAATGGGTCACCCGCTCACCGGCCCCGCTCCGGTCTTTGCCGCTGTTCGTACCTACTGGTCACCAGAAGCAATTGGGCGTGTAACCGGGCATAACCTTGATTGACTGGCAGCACACGGCATCCTCCCATTTCTCAACTCCGCTTTCGCTGCGCTGGACGGGTTCCGTAAACTCACCACCACCGAAGGGAACCCAACTATCGAGCCACACTGGGAAACCCCCCCCCAAGAgg                                                                                                                                                                                                                                 >  2:8009/1‑239 (MQ=255)                       aCCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcgc                                                                                                                                                                                                                 >  1:869591/1‑238 (MQ=255)                       aCCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAAAGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGAAAAAGGCTGACCCTTGGCTcgc                                                                                                                                                                                                                 >  2:19873/1‑238 (MQ=255)                       aCCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCGGCGCTGGACGGGTCCTGTAACCAACGCGACACCGAAGGTAACCCAACGATGAAGCCACACTGGGAAATATCTCAGCAAAGGGCTGACCATTCCCGcgcc                                                                                                                                                                                                                >  1:774970/1‑239 (MQ=255)                                       cGCTCAGGTATTTGCCGGTGGGCGTACCTTCTGGCCCCCAGAAGCAATTGAGCGTTTAACGGGGCATAAACTGGATGGACTGGCAGAACCCGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGcc                                                                                                                                                                                                <  1:19873/239‑1 (MQ=255)                                                aTTTGCCGATGTGGGTCCCTACTGGCCACAAGAAGCAATTGGGGGTGTAACGGGGCATAAACTGGAGGGCCTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGGGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCCCAGCAAGAGGCTGACGTTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATcc                                                                                                                                                                                       <  1:744931/239‑1 (MQ=255)                                                 tttGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAAGGTGCCCAGCGATCCa                                                                                                                                                                                      >  1:119551/1‑239 (MQ=255)                                                   tGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCACCAAGAGGCTGACGCTTGCCTCGCAGCTACCGAATGGTGCCCGGCGAtacccg                                                                                                                                                                                    >  1:748766/1‑234 (MQ=255)                                                     ccGAGGTGCGTACCTACGGGCCACCAGAAGAAATTGAGCGTGTAACGGGGCAAAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGaa                                                                                                                                                                                  <  1:387403/239‑1 (MQ=255)                                                               tACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCCCGAATACTTCCGTg                                                                                                                                                                        >  1:456270/1‑239 (MQ=255)                                                               tACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTCCCGTg                                                                                                                                                                        >  1:713294/1‑239 (MQ=255)                                                                 cctggtGGGCACCAGAAGCAATTGGGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTggc                                                                                                                                                                      <  1:703986/234‑1 (MQ=255)                                                                 cctcttGGTCCCCAGAACGAATGGGGCTGGTAACGGGGGATAAACGGGGGGGGCGGGCAGAACACGGACTCATCCATTTGGTCAACGCCGGTTTGGCGGCGCTGGACGGTTCCGGTAAACAACGCGACAGGAAAGGTACCCCGACGATGAAGCCACTCTGGGAAATCTCCCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTggc                                                                                                                                                                      <  2:578600/234‑1 (MQ=255)                                                                  cTACTGGTCACCAGAAGCAATGGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACCCGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGAAAACAACGCGACAGCGAAGGTACCCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTggc                                                                                                                                                                      <  1:899599/238‑1 (MQ=255)                                                                     cTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGCAAGCCAAGGTAACCCGACGATGAAACCACACCGGGAAAAATCCCAGCAAAAGGCTGCCGCTTGCCTCCCCGCACACGAATGGAGCCCGCGGAGCCACGAAAACTACCGgggggggg                                                                                                                                                                  >  2:136444/1‑237 (MQ=255)                                                                     cTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGGGgcggcg                                                                                                                                                                  >  1:680980/1‑239 (MQ=255)                                                                       ggTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGGGGCGGCGGt                                                                                                                                                                >  1:680372/1‑239 (MQ=255)                                                                       ggTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAAAGCGACAGCGCAGGTAACCCGGCGATGAAGCCACACTGGGAAATATCCCCGCAAGAGGCTGACGCTTGCCTACCCGCTCCCGAAGGTTGCACGGCGATCACCGAAACCTTCCTGGGCGGCGGt                                                                                                                                                                >  2:628347/1‑239 (MQ=255)                                                                           aCCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCCCACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAAGGTGCCCGGCGATCCCCGAATACTTCCggggggggggggaa                                                                                                                                                             >  2:899599/1‑234 (MQ=255)                                                                            ccAGAAGGAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAag                                                                                                                                                                                                                                    <  2:437165/166‑1 (MQ=255)                                                                            ccAGAAGCAATTGGGGGTTTAACGGGGCATAAACTGGATGGACTGGCAGAACCCGGCATCATCCATTTGACCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTActc                                                                                                                                                           <  1:578851/239‑1 (MQ=255)                                                                            ccAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAag                                                                                                                                                                                                                                    >  1:437165/1‑166 (MQ=255)                                                                                    aaTTGGGCGTGTAACGGGGAATAAACGGGGTGGACTGGCAGAACCCGGCATCCTCCATTTGATCAACTCCGGTTCGGCTGCTCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCt                                                                                                                                                   <  1:58719/239‑1 (MQ=255)                                                                                    aaTTGGGCGTGGAACGGGGAATAAACGGGATGGATTGGCAGAACACGGCATCATCCATTTGAACAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCg                                                                                                                                                     <  1:53962/237‑1 (MQ=255)                                                                                     aTTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTAGCCCCTACCGAATGGTGCCCGGCGATCCACGAAAACTTCCGGGGCGGCGGTTCCTCTTCCCGcc                                                                                                                                                   >  1:432625/1‑237 (MQ=255)                                                                                       tGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcct                                                                                                                                                  >  1:536566/1‑235 (MQ=255)                                                                                           cGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTTCCTCGCCCCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGGGCCGGCGGTTACTCTTCCCCCCTCCTTa                                                                                                                                             >  2:650158/1‑238 (MQ=255)                                                                                           cGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCCAAGCAAGAGGCTGACGCTTGCCTCCCCGCTACCGAATGGTGCCCGCCGATCCACGAATACTTCCCTGGCGGCGGCTCCTCTTCCCCCTCCCTTa                                                                                                                                             >  2:410599/1‑238 (MQ=255)                                                                                             tgtAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTAACGCTTGCCTCGCCGCTACCGAATGTCGCCCCGCGATCCACGAATACTTCCGTGGAGGCGGGTACTCTTTCCCCTTCCTTACCg                                                                                                                                          >  1:302590/1‑239 (MQ=255)                                                                                                   ggggCATAAACTGGATGGACTGGCAGACCACGGCATCATCCATTTGACCAACGCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAggcg                                                                                                                                    <  1:299410/239‑1 (MQ=255)                                                                                                     ggCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGCAATCTCTCAGCAAGAGGCTGCCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGGTACACTTCCCGCTTCCTTTCCGCAggcggc                                                                                                                                  >  1:147125/1‑239 (MQ=255)                                                                                                       cATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAGAACTTCCGAGGCGGCGGTTATCCTTCCCCCGTCCTTTccacagggcggg                                                                                                                                 >  2:99734/1‑228 (MQ=255)                                                                                                                gatggaCTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGttta                                                                                                                        >  1:25384/1‑236 (MQ=255)                                                                                                                 atggaCTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGCTACCCTTCCCCCCTCCTTACCCAAAGCGGCGCCCCGTGCAcc                                                                                                                      >  2:740150/1‑239 (MQ=255)                                                                                                                      cgggCAAAACCCGGCACCACCCATGTGACAAACTCCGGTTGCGCTGGCGTGGAGGGTTCCGTAAAACAACGGGACAGGGAAGGTAACCCGAGGAGGAAGCCACACGGGGAAATCTCCCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGa                                                                                                                  <  2:536566/236‑1 (MQ=255)                                                                                                                       tGGCAGAACAGGGCCTCACCAATTGGACCAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCGGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACt                                                                                                                <  1:331077/239‑1 (MQ=255)                                                                                                                          cAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGCGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCTACCCGTTcccccttgaccgg                                                                                                             >  1:715101/1‑230 (MQ=255)                                                                                                                               cacGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGCTACCCCTCCCGCTTCCTTCCCGGAGGCGGCGGCCCCGTCCCCATGACCCGGGTcca                                                                                                        >  2:651782/1‑237 (MQ=255)                                                                                                                                   gCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGGTCACCCTGACTCGTTGCC‑Acatc                                                                                                   >  1:58786/1‑239 (MQ=255)                                                                                                                                        ctcCATTTGATCAACCCCGTTTCTGCCGCGCTGGACGGTTCCTGAAAACAACGCGACAGGGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGATGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCaa                                                                                              <  1:136444/238‑1 (MQ=255)                                                                                                                                         tcCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGCAGCCGGAGTCCCGTTCCCCCTGAATCGCGTCA‑Acatctcccaa                                                                                             >  1:343405/1‑233 (MQ=255)                                                                                                                                              ttGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCAAAACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGGTCCACGACTACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCAGCGTCCCGTTTACCCTGACTCGTGTCA‑Aca                                                                                                     >  1:66502/1‑226 (MQ=255)                                                                                                                                              ttGATCAACCCCGGTTCTGCTGCGCTGGCCGGTTCCTGAAAAAAACGCGAAAGGGAAGGTACCCCGACGATGCAGCCACCCTGGGAAATCTCCAAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCt                                                                                        <  1:422515/239‑1 (MQ=255)                                                                                                                                                gATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGCTACTCTTCCCCCCTCCTTACCCAAGGCGGCCTCCCGGTCACCATGACTCGTGTCA‑ACACACTCAAAGGCCTgg                                                                                      >  1:520003/1‑239 (MQ=255)                                                                                                                                                 aTCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAACCAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcgc                                                                                                                                                                                                                 <  1:59637/116‑1 (MQ=255)                                                                                                                                                 aTCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcgc                                                                                                                                                                                                                 >  2:59637/1‑116 (MQ=255)                                                                                                                                                   cAACTCCGGTTCTGCTGGCCTGGACCGTTCCTTTAAACACCGCGAAGGGGAAGGTACCCCGACGATGAAGCCCCCCTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGAc                                                                                   <  1:504637/239‑1 (MQ=255)                                                                                                                                                   cAACCCCGGTTCTGCTGCGCGGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGAc                                                                                   <  1:503214/239‑1 (MQ=255)                                                                                                                                                     aCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCCCGAATACTTCCGTGGCGGCGGTTCCTCTTCCCGCTTCCTTACCCAAGGCGGCGTCCCGTTCACCCTGACTCCGTCCA‑ACACCTTCAAAGGCCTGGGGCCg                                                                                 >  1:449202/1‑239 (MQ=255)                                                                                                                                                                     gcgggACGGTTCCTGTAAACAACGCGACAGCGAAGGGAACCCGGAGATGAAGCCACACTGGGAAATCTCACAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                                  <  1:486075/235‑1 (MQ=255)                                                                                                                                                                              ttCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCAcc                                                                                                                      >  1:634108/1‑178 (MQ=255)                                                                                                                                                                              ttCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGACTGACGCTTGCCTCGCCGCTACCGAATGGAGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCCCTTCCTTACCCAATGCCCTCTCCCGGTCCCCACGA‑ACCCGTCACACACCCTCAAATGCCCGGGACCCGTCCCGCAAACCGCGGAAGACGGGa                                                        >  2:360748/1‑239 (MQ=255)                                                                                                                                                                                ccTGTAAACACGCCGACAGCGAAGGTACCCCGACGATGAAGCCACACGGGGAAATCCCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGc                                                      <  1:628347/239‑1 (MQ=255)                                                                                                                                                                                   gTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTg                                                   >  1:603894/1‑239 (MQ=255)                                                                                                                                                                                      aacaacGCAACAGGGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                                <  1:99734/239‑1 (MQ=255)                                                                                                                                                                                                    gagggTACCCCGACGATGAAGCCACATTGGGAAATCTCTCACCAAGAGGCTGACCCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                                    <  1:569274/234‑1 (MQ=255)                                                                                                                                                                                                          aaCCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGa                            <  1:740150/239‑1 (MQ=255)                                                                                                                                                                                                                        gCCACACGGGGAAATCTCCCAGCAAGAGGCTGACGCTTCCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACaaa               <  1:764167/238‑1 (MQ=255)                                                                                                                                                                                                                           cccggtggAAATCTCGCCGCAGGGGGCGGACCTCTGCCTCGCCCCTACCGAAGGGGGCCCGGCGATCCACGAATACTTCCGTGGGCGCGGTTACTCTGCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCa           <  2:343405/233‑1 (MQ=255)                                                                                                                                                                                                                                gggAAACCCCCCAGCAAGCGGCCGACGCTTGCCTCGCCGGTACCGAATGGTGCCCGGCGATCCACGAATCCTTCCGTGGCGGCGGTTTCTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAAc      <  1:233249/239‑1 (MQ=255)                                                                                                                                                                                                                                 ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGGCCCGTTCACCATGACTCGTGTCC‑ACATCATCCAAGGCCCGGGACCGGGACCGCGAATCCCCGAAGGCTGGAGCGTGGAATTGCCCAAGGAGGTGACTCACAACTCCAACAAAAGCACCAAc      >  2:492565/1‑238 (MQ=255)                                                                                                                                                                                                                                    aaTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCCAATCGCGGAAGGCTGGAGCGGGGAATTGCCGAAGGAATTGCATGACATCCTCAACAAAAGCACCAACTCa   >  1:209006/1‑238 (MQ=255)                                                                                                                                                                                                                                       ctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGGACGGTACTGCAAATCCCGGAAGGCTGGAGCGTGGAATTGCCTACGGATGGGCATGACATCCCCAACAAACGCACCAACTCaa  >  1:197269/1‑236 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          GGCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCA‑ACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAA  >  NC_000913/2936610‑2937075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑