Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,053,585 T→G 30.3% N419T (AAC→ACC)  pepP ← proline aminopeptidase P II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,053,5850TG30.3% 69.7 / 5.3 39N419T (AAC→ACC) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (3/23);  new base G (11/2);  total (14/25)
Fisher's exact test for biased strand distribution p-value = 1.37e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGCGTTCT  >  NC_000913/3053349‑3053816
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
cAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGgtt                                                                                                                                                                                                                                        >  1:501796/1‑239 (MQ=255)
          ccgccAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGCTTTCTTCCGGCTTTTTCACCCCCC‑TGGCGGTGAGGGTTTCGTTTCCgg                                                                                                                                                                                                                              >  1:84966/1‑239 (MQ=255)
                 cgcTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGACAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATGTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCGTTACCGGGTTCggg                                                                                                                                                                                                                       >  1:919509/1‑238 (MQ=255)
                 ccctaccccTCGCCCATAAAAGCCCGGATGGGCATGGGACTCTGGCGCAGCCGCTTCAACCAAATGTCCCGGCAGCGCCCCGTGACTTACCCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGt                                                                                                                                                                                                                       <  1:227172/231‑1 (MQ=255)
                           cGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTCGGGGTTTCGTTACCCGGTTTGGGAATTACaaag                                                                                                                                                                                                             >  1:788982/1‑237 (MQ=255)
                              ccATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGGGCCCGCCATGCCGTCACCGACGAGGATTACGCTCATTGCTTTCTCCCAGCAACCCTAAACGCTTCGATTTATTCCGGCTTTTTCACCACGC‑TGGCGGTAGGGGTTTCTGTTCCGGGTTGGGGAAACACAAAgtcg                                                                                                                                                                                                          >  2:626750/1‑239 (MQ=255)
                              ccATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCACCGCCAGCGTCCCGCCCGCCATGCCGCCACCGCCGATGATTACACTCATTGCTTTCTCGCACCAACCATCAACGCTTCGCTTTCTTCCGGCTTTTTCACCCACC‑TGCGGGGGAGGTTTTTCCTCCCCGTTTGCGTAAACACaaaatac                                                                                                                                                                                                          >  2:475066/1‑234 (MQ=255)
                                                 cATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCCTTACCGGGTTTGGTGATGCCCATTTCGCCTTCCATActatagcc                                                                                                                                                                                         >  1:835729/1‑230 (MQ=255)
                                                      gcctctGGCGCAGTCCCTCAAATCAAATGTCCCGGCAGCGCCCCGGGACTTAACCGGGAAATAGCCAGCGCCGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcg                                                                                                                                                                                  <  1:62435/237‑1 (MQ=255)
                                                                       tttaatcaaATGTACAGGCAGCGCCCCGTGACTTCACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑Ca                                                                                                                                                                 <  1:412330/235‑1 (MQ=255)
                                                                               aaTGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGGGAGGGTTTCGTTTCCGGTTTCGGGAATCACAATGTCGGCTTCCATACGAATGCCGATACCCCGGCATTGTTCTTG‑CCCTTCTGCa                                                                                                                                                         >  1:473466/1‑238 (MQ=255)
                                                                                tgtcaCCGGCGGCCCCCCGTGACTAAACCGGGAAATGGCCAGCGCCGGCGTCCCGCCCGCCATGCCGCCCCCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATc                                                                                                                                                       <  1:523270/235‑1 (MQ=255)
                                                                                attccccGGCCGCCCCCCGTGACTGACCGGGGAAAGAGCAAGCGCCACGCTCCCCTCCGCCATGCCGCCGCCGACGATGGTTCCGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATc                                                                                                                                                       <  1:857189/234‑1 (MQ=255)
                                                                                  gccccggacgcgcCCCGTGACTTAACCGGGAAATAGCCAGCCCCAGCTGCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCg                                                                                                                                                     <  1:713827/230‑1 (MQ=255)
                                                                                      cGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCGTTACCCGTTTCGGTAATCACAAATTCGTCTTCAATACGAAATCCGCTACCGCGATATTGTTTTGGCCACTCCT‑CATCCGgccc                                                                                                                                                 >  2:442437/1‑237 (MQ=255)
                                                                                            cgcCCCGTGTCTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAatat                                                                                                                                            <  1:244160/238‑1 (MQ=255)
                                                                                            cgcCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑CGGCGGTCAGGGTTTCGTTACCGGTTTCGGAAATCACAATGTCGCCTTCAATACGAATACCGATACCACGCTATTGTTTTGG‑CACTTCTTCCTCCCCCGCAAaata                                                                                                                                           >  2:110239/1‑239 (MQ=255)
                                                                                                         aaCCGGAAAATGGCCAGCGCCCGGGTCCGGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                                                                                                              <  1:579281/239‑1 (MQ=255)
                                                                                                                         gcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTTAATTCGAATGCCGATACCGCGTTATTGTTCTGG‑CACTTTTGCATCCGGCGCAATATCAATCCCTGGCTCCACGGGTCGTACCATg                                                                                                               >  1:101952/1‑238 (MQ=255)
                                                                                                                                  ccgccccccgcATGCCGCCCCCGACGATGATTACGCTCTTTGCTTTCCCCGAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCa                                                                                                     <  2:101952/229‑1 (MQ=255)
                                                                                                                                                           gatgatTAAGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATa                                                                            <  1:602262/239‑1 (MQ=255)
                                                                                                                                                           cattgttACGCTAATTGCCTTCCCGCAGCAACCATCACCGCTTCCATTTCCTCCGGCTTTTTCCCCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATa                                                                            <  1:626750/234‑1 (MQ=255)
                                                                                                                                                                 tACGCTTCTTGCTTTCTCGCAGCAATCATCAACGCTTCGATTTCTTCCGGCCTTTTCACCACGC‑TGGCGTTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACGTCTGCATCCGGCGCAATATACAGCCCTGGCTCGACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACAcc                                                                      <  1:403241/239‑1 (MQ=255)
                                                                                                                                                                 tACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACAcc                                                                      <  1:277098/239‑1 (MQ=255)
                                                                                                                                                                  tcGCTCATTGCTTTCCCGCAGCAACCATCAACGTTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACAccc                                                                     <  1:835231/238‑1 (MQ=255)
                                                                                                                                                                          tGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATg                                                             <  1:506673/239‑1 (MQ=255)
                                                                                                                                                                                cccGCAACACCCTTCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCATCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATc                                                       <  1:821260/237‑1 (MQ=255)
                                                                                                                                                                                            tgcAACGCTGCAGTTTCGTCCGGGTTTTTCACCACGC‑TGGCGGTGATGGTTTAGTTACCGGTTTCGGTAATCACAATGGCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCTCAATATGCAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCa                                           <  1:397139/237‑1 (MQ=255)
                                                                                                                                                                                            cacAACGTTTCGATTTCGTCCGGCTTTTTCGCCCAGA‑TGGCGGTGAGGTTGTCGTTACCGGTGTCGGTAATCACAATGTCGTCTTAAATACGAATGCCGATACCGGGATATTGTTCTGG‑CACTTCTGCAGCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCa                                           <  1:67995/237‑1 (MQ=255)
                                                                                                                                                                                            atgtctgCTTTGTTTTTTTCCGGGTTTTCCACC‑CGCGTGGGGGGGAGGTTTTCGTTCCCTGTTTCGGTAATCAACATGGCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCa                                           <  2:275623/233‑1 (MQ=255)
                                                                                                                                                                                            aTCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCa                                           <  1:673435/239‑1 (MQ=255)
                                                                                                                                                                                                  gCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGTGTTTCCTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCt                                     <  1:165558/239‑1 (MQ=255)
                                                                                                                                                                                                   cTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAACGCGATATTGTTCTGG‑CACTTCTTCATCCGGCGCAATATACAGCCCCGGCTCTACGGTCAGTACCATGCCCGGGTCCAGAAAGCGCGAGCGATCCTGTCCCTTAAAAACCCCGGCATGGACATCCAAGCCCAACCaaggggct                                    >  2:49102/1‑232 (MQ=255)
                                                                                                                                                                                                   cTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAAGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAACCCCGGCTCTACGGTCAGTACCATGCCCCGTTCCAGAATGCGCGAGCGATCCTGCCCATAAAAACCCACGCCATGGAAATCCAGt                                                   >  1:748455/1‑224 (MQ=255)
                                                                                                                                                                                                    ttCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCCCAATTTCGTCTTCAATACGAATGCCGCTACCGCGATTTTGTTATGG‑ACCTTCTCCATACCGCGCAATTAACAGCCCTGACCCTCCCGTCCGTACCATTCCCGGGTCCCGAAAGCGCGCGCGGTCCTGACCATAAACACACACGCCCTGGACAACCAGGCCTAACAAAAGGCTaa                                   >  2:918726/1‑239 (MQ=255)
                                                                                                                                                                                                       gATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGc                                <  1:442437/239‑1 (MQ=255)
                                                                                                                                                                                                             ttcCGGCTTTTTCACCACGG‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACCAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCa                          <  1:49102/239‑1 (MQ=255)
                                                                                                                                                                                                              tcCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCCCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCCTAAACACCCACGTCATGGACATCCAACCCTAACCAATGGCTAAGGCCATGcct                         >  1:746654/1‑237 (MQ=255)
                                                                                                                                                                                                              ccgctcTTTTTC‑CCCCGG‑TGGCGGTGAGGTTTTGCTTACCCGTTTCGGTAAGCATAGTGTCGGTGTCAATCAGAATGGCGATGCCGGGATATTGTTCTGG‑CACTCCTGCATCCGGCGCAATATACAGCCCTGGCACTACGCTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATa                        <  2:777837/234‑1 (MQ=255)
                                                                                                                                                                                                                                       gtgtGGTTTTCGCTACCTGTTTCGGCAAGCACAATGTCGGCTTCAGTACGAATGCCGATACCGCGATATTGATCTGG‑CACTTCTGCAACCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGCGTTCt  <  1:159957/238‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
CAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGC‑TGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGG‑CACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGCGTTCT  >  NC_000913/3053349‑3053816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑