Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,293,687	T→C	17.9%	A96A (GCT→GCC)	lpoA →	OM lipoprotein stimulator of MrcA transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,293,687	0	T	C	17.9%	130.2 / 5.7	71	A96A (GCT→GCC)	lpoA	OM lipoprotein stimulator of MrcA transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (27/28);  new base C (5/9);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGA  >  NC_000913/3293478‑3293916                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         tgtgGCCCCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGt                                                                                                                                                                                                                           <  1:683475/239‑1 (MQ=255)  ttgGCACCCATGCTCCCGATCAGTCCACTGCGTATATGCAGGGCAAGGCGCAGGCTGATGCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTATGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  1:873904/238‑1 (MQ=255)  gtgGCACCCATACTCCCGATCAGGCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTa                                                                                                                                                                                                                          <  1:487691/239‑1 (MQ=255)               tCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑Tgg                                                                                                                                                                                                        <  1:155652/239‑1 (MQ=255)                   gATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTTAG‑TTTTTTAACCCACTACCGCAAAAACTGAACGAAGGCCAGC‑GTC‑GGGCAAAAAAACCTCTGGGCGGA‑AAGA‑TTAA‑AAC‑TGgc‑ga                                                                                                                                                                                                    >  2:71772/1‑238 (MQ=255)                           cACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑CTGTTTAACCAACTACCGCACGAACTTAACGATCGTCAGC‑GTC‑GCGAGCAAACACTGCTTGCCGTA‑GCGA‑TT‑A‑CAC‑TGGT‑GCAGAAAGAt‑t                                                                                                                                                                                          >  1:125414/1‑239 (MQ=255)                                   atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGGG‑TTGTTTAACCACCTACCCCAAGAACTGAACAATGCTCAGC‑GTC‑GCGTGAAAACACTGCTGGCGGGA‑GAGA‑TT‑A‑AAC‑AGGC‑GCAGAAAAAt‑tttttttg                                                                                                                                                                                   >  2:165562/1‑233 (MQ=255)                                   atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAAAG‑Tttgc‑t‑t‑gc                                                                                                                                                                               >  1:676999/1‑239 (MQ=255)                                        cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGCG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCACC‑GTC‑CCGGGAAAACACTGCTGGCGGCA‑AGAA‑TT‑A‑AAA‑TGGG‑GCAAAAAGAA‑TTTGT‑T‑G‑GGGCG‑Ca                                                                                                                                                                         >  1:299326/1‑239 (MQ=255)                                          gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGGG‑TTGTTTAAACAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGGAGGAGT‑TA‑A‑AAC‑TGG‑‑GCGCACAGAT‑TTTGC‑T‑G‑GCGCC‑Caaa                                                                                                                                                                       >  1:236451/1‑239 (MQ=255)                                          gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCCACTACCGCAAGAACTGAACGATGCTCAAC‑GTC‑GCGAGAAAACCATGCTGGCGGCG‑GGGG‑TT‑A‑AAA‑TCGC‑GCGAAAAGAT‑TTTGC‑T‑G‑GCGCG‑Ccaa                                                                                                                                                                       >  1:28903/1‑239 (MQ=255)                                          gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAAATACCGCAAAAACTGAACGATGCTCAGG‑GTC‑CGGGGAAAAAACTGCTTGGGGTA‑GAGA‑TT‑T‑AAA‑TGGG‑CCGCGAAAAT‑TTTTC‑T‑G‑GGGCG‑Caaa                                                                                                                                                                       >  1:653413/1‑239 (MQ=255)                                           ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCCAGAACTGAACGATGCCCAGC‑GTC‑GCGGGAAAAAACCCCTGGGGGCA‑GAGA‑AT‑A‑AAC‑TGGC‑GCAGAAAAAC‑ATTGC‑T‑G‑gcggcccaaa                                                                                                                                                                       >  2:61104/1‑232 (MQ=255)                                           ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGGA‑AGAA‑TT‑A‑AAA‑TGGC‑GCGAAAAAAT‑TTTGC‑TGG‑GCGCG‑Caa                                                                                                                                                                        >  1:685888/1‑238 (MQ=255)                                           ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑CCGCGAAAACACTGCTGGCGGGA‑GAGG‑TT‑T‑AAC‑TTGC‑GCCGAAAAGT‑TTTGC‑T‑GTGCGCC‑Caaa                                                                                                                                                                       >  1:72841/1‑239 (MQ=255)                                               cGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCCAGAACTGAACGATGCTCAGC‑GTC‑GCGAGGAAACACTGCTGGCGGTG‑AGGG‑TT‑A‑AAC‑TGGC‑GCCGAAAGAT‑TTTTG‑T‑T‑GAGCG‑CCAAACTTg                                                                                                                                                                  >  1:711115/1‑239 (MQ=255)                                                ggCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCCCAGC‑GTC‑GCGCGAAAAAACAGCTGGCGGTA‑GAGATTT‑A‑AAC‑TGCG‑ACAAAAAAAT‑TTT‑C‑T‑G‑GGGCG‑CAAAACttcc                                                                                                                                                                 >  2:375590/1‑237 (MQ=255)                                                  cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGC‑GTC‑GCGAGAAAACACCGCGGGCGGGA‑GAGA‑GT‑A‑AAA‑ATGC‑GCGGAAAAATATTTTC‑T‑G‑G‑GCG‑Caaaaaatggtg                                                                                                                                                               >  2:706230/1‑232 (MQ=255)                                                            ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTTGTTTACCAACTACCGCCAAAACTGAACCATGCCCACCGGCC‑GCG‑GAAAACACTGCCGGCGGAACGGCA‑TA‑A‑AACGTCGC‑CCA‑AAAGAT‑TTTG‑‑T‑G‑GCCCG‑CGAAAATTGCTGGGGacaaaaa                                                                                                                                                     >  2:398300/1‑236 (MQ=255)                                                            ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAAATGGACGATGCTCTGC‑GGC‑GCGGGAAAACACTGCTTGCGGGA‑GGAA‑TT‑T‑AAA‑TGGG‑GCAGAAAGAT‑GTTTC‑T‑G‑G‑GCGCCAGAACCTGCTGGCGGAAAAca                                                                                                                                                     >  2:139823/1‑239 (MQ=255)                                                                 ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGGGAAAACAATGCTGGCGGTA‑GGGA‑TT‑A‑AAC‑TGGC‑GCCACAAGAT‑TTTGC‑T‑T‑GCGCG‑CAAAAATTTCTTGCGcaaaaccaacc                                                                                                                                                 >  1:846378/1‑231 (MQ=255)                                                                 ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGCCGGTTGAG‑TTGTTTAACCAACTACCCGCAGAACCGAACGATGCTCAAC‑GGC‑GCGAGAAAAAACTGCTGGCGGT‑‑GAGAGTT‑G‑AAC‑TGGC‑GCCGAAAAAT‑TTTTCGT‑G‑GCCGG‑CAAAA‑TTGCTGAGGaaaaaaaaaac                                                                                                                                                 >  2:74994/1‑231 (MQ=255)                                                                  cTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAAATACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTCGCGGTA‑GAGG‑TT‑A‑AAC‑AGGC‑GCAGAAAAAA‑TTTGC‑T‑G‑GGCGG‑CAAAACATGCTGGGCAAAATcaacact                                                                                                                                                >  1:769897/1‑233 (MQ=255)                                                                   ttttATTTTCAGCAGATGCAACAAGGTTTTGATGATACCAGGAACAACGGGCAATTCCCCGCCATTCGTGCACTGGGAAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCGGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                              <  2:7938/239‑1 (MQ=255)                                                                             cggagATGCAACAAAGTTTTGATGATCCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGaa                                                                                                                                  <  1:568320/236‑1 (MQ=255)                                                                                  aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAC‑GAAGGTAAAACCGGGCAGGCGGTTTAG‑TTTTTTAACCAGATACCCCAAGAAATTAACGATGCCCAAC‑CTCGGCGA‑AAAACCCTGCGCGCGGGG‑GAGA‑AT‑ACAAC‑GGGC‑GCAAAACGTA‑TTTCT‑G‑G‑GCGGC‑CAAAACTACTGGGCGAAA‑‑CACACCCACC‑GCATTTAGAaaaaaa                                                                                                                             >  2:330262/1‑239 (MQ=255)                                                                                            gCTCTGATGATACCAGGTACAATTGGCAATTGCTCGTCATTTGTGAACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAgcg                                                                                                                   <  2:192851/239‑1 (MQ=255)                                                                                             ctctGATGATGCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑Attt                                                                                                                                      <  1:611380/219‑1 (MQ=255)                                                                                             ctctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGGA‑GAGA‑TT‑A‑AAC‑TGGG‑GCAGAAAAAT‑TTTGC‑T‑G‑GGGCG‑CAAAACTTGCTGGCGAAAATTACACCTGCC‑G‑ATTTAGAACAAAACCACCaaaccc                                                                                                                  >  1:804232/1‑235 (MQ=255)                                                                                                      tcccAGGATCAGCGGGCAATTACTCGACATTCGTGCAATGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTGGAACAAAACCAGCAAGCGCGTTACTgg                                                                                                          <  1:180550/236‑1 (MQ=255)                                                                                                      tcccAGGATCAACTGGCAATTACGCGCCATTCGTGCACTGGGGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTgg                                                                                                          <  1:165562/236‑1 (MQ=255)                                                                                                       aCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCCGC‑GTC‑GCGAGAAAACAATGCTGGCGGGA‑GAGA‑TT‑A‑AAA‑TGGCGGCGCACAAAT‑TTTTC‑T‑G‑G‑GCG‑CCAAACCTTCGTGCGGACAACACCACCGCC‑C‑ATTTTGAAAAAAAACAAACAGCGCGTTAATgg‑ca                                                                                                       >  2:919038/1‑239 (MQ=255)                                                                                                                 aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGCTGCTCAGC‑GTC‑GCGGGAAAACACTGCCGGCGGTA‑AAGA‑TT‑A‑AAC‑GGGG‑GCAGAAAACA‑TTTGC‑T‑G‑GGGCG‑CAAAACTTGCCGGCGAAAATAACACCCGCC‑G‑ATTTAGAACAAAAAAAACAAGCACGTTAACGG‑AAGG‑Caaaaaaa                                                                                            >  2:259804/1‑237 (MQ=255)                                                                                                                 aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGG‑TT‑A‑AAC‑TGGC‑GCAGAAAAAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGGAAATTACACCTTCC‑G‑CTTTAGAACAAAACCAGCAAGCGCGTTACTGGCCAGC‑Caaaaac                                                                                             >  1:717734/1‑238 (MQ=255)                                                                                                                     gCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAAAT‑TTTTC‑T‑G‑GCGCG‑CAAAACTTGCTGGGCAAAATCAAACCTTCC‑C‑AATTTGAACAAAAACAGCAAGCCGGTTAATTG‑CAAG‑Ccaaaaaagct                                                                                         >  1:280560/1‑233 (MQ=255)                                                                                                                     gCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCGCAGC‑GTC‑GCGAGAAAACAACGCTGGCGGGA‑GAGA‑GT‑A‑AAA‑TGGG‑GCGGAAAGAT‑TTTTC‑T‑G‑GCGCG‑CCAAACATTGTGGGGAAAATAACACACGCCGG‑ATTTGAAAAAAAACCAACAAGCGCGTTAATGG‑AGGG‑aaaaaaaccag                                                                                         >  2:708801/1‑234 (MQ=255)                                                                                                                      caatgcccggCCATTGGTGCCGTGGTAAAA‑AAAGGAAAGACGGGGC‑GGGGCGTGAGTTTGTTAAACCAAGTGCCGGAAAGACGTAACGAAGCCAAGC‑GTG‑CCGAGAAACCACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑GGGC‑GCAGAAAGAG‑TTTGC‑G‑G‑GCGCG‑CAAACCTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                        <  2:28903/229‑1 (MQ=255)                                                                                                                      cAATTCCCCCCCTTTCGTGCCTTGGTGAAA‑GAAGGTAAACCCGGGGTGGGGGTTGTG‑TTGTTTAACCAACTTGCGCAAGAACTGACCGATGCTCAGC‑GTC‑GCGAGAAAACCCTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑AAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                        <  2:676999/238‑1 (MQ=255)                                                                                                                      cAATTACCCGCCATTCGTGCACTGGGGAAA‑GAAGGGAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTAACGCAAGAACTGAACGATGCGCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                        <  1:919038/238‑1 (MQ=255)                                                                                                                      aattttcctgCCTTTCGGGCCCTGGTAAAA‑AAAGGTAAAACGGGGAAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑GGGC‑GCAGAAAGAT‑TTTGC‑G‑G‑GCGCG‑AAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                        <  2:213340/229‑1 (MQ=255)                                                                                                                                 ggttCGTGCGCTGGTGAAA‑AAAGGAAAAACCGGGAAGGCGGTTGAG‑TTGTGTAACCAACTACCGCAAGAACTGAACGATGCCCGGG‑GCG‑GAGAGAAAGCACGGCCGGCGGTC‑GGGA‑TT‑A‑AAC‑AGGC‑GCAGAAAGAT‑TTTGC‑G‑G‑GCGCG‑CAAAACTTGCTGGAGAAAATCACCCCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAgggg                                                                            <  2:404120/236‑1 (MQ=255)                                                                                                                                 cATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAgggg                                                                            <  1:71772/238‑1 (MQ=255)                                                                                                                                  attggggCACTGGTGAAA‑GAAGGTAAACCCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCg                                                                          <  1:51163/233‑1 (MQ=255)                                                                                                                                          cttgggaaaa‑GAAGGTAAAACCGGGCAGGCGTTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTAAGG‑CTC‑GCGAAAAAACGCTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTAC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:38524/232‑1 (MQ=255)                                                                                                                                          attGGGGAAA‑AAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGAAAGAACTGAACGATGCCCAGC‑GTA‑GCGAGAAAACCCTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑AAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:375590/237‑1 (MQ=255)                                                                                                                                          aCTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:781305/239‑1 (MQ=255)                                                                                                                                          aCTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:61104/239‑1 (MQ=255)                                                                                                                                          aCTGGGAAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACGGAACGATGCCCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAAA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:398300/239‑1 (MQ=255)                                                                                                                                           ctgggtaaa‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:376044/232‑1 (MQ=255)                                                                                                                                            atgTGAAAGGAAGGTAAACCCGGGAAGGCGGTTGGG‑TTGTTTAACCAACTACCGAAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCa                                                                  <  1:8438/236‑1 (MQ=255)                                                                                                                                              gTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGG‑GCAGAAAAAA‑TTTGC‑T‑T‑GCGCG‑CAAAACTTGCTGGCGAAAATCACAACTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGGTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCACGGGCCGCCTTCCCtt                                                                >  1:765266/1‑237 (MQ=255)                                                                                                                                                   a‑gaagGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGGA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGGAAATCACACCTGCC‑G‑ATTTAGAAAAAAACCAGCAAACCCCTTACTGG‑CCGGCCAAAAATC‑ATGCCCGCCCAGGGCGGCCCTTCCA‑TGAtttta                                                         >  1:191072/1‑237 (MQ=255)                                                                                                                                                      aagGAAAACCCGGGAGGGGGTTTGAG‑TTTTTTAACCAACTACCGAAAAAATTGACGGAGGTCCAGG‑GTC‑GCGGGAAACCCCTCGTGGGGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGGGAAAATCCCCCCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTg                                                       <  2:685888/239‑1 (MQ=255)                                                                                                                                                         gTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGGA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAACGCGTTACTGG‑CAGG‑CAAAAAACGAAGCCAG‑CCAAGGGGGTCCCTCCCTTGTTTTACTgcgc                                                    >  1:12364/1‑239 (MQ=255)                                                                                                                                                          tAAACCCGGGCAGGGGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:706230/237‑1 (MQ=255)                                                                                                                                                          tAAACCCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:754065/237‑1 (MQ=255)                                                                                                                                                          tAAAACCGGGCAGGCGGTTGAG‑TTTTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  1:853582/237‑1 (MQ=255)                                                                                                                                                          aaaaaCCGGGGGGGGGTTTGAT‑TTTGTTACCCAACCACGGAAAAAACGGAAGGAGGTCAAGG‑GTC‑GCGATAAAACCCTGCGGGCGTGA‑GGGA‑TT‑A‑AAC‑GGGC‑GCAGAAAGAT‑TTTGC‑G‑G‑GCTGA‑CAAAACTGGCTGGCGAAAATCACCCCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTgcg                                                     <  2:236451/236‑1 (MQ=255)                                                                                                                                                                 gggcgggcGTTTGAT‑TTTTTTAACCAATTACCGCAAGAACTGAACGAGTCCCAGG‑GGC‑GCGAAAAAACACGGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACCCCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAAt                                           <  2:12364/239‑1 (MQ=255)                                                                                                                                                                 gggCAGGTGTTTTAG‑TTGTTTAACCAATTACGGAAAGAACTGAAAGAGGCCCAGA‑GCC‑CGGAGAAAACGCCGCTGGAGGTA‑AGGT‑TT‑A‑AAC‑TGGC‑GCAGAAAAGT‑TTTGG‑C‑G‑GCCCG‑AAAAACTTGCTGGCAAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAAt                                           <  2:254283/239‑1 (MQ=255)                                                                                                                                                                         ggTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCCGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAAACTGCTGGCGAAAATCACACCTGCC‑G‑ATTTATAACAAAAACAGCAAAAGCGCTACTGG‑CAGG‑CAAAAATAGATGCCCC‑ACCCGGGCGGCCCTCCATTTGTTTTCTTCGCGCGGTTAAtttccagg                                    >  2:667877/1‑233 (MQ=255)                                                                                                                                                                                    tttAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GGTAATTGCCCAGGAACCgctgct                         >  1:155967/1‑239 (MQ=255)                                                                                                                                                                                          cAACTAACGCAAGAACTGAACGGTTGTCAGC‑GTC‑GGGAGAAAACACTGGTGGCGGTA‑GAAA‑TT‑A‑ACC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑AAAAACTTGCTGGAGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGgcgc                   <  1:430299/239‑1 (MQ=255)                                                                                                                                                                                              tACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CCAAAAACCATGCCCG‑CCAGGGGCGGCCTTCCCTTATGTTACTGCGCCC‑GTTAATTGCTCACGCACCGCTGCCTGGCGCgaaa               >  2:141049/1‑239 (MQ=255)                                                                                                                                                                                              tACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCCG‑CTTAATTGCTCCGGAACCGCTGCTTGGCGCgaaa               >  1:182826/1‑239 (MQ=255)                                                                                                                                                                                                    aaGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAACCCCTGCGGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAAAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCAAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa         <  1:679685/239‑1 (MQ=255)                                                                                                                                                                                                       aaCTGAACGATGCTCAGC‑GTC‑GCGAGAAAACCCGGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag      <  1:809938/239‑1 (MQ=255)                                                                                                                                                                                                        aCTGAACGATGCTCAGC‑GTC‑GCGAGAAAACCCTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGTT‑TTTGC‑G‑G‑GCGCC‑CAAAACTGGGTGGAAAAAACCACCCCTGAC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag      <  2:72841/238‑1 (MQ=255)                                                                                                                                                                                                        aCTGAACGATGCCCAGC‑GTC‑GCGAGAAAACCCTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑GAAAACTTGCTGGCG‑AAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagca    <  1:627783/239‑1 (MQ=255)                                                                                                                                                                                                          ttaaCGAGGCTCAGG‑GTC‑GCGAGAAAACACTGCGGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCCCG‑AAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag   <  1:100366/237‑1 (MQ=255)                                                                                                                                                                                                           gAACGATCTTCAGC‑GGC‑GCGAGAAAACACGGGCGGCGGTG‑AAAA‑TA‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCGGGCGAAAATCCCACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGa  <  2:711115/239‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA‑GAAGGTAAAACCGGGCAGGCGGTTGAG‑TTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC‑GTC‑GCGAGAAAACACTGCTGGCGGTA‑GAGA‑TT‑A‑AAC‑TGGC‑GCAGAAAGAT‑TTTGC‑T‑G‑GCGCG‑CAAAACTTGCTGGCGAAAATCACACCTGCC‑G‑ATTTAGAACAAAACCAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGCCAG‑CCAGGGGCGTCCTTCCATTGATTTACTGCGCGC‑GTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGA  >  NC_000913/3293478‑3293916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑