Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,403,737 T→C 8.3% L85P (CTC→CCC)  acuI → putative acryloyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,403,7370TC 8.3% 200.0 / 2.6 70L85P (CTC→CCC) acuIputative acryloyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base C (3/4);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CAGGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTG  >  NC_000913/3403508‑3403975
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
cagGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCCctggctg                                                                                                                                                                                                                                       >  1:501199/1‑239 (MQ=255)
          aaaCTCTCCCCTCAGTACCGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATTTCACGGTCGATGTTCACTGCGCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCCTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGt                                                                                                                                                                                                                             <  1:906875/239‑1 (MQ=255)
             cgctcGCATCCGTACAGACTCTGGGCGAAAGTCGCCTGCCGGATGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa                                                                                                                                                                                                                          <  1:283272/237‑1 (MQ=255)
             cctccgtaTCAGTACAGGCTTGGGACGAAATTCGCCTGCTGGAGGGAGATGGCACGGTCGATGTTCAATGGTCGAGCCTGAACTATAAAGATGCGCTGGTGATTCCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa                                                                                                                                                                                                                          <  2:448002/232‑1 (MQ=255)
                  gCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAAcc                                                                                                                                                                                                                      >  1:853841/1‑238 (MQ=255)
                  ccctccgtccAGACTTCGGAAGAAAGGCGCCTGCCGGGGGGCGATGTCACGGTCTATGTTGACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa                                                                                                                                                                                                                     <  2:772423/230‑1 (MQ=255)
                         tACAAACTCTGGAAGGAAGTCCGCTGCCGGGGGGCGATGGCACGGTCGATGTTCACGGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTGCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCGCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTgggg                                                                                                                                                                                                               <  2:501199/238‑1 (MQ=255)
                            cgACTTTGGAAGAAAGTCGCCTGCGGGGGGGGGCTGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAACCATCCGTAATTTGCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTggg                                                                                                                                                                                                           <  2:369426/238‑1 (MQ=255)
                              aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTCCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATTCCGGTCAGGAGGTGTTACTCACTGCCTGGGGGGTTGGGGAAAACCACTGGGGTgg                                                                                                                                                                                                            >  1:122924/1‑236 (MQ=255)
                              aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGCACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCCGGCGGCGTTACTCACTGGCTGGGGCGTTGGTGAAAACCAATTGGGTgg                                                                                                                                                                                                            >  1:886016/1‑236 (MQ=255)
                              aCTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTgg                                                                                                                                                                                                            >  1:750979/1‑236 (MQ=255)
                               ccttGGACGAAAGTGGCCTGCCGGAGGGCGATGGCACGGTCGATTTTCACTGGTCGGGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAAGTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTggg                                                                                                                                                                                                           <  2:853841/233‑1 (MQ=255)
                                tctGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGaa                                                                                                                                                                                                                          <  1:846817/220‑1 (MQ=255)
                                  tGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACCCACTGGCTGGGGCGTTGGTGAAAACCCCTGGGGGGGGCggggg                                                                                                                                                                                                     >  2:625968/1‑237 (MQ=255)
                                  cggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGGAAACCACTGGGGGGGGCTGGCg                                                                                                                                                                                                     >  1:903197/2‑239 (MQ=255)
                                   ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCGGGTGGTGTTACCCACGGGATGGGGCGGTGTGGAAAACCACTGGGGGGGGATGGCgg                                                                                                                                                                                                    >  1:379046/1‑239 (MQ=255)
                                   ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGGGGGGTTACTCACTGGCTGGGGCGTTGGGGAAAACCACGggggggggggggggg                                                                                                                                                                                                    >  2:718957/1‑232 (MQ=255)
                                   ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTc                                                                                                                                                                                                                                               <  2:94842/196‑1 (MQ=255)
                                   ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTc                                                                                                                                                                                                                                               >  1:94842/1‑196 (MQ=255)
                                   ggACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGGGTTACTCCCTGGCTGGGGGGGTGGGGAAAACCCCTGGGGGGGGGcggcgg                                                                                                                                                                                                    >  2:549312/1‑239 (MQ=255)
                                          aGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCCGGAGGTGTTACTCACTGACTGGGGCGTTGGCGAAAACCACTGGGGTGGGCTGCCCGAGCCGgc                                                                                                                                                                                             >  1:84147/1‑239 (MQ=255)
                                          aGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCAGGGGCGTTGTTGACAAc                                                                                                                                                                                                                       >  1:238634/1‑213 (MQ=255)
                                                    cGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGaa                                                                                                                                                                                    <  1:56617/238‑1 (MQ=255)
                                                             aTGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGACCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGCGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGActgg                                                                                                                                                                          >  1:206704/1‑239 (MQ=255)
                                                                         aTGTTCACTGGCCGAGCCTTAACTATAAAGATGCGCTGGCGATTGCCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCAt                                                                                                                                                               <  1:411532/238‑1 (MQ=255)
                                                                              cACGGGGCGAGCCTGGACTATAAAGGGTACGTGGCGAGTCCCGGTAGGGGAAAAATCATCCGTAATTTTCCGATGATTCCTTGGATCGATGTTGGCGGAACTGTACGCTCCAGCGAAGATCCGCGTTTTCATGCCGGTCGGGAGGGGTTACTCACTGGCTGGGGCGTTGGGGAAACCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                                                                         <  2:404622/239‑1 (MQ=255)
                                                                                    tCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa                                                                                                                                                                                                                     <  1:622296/173‑1 (MQ=255)
                                                                                    tCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCa                                                                                                                                                                                                                     >  2:622296/1‑173 (MQ=255)
                                                                                    tCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGGGACGGCGGGGGGGGAAGGGGACGGGCTGGGTGCCATCCCCCAAggggg                                                                                                                                                   >  2:176759/1‑237 (MQ=255)
                                                                                        cgcTGAACTTAAAAGGTGCGCTGGCAATTACCGGTAAGGGAAAAATCCTCCGTAATTTTCCGATGATTCCTGGGATCGATTGTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                                                                               <  1:23333/237‑1 (MQ=255)
                                                                                                 ttaAAGATGCGCTTGGGATTCCCGGTAAGGGAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg                                                                                                                                     <  1:710953/238‑1 (MQ=255)
                                                                                                  taAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCActggctg                                                                                                                                                                                                                                       >  2:439038/1‑141 (MQ=255)
                                                                                                  taAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCActggctg                                                                                                                                                                                                                                       <  1:439038/141‑1 (MQ=255)
                                                                                                  taAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGGGAGTGAAAGGTGACTGGCCGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg                                                                                                                                     >  1:400709/1‑239 (MQ=255)
                                                                                                  taAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAg                                                                                                                                     <  1:608410/239‑1 (MQ=255)
                                                                                                        aTGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGGGAAAGGTGACTGGCTGGTTGACATGCCGCAAGGGCTGGACGAGGGCAAAGCAATg                                                                                                                                >  1:139755/1‑239 (MQ=255)
                                                                                                           cgcTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt                                                                                                                             >  1:654604/1‑239 (MQ=255)
                                                                                                           cgcTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGGGAAAGGGGACTGGCTGGTTGCCCTGCCGCAAGGGCTGGAGCCGCGGAAAGCAAGGAtt                                                                                                                             >  1:47753/1‑239 (MQ=255)
                                                                                                              tGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTAt                                                                                                                           <  1:549312/238‑1 (MQ=255)
                                                                                                                 gggttACCGGTAAGGGAAAAATTCTCCGTAATTTTCCGTTTATTCCTGGGATCGATTTTGCCGGAACGTTACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg                                                                                                                        <  2:287868/235‑1 (MQ=255)
                                                                                                                 ctaTTACCGGTAAGGGAAAAATCATCCGTAAGTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGAACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg                                                                                                                        <  1:176759/236‑1 (MQ=255)
                                                                                                                 ccaTTACCGGTAAGGGAAAAATCATCCGTAATTTCCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg                                                                                                                        <  1:291490/236‑1 (MQ=255)
                                                                                                                       ccGGAAAGGGAAAAATCCCCCGCTATTTCCCGATTATTCCTGGGGTCGTTTTGGCCGGACCTGTACGCCCCGGCGAAGATCCGCGTTTTCATGCCGGTCGGGAGGTTTTACTCACTGGCTGGGGCGTTGTTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc                                                                                                                 <  2:654604/239‑1 (MQ=255)
                                                                                                                          gTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAAGCGGGTAAGGCAATGATAATCGGTAATGCCGGt                                                                                                              >  1:681441/1‑239 (MQ=255)
                                                                                                                                gAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                                          <  1:625968/237‑1 (MQ=255)
                                                                                                                                aaaaaaTCATCCGTAATTTTCCGATGATTCCTGGTATCGATTTTGCCGGAACTGTTCGGACCAGCGAAGATCCGCGTTTTCAGGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAACCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                                          <  1:915018/236‑1 (MQ=255)
                                                                                                                                   aaaTCCTCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGTTCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc                                                                                                     <  1:718957/239‑1 (MQ=255)
                                                                                                                                   aaaTCATCCTTATTTTTCCGTTTTTTCCTGGGTTCGTTTTTGCCGAGACGGTCCGCCCCGGGGAAGTTCCGGGTTTTCATGCCGGTCAGGAGTTGTTACTCCCTGGTGGGGGCGTTGGTGAAAACCCTTGGGGGGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCAGGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc                                                                                                     <  2:47753/239‑1 (MQ=255)
                                                                                                                                    aaTCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                                                                         <  2:786731/186‑1 (MQ=255)
                                                                                                                                    aaTCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                                                                         >  1:786731/1‑186 (MQ=255)
                                                                                                                                         tcCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGGGGGAAAGGGAACCGGCGGGTTGCCATGCCCCCAGGGGTGGGCCCGCGCAAAAGAATGATTTTTGGTCATGCCGGGTTTTACGCCCTgggg                                                                                               >  2:896904/1‑236 (MQ=255)
                                                                                                                                                            tCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCCTGCTGTTCGGGATGGCGCTGGAAg                                                                            >  1:15064/1‑239 (MQ=255)
                                                                                                                                                                              ggggagtGTCCGCACAGGGGAATATCCGGGTTTTCAGGCCGGTTGGGGGGTGTTACCCCCGGGGTGGGGCGTGGGTGAAACCCCCGGGGGTGGGCTGGCGGGGCAGGCGCGGGTGAAAGGTGCCGGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                                                           <  2:522080/232‑1 (MQ=255)
                                                                                                                                                                              cGGAACTGTCCGCCCCAGGGAAGTTCCGCTTTTTCATGCCGGTCAGGAGGTGTTACTCGCTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGc                                                          <  1:135795/239‑1 (MQ=255)
                                                                                                                                                                                      tACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGGGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACggg                                                  >  1:522080/1‑239 (MQ=255)
                                                                                                                                                                                       aCGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAGACCACTGGGGTGGGCTgg                                                                                                                                                                                                       >  2:351026/1‑89 (MQ=255)
                                                                                                                                                                                       aCGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACCCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTgg                                                                                                                                                                                                       <  1:351026/89‑1 (MQ=255)
                                                                                                                                                                                           ccaagggAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTCCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGgaga                                              <  1:531293/232‑1 (MQ=255)
                                                                                                                                                                                                     ttCCGGGTTTGCAGGCCGGTCAGGGGGTTTTACCCCCTGGCGGGGGGGTTGGGGAAACCCGATGGGGTGGGCTGGCGGAGCAGGCGCGAGTTAAAGGTGCCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACg                                   <  1:135527/238‑1 (MQ=255)
                                                                                                                                                                                                          cgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGGGATTGTCGTGACGGGGGc                              >  1:395625/1‑239 (MQ=255)
                                                                                                                                                                                                                                tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTgtgg                                                                                                                                                                                                               <  1:847321/40‑1 (MQ=255)
                                                                                                                                                                                                                                tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                                          <  1:705221/141‑1 (MQ=255)
                                                                                                                                                                                                                                 gtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAGGGGGAGATTGTCGTGACGGGTGCCAGGGGTGGCGTCGGCCGTAcc        >  1:882867/1‑238 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                                                                               >  1:452368/1‑102 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                                                                               <  2:452368/102‑1 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt                                                                                                                             >  1:656077/1‑120 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGAtt                                                                                                                             <  2:656077/120‑1 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc                                                                                                                 <  1:848035/132‑1 (MQ=255)
                                                                                                                                                                                                                                  ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGAAATGGTTATCGGTACTGcc                                                                                                                 >  2:848035/1‑132 (MQ=255)
                                                                                                                                                                                                                                    aCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCCGGACGGGTGCCCGGGGGGGGGTCGTCAGGAccggcg    >  1:687793/1‑239 (MQ=255)
                                                                                                                                                                                                                                      tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGGGATTGTCGTGACGGGTGCCAGTGGGGGCGTCGGCAGTACCGCCGTg  >  1:909480/1‑239 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
CAGGACGGCAAAACTCTCGCATCAGTACAGACTCTGGACGAAAGTCGCCTGCCGGAGGGCGATGTCACGGTCGATGTTCACTGGTCGAGCCTGAACTATAAAGATGCGCTGGCGATTACCGGTAAGGGAAAAATCATCCGTAATTTTCCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTCGGCAGTACCGCCGTG  >  NC_000913/3403508‑3403975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: