Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,489,862 A→G 21.7% S107P (TCC→CCC)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,489,8620AG21.7% 129.4 / 3.6 61S107P (TCC→CCC) argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (13/32);  new base G (13/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.51e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCATCCATCACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCT  >  NC_000913/3489625‑3490086
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      
gATCATCCATCACCGCTTTCCATGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAg                                                                                                                                                                                                                                       <  1:515716/239‑1 (MQ=255)
    atccatcACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCCTTTGCTTTCCGGCCGGAGtcat                                                                                                                                                                                                                                   >  1:278595/1‑236 (MQ=255)
      ccatcACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGGTTTGTTAGCTTCCGTGCCGGAGTTCCTa                                                                                                                                                                                                                                >  1:498884/1‑239 (MQ=255)
      ccatcACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGGTTCGTTAGCTTCCGTGCCGGGGGTCATa                                                                                                                                                                                                                                >  1:45986/1‑239 (MQ=255)
       catcACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGTCCACCCACCGAACCGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCATGCCAGTTTAAAGGCGGTTTCGTTAGGTTCCCGGCCGGaaggtagaa                                                                                                                                                                                                                                >  2:622752/1‑231 (MQ=255)
       catcACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGATTCCCGGCCGGAGTGCCTaa                                                                                                                                                                                                                               >  1:360073/1‑239 (MQ=255)
        tcccccccTTTCCCTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATaaa                                                                                                                                                                                                                              <  2:328033/232‑1 (MQ=255)
           aCCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTTGTTTTGAACGGGCTATGACGACCACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTGCGTAGATTCCATCCCCGGGTTCCATAAac                                                                                                                                                                                                                             >  1:916056/1‑238 (MQ=255)
               cTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGGGCCGGGGTTCTTAAAcaccac                                                                                                                                                                                                                      >  1:132972/1‑238 (MQ=255)
                       gCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGACCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCAACCAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAACTTCCGGTGCCGGGGTCAATAAACCCCCcggttacgg                                                                                                                                                                                                              >  1:880530/1‑232 (MQ=255)
                        cATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTCAGTTTCCGTGCCCGAGTTCATAAaaccacaccgttctgac                                                                                                                                                                                                           >  1:118516/1‑228 (MQ=255)
                             agaTCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTTGTTAGCTTTCCTGCCGGGGTTCATAAACACCCCCCGTTCGGCAAAcct                                                                                                                                                                                                       >  1:638912/1‑237 (MQ=255)
                              gaTTGTTAAAGGTGACGTGGATGATGTGTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTGGGCTGCCCACCCCACGAAACGGTAAACCAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTGTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACg                                                                                                                                                                                                        <  1:251518/238‑1 (MQ=255)
                                  gTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTTGGTAACTTCCGTGCCGGGGTTCCTAAAAACCCACCGTTCGGCAAACGTTgtc                                                                                                                                                                                                   >  1:283433/1‑236 (MQ=255)
                                  gTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTTGCTTCCCGTCCCGGGTTCCTAAAAAACACCCGTTCGGCAAACGGTGcc                                                                                                                                                                                                   >  1:785043/1‑238 (MQ=255)
                                      aaGGGAACGGGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAAt                                                                                                                                                                                             <  1:622752/239‑1 (MQ=255)
                                           aaCGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCCTAAAAACCACCCGTTCGGCAAACCTTGCCTCAAtacgtt                                                                                                                                                                                        >  1:544857/1‑234 (MQ=255)
                                           aaCGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTGTCGCTTTCTTTCGGGCCCGGGTTTATAAACACACACCGTTTGGCAAACGTTTcccccaacaagt                                                                                                                                                                                         >  2:702750/1‑229 (MQ=255)
                                           aaCGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGGTTTGGTAGATTTCCGGCCGGGGTTCATAAAACCCACTGGGTCGGCAAACGTTGCCTCCATAAGtt                                                                                                                                                                                        >  1:616463/1‑239 (MQ=255)
                                           aaCGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGGTTCGTTAGCTTCCGTGCCGGAGGTCATAAACACCACGCGGTCGGCAAACGTTGTCTCAATCAggt                                                                                                                                                                                        >  1:543868/1‑237 (MQ=255)
                                           aaCGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGCTTCTTTTGCCTCCGGTCCCGGGGTCATAACAACAACCCGTGTGGCCAACGGTGACTCaaacc                                                                                                                                                                                           >  2:126879/1‑233 (MQ=255)
                                             cGTTGATGATGTCTGCCGGTTGCGGCCCAAAGCCGTCGGAATATTGTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTa                                                                                                                                                                                      <  1:265070/239‑1 (MQ=255)
                                             cGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCCAACAGGCGTAATGGCGTGCCAGTTTAAAAGCGGGTTTGCTTGCTTCCCGTCCCGGGGTTAAAAAAACCACGCGGTCGTCCAACGTTTGCTCACACCAGttt                                                                                                                                                                                       >  1:882237/1‑238 (MQ=255)
                                              gTGGGTGATGTGTGCCGTTTTCGGCCCAAAGCCGGTGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTAc                                                                                                                                                                                     <  2:482279/239‑1 (MQ=255)
                                                     atgTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTTCGTGCCGGGGTTCATAAACACAACGCGTTCGGCAAACGTTGCCTCCATCCGTTTTCGccccaa                                                                                                                                                                              >  1:131724/1‑236 (MQ=255)
                                                            ccGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCTGGCCGGAGTTCATAAACACCACGCGTTTGGCAAACGTTGCCCCCACCAGTTTACGCCCCACACGCac                                                                                                                                                                        >  1:881255/1‑237 (MQ=255)
                                                                       ccAACGCCGTCGGATTATTTTGGCGTCCCACCCGCCGAAAGGGTAAAAAGCGAGCGAACATGAAAAGCGTTATGGAAGGCAATAAGTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCAtt                                                                                                                                                            <  2:715732/239‑1 (MQ=255)
                                                                       ccAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCAt                                                                                                                                                             <  1:48944/238‑1 (MQ=255)
                                                                          aaGCCGTGGGAATATTTTGGGTGCCCACCCCCCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGt                                                                                                                                                         <  2:881255/239‑1 (MQ=255)
                                                                            cccGGCGGAATTTTTTGGCCGCCCCCCCACCGAAACGGTAAACAGGGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGa                                                                                                                                                       <  2:132972/238‑1 (MQ=255)
                                                                              cggaggAATATGTTGGCTGCCCCCCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGa                                                                                                                                                       <  1:126879/233‑1 (MQ=255)
                                                                              cGTCGGAATTTTTTGGCCGCCCACCCAACGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGaa                                                                                                                                                      <  2:283433/238‑1 (MQ=255)
                                                                              cGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGaaa                                                                                                                                                     <  1:143149/239‑1 (MQ=255)
                                                                               ggcGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGaaaa                                                                                                                                                    <  1:279403/237‑1 (MQ=255)
                                                                               gTCGGAATTTTTTGGCTGCCCACCCCCCGAAACGGTAAAAAGCGAGCGACCATGAAAAGGTTTAAGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGaa                                                                                                                                                      <  2:785043/237‑1 (MQ=255)
                                                                                       tttttGGCCGCCCCCCCACCGAAACGGTAAACAGCGAGCGACCAGGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTg                                                                                                                                            <  2:544857/238‑1 (MQ=255)
                                                                                          ttGGCCGCCCCCCCACCGAAACGGTAAACAGCGGGCGACCATGAAAGGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGata                                                                                                                                         <  2:278595/239‑1 (MQ=255)
                                                                                                 cccacccaCCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCcaca                                                                                                                                  <  1:823203/239‑1 (MQ=255)
                                                                                                  ccccccaacGAAACGGTAAGAAGCGATGGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCcaca                                                                                                                                  <  1:369613/235‑1 (MQ=255)
                                                                                                            aaaCGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGccc                                                                                                                       <  2:716294/239‑1 (MQ=255)
                                                                                                                ggTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGGTCGGCAAACGTTGCCTCAATCAGGTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGGTATGCCCCAGAGTTTCGCCCCggg                                                                                                                  >  1:223783/1‑239 (MQ=255)
                                                                                                                           gagcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGGTTACGCCCAAGACGCAGCGCCGGGTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGGTTTTTaagc                                                                                                         >  1:80305/1‑237 (MQ=255)
                                                                                                                             gcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAAcgcg                                                                                                       <  1:702750/238‑1 (MQ=255)
                                                                                                                                    tGAAAAGCGTTATGGAAGGCAATAATTCTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGaa                                                                                                                                                      <  1:503054/185‑1 (MQ=255)
                                                                                                                                           ggTTAGGGAAGGCAATAATTTTGGTTTTGAACGGGCTATTACGCCCACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGa                                                                                        <  1:284918/238‑1 (MQ=255)
                                                                                                                                             ttttGGAAGGCAATAATTTTGGGTTTGAACGGGTTTTGACGCACCCAGGCGTAAGGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGAt                                                                                       <  2:638912/235‑1 (MQ=255)
                                                                                                                                                         attatTTTGGTTTTGAACGGGTTTGGGCCCACAAGGGCGTAATGGCGTGCCAGTTTAAAGGGGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACCCCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGCTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCaa                                                                          <  2:360073/236‑1 (MQ=255)
                                                                                                                                                          tgatttgtgTTTTGAACGGTCTATGACGCACCCGGGCGTACGGGGCTGCCAGTTTAAGGTCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACCCCGCGCGTTCGGCAAACGTTGCCTCAATCAGTTCCCGCCCAAGACGCCTCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCAAAc                                                                         <  2:157509/231‑1 (MQ=255)
                                                                                                                                                                                     gCACCCAGGCGTATTGGGGTGACAGTTTAAAGGGTGTTTACTTAGCTTCCGGGCCGGGGTTCATAAACCCCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGaaa                                              <  2:45986/239‑1 (MQ=255)
                                                                                                                                                                                      cacacaGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAAt                                             <  1:131272/239‑1 (MQ=255)
                                                                                                                                                                                       acacaGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATa                                                                                                                                                                                                                                <  2:442508/63‑1 (MQ=255)
                                                                                                                                                                                       acacaGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATa                                                                                                                                                                                                                                >  1:442508/1‑63 (MQ=255)
                                                                                                                                                                                       acacaGGCGTAATGGCGTGCCAGTTTAAAGGCGGGTTCGTTAGCTTCCGTGCCCGGGTTCATAAACAACACCCGGTCCGCAAACCTTGCCTCAATCAGTTTACGCCCCAGACGCAGCCACGGGTCATTGGGGAAAACGTTACTAAAATGCCACAAGGATTCGCCCTGGGTTTTTAAAGCGTTCACACCCCCCGGAACGGAAAGGCCCCACCCCGTACATCCAATGCCAccccccgaaac                                            >  2:518889/1‑231 (MQ=255)
                                                                                                                                                                                           aGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGGGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGGTTACGCCCAAGACCCCGCGCCGGGTCATTGGTGAAAACGTTACTGATATTCCACAGAGGTTCGCCCTGGGGTTTTAAAGCGTTCACCCACCCCGGGTGGCAAAGGCCCCACCCCCTAAATACAATGACACCCCCGCAAACGACa                                        >  2:118276/1‑239 (MQ=255)
                                                                                                                                                                                                tAATGGCGTGCCAGTTTAAAGGCGTTTTCCTTAGGTTCCGTGCCGGAGTTCATAAACACCACGGGTTCGGCAAACGTTGCCTCAATCAGGTTACGCCCAAGCCGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACt                                    <  1:652886/238‑1 (MQ=255)
                                                                                                                                                                                                       gTGCCAGTTTAAAGGGGGTTTCGTTTGCTTCCGTGCCGGAGTTCATAAACACCCCGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTccttgcc                            <  2:498884/238‑1 (MQ=255)
                                                                                                                                                                                                         gCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGGTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATTCCACCCCCCGAATCGACAAAATccttgcctt                          >  1:715000/1‑238 (MQ=255)
                                                                                                                                                                                                                        gTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGGTTACGCCCAAGACACAGCCCCGGTTCATTGGGGGAAACGTTACTGTTATGCCACAGAGTTTCGCCCTGGGGTTTTAACTCGATCACCCGCGCCGAGTGGGAATGGGCCCACGGCGTAACTGCAAAGCCACCCCCGCAATCGACAAACCCCTTTCCCTTCCTGACCCCacgta            >  2:833067/1‑234 (MQ=255)
                                                                                                                                                                                                                                tgtctCCGTGCCGGGTTTCAAAACACCCAACCGGTCGGGAAAGGTGTCCTCAATCAGTTTACGCGCAAGTAGCAGGTCCGTTTCAATGGTGAAAACGTTACTGATATGCCACAGAGGTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCt  <  2:916056/235‑1 (MQ=255)
                                                                                                                                                                                                                                aGCTTCCGTGCCGGAGTTCATAAACCCCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCt  <  1:138094/239‑1 (MQ=255)
                                                                                                                                                                                                                                   ttCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCAc                                                                                                  <  2:16028/141‑1 (MQ=255)
                                                                                                                                                                                                                                   ttCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCAc                                                                                                  >  1:16028/1‑141 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      
GATCATCCATCACCGCTTTCACTGCATGGAGATCGTTAAAGGGAACGTGGATGATGTCTGCCGGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCT  >  NC_000913/3489625‑3490086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: