Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,587,589 | A→G | 14.0% | E214G (GAG→GGG) | mrr → | methylated adenine and cytosine restriction protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,587,589 | 0 | A | G | 14.0% | 185.9 / 3.1 | 71 | E214G (GAG→GGG) | mrr | methylated adenine and cytosine restriction protein |
Reads supporting (aligned to +/- strand): ref base A (23/37); new base G (9/2); total (32/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.77e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
TGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGTTTCTTCACGTTTGTT > NC_000913/4587353‑4587826 | tGCAAAGAGCAGCCCGGGCGATCGGTTAAATCAGGCGGTAAAAGAGCTTCGTGATGCAGTGGCTGATGAGGTTCTGTAAAACTTATTGCATGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGgaga < 1:462349/239‑1 (MQ=255) tGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTTGCGGGACTTGAGATGGGGGGATCGCTGGTGTGGTATCCCTTTGTAAACTTGGCCTGggga > 1:158948/1‑239 (MQ=255) cccGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGGGATGGTTTTCAGCGTGTTGGGGGGACTGGGGATGGGGGGATCCCTGGGGTGATAACGCTGGTAAAAAAAGGCCTGGGGAAAAATTATGttt > 2:319648/1‑238 (MQ=255) ctggACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGtt < 1:560156/235‑1 (MQ=255) tCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTTCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGGTAAAATTGGCCTGGAGAAAGGTTATGTTTAGGCAAAAc > 1:128861/1‑239 (MQ=255) cGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGGGATGGTGGCATCGGTGGGGTGGTATCGCTTGGTAAACTTGGCCTGGGGAAAGTTTTTGTTGTGGGAAAACg > 1:393618/1‑239 (MQ=255) ggTTTGAGCTGGCGCTAAAAGAGCTTCGTGACGCGGTGGCTGAGGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGGTTTGAAGGCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGt < 2:421022/239‑1 (MQ=255) ggTTAGATCAGGCGCTAAAAGGGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAATCTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGt < 1:913755/239‑1 (MQ=255) gttaagtCAGGCGCTAAATGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGGCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCGACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTTAGGCAAAACGt < 1:227767/232‑1 (MQ=255) gATCAGGCGCTAAAAGTGCTTCGTGATGCGGTGGTTTAGTGGGTTCTGGAAATCTTATTGCAGGGTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCa < 1:435386/239‑1 (MQ=255) gtaGGGGCTAAAAGAGGTTCGGGTTTCGGTGGCTGGTGAGGTTCTGGAAAACTTATGGCAGGTTTCTGCTTGGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGGGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGa < 2:667103/237‑1 (MQ=255) cAGGCGCTAAAAGGTGTTCGTTATGCGGTGGCTGATGAGGTTCTGGAAAACTTAGTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGa < 1:797719/238‑1 (MQ=255) cAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCACCGCCTGGGGAATGGCGGCCACCGTGTGGATTTGCAGGCTCTTGGCGGTACTGGGGACGGGGGCATGAAGGGGGGGATCTGGCGTGATAAACTTGCACTGGAGGAAGTGTTGGTTGGGGCAAAAAATTGGCGGaa > 2:762077/1‑239 (MQ=255) aaaaGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTTGAGATGGTGGCATCGATGGGGTGATATCGCTTGTTAAACTTGGCCTGGGGAAAGTTTTTGTTCAGGGAAAACTGTGGCAGAATACTGTag > 1:225937/1‑239 (MQ=255) aaaGAGCTTCGTGATGCGGTGGCTGATGAGGTTCAGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGGATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTagg < 1:103904/239‑1 (MQ=255) tCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGGGATGATTTGCAGCGTGTTGGGGGTACTGGGGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTTGCCTGGAGAAAGTTTATGGTCAGGCAAAACGTTGGCGGAATACTGTAGGCAGGCCAg > 1:182732/1‑239 (MQ=255) tggggtgggtgGTGGGGTTTTGGAAAATTTTTTGCAGGTTTCTTCTTCGGGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑c < 2:771922/236‑1 (MQ=255) tGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGGGGCATCGATGGGGTGATATCGCTTGGTAAACTTGGCCTGGGGAAAGGTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCa > 1:82110/1‑239 (MQ=255) gCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTTTTTGCGGTACTGGAGATGGTGGACTCGCAGGTGGGGTTTCCCTTGTTTAAATTGTGCTTGGGGAAGATTAGTTTTCGGCAAAAAGTGGGACGAAAAAGTTAGGGGGGCCAGAAATA‑AAAGCAtt > 2:685232/1‑239 (MQ=255) tggggTTCTGGAAAACTTATTGGAGGTTTCCCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACg < 1:319648/236‑1 (MQ=255) tgaGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTAc < 1:583342/238‑1 (MQ=255) cTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTTCAGCGTGTTGGCGGTACTGGGGATGGTGGCATCGATGGTGTGATATCGCTTGATAAAATTGGCCTGGGGAAAGTTTATGTTCAGGCAAAAAGTTGGCCGAATACTGTAGGCAGGCCCGAATTA‑CAGGCATTTTACGGGGCC‑CCGGCTGGGc > 1:152139/1‑239 (MQ=255) gTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGGTATCGCCTGATAAACTTTGCCTGGGGAAAGGTTATGTTCCGGCACAACGTTTGCAGAATACTGTAGGCAGGCCCAAATTA‑CAGGCAATTTAAGGCGCC‑CTGGCTTGGGAAAAA‑AC‑Gaa > 1:892692/1‑239 (MQ=255) ttttcccTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑Gaa < 1:519468/233‑1 (MQ=255) cccTTCGGGTTTTGTAGTCTTTTTTTTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGTGATGATTTGCAGCGTGGTGGCGGTTCTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTg < 2:393618/238‑1 (MQ=255) cgcgCTTTGAAGTCCTTGTTCTGGATGTTTTGCATCGGCTGGGGTAAGGAGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCCTCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGgtg < 1:717542/239‑1 (MQ=255) gAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTAc < 1:59638/239‑1 (MQ=255) ggATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTTAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACCGCGCA‑CCTGCTGGGGAAAAAGGC‑GAAA‑GTGGGGTGGTTAATACCAATTTCGGGTTTa > 1:714162/1‑239 (MQ=255) gATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGGGATGGTGGCATCGATGGGGGGATATCGCTTGGAAAACTTGGCCTGGGGAAAGTTTTTGTTTATGCAAAACGTTGGCAGAAAACTGTATGAAGGCCAGAATTT‑AAGGGAATTTTATGCGCACCTGTCGGGCAAAAAA‑GG‑GAAACAGGGGGGGTTATTTACCCCTCTTGGTTTTa > 2:777436/1‑239 (MQ=255) tttGCATCGCCTGGGGTATGGCGGCCACCGTGGTGATTTGCAGCGTGTTGGCGGTACTGGGGGTGGTGGCATCGCTGGGGGGGTATCTCTTGTGAAAATTTGCCTGGGGAAAGGTTTTGTTCCGGGAAAACTGTGGCCGCATACTGTAGGCCGGACAGCATTACCAGGC‑TTTTACGGCGGC‑CGACACGGGCAAAAA‑GCAGAAAAGGGGGGGGTTTATAACCACTTTTgttttattttta > 2:470632/1‑228 (MQ=255) ggggTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGTCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACt < 1:794784/239‑1 (MQ=255) ggcggcCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGGGTGGTATCGCTTGATAAAATTTGCCTGGAGAAAGTTTATGTTTAGGCAAAACGTTGGCAGAAAACTGTAGGCCGGCCAGACTTA‑CAGGCATTTTTCGGCGCA‑CTTGCCGGGGAAAAA‑GC‑GGAACATGGGGGGTTTTTTACCCCTTTTGGATTTACTTTTCAGGCGCGGGACCTTGCCCa > 2:783477/1‑239 (MQ=255) cggcCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAGGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCaa < 1:648243/238‑1 (MQ=255) cACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGt < 1:470632/239‑1 (MQ=255) aCCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATAGCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGt < 1:184541/238‑1 (MQ=255) cGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGa < 1:186069/239‑1 (MQ=255) gttttGCCGCGTGGTGGGGGTTCTGGAGAGGGTGGCATCGGTTGGGTGATAACGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGTA‑CTGGCTGGTCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTa < 1:507668/236‑1 (MQ=255) tGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGgtg < 1:783477/239‑1 (MQ=255) tGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGGGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGCCGAGGGTATgggg > 1:354448/1‑237 (MQ=255) cAGCGTGTTGGCGGTCCTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGCATACTGTAGGCAGGCCAGATTTA‑CAGGCATTTTACGGCGCC‑CTGGCTGGGCAAAAA‑AC‑GAAACGTGGGGGGGTTATTACCACTCTTGGATTTAATTCTCAGGCGCGGGACTTTTCCCACACCGCCGCGGGTATGgttgt > 2:836302/1‑236 (MQ=255) gttttGGGGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTgttggt < 2:619747/235‑1 (MQ=255) tgtTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGGGTTTATTACCACTTCTGGGTTTACTTTTCAGGCGCGTGCCTTTGCCCAAACCGCCGAGGGTGTGGGgtgggttg > 2:552278/1‑239 (MQ=255) gtTGGCGGTACTGGAGAGTGTGGCATCGATGGTGTGATAGCGCTTGAGTAACTTGGCCGGGAGAAAGGTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGa < 1:739068/239‑1 (MQ=255) gttgCGGTACGGGTGTTGGGGGCATCGATGGTGTGATTTCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAGAACGTTGGCAGAATTCTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTTGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGAt < 2:182732/236‑1 (MQ=255) ggTATTGGGGATGGGGGGATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATgggg < 2:152139/238‑1 (MQ=255) ggTACGGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTTGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATgggg < 1:762077/238‑1 (MQ=255) gttcTGGAGATGGGGGAATCGATTGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGa < 2:128861/235‑1 (MQ=255) aagggAGATGGTCGCATCGATGGTTCGATATCGTTTGATAAACTTGGCCTGGGGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCGCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAAc < 1:735418/235‑1 (MQ=255) cTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTggg < 1:301937/152‑1 (MQ=255) cTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTggg > 2:301937/1‑152 (MQ=255) agaTGGTGGCATTGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCg < 2:56524/206‑1 (MQ=255) agaTGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCg > 1:56524/1‑206 (MQ=255) tggtggGATCGATGGTGTGATATCGGTTGAAAAACTTGGCCTGGAGAAAGTTTAAGTTCAGGCAAAACGGTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTg < 2:212083/238‑1 (MQ=255) tggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTg < 1:552278/238‑1 (MQ=255) tggtggCATCGATGGTGTGATATCCCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTg < 1:304931/238‑1 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTc > 2:744421/1‑201 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTc < 1:744421/201‑1 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGGGACTTTGCCCAATCCGTCGCGGGAATGGTGTTGGTTGATGGGGAACGCCTGGGGCa > 1:198335/1‑239 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGGGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGc > 1:417280/1‑238 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGAGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGGGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAAGGCCGGGTg > 1:450720/1‑237 (MQ=255) ggtggCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGCGAGGGGTTTATTACCCCTTCTGGATTTCCTTCTCAGGCGCGTGACTTTGCCCAATCCGCCGAGGGTATGGGGTTGGTTGATGGGGGACGCCGGGGGc > 2:703988/1‑238 (MQ=255) ttggATATCGCTTGATAAACTTGGCCGGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTaa < 1:843142/227‑1 (MQ=255) tgtgATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGCATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGaaa > 1:862724/1‑239 (MQ=255) cTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGGAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCAGTTTACGGCCCC‑CTGGCTGGGCAAAAA‑GC‑GAACCGTGGGGTGTTTACTACCACTTCTGGATTTACTTCTCAGGAGCGTCACTTTGCCCCATCCGTCGGGGGTATGGCGTTTGTGACAGGGGAAACCCTGGTGCCCCTACAGAACCAAAACGAaggagg > 2:37168/1‑239 (MQ=255) cTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCCTGCCAGAATTA‑CAGGCATTTTACGGCTCA‑CTGGCTGGGCAAAAA‑TC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTTCTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGGCGGTCTGTGTTTGGTTGGTGGGGCACGCCTGGTGCACTTAATGATAGAAAACGAAGTAgg > 2:776788/1‑239 (MQ=255) cTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGGGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAgg > 1:301741/1‑239 (MQ=255) aTAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTgggg < 2:606338/117‑1 (MQ=255) aTAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTgggg > 1:606338/1‑117 (MQ=255) aTAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGtt > 1:687926/1‑239 (MQ=255) tGGCCTGGGGAAATTTTTTTTTCAGGTAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGTTTCTTCAc < 2:354448/239‑1 (MQ=255) gCCGGGAGAAAGGTTAAGTTCGGGCAGAACGTTGGCAGAATCCTGTAGGCAGGCCAGGATTA‑CAGGCATTTTGCGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGGGGGGTGTTTATTACCTGTTCTGGATTTACTTCTCAGGTGCGTGACTTTGCGCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGTTTCTTCACGt < 1:37168/239‑1 (MQ=255) ccTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGTTTCTTCACGtt > 1:140634/1‑239 (MQ=255) ccTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGGTTTGGTTGATGGGGAACGCCTGGTGCCCTTAATGATCGAAAACGAAAAAGGGGTGTTTTCAAGtt > 2:710044/1‑239 (MQ=255) gagaAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATTGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGCAAACGAACTAGGGGTTTCTTCCCGTttgtt > 1:226147/1‑239 (MQ=255) | TGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCAAAACGTTGGCAGAATACTGTAGGCAGGCCAGAATTA‑CAGGCATTTTACGGCGCA‑CTGGCTGGGCAAAAA‑GC‑GAAACGTGGGGTGTTTATTACCACTTCTGGATTTACTTCTCAGGCGCGTGACTTTGCCCAATCCGTCGAGGGTATGGTGTTGGTTGATGGGGAACGCCTGGTGCACTTAATGATCGAAAACGAAGTAGGGGTTTCTTCACGTTTGTT > NC_000913/4587353‑4587826 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |