Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,395 T→C 8.2% intergenic (+135/+285) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,3950TC 8.2% 135.2 / 4.6 49intergenic (+135/+285)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (31/14);  new base C (2/2);  total (33/16)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

GCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACA  >  NC_000913/4614159‑4614605
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                    
ctcggggtgtTGCCCGTTTTTTGCGGGTTGCGGCGGGAACCGGGGGGTGGGATTGCCGAAGCGTGGGTTAAAACACCGGATGCGTGGTTTAACGGGCGGTGGGCCGGATAAGGGGTTCCCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                                                                                                                                                                                                                    <  2:145257/233‑1 (MQ=17)
                     tGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGCCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCCCAATGCCTGATGcacc                                                                                                                                                                                                >  1:784392/1‑235 (MQ=255)
                           ttggcccGGGACCCGGGGGGTGGGATTGGGCAAGGGTTTGTTAATAACCGGTATCCTGTGTTTAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCAAAAGGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTc                                                                                                                                                                                         <  2:877008/234‑1 (MQ=17)
                           ttGGGCCGGGAACCGGGGGATGGGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGt                                                                                                                                                                                          <  1:340360/238‑1 (MQ=255)
                            tGCGCCGGGAACCGGGGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATCCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTc                                                                                                                                                                                         <  1:845375/238‑1 (MQ=255)
                            tGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCttttgc                                                                                                                                                                                        >  1:89562/1‑235 (MQ=255)
                                      aCCGGCGGATGGAATGCCGTAAGCGGTGTTTAATACCACGTAAACGTTGTTTAACGTGCCGTAGGCTGGATAAGTCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCTTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATc                                                                                                                                                                               <  1:345057/238‑1 (MQ=17)
                                      aCCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGGTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATc                                                                                                                                                                               <  1:227303/238‑1 (MQ=255)
                                             ggtggtaTTGCCCAAGCGTTGCTTAAAAACACGTATACGTTGTTTAACGTGCGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTa                                                                                                                                                                        <  1:602708/232‑1 (MQ=255)
                                                     tGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGGTGCGACGCTTTTCGCGGCCTAACATGCCTCCAAGTCtgt                                                                                                                                                               >  1:679593/1‑239 (MQ=255)
                                                            gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCAACAAGTCCGTGCCGaa                                                                                                                                                         >  1:138776/1‑238 (MQ=255)
                                                                tGCTTAATAACAGGTTGACGTGGTTTAACGGGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGt                                                                                                                                                     <  1:676232/238‑1 (MQ=255)
                                                                   ttAAAACCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAgg                                                                                                                                                  <  1:578513/238‑1 (MQ=255)
                                                                             cGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGGCCCACAATGCTCGATGTGACGCTTGTCGCGTCTTATCATGCCTAGAAGTCTGTGCCGAACCGTAGGCCGGATacg                                                                                                                                         >  1:796369/1‑236 (MQ=255)
                                                                                ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGATTGCGCACAATGCCTGATGCGACGCTGGTCGCGTCTTATCTTGCCTACAAGCCTGTGCCGCACCGTAGGcccgtcccttat                                                                                                                                      >  1:859329/1‑228 (MQ=17)
                                                                                 taCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTTGGCCGAACCTTAGGCCGGTTAAGGCGt                                                                                                                                     >  1:486948/1‑238 (MQ=255)
                                                                                      tgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTTGGCCGAACCCTAGGCCGGATAAGGCGTTCAcg                                                                                                                               >  1:808966/1‑238 (MQ=255)
                                                                                      tgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGAGCACAATACCTGATGCGACGCTTGACGCGTCTAATCATGCCTACAAGTCTGTGCCGAACCGAAGGACGGCTAAGGAGTTCAcg                                                                                                                               >  1:914112/1‑238 (MQ=14)
                                                                                      tgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACACGTTTTTACCGACCCGTAGGCCGGATAAGGCGTTCACCCCGCATCCGGCAGTTGTCGCCCAATGCCTGAGGCCACGCTTGTCGCGTCTTATCATGCCAACATGCCTGGGCCGCACCGTAGACCGGCAAAAGCGTTCAcg                                                                                                                               >  2:315100/1‑238 (MQ=14)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                                                                                                                                               >  2:317325/1‑157 (MQ=255)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                                                                                                                                               <  1:317325/157‑1 (MQ=255)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaa                                                                                                                                                                     >  1:15058/1‑199 (MQ=255)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTTTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                             >  1:180665/1‑238 (MQ=255)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCCTAGGCCGGATAAGGCGTTCAcccc                                                                                                                             >  1:464130/1‑238 (MQ=255)
                                                                                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGCCTTTGCCGAACCGTAGGCCGGGTAAGGCGTTCAcgcc                                                                                                                             >  1:151437/1‑238 (MQ=255)
                                                                                          tAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTTTCGCGTCTTCTCATGCCTACAACGTTGTGCCGCACCGCAGGCCCGGAACGGCGTTCAccccccc                                                                                                                          >  2:553881/1‑236 (MQ=14)
                                                                                             cGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGa                                                                                                                                                                                                 >  2:50877/1‑166 (MQ=37)
                                                                                             cGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGa                                                                                                                                                                                                 <  1:50877/166‑1 (MQ=37)
                                                                                                gCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGc                                                                                                                                                                                                          <  1:785141/154‑1 (MQ=21)
                                                                                                gCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGc                                                                                                                                                                                                          >  2:785141/1‑154 (MQ=21)
                                                                                                           gATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACCCTTGTCCCGTCTTATCATGCCTCCAAGCTTGTGCCGACCCGAAGGCCGGAAAAGGCGTTACACCCGCACACGGCAGGCGGCGCaa                                                                                                         >  2:164417/1‑238 (MQ=14)
                                                                                                            aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCCCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTGTCGCCGCTTAGCATGCCTACAAGTCTGTGCCGAACCGCAGCCCCGATAAGGCGTTGACCACCCATCCGCCAGTGgcgcccaa                                                                                                       >  2:158778/1‑235 (MQ=14)
                                                                                                                  cGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGAAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGTTAAGGCGTTCCCGCCGCATCCGGCGGTCGGCGCATAAAGCCt                                                                                                  >  1:237133/1‑239 (MQ=255)
                                                                                                                           cgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGTGACGCTTGTCGCGTCTTATCATCCCTCACAGTCTGGGCCGAACCGTAGGCCGCATAAGGCGTTCACCCCCCATACGGCAGTCGGCGCATAAGGCCCGAcgcggc                                                                                          >  2:680915/1‑236 (MQ=14)
                                                                                                                                       tcggCGCATAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGCCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGCCt                                                                             >  1:108911/3‑239 (MQ=255)
                                                                                                                                            gcAAAATGCCTGATGCGACGCTTAACGGGTCTTTCCAGGCCTACAGGTTTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATc                                                                         <  1:588896/238‑1 (MQ=255)
                                                                                                                                             cacaAGGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCa                                                                        <  1:357671/238‑1 (MQ=255)
                                                                                                                                                  tGCCTGAGGCGACGTTTAACGCGTCTTACCATCCCTACATGTTTGGCCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTa                                                                  <  1:680915/239‑1 (MQ=255)
                                                                                                                                                                                   ccTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                                >  1:643027/1‑239 (MQ=255)
                                                                                                                                                                                       cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTTTCGCGTCTTTTCATGCCTACAGGTTTTTGCCGCACGTAGGCCGGATAAGAGGTTc                            >  1:19059/1‑238 (MQ=255)
                                                                                                                                                                                            ttGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCCTAATGCCTGATCCGACGCTTGTCGCGTCTGTTCCTGCCCACCGGTTTGTGCCCAACCCCAGGCCGCGAAAAGCGTCGcgccg                      >  2:227671/1‑239 (MQ=14)
                                                                                                                                                                                             tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGACGCATCCGGCAGTCGGCGCATAATGCCTGAGGCGCCGCTTGTCGCGTCTTATCATCCCGCCAGTTTGTGTCCGCACCGCAGGCCGCTAAAGGCGtcgccccca                      >  2:625580/1‑231 (MQ=14)
                                                                                                                                                                                                    aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAt                                                                                                                                                                             >  2:350090/1‑83 (MQ=38)
                                                                                                                                                                                                         tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                                               <  1:813177/139‑1 (MQ=255)
                                                                                                                                                                                                         tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                                               >  2:813177/1‑139 (MQ=255)
                                                                                                                                                                                                                gATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcc                                                                                                                                                >  2:655086/1‑100 (MQ=40)
                                                                                                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTCTCAGTCCTACAGGTTTGTGCCGAACCCTGGACCGGAAAAGGCGTGCCCGCCCCATACGGCAGTGGGCGcaaa  >  2:824614/1‑237 (MQ=14)
                                                                                                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCACGCCTACAGGTTTGGTCCGAACCGCAGGCCGGATAAGGCGTTCGCGCCCCCACCGGCAGTTGGCCcaca  >  2:569577/1‑239 (MQ=14)
                                                                                                                                                                                                                           cACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGt                                                                                                                                                                                          >  1:80554/1‑47 (MQ=35)
                                                                                                                                                                                                                                            ttGGCGCGCAATGCCTGAGGCGGCGCTTGTCGCGTCTTATCATGCCTACAAGTCTGAGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGt                                                                               <  2:842995/136‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                    
GCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACA  >  NC_000913/4614159‑4614605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: