Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,085 G→A 15.7% L29F (CTC→TTC)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0850GA15.7% 129.4 / 2.2 59L29F (CTC→TTC) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (29/20);  new base A (3/7);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTG  >  NC_000913/751847‑752303
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                          
attccccGATTTTTCTTGGGCAAGGGCTTAATGATATCTGGTGTTAAACCAAATTTATCGACCAGTGAAACATTGATGCATGGAATGGTTTTGTCGCCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                                                                                                            <  2:243020/234‑1 (MQ=255)
        atctatctGTGGCAAGGACGGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGCATCATTTATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCGTTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATGTTCACCGCGAGCAATGCCTCCTTTTTCCTTTCATAAGGAAATGTCAATGCGGTCCGACAGTTATTTATCGGAACCATTCa                                                                                                                                                                                                                    >  1:886523/1‑239 (MQ=255)
          ctatctGGGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGa                                                                                                                                                                                                                  <  1:879420/239‑1 (MQ=255)
               tgtgGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATTCGGTCGCGCATTGCTTTATCGAGAACATTCAGATTGaa                                                                                                                                                                                                             >  1:429906/1‑239 (MQ=255)
                gtgGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTACCGAGAACATTCAGATTGAAt                                                                                                                                                                                                            >  1:791171/1‑239 (MQ=255)
                  ggCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAAAAAATTAAAATTAAATTc                                                                                                                                                                                                          >  2:53658/1‑239 (MQ=255)
                   gCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCCCATTGATTTATCTAAAAAATTCAAATTGAAttag                                                                                                                                                                                                         >  2:391064/1‑237 (MQ=255)
                       ggACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGAcc                                                                                                                                                                                                      >  1:709917/1‑238 (MQ=255)
                                      tttttttAAACCAAATTTTTCGACCTGGGAATCTTTGTTGCGTGGAATGGTTTTTGCCCCTTTTTTTTCCCAGTTAATTTTTTCCCCATTACTGTTTTTTGTGTTAGTCCCCGCAACGTAAACAAAATTTTCCCGGGGAGCAATGACTCCTTTTTCCTTTAATAATAAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCa                                                                                                                                                                                      <  2:709917/236‑1 (MQ=255)
                                      tttttttAAACCAAATTTATCGGCCAGTTAACTATTGATTCATGGAATGTTTTTGTCCCCCTTTTTTTCCCAGTTAATTTTTTGCCCATTCCTGATTTTTTTTTTATTCCCCGCAACGCTAACAAAATATTCCCCGGGGTCAATGCCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCa                                                                                                                                                                                      <  2:648067/236‑1 (MQ=255)
                                      tGGTTTTAACCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGTTTTTGTCACCTTTTTTTGGCCATTTAATTTTTTGCCAATTACTGATTTTGTTGTTATTCCCCGAACAGGTAACAAAATATTCCCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCa                                                                                                                                                                                      <  1:65635/239‑1 (MQ=255)
                                           ttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCCCCATTAACCAAGGCATCGcc                                                                                                                                                                                 >  2:558211/1‑239 (MQ=255)
                                             aaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAgg                                                                                                                                                                                        >  1:419477/1‑230 (MQ=255)
                                                    aTTTATCGACCAGTGAATCATGGTGGCATGGAATGGTTTTGTCCCCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcagg                                                                                                                                                                        <  1:81818/239‑1 (MQ=255)
                                                      ttatgagacAGTGAATCATTGATGCATGGAATGGTTTTGTCCCCCTTTTTTTGGCAGTTAATTTTTTGCCATTTACTTATTTTGTTGTTTTGCACCGCAACGCTAAAAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAAAAATGAAATATCAATGCGGTCGCGCAGTGATTTGTCGAGAACATGCAAATGGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                                                                                                                      <  1:215425/231‑1 (MQ=255)
                                                           gACCAGTGAATCATTGTTGCATGGTATGGTTTTGTCACCCTTTTTTTGCCATTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACCATAACAAAATATTCCCCGGGAGCATTGCCTCGTTTTGCCTTTAAGAATGAAATATCAATGCGGCCCCGCATTGATTTATCGAGAAAATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATa                                                                                                                                                                 <  1:14102/239‑1 (MQ=255)
                                                                 tGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCACATTGATTTATCGAGAACATTCAGATTGAATTCCACCGCCATTGCGCAAGGCATCGACATTACCAGGCAGGAAACaaaaa                                                                                                                                                           >  2:807936/1‑238 (MQ=255)
                                                                           tttgaTGGATTGTTTTTGTCTCCTTTTTTTTCCCATTTATTTTTTTGCCTTTTATGTGTTTTGTTTTTATTACCCACACCTCTAAAAAAATATGCACCGGGGTCAATGACTCCTTTTTCTTTTAATAAGGAAATATCAATGCGGTCGCGTATTGATTTATCGAAAACATTCAGATTAAATCGGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                                                                                                                 <  2:726363/235‑1 (MQ=255)
                                                                           ctgattGGAATGTTTTTGTCCCCCTTTTTTTGCAAGTTATTTTTTTGCCAATTATTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCCCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                                                                                                                 <  1:229598/234‑1 (MQ=255)
                                                                           aTGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                                                                                                                 >  1:69868/1‑239 (MQ=255)
                                                                           aTGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                                                                                                                 >  1:809337/1‑239 (MQ=255)
                                                                                    tttgTTTTTTCTCCCTTTTTTTGCCATTTAATTTTTTGCCCATTCAGGTTTTTGTTGTTATCCCCCGCAACGCAAAAAAAATATTCACCGGGAGAAATGACTCTTTTTCCCTATAATAATAAAATAACAATGCGGTCGCGCATTAATTTACCGAGAACATTCAGATTGAATACGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                                                                                                                        <  2:429906/236‑1 (MQ=255)
                                                                                     tGGTTTTTTCCCCCTTTTTTTGCCAGTTATTTTTTTGCCCATTACTGATTTGGTTGTTATTCCCCGAAACGTTAAAAAAATATTCACCGGGAGCAATGCCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGAATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:391064/239‑1 (MQ=255)
                                                                                     tGGTTTTGTCCCCTTTTTTTTGCCAGTTAATTTTTTGCCAATTACTGATTTTGTTGTTATTCACCGAAACGCTAACAAAATATTCACCGGGATCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:803446/239‑1 (MQ=255)
                                                                                     tGGTTTTGTCCCCCTTTTTTTGCCAGTTAATTTTTTGCCATTTACTAATTTTGTTGTTATTCACCCAAACCCTAAAAAAAAATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAAGGAAATATCAATGCGGTCGCGCATTGATTTATCGAAAACATTAAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:558211/239‑1 (MQ=255)
                                                                                     tGGTTTTGTCCCCCTTTTTTTGCCAGTTAATTTTTTGCCAATTACTGATTTTTTTGTTATTCCCCGCAACGCTAACAAAATATTCCCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:598699/239‑1 (MQ=255)
                                                                                      ttataggTCCCCCTTTTTTTGGCCATTTATTTTTTTCCCATTTTCTGATTTGTTTTTTTTTCACACCAACGCTAAAAAAATATTCACGGGGGACAATGACTCTTTTTTCCTTTAAAAAAGAAATATCAAGGCGGTCGCGCATTGATTTATCGAAAACATCCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  2:476835/233‑1 (MQ=255)
                                                                                       gTTTTGTCCCCTTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCCCCGCAACGCTAAAAAAATTTTCACCGGGAGCAATGCCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:779655/237‑1 (MQ=255)
                                                                                             tCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAACTATTCACCGGGAGCATTGAATCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGAGCAAGGCATCGCCATGACCCGGCCGGGGACAAAAGCGATTCGATAAAAATTACCGGGGTCCAt                                                                                                                               >  1:410958/1‑239 (MQ=255)
                                                                                             tCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCa                                                                                                                                >  1:627751/1‑238 (MQ=255)
                                                                                             tCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAAACAAAAGAGAGTCGAAAAAAATTccaggggcccaa                                                                                                                               >  2:274174/1‑228 (MQ=255)
                                                                                               tcctttttttttCCTTTTTATTTTTTCCCCTTTGCATTTTTTTTTGTTTTTCCCCCAAACGTTAAAAAAATTTTAACCGGGAACAAGTCCCTCTTTTTCCTTAAATAATGAAATATCAATGCGGCCCACAATTGATTTACCGGGAATATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATAc                                                                                                                             <  2:791171/235‑1 (MQ=255)
                                                                                                      ttttGCCAGTTAATTTTTTGCCAATTACTGATTTTGTTGTTATTCACCGAAACGCTAACAAAATATTCCCCGGGAGAAATGCCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGAATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATaa                                                                                                                      <  1:236609/239‑1 (MQ=255)
                                                                                                            ccAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAAAGTCGATAAATATTCACGGTGTCCATAc                                                                                                                             >  1:476835/1‑227 (MQ=255)
                                                                                                                ttAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATGTTCACCGGGCGCATTGACTCCTTTTTCCTTAAATAATGAAATATCAATGCGACCGCGCATTGATTTATCGAGACATTTCAGATTGAATTCGACCGCCATTGACCAAGGCATCGCAATGACCAGGCAGGCTACAAAAGAGAGTCGATAAGACTTCACGGTGCTAATACCTGATAAATATTTTAt                                                                                                             >  2:620334/1‑239 (MQ=255)
                                                                                                                       ttggCCAATTCCTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCCCCGGGAACAATGACTCCGTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggc                                                                                                      <  1:97763/236‑1 (MQ=255)
                                                                                                                               ttACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatga                                                                                              >  1:179109/1‑239 (MQ=255)
                                                                                                                                tACTGATTTTTTTGTTATTCACCGCAACGAAAACAAAATATTCCCCGGGAGCAATGACTCCTTGTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTAACGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACg                                                                                                                                       <  1:474268/197‑1 (MQ=255)
                                                                                                                                      ttttttttttATTTCCCGCAAACTCAAAAAAAATTTGCCCCGGGAGAAATATCTCGTTTTCCCTTAAATATGAAAAAATAAACGGGGCCGCGTATTATTTTAGCGAGAAGCTACAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATgccgc                                                                                        <  2:809337/239‑1 (MQ=255)
                                                                                                                                        ttgttgTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGGGTCCATACCTGATAAATATTTTATGAAAAACGGCGATGATTCCCCCaa                                                                                     >  2:489498/1‑239 (MQ=255)
                                                                                                                                                aTTCCCCGCAACGCTAACAAAATATTCCCCGGGAGCAATGCCCCCTTTTACCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAAAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATAc                                                                             <  1:489498/239‑1 (MQ=255)
                                                                                                                                                aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAaataaa                                                                               >  1:400445/1‑236 (MQ=255)
                                                                                                                                                      cGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGGGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACttattt                                                                       >  1:353453/1‑239 (MQ=255)
                                                                                                                                                        caacctatAAAAAAATTTTCCCGGGGGGAAAGTATCTTTTTTCCTTTAAAAAATAAAATACAAATGCGGGCCCGAATGTATTTATGGAAAAAATCCAGTTAGAATTCAACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACttattta                                                                      <  2:179109/232‑1 (MQ=255)
                                                                                                                                                        cAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATACTACttatttat                                                                     >  1:347550/1‑239 (MQ=255)
                                                                                                                                                            cgtgaaaaaaaTATTCACCGGGAGCAATGCCTCTTTTTTCCTTTAATAATGAAATATAAATGCGGTGGCGGTTTGATTTATCGAAAACTTTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAAt                                                                  <  1:274174/232‑1 (MQ=255)
                                                                                                                                                               tAACAAAATATTCACCGGGAGCAATGCCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTACCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATcc                                                                <  1:53658/238‑1 (MQ=255)
                                                                                                                                                                 aCAAAATATTCACCGGGAGCAATGAGTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGCCAGGTTGAAAAAGAGAGCCGATAAAAATTCCAGGTTTCAATACCTGATAAATATTTTTTGCAAGGCGGCAATAATGCCCCCAAAATAAAATTTATTTTAAATCCaa                                                              >  2:147174/1‑237 (MQ=255)
                                                                                                                                                                 aCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAAAATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGAGGCGATGATGCCGCCAAAAAAACACtttttttaataccaac                                                             >  2:831480/1‑229 (MQ=255)
                                                                                                                                                                   aaaaTATTACACCGGGAACAATGACTCATTTTTCCTTAAATAATGAAAATTCAAGGCGGTCGCGATTTGATTTACCGAGAACATTCATATTGAACTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAGTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCa                                                            <  1:620334/239‑1 (MQ=255)
                                                                                                                                                                            cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGCCAGGATACAAAAGCGAGTCGATAAATATTGACGGTGTCCTTACCTGATAACTATTTTATGAAAGCCGGCGTGGATGCGCCCAAACAAATACTTTTTTTTAAACCAGCAccacgggag                                                   >  2:773454/1‑231 (MQ=255)
                                                                                                                                                                                        tGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGAGGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGtt                                        >  1:168916/1‑238 (MQ=255)
                                                                                                                                                                                             ccTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAACGGTTACTTc                                   >  1:869137/1‑238 (MQ=255)
                                                                                                                                                                                                ttttCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAACAGCGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTAAcc                                >  1:397312/1‑238 (MQ=255)
                                                                                                                                                                                                   tCCTTTAAAAAAGAAATATGAGGGCGGTCGCGCTTTTATTTACGAAAAATATTAAGTTTATTTGCGCCCGCCATTGCGCAAGGCATCGCCATGACAAGGCAGAATAAAAAAGAGGGTCGATAAATATTCACGGTGCCCATACCTGAAAAATATTTTAGGAAAGGCGGCGATGATGCCGCAAAAAAAATCCTTATTTATAATCCAGCACGTAGGTTGCTTTAGCGGTTACTTCCCCTGcc                            <  2:869137/239‑1 (MQ=255)
                                                                                                                                                                                                        taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                                                                                                             >  1:114083/1‑153 (MQ=255)
                                                                                                                                                                                                        taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAACGTCGGCGATGATGCCGCAAAAGTAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCCCCTGCCGCGAc                       >  1:502066/1‑239 (MQ=255)
                                                                                                                                                                                                        taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGAc                       >  1:716318/1‑239 (MQ=255)
                                                                                                                                                                                                        taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCACAAATAATACTTATTTATAATCCAGCCCGTAGGTTCCGTTACGGGTGACTTCACCTGCCGTGAc                       >  1:254211/1‑239 (MQ=255)
                                                                                                                                                                                                                              gTCGCGCATTGATTTATCGAGAATATTCAGCTTGAATTCGACCGCCATTGCGCAAGGCATCGCCATTACCAGGCAGGATACAAAAGAGAGTCGATAACTATTCACGGTGTCCATACCTGCTAAACATTTTATGAAAGCCGGCGATGATCCCGCCAAAATCATACTTATTTTTAATCTAGACCCTAGCTTGCGTTAGCGGTTACTTACCTTGCCGTCAGACCGACTGCattttgattttg  >  2:614654/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 agaACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACttattta                                                                      >  1:136436/1‑152 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                          
ATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTG  >  NC_000913/751847‑752303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: