| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 74,046 | A→G | 18.0% | L159P (CTC→CCC) | thiP ← | thiamine/thiamine pyrophosphate ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 74,046 | 0 | A | G | 18.0% | 173.1 / 2.9 | 72 | L159P (CTC→CCC) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (13/43); new base G (12/2); total (27/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCG > NC_000913/73810‑74283 | aGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCGAGTTGGCGCAGGTCGCCG‑GGGATGTTTTCCAGGGCCTGGGGt > 1:454307/1‑239 (MQ=255) cAGCGCCTGAAAGATTGCCAGCGCGAAAGTGGTCGCCTGCGGACCGCCCCGCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGGGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGc < 1:68699/239‑1 (MQ=255) aGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTCACCACCGCCAATCGCCGACACCGAAAAAATGCAAGCTACGCCTCCCAACCTAGGCGGCAAGTTGACGCTGTTCGCCGCGGGAGTTTTTCCAG‑GCCTGGGGgaataaaaggc > 2:541477/1‑233 (MQ=255) aGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGACGGGATTTGTCGCCGCAACCACGGCCATTCGACGAAGCGGAAAAAATACCAGCTCCGCATCCCAAGCAGGGCGGCAAGGTGACGCCATTCGCCG‑GGGGTGGTTTCCAGGGCAGGGAGtacaaaggggat > 2:68699/1‑229 (MQ=255) aTAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACCCGGTTCCCCG‑GGGAGGTTTTCCAGTGCCTGGGGTAATAAACGGCCCCCCATCg > 1:336906/1‑239 (MQ=255) aGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCgg < 2:919930/238‑1 (MQ=255) gATTGCCAGCTCGATAGGGGTCCCCGGGCGACCGCCCCCCAGCGATAGCCCGGTGGCGAAGCTGGCAAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCa < 1:52812/239‑1 (MQ=255) agggacaggCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCa < 1:841594/231‑1 (MQ=255) gCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCCTAACCACGGCCATTCGCCGAAGCGGCAAAAATGCCAGCTACGCATCCCCAGCTGGGCGGCAAGTTGACGCTGTTTCCCC‑GGGGTGTTTTCCCGTGCCCGGGGAAAAAAGCGGCGGCCCACCGGCA‑GAGGaaaa > 2:52812/1‑239 (MQ=255) gCTCGATAGGGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGGGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTaaaa < 1:1085449/239‑1 (MQ=255) cTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCCAGTTGACGCTGTTCGCCG‑GGGAGGTTTTCCAGTGCCTGGGGTAATAAGCGGCTCGCCATCGGCA‑GATTaaaaa > 1:179273/1‑239 (MQ=255) tggggcgcccTGCGGGCCCCCCCCCCGCGATGGCCCGGTGGCGAAGCCGGGTAAAAGGAGCAAAAAGTATAGCGCAGCAACCGGCGGGATTTGGCGCCGTAACCCCGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca < 2:142454/230‑1 (MQ=255) aggggccggcTGCGGCCCCCCCCCCAGCGATAGCACGGTGGCGAAGGTGGCGAAACAGAGCATAAAGATAAGCCCAGCAACCGGCGGGATTTGTCGCCGTAACCCCGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca < 1:1092837/229‑1 (MQ=255) aGTGGCCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca < 1:406512/238‑1 (MQ=255) gtccccgcgggACCGCCCCCCGGCAGTAGCCCAGTGGCGAAGTTGGCGAACCAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGTCCATTCGACGAAGCGGAAAAAATGCCCGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGc < 2:372471/230‑1 (MQ=255) gTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGGTTTCCAGTGCCTGGTGTCATAAGCGGCCCGCCATCGGCC‑GCTTTAAAAAaacactggc > 1:435122/1‑234 (MQ=255) gTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGGAACCACGGCCATTCGACGAAGCGGAAAAAAAGCCAGCTACGCATCCCCAGCTGGGCGGCAAGTTGACGCTGTTTCCCG‑GGGAGGTTTTCCAGTGCCTGGGGAGAAAAACGGCCCCCCACCGGCA‑GGTTAAAAAaaacgtctct > 2:841594/1‑233 (MQ=255) tCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGc < 1:493083/238‑1 (MQ=255) gcGCCGGCGGCCCGCCCCCCAGCGATAACACGGTGGCGAAGGCGGTGAAACAGACGATAAAGAGAAGCGCAGCACACGGGGGGATTTGTCGCCGTAACCACGGCCATGTGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGTCGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGc < 2:611590/237‑1 (MQ=255) ccgACCGCCCCCCAGGGATAGCCCGGTGGCGAAGCTGGGGAAACAGAGACAAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑Caaaa < 2:59781/237‑1 (MQ=255) cGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCCGCTACGCATCCCAAGCTGGGCGGCAAGTTGACCCTGGTCGCCG‑GGGAGGTTTTCCCGAGCATGGGGTAATAAGCGGCCCCCCCTCCGCAGGTTTAAAAAACACA‑GGGCCCG‑Aaaaa > 2:1035525/1‑239 (MQ=255) cGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGCTGTTTTCCAGTGCCTGGAGGAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCt > 1:967334/1‑239 (MQ=255) ccccgcgCGAGAGAACGGTGGCAAAGTGGGCTACACATAGCATAAAGAGACGCGCAGCACCGGGCGGGATTTGCCGCCGTAACCCGGGCCAGTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCGGGGGGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATGCCTTGc < 2:937788/233‑1 (MQ=255) tAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGGGTAATAAGCGGCTCGCCATCGGCA‑GCTTAAAAAACACATGGGCCAG‑CAAAAAACCTTTCAGGCCGTAgg > 1:238295/1‑239 (MQ=255) tccgcGGGGGGGAAGGTGGCGAACCAGAGCAAAAAGATAAGCGCAGCGCCGGGGGGGGTTTGGAGCCCTACCCACTGGCAATCGACAAAGCGGAAAAAATGGCAGCGACGCATCCCAAGCTGGGGGGCAAGTGGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAT‑CAAAATACCGTGCAGGCCGTAgg < 2:224511/234‑1 (MQ=255) gCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGGGTAATAAGCGGCTCGCCATCGGCA‑GGTTAAAAAAAACACGGGCCAG‑CAAAAAACCTTGCAGGCCGTAaggg > 1:522274/1‑238 (MQ=255) ggaggCTGGGAAAACAGGGCATAAAGGAAAGCGCAGGAACCGGGGGGTTTTGTCGGCGTAACCCCGGCCATTCGACGAAGGGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGGCGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGt < 2:546229/234‑1 (MQ=255) ggAAGCTGGCGAAACAGGGAAAAAAGATAAGCGAAGAACCCGGCGGGATTTGTCGCCGTAACCCCGGCCATGCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGt < 2:1060563/237‑1 (MQ=255) ctaaGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGt < 1:192428/236‑1 (MQ=255) cGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGTATTTGTCGCCTTAACCACGGCCTTTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGt < 1:90226/238‑1 (MQ=255) cGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGt < 1:819234/238‑1 (MQ=255) aaGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAAGGCCAGCTACGCATCCCCAGCTGGGCGGCAAGTTGACGCTTTTCGCCG‑GGGATGTTTTCCCGGGCCTGGCGGGATAAGCGGCCCGCCATCGGGC‑GATTAAAAAAAACATCGGCGAG‑CAAAATACCTTGCAGGCCGAGGGGGGCAAAgaaca > 2:192428/1‑234 (MQ=255) aGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCACGCAGGGCGGCCAGTTGACGCCGTTCGCCG‑GGGATGTTTTTCAGTGCCTGGGGTAATAACCGGCTCGCCACCGGCC‑GGTTAAAAAACACCAGGGCCAGCCAAAAAACATTCCGCGCC‑TAGGGGGAAAAggcacac > 2:1038925/1‑234 (MQ=255) ggCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTTCAGGCCGGAAGGCGAAAAAGTCCACCCCa > 1:1056249/1‑239 (MQ=255) aCAGAGCATAAAGATAAGCGAAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGa < 1:638188/239‑1 (MQ=255) gCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGGGTAATAAGCGGCTCGCCATCGGCC‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGGGAAAAGGTCCACTCCAGAccagccgat > 1:305805/1‑232 (MQ=255) gCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca > 1:576557/1‑179 (MQ=255) gCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca < 2:576557/179‑1 (MQ=255) gCATAAAGATAAGCGCAGAAGCCGGCGGGATTGGTCGCCGTACCCACGGCCATTCGACGAAGCGGAAAAAGAGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGc < 1:1036678/236‑1 (MQ=255) aaaGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGc < 1:1004320/239‑1 (MQ=255) aGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAAAgggt > 1:606889/1‑239 (MQ=255) aCCGGGGGGATTTGGCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgccagcca < 1:284452/239‑1 (MQ=255) cggGTTTTCTCGCCGTAACCACGGCCATTCGCCGAAGCGGAAAAAATGCCAGCTGCGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCt < 2:1106928/239‑1 (MQ=255) gggATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGAAGGGCGAAAAGGTCCACCCCAAACCGAGCGATTGGCAGAGTGTTGCCAGCCAGcccgg > 1:745890/1‑237 (MQ=255) tttGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCg < 1:435599/238‑1 (MQ=255) tttGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCg < 1:217155/238‑1 (MQ=255) tttGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGGTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGGGTAATAAGCGGCTCGCCATCGGCC‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGGACACTCCAAACCACACGACTGGCAGAGGGTTTCCACCCAACCCCgggc > 2:45298/1‑236 (MQ=255) cGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATa < 1:630110/239‑1 (MQ=255) cGCCCTAACCCCGGCCTTTCGGCGAGGCGGAAAAAATGCCAGCTACGCTTCCCAAGCGGGGGGGCGAGTGGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACCCATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATa < 2:454307/239‑1 (MQ=255) gCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGc > 2:302483/1‑136 (MQ=255) gCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGc < 1:302483/136‑1 (MQ=255) ttCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGa > 1:27558/1‑239 (MQ=255) tCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTTCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAACCATCGGCCAG‑CAAAATACCTTGACGGCCGCAGGGCGAAAAGGGCCACTCCAGACCGAGCGATTGGACGAGGGTTGTCCCCCCGCCCTGGCGACCACAGACACCAAGaagaccaaa > 2:585493/1‑232 (MQ=255) cgacgaAGCGAAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGaaa > 1:776337/1‑239 (MQ=255) cgaAGGGGAAAAAATGCCGGCTACGATCCCCAAGGGGGGGGGAAAGTTTGCGGTGTTCGCCG‑GGGAAGTTTACCAGTGCCTGGAGTAATAAGCGGCTCGCCAGCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACa < 2:435122/239‑1 (MQ=255) ggAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGAc < 1:45298/238‑1 (MQ=255) ggAAAAAAGGCCAGCTACCAATCCCAAGTGGGGCGGGAAGTTGACGGTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGAc < 2:606889/238‑1 (MQ=255) aaaaaGGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGAGGATGTTCGCCG‑GGGATGTTTTCCAGTGCCGGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCaa < 1:1038925/238‑1 (MQ=255) gCCAGTTACGCATCCCAAGTGGGGCGGAAAGTTGACGTGGTTCGCCG‑GGGATGTTTTCCAGGGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCggg < 2:776337/239‑1 (MQ=255) ctggtACGCATCCCAAGCTGGGCGGCAAGTGGACGCTGTTGGCCG‑GGAATGCTTTCCAGTGCCTGGATTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAGACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGgga < 1:1064436/234‑1 (MQ=255) cAGCTCCGCATCCAAAACTGGGTGTCAAGGTGACCCTGTTCGCCG‑GGGATGTTTTCCAGTGACTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACTTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGgga < 1:913933/238‑1 (MQ=255) tACGCCTCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATTCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGAt < 1:1103626/238‑1 (MQ=255) tGGGGGGCAAGTGAGCGCTGTTCGCCG‑GGGATGTTTTCACGGGCCGGGGAGAATAAGGGGCTCGCCATCGGGA‑GGTTAAAAAACAAATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGcac < 2:336906/238‑1 (MQ=255) gggggcAAGTGGGCGCTGTTCGCCG‑GGGTTTTTTTACAGTGCCTGGAGTAAGAACCTGCCCGCCAGCGGCA‑GATTAAAAAACCCATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGcacac < 2:305805/235‑1 (MQ=255) ttCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca > 1:905321/1‑67 (MQ=255) ttCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAAcaca < 2:905321/67‑1 (MQ=255) ttCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGc < 2:497201/155‑1 (MQ=255) ttCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGc > 1:497201/1‑155 (MQ=255) tttCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAgcgc < 1:895024/239‑1 (MQ=255) tttCCAGTGCCGGGGGTAAAAAACGGCTCGCCATGGGAA‑AATTAAAAAACCCAGGGGCCAG‑CAAAATTCCTGGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAgcgc < 2:522274/239‑1 (MQ=255) ttcaggggCCTGGAGTAAAAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGGGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGcc < 1:585493/232‑1 (MQ=255) ggAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAAACGCGACCAACACCGGGAGGAGCAAGGTCACTGCACCCAAACCAAAAAGCGCCACCCGCCCCCGGAAGCGCCTGCGaacaagc > 2:304624/1‑233 (MQ=255) aGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATagaacc > 1:725313/1‑235 (MQ=255) | AGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCG‑GGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCA‑GATTAAAAAACACATGGGCCAG‑CAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCG > NC_000913/73810‑74283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |