Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,435,706	T→A	13.0%	intergenic (‑87/+54)	uspF ← / ← ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,706	0	T	A	13.0%	175.6 / 2.9	66	intergenic (‑87/+54)	uspF/ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base T (54/2);  new base A (2/7);  total (57/9)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435485‑1435940                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATTACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTAAAT‑TTAGATAAAAGGGGGAAGAaaaa                                                                                                                                                                                                                           >  2:399098/1‑239 (MQ=255)   gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCACAGGAGGAAGAATAAGc                                                                                                                                                                                                                         >  1:1024639/1‑239 (MQ=255)    cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAAAGAGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATAT‑CGAAAGGGACAAGAAACGGTCTGTTCATAAAA‑ACTCCTGTATTAGTATCCGCATAAAGTGAA‑ACGCCAGAGGAAACTTTTTTGGA‑‑AAAGAC‑GGGGTAAAAATGTTTAAT‑TTAAAAAAAAGGAGGAAGAAAAAACa                                                                                                                                                                                                                        >  2:604244/1‑239 (MQ=255)      gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGTAAATAT‑CGATAGGGACAAGAACCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGAATCCGCATAAAGTTAA‑ACGCCCGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGAAGGAAAAATAAGCaaa                                                                                                                                                                                                                      >  2:532745/1‑237 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCCTAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑CAGCCCGATTACACTTTTTTTGT‑‑AA‑GACGGGAGTTCACATTTTTAAT‑TTAAGACAAAGGAGGAAAAATAAGcgaaa                                                                                                                                                                                                                     >  2:979297/1‑235 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAgaagaa                                                                                                                                                                                                                     >  1:1093464/1‑239 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGGGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGTAAGAATAAgacgaa                                                                                                                                                                                                                     >  1:555980/1‑234 (MQ=255)         aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGGGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGaaaa                                                                                                                                                                                                                   >  1:104927/1‑239 (MQ=255)            gTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTTTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAg                                                                                                                                                                                                                 >  1:471070/1‑238 (MQ=255)                tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGc                                                                                                                                                                                                            >  1:219085/1‑239 (MQ=255)                     tCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGTTCAATGGAGGAAGAATAAGCAGAAAAACCCCCCCATa                                                                                                                                                                                                        >  2:623170/1‑238 (MQ=255)                       aGGAAATGCACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGCTGAGTTAATTCTGAATCGGAAATAT‑CGACAGGTACACGCATCGCTCTGTTCATAAAA‑CCTCCGGTTTTAGAATCCCCAAAAAGTCTA‑ACGCCAGCTGGCACTTTTTGTGT‑‑AATAAC‑GGAGTTCACATTTTTAAT‑TTAAACCCAAGGCGGCAACATAAACAACAAAACCCCCCAATaaaa                                                                                                                                                                                                     >  2:789356/1‑237 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAATTAT‑CGATAGGGACAAGAGTCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTTTGT‑‑AATGAC‑GGCGTTCCCATTTTTAAT‑TTATATCAAAGGAGGAAGAATAACAAAAAAAAGCCCCCCAAAACa                                                                                                                                                                                                     >  2:412392/1‑239 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGCGGAAGAATAAGCAGAAAAAGCCCCCCATaaa                                                                                                                                                                                                      >  1:625926/1‑237 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACa                                                                                                                                                                                                     >  1:552642/1‑239 (MQ=255)                                       catcatCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTAACATTTTTAAT‑TTAGATCAATGAAGGAAGAATAAGCAGAAAAAGACACCCAAAAAACCGGGCagagagattt                                                                                                                                                                                     >  2:97201/1‑231 (MQ=255)                                            tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGAGAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGCTGACACTTTTTGTGT‑‑ACTGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGAAGGAGGAGTTAGAAc                                                                                                                                                                                >  1:1037971/1‑239 (MQ=255)                                            tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAACAGAAAAAGCCCGCCCTAACACCGGGCAGGAGGATTTAGAAc                                                                                                                                                                                >  2:1033537/1‑239 (MQ=255)                                                    cgCCTCTCCCTCAACGGGGAAAATCACGGGTTGTATTAATTCTGAA‑CCGAAATGTGCGAAAGGAACAAGAATCGTTCTGTTAAAAAAC‑CCCCCTGTTTGAGTACCCGATAAAAGTGAA‑CCGCAAGAGGACCTTTTTTGTTA‑‑AATGAG‑GGAGTTCACATTTTAAAT‑TAAGATCAAGGGAGGAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                                                                                                                                        <  1:623170/238‑1 (MQ=255)                                                     gCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGCGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATATGCAGAAAAAACCCGCCATAACAGCGGGCAGGAGGGTTTAAAAACGAAAAAca                                                                                                                                                                       >  2:478216/1‑238 (MQ=255)                                                      ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAAAGCGGGCAGGAGGATTTAGAAATGAAAAAcaa                                                                                                                                                                      >  2:180703/1‑237 (MQ=255)                                                          ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCCTAACAGCGGGCAGGAGGATTTAGAACTGATaaaccaaacc                                                                                                                                                                  >  1:432727/1‑239 (MQ=255)                                                                aaCGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCCGAAAAAGCCCGCCATAAAAGTGGGCAGGGGGGTTTAGAACTGAAAAACCAgcaccaaaac                                                                                                                                                            >  2:710805/1‑230 (MQ=255)                                                                    tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGCGTA‑ACGCCAGATGACACTTTTTTTGT‑‑AAGGAC‑GGAGTTAACATTTTTAAT‑TTAGATCAAag                                                                                                                                                                                                                                       >  2:319405/1‑160 (MQ=255)                                                                      gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      >  1:253979/1‑239 (MQ=255)                                                                       ttaatcctggtttGATTTATTTCTCAACGAGAATTAG‑CGATAGGGAAAAAAATCGTT‑TCTTCAAAAAACCCTCTTTTTTTATTTACCGAAAAAAGTGAA‑ACGCCAGATGAC‑TTTTTTGTTTA‑AAGGAC‑GGATTTATCATTTTTAAT‑TAAGAAAAAGGGAGAAAAAATAAGCAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:859144/227‑1 (MQ=255)                                                                        taaaactcgtttGTGTTTATTTTTAAGCGGAAATTT‑CGAAAGGGAAAAGAGTTTTTTTGTTAAAAAAC‑CCTCTTTTTTTTGTCTACCCA‑AAAATGTATAAGCCA‑AAGAGACCTTTTGGGTAAAAGGGG‑GGAGTCCACTTTTTAAAT‑TTAGAAAAAGGGGAAAAGAAAAAGAAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:601421/228‑1 (MQ=255)                                                                                cgTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACATCGGCAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:606007/1‑239 (MQ=255)                                                                                cgTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:882729/1‑239 (MQ=255)                                                                                   tGATTTATTTCTAAAGCGAAAATAT‑GAATAGGCAAAAAAATCTTTCTGTTAATAAAC‑CCCCCTTTTTAAGTATCCAAAAAAAGTGAA‑CCGCAAGATGACTCTTTTTGTGT‑‑AATGAC‑GGAGTTCATATTTTTAAT‑TTAAAAAAAAGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                                                                                                                                         <  1:180703/239‑1 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgg                                                                                                                                              >  1:338971/1‑222 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                                                                                                                             >  1:714493/1‑239 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAACTAAAGCGAAAATAACACAGGTCGAGATGACAATggaa                                                                                                                             >  2:238290/1‑237 (MQ=255)                                                                                                aaTCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                                                                                                                            >  1:256476/1‑239 (MQ=255)                                                                                                      aaaTAT‑GGAGGGGAAAAAAAATTGTTCTTTAAAAAAAC‑CCTCTTTTTTTAGTTCCCAAAAAAAGTTAA‑CCGCAAGATGA‑ATTTTTTGGGTA‑AAGGAG‑GGAGTTCACATTTTAAAT‑TAAGAAAAAAGGAGGAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg                                                                                                                       <  1:604244/238‑1 (MQ=255)                                                                                                             cGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAAATGCCATTGGCAGCGTAGAAGcagc                                                                                                                >  1:463793/1‑236 (MQ=255)                                                                                                                         gAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCAACAATACCAACGGGAAAGAAGCCATTGGCAGCGAAAAAACGGTCAACttacacca                                                                                                    >  2:885005/1‑233 (MQ=255)                                                                                                                             cGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAACGAAAAAACTGTAAAATTCATCCAAAAg                                                                                                >  2:374573/1‑239 (MQ=255)                                                                                                                               ttCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGc                                                                                                                                                                                               >  1:930785/1‑142 (MQ=255)                                                                                                                               ttCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:539514/1‑239 (MQ=255)                                                                                                                               ttCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGGGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGCAGCATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTATAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:711003/1‑239 (MQ=255)                                                                                                                                 cTGTTCATAAAA‑CCTTCTGTTTGAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGCGTTCACATTTTTAAT‑TTATATCAAAGGAGGAAGAATAAGCAGACAAAGCCCGCAATAACAGCGGGAAGGAGGATTTCGACCTGATAAACAAGAACTAAAGCGAAACTATCATCGGTAGAGATGCCATTGGCAACTAAAAAGGAGTCATCTTCAACCAAAAGGTTg                                                                                            >  2:794111/1‑239 (MQ=255)                                                                                                                                            a‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc                                                                                 >  1:678399/1‑239 (MQ=255)                                                                                                                                                  ctGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCAGTCATCTCAATCCAACAGGTGGATTTTATAGTCAACga                                                                            >  1:232079/1‑238 (MQ=255)                                                                                                                                                           tATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAATCTGTCATCTTCATCCAACAGGTTGATTTTATATCAAAAGAAGGTGGaaa                                                                   >  2:518043/1‑237 (MQ=255)                                                                                                                                                            aTCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGAAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGTTTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:986202/1‑239 (MQ=255)                                                                                                                                                               cGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa                                                                                                                                                                                 >  1:726683/1‑125 (MQ=255)                                                                                                                                                               cGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGCCATGtt                                                               >  2:869/1‑239 (MQ=255)                                                                                                                                                               cGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                               >  1:760898/1‑239 (MQ=255)                                                                                                                                                               cGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAGGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGACAACGAAGggggcaaagtt                                                               >  2:742410/1‑232 (MQ=255)                                                                                                                                                                         gtA‑ACGCCAGCTGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGTGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACCGATAAACCAGACCTAAAGCGACAATATCATCGGTATGGATGCCATTGGCACGATAGAAACTGTCATCTTCCTCCAAAAGGCTGATTTTATAGTCAACGAAGGGGGACATGTTTTTTATGaaa                                                     >  2:989249/1‑239 (MQ=255)                                                                                                                                                                           a‑aCGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATa                                                   >  2:332021/1‑239 (MQ=255)                                                                                                                                                                             aCGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:980520/1‑239 (MQ=255)                                                                                                                                                                             aCGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:412979/1‑239 (MQ=255)                                                                                                                                                                                   gATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa                                                                                                                     >  1:428107/1‑166 (MQ=255)                                                                                                                                                                                        ttgtttttGTGA‑‑AATGAC‑GTAGTTCAATTTTTTAAT‑TAAAAACAAAGGAGAAAAAATAAGCAAAAAAACTCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                        <  1:264654/235‑1 (MQ=255)                                                                                                                                                                                         acTTTTTGTGT‑‑AATTAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGAAGGAGTTAGAAGTGATAAACCAAGCCGAAAGCGACAATATCATCGGTAGAGATGCCATTGGGACCGTAGAAGCTGTCATCTTGATCCAACAGTTTAATTTTATCGTCAACGTAGGAGGCCATGTTTTTATTTAAAAAAAAAACCGCGCCa                                      >  2:648930/1‑239 (MQ=255)                                                                                                                                                                                                   t‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGt                            >  1:893449/1‑239 (MQ=255)                                                                                                                                                                                                       aTGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa                                                                                                                                                              >  1:99190/1‑107 (MQ=255)                                                                                                                                                                                                         agagggaaattacaTTTTTAATATAAAAAAAAAGGAGAAAAAAAAAAAAAAAACACGCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt                          <  2:256476/228‑1 (MQ=255)                                                                                                                                                                                                             agatacattttttttAAT‑TGAGAAAAAGGGAGAAAAAAAACAAAAAAAACCCCCGCCATAACAGCGGGAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc                     <  2:859023/229‑1 (MQ=255)                                                                                                                                                                                                              gAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCACAAGGTTGATTTTATCGTCAACGTAGTTGGCCATGTTTTTATTGAAATAGAAAGTTCCGCCAATATCGGGGGATTTaaac                    >  2:904960/1‑237 (MQ=255)                                                                                                                                                                                                                   cacaTTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCAACAGGGTGATTTTATAGTCAACGTAGGTGGACAGTTTTTTATTGAAAAAGAAAGTGGCGCCAAAATAGGCGAATTTAACCaaa                 >  2:1092095/1‑235 (MQ=255)                                                                                                                                                                                                                         tttAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:213943/1‑182 (MQ=255)                                                                                                                                                                                                                          taaaT‑TGAGATAAGAGGAAGAAGAAAAAACAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatca        <  1:412392/237‑1 (MQ=255)                                                                                                                                                                                                                                 tAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatctgtttct  >  1:859023/1‑232 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           GGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTTCATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTTGTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435485‑1435940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑