BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,027,731	A→G	13.0%	E204G (GAG→GGG)	ghxQ →	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,027,731	0	A	G	13.0%	272.6 / 3.8	111	E204G (GAG→GGG)	ghxQ	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand): ref base A (28/64); new base G (14/3); total (44/67)
Fisher's exact test for biased strand distribution p-value = 8.46e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATCTTCACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGG  >  NC_000913/3027496‑3027959

ttCTTCCCCTCCTTTTCCGTAACGGGTGGTCGTCCCGGGGTCTTACTAAATTTGCCGATGGGTATCGGTCACGGTACGGGTATCGGTATCGGGCTGTTTCTGG‑TGCTGATTGCTGCTAAC‑GGTGTGGGTAGGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGaa                                                                                                                                                                                                                                      <  2:792151/238‑1 (MQ=255)

     cACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGCGATGATGAGCTTGCTGGGGCTGGC‑GGCCATCTTCGGCCTTGGGAA‑gggt                                                                                                                                                                                                                                 >  1:477398/1‑238 (MQ=255)

               ttcggtcccGGTGTGCGTTCCTGGGTTTTTCGGATGTTGCCGGTGGGTATCGCCCACGGTAGAGGGATTGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCcgg                                                                                                                                                                                                                      <  2:684990/232‑1 (MQ=255)

               tCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCcgg                                                                                                                                                                                                                      <  1:651891/239‑1 (MQ=255)

                         gtgtGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGGCTGGGGAA‑GGGTCGGGTGACCgggggggggttg                                                                                                                                                                                                            >  2:145846/1‑233 (MQ=255)

                          tgtgCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GGGTCGCGGACCCGGCGGGACCtttt                                                                                                                                                                                                           >  1:521312/1‑237 (MQ=255)

                          tgtgCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGACTCTTCGGGCTGGAGAA‑GTGTGCCGTACCCGGCGGGCTCttgt                                                                                                                                                                                                           >  1:489275/1‑239 (MQ=255)

                            tgCGTACCTGGATCTTACGTAAGTTGCCGGTGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCttgttg                                                                                                                                                                                                         <  1:426865/239‑1 (MQ=255)

                                      gATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GGGTCGCGTACCCGGCGGGAGCTTGTTGGGGgttatt                                                                                                                                                                                                >  1:873422/1‑238 (MQ=255)

                                           tACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGCACCTTCGGCCTGCGGAA‑GGGTCCCGTACCCGGCGGGACCTTGTTGGGTGTTATTTCAAtt                                                                                                                                                                                          >  1:109108/1‑239 (MQ=255)

                                                  tttGGCGATGGGTATCGGTTGCGGTACCGGTTTCGGTATCGGGCTGTTCCTGC‑TGCTGATTGCTGCTAAC‑GGTTTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑Gatca                                                                                                                                                                                  <  1:501976/239‑1 (MQ=255)

                                                  tttGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTCGCGTACCCGACTGGAGCTTTTTGGGGATTATTTCAATTTT‑Gatca                                                                                                                                                                                  >  1:955434/1‑239 (MQ=255)

                                                  tttGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GGGTCGCGGACCCGGCGGGGTCTTGTTGGGGGTTATTGCAATTTC‑Gatca                                                                                                                                                                                  >  1:997212/1‑239 (MQ=255)

                                                   ttGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGGTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑Gatcat                                                                                                                                                                                 <  1:983107/239‑1 (MQ=255)

                                                        gttGGGTTTTGCTCACGGTACGGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCt                                                                                                                                                                            <  2:835332/237‑1 (MQ=255)

                                                               aTCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCtt                                                                                                                                                                     <  1:125406/239‑1 (MQ=255)

                                                                   cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCCTCTTCGGCCTGGAGAA‑GTGTCGCGGACCCGGCGGGATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGa                                                                                                                                                                  >  1:528558/1‑238 (MQ=255)

                                                                    ctacgggtcAGGTTGCGGTATTGGGGTTGTTCTGCGTGTTG‑TTGCTGCGAAC‑GGTGTGGGTAGGGTTAGCAAAAACCCGGTTGGAGGCTTGGCAGTGGG‑GCTCG‑GTGCGGTTACCTCCTTCCCGGTGATGAGGAGCTTGCTGGGGGTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGAcc                                                                                                                                                                <  2:1067640/231‑1 (MQ=255)

                                                                      aCGGTTCAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑G‑GTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑g                                                                                                                                                            <  1:282914/239‑1 (MQ=255)

                                                                        ggTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GTGGCGCGGACCCGGCGGGGACTTGTTGGTGTTTTTTTCTAATTT‑GGTCCCCGGGTTAATCTTTTACCCC‑Gc                                                                                                                                                           >  2:41765/1‑239 (MQ=255)

                                                                        ggTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGCGAA‑GTTGCGCGTCCCCGGCGGGGTCTTGTTGGTGGTTTTTGCAATTTA‑GCTCATTGGCTTAAACTTTGACCCA‑Gc                                                                                                                                                           >  2:273836/1‑239 (MQ=255)

                                                                        ggTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCACCCTCGGCCTGGCGAA‑GGGTCGCGGACCCGGCGGGGGCTTGTTGGGGTTTTTTGCAATTTC‑CTTCATCGGGTTAATCCTTGAaccccc                                                                                                                                                            >  2:1377/1‑236 (MQ=255)

                                                                         ggcagggtatcggtaacGGGCTGTTTCGGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAGCCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑g                                                                                                                                                            <  1:313442/232‑1 (MQ=255)

                                                                          tACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGGTTCTACC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGa                                                                                                                                                                                                                 <  2:834241/185‑1 (MQ=255)

                                                                          gaCAGGGATCGGTATCGGGCTGTTTCTGC‑TGCTTATGTCTGCTAAC‑GGTGTGGGTATGGTTAACAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑g                                                                                                                                                            <  1:755550/235‑1 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGTCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑CCGGGGAAATa                                                                                                                                                  >  1:102399/1‑238 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAAGGTGT‑GCGGACCCGGCGGGGTCTTGTTGGTGATTATTGCAAATTC‑GGTCATCGGCGTAATCTTTGACCCA‑GCGGGGaaaaa                                                                                                                                                  >  2:387102/1‑236 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTgcacta                                                                                                                                                  >  1:744601/1‑233 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAc                                                                                                                                                 >  1:497798/1‑239 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGCCTTAATCTTTGACCCC‑GCGGTGAAATa                                                                                                                                                  >  1:227631/1‑238 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAG‑GTGTCGCGTACCCGGCGGGATCTTGTTggg                                                                                                                                                                                                       >  1:834241/1‑184 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAcc                                                                                                                                                >  1:318946/1‑238 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCa                                                                                                                                               <  1:41765/239‑1 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTTCCCCA‑GCGGTGAAATAcc                                                                                                                                                >  1:639590/1‑238 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATTAGCTTGCTTGGGCTGGC‑GGTTACCTTCCGCCTGGGGAC‑GGGTCGCGTACCCCGCGGGCTCTTGTTGGTGATTATTGGAAGTTCGGATC‑TCGGTCTTATCTTTTACCCA‑AGGGGGAAATaaaccggtc                                                                                                                                          >  2:784210/1‑231 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GTGTCGCGTACCCGGCGGGGTGTTTTTGGTGGTTTTTGCAATTTG‑CATCATCGGCTTAATCTTTTACCCA‑GCGGGGaaaaaacatccat                                                                                                                                          >  2:313442/1‑229 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCAACTTCGGCCTGGGGAA‑GTGTCGCGTAACCGGGGGGATCCTGTTGGGGGTTATTGGAATTTC‑GATACTGGGGTTAATTTTTGACCCA‑CCGGGGGAAAACCCGGGTc                                                                                                                                          >  2:983608/1‑239 (MQ=255)

                                                                                            gCTGTTTCTGG‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCtg                                                                                                                                        <  1:638224/238‑1 (MQ=255)

                                                                                            gCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑Gatca                                                                                                                                                                                  <  1:501756/197‑1 (MQ=255)

                                                                                               gTTTCTGG‑TGGGGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCtggtgg                                                                                                                                    <  1:334674/239‑1 (MQ=255)

                                                                                                ttttTGT‑TGGTGTTTGTTGCTAAG‑GGTGTGGGTATGGTTATCAAAAACCCGATTGGAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGc                                                                                                                                   <  2:80064/239‑1 (MQ=255)

                                                                                                tttCTGC‑TGCTTTTTGCTTCTACC‑GGTGGGGGTATTGTTATCAAAAACCCTATTGAAGGCTTGCCAGGGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGc                                                                                                                                   <  2:884668/239‑1 (MQ=255)

                                                                                                tttCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGGTCTTGTTGGTGATTATTGCAATTTC‑GGTCAACGGCTTTAACTTTGTCCCC‑CCGGGTGAATACCCCGGTCcgggggg                                                                                                                                   >  2:871030/1‑238 (MQ=255)

                                                                                                               tttctttatc‑GGTGGGGTTGTGGTTATTAAAAACCCGGTGTAGGGCTGGGCAGTGGG‑GGTCT‑GGGCGTTTTCCTCCTTCCCGGGGATGCTGAGCTTGCTGGGGCTGGC‑GGTCAGCGTGGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGa                                                                                                                     <  2:334164/230‑1 (MQ=255)

                                                                                                                 gctctAACGGGTGT‑GGTTTGGTTTTCAAAAACCCGATTGGAGGGTTGGCAGTTGG‑GCTCG‑GGGCGGTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACt                                                                                                                   <  2:1046303/236‑1 (MQ=255)

                                                                                                                     tAAC‑GGTGTGGGTTTGGTTATCAAAACCCCGATTGAGGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                                               <  1:182394/239‑1 (MQ=255)

                                                                                                                      aaC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCCGGGGAA‑GGGTCGCGTACCCGGCGGGTTCTTGTTTGGGGTTATTGCAATTTC‑GGTCATCGGGCTAACTTTTGGACCA‑ACGGGGAAAAACCAACGTCTGGTGGCGATTCCAACCCTAACAgggca                                                                                                              >  2:255564/1‑236 (MQ=255)

                                                                                                                               gggTTTGGTTATCAAAACCCCGATTGAAGGCTTGCCAGTTGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTa                                                                                                      <  1:848347/239‑1 (MQ=255)

                                                                                                                               gggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGGTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:273836/238‑1 (MQ=255)

                                                                                                                               gggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGGGCTTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑TCGGTGACATACCACGACCTGGTGGCGATGCCAAGCCCGACTGGCGAAAATGGtc                                                                                                      >  1:720208/1‑238 (MQ=255)

                                                                                                                               gggTATGGTTAACAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCGTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:1377/238‑1 (MQ=255)

                                                                                                                                ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTa                                                                                                      <  1:871030/238‑1 (MQ=255)

                                                                                                                                    tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGCAATACCACGGTCTGGTGGCGATGGCAAGCCTGACTGGCGAAGATGGGAAggc                                                                                                  >  1:1037963/1‑236 (MQ=255)

                                                                                                                                       ttAGCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:869609/230‑1 (MQ=255)

                                                                                                                                          tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGGTCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGGAAGCCTCTGAtt                                                                                           >  1:203556/1‑239 (MQ=255)

                                                                                                                                          tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGCCTGGTGGCGATGCCAAGCCTGACTGTCGAAAATGGTAAGTCTCTGAtt                                                                                           >  1:796662/1‑239 (MQ=255)

                                                                                                                                          tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGAGCTTGTTGGTGGTTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCAGGCGGTGAAA‑ACCACGGGTCGGGAGCGGTGGCAAGGCCGACTGGCGGAGAAGGTaaaagcctggtt                                                                                           >  2:842421/1‑229 (MQ=255)

                                                                                                                                           cAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGCCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAttt                                                                                          >  1:130444/1‑239 (MQ=255)

                                                                                                                                            aaaaaCCCGATTGAAGGCTTGCCAGTGGC‑TGTCG‑GTGCGTTTGCCTCCTTCCCGGTGGTGATGAGGTTGCTGGGGCTGGC‑GGTCATCGTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAtttt                                                                                         <  1:35110/239‑1 (MQ=255)

                                                                                                                                                  ccGTTTTAAGGCTTTCCTGTGGC‑TCTCG‑GTTCGTTTACCTTCTTCCCGGTTATGATGAGGTTGCTGGGGCTGGC‑GGTCATCTGCGGCCTGGAGAA‑GTTTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCt                                                                                   <  2:541141/239‑1 (MQ=255)

                                                                                                                                                  ccGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTTCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCt                                                                                   <  1:765128/239‑1 (MQ=255)

                                                                                                                                                   cgttttaaGGCGTGCCAGTGGC‑GGCCG‑GGGCGTTTACCTCCTTCCAGGTGATGATGAGCTTGCTGGGGCTGGC‑GTTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:639590/233‑1 (MQ=255)

                                                                                                                                                   cgtttGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTTCCTCCGTCCCGGTGATTAGGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:203556/236‑1 (MQ=255)

                                                                                                                                                   cggttGGAGTCTTGCCGGGTGC‑GTTCG‑GGGCGTTTCCCCCCGTCCCGGTGATGAGTAGCTTGCTGGGGCTGGG‑GGTTAATTTCGGCCGTGAGAA‑GTGTCGCGTTCCCGGCGGGATCTTGGTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:477398/236‑1 (MQ=255)

                                                                                                                                                   cggttGAGGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTTCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:777803/236‑1 (MQ=255)

                                                                                                                                                   cggttGAAGGCTTGCCGGTGGC‑GGTGG‑GTGCGTTTACCTCCTCCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:444284/236‑1 (MQ=255)

                                                                                                                                                   cGATTGAATGTGTGCCAGGGGG‑GGTCT‑TGGCGTTTTCCTCTTTCCCGGTGATTATGGTGTTGCGGGGGCTGGC‑GGTCATCTTCGGCGTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTAATGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:183617/239‑1 (MQ=255)

                                                                                                                                                   cGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:983608/239‑1 (MQ=255)

                                                                                                                                                   cGATTGAAGCCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:884721/239‑1 (MQ=255)

                                                                                                                                                     aTTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTTGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGCAGATGGGAAGTCTCTGATTTTCACACTCGa                                                                                >  1:223353/1‑239 (MQ=255)

                                                                                                                                                     aTTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCCATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTTGTGGCGATGCCAAGCCTGACTGGCCAAGATGGTAAGGTTCTGATTTTCAGCCTCGa                                                                                >  1:239434/1‑239 (MQ=255)

                                                                                                                                                      ttGAAGGTTTGGCCGTTGC‑GCTCG‑GTGGGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGat                                                                               <  2:796695/239‑1 (MQ=255)

                                                                                                                                                          aGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtatta                                                                           <  1:598270/239‑1 (MQ=255)

                                                                                                                                                            gCTTGCCAGGGGC‑G‑TCGTGTGCGTTTTCCTCCTTCCCGGTGATTATGAGCTTGCGGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                                                          <  2:318946/238‑1 (MQ=255)

                                                                                                                                                             cTTGCCAGTGGCGGCTGG‑GT‑CGTTTCCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                                                          <  1:239771/237‑1 (MQ=255)

                                                                                                                                                                gCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGGTTATTGCAATTTC‑GATCATCGGCTTAATCTTTTACCCA‑GCGGGGAAATACCACGGGCTGGGGGCGATTCCCACGCTGACTGGCGCAGAGGGGAAGGTCCTGATTTTTACCCCCGATATTAAggggg                                                                     >  2:295415/1‑237 (MQ=255)

                                                                                                                                                                gCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                                               <  2:935334/197‑1 (MQ=255)

                                                                                                                                                                     gtgcggcTCG‑GTGCGTTTACCTCCTTCCCGGTGATGGGGAGGTTGGTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACt                                                                 <  2:873422/234‑1 (MQ=255)

                                                                                                                                                                         cgttCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCa                                                              <  1:387102/235‑1 (MQ=255)

                                                                                                                                                                           ttCG‑GTGGTTTTTCTTCTTTCCCGGTGATGTTGGGGTTGCTGGGGGTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGcc                                                           <  2:130444/238‑1 (MQ=255)

                                                                                                                                                                           cTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGGTCTTGTTGGTGATTATTGCAATTTC‑GGTCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAATTTCAGCCTCGATATTATGGGGGCAACCCAGcc                                                           >  1:42403/1‑239 (MQ=255)

                                                                                                                                                                           cTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGc                                                                                                                <  2:288514/186‑1 (MQ=255)

                                                                                                                                                                            tCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGcc                                                           <  1:672521/238‑1 (MQ=255)

                                                                                                                                                                             tgggggCGTTTGCCGTCTTCACGGGGAGGGTGAGGTGGCGGGGGTGGGA‑GGTCAGCTTCGGCCTGGAGAA‑GTGGAGCGGACCCGGCGGGATCTTGTTGGTGATTAGTTCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTTACTGGCGAAGATGGTAAGTCTCTGATTGTAAGCCTCGATATTATGGGTGCACGCCAGcc                                                           <  2:720208/233‑1 (MQ=255)

                                                                                                                                                                              tggggcttttACCTCCTTCCCGGTGATTGTGGGTTTGCTGGGGCTGGCGGGTCA‑CTTGGGCCTGGAGCA‑GTGGCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCAGCGTCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCaa                                                         <  2:109108/232‑1 (MQ=255)

                                                                                                                                                                                  gcctttCCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTTATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTg                                                      <  1:23464/235‑1 (MQ=255)

                                                                                                                                                                                        tccccctCCCCGGTGATGTGGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCGTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTcc                                               <  2:1054970/235‑1 (MQ=255)

                                                                                                                                                                                          ctcctTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                                             <  1:442061/239‑1 (MQ=255)

                                                                                                                                                                                              ttCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAgtgt                                         <  1:1038120/239‑1 (MQ=255)

                                                                                                                                                                                               tCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTTTGGGTGCACTCCAGCCAACTGTACTTCCCGgtgt                                         >  1:2453/1‑238 (MQ=255)

                                                                                                                                                                                                 ccGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTCCACTCCAGCCAACTGTACTTCCGAGTGTACt                                      <  1:145846/239‑1 (MQ=255)

                                                                                                                                                                                                      tatggtggGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCa                                  <  1:919090/237‑1 (MQ=255)

                                                                                                                                                                                                      ataagggactgtGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAt                                 <  1:570777/228‑1 (MQ=255)

                                                                                                                                                                                                          gttggtttgggggggGTGGC‑GGTTATTGTCGGCCTGGAGAA‑GGTTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATGCCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGt                             <  2:2453/228‑1 (MQ=255)

                                                                                                                                                                                                          gtgaGCTTGTCGGTGCTGGC‑TGTCATCTTCGGCCTGGGCAA‑GTGTCGCGTACCGGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GGTCATCGGCTTAATCTTTGCCCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGt                             <  2:919201/238‑1 (MQ=255)

                                                                                                                                                                                                               cTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAATCCGGCCCGGCGCAGGTGGGAAGTCTCTGCTTTTTACCCTCGATATTATAGGTGCACCCCCGCCCACTGTACTTCCGCGGGTACAgggcatgggggta                         >  2:156127/1‑227 (MQ=255)

                                                                                                                                                                                                               cTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAACACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTGTCTGATTTTTATCCCCGATATTTTGGGGGCACTCCAGCCAAATGTACTTCCCAGGGTACGGGCAATggggggga                        >  2:603670/1‑233 (MQ=255)

                                                                                                                                                                                                                               ggTCATCGTGGGCATGGAGAA‑GTGTCGCGTACCCGGTGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGAc          <  1:805148/238‑1 (MQ=255)

                                                                                                                                                                                                                                  cttttttggCCTGGGGAG‑GTGTGGCTTTCCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTa      <  2:1020548/232‑1 (MQ=255)

                                                                                                                                                                                                                                      ttCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTgg  <  1:1104516/239‑1 (MQ=255)

ATCTTCACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGG  >  NC_000913/3027496‑3027959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`