BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,817,477	T→C	9.6%	S268P (TCC→CCC)	yicC →	UPF0701 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,477	0	T	C	9.6%	256.3 / 2.1	102	S268P (TCC→CCC)	yicC	UPF0701 family protein
Reads supporting (aligned to +/- strand): ref base T (39/47); new base C (2/12); total (42/60)
Fisher's exact test for biased strand distribution p-value = 3.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGT  >  NC_000913/3817241‑3817715

tGGAAAATTCTCAGGTGGAACTGGAAAACCAGCGGCTGGAACAGGAAATGGTTCT‑GGTTGAAAA‑ACGAATTGCCGTTGCCGAACAACTGGAAGGCCTCGAAGCGCATGTCAAAGGGCACTAAAACAATCTGAAGAAAAAAGAAGCAGTTGGTCTTCGCCTGGATTTTATGATGAAGGAGCTCGACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTc                                                                                                                                                                                                                                               <  2:214753/238‑1 (MQ=255)

     gATGTTCCGGGGCAACTGGAAAACACCCGTTTGGGGCCGGAACTGGTTCT‑GCTGGCAAG‑ACGGATTGACGGTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGAACTGCAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGGGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGGCGAAGTGACAAACTCCGCCAt                                                                                                                                                                                                                                         <  2:571309/239‑1 (MQ=255)

       tGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGACTTGACGTTTCCGAAGAACTGGATCGCCTCGACGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAACCGGTTGGTCGTCGTCTGGCTTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATAAATGCCGAAAAGAAAAACTCCCCCAtag                                                                                                                                                                                                                                       >  2:786723/1‑237 (MQ=255)

         cTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAACAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGCAGCTTATCAATACCACAAGGACAAACCCCCCCaccaac                                                                                                                                                                                                                                     >  2:1019818/1‑234 (MQ=255)

         cTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCAGTTGGTCGTACTCTGGCTTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGCCGAAGTCTATCACTGCCGAAGTGACAAACCCCGCCATCggc                                                                                                                                                                                                                                     >  2:825090/1‑237 (MQ=255)

             gggtgcacgtgAAAACAAGCGGCTGGAGCTGGAACCGGGTCT‑GCTGGCACA‑ACGAATTGGCGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGTCCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGa                                                                                                                                                                                                                                 <  1:402011/229‑1 (MQ=255)

             ggggacaCTGGAAAGAAACCGGTTGGGGAAGGAACTGGTTTT‑GCTGGGCCA‑ACGAATTGGCGTTTCCGAAGAACTGGATCGGCTTGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGa                                                                                                                                                                                                                                 <  2:667980/233‑1 (MQ=255)

                 cAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTCTCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAACgt                                                                                                                                                                                                                             >  1:795442/1‑239 (MQ=255)

                  aaCTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAgt                                                                                                                                                                                                                             <  1:216211/238‑1 (MQ=255)

                   aCTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAACGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACCCCCTTGCGCCGAAGTCTCTCAATGCCGAAAGGAAAAACACCACCATCAAGCTGAAAgggt                                                                                                                                                                                                                           >  2:216211/1‑239 (MQ=255)

                      ggAAAACAACCTTCTGGGGCAGTAACTGGTTCT‑GCTGGCACA‑ACGAATGGACGGGTCCGAAGAACTGGATCGGCTCGAACGGACTGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttga                                                                                                                                                                                                                        <  2:709439/239‑1 (MQ=255)

                            caacCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCAAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGATCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCCCCAACGAGCTGAAAATGTTGAATGAga                                                                                                                                                                                                                  >  2:407219/1‑238 (MQ=255)

                                    tGGAGCAAGAA‑TGGGTCTCGCTGCCACA‑ATGAATTCACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGGCCGACGACATTCTGAAGAAAAAAGCAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTGAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTTTTGATTGAGCAGATgcg                                                                                                                                                                                                           <  1:554931/238‑1 (MQ=255)

                                    tGGAGAAGGAACTGGTTCT‑GCTGGCACACAGGAATAGACGTTGCCGAAGAACTGGATCGACTCGAAGCGCATGTCAAAGAGACCGACAACAGTCTGAAGAAAAAAGAAGCGGGTGGCCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATgcg                                                                                                                                                                                                           <  2:1068012/239‑1 (MQ=255)

                                        tcatggatgttttCT‑GGTGGCAAA‑ACGAATTGCTGTTGCCGAAGAAATGGATGGGCTCGAAGCGCATGTCAAAGGTCCCTTCAACATTCAGAAGAAAAAAGAAGCGGTTGGGCGTCGTCTGGATTATATGATGCGGGAATTCAACCGTGAGTCGAACACTCTTGCGTCGAAGTCGATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGc                                                                                                                                                                                                      <  2:903230/228‑1 (MQ=255)

                                         gtgggacggggtCT‑GCTGGCACA‑ACAAATTGGAGTTGCCGAAAAACGGGAACGCCTGGAAGCGAATGTCAAAGTGACCTACAACAGTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACGCTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGc                                                                                                                                                                                                      <  2:898533/227‑1 (MQ=255)

                                         cAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGc                                                                                                                                                                                                      <  1:871386/238‑1 (MQ=255)

                                         cAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGc                                                                                                                                                                                                      <  1:57851/238‑1 (MQ=255)

                                            gAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAAAACTCTTGCGTCGAAGTTTATCAATGCCGAAGGGCCAAACTCCGCCATCGACCTGAAAGGGTTGATTGACCAAATccgcagccaaaa                                                                                                                                                                                                  >  2:480899/1‑231 (MQ=255)

                                               tttgTTCT‑GCTGGCCCA‑ACGATTTTGCGATGCGCAAGTATTGGAACGCCTCGAAGCGCATGTCAAAGTGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGGCGGCGTCGGGATTTTATGATGCAGGAGTTCAACCGCGAGGCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCa                                                                                                                                                                                               <  2:234538/236‑1 (MQ=255)

                                                 ggTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGAGTTGATTGAGCAGATGCGCGAGCAGATTCAGa                                                                                                                                                                                             >  1:9535/1‑239 (MQ=255)

                                                   ttCT‑GTTGGCCCA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAATAGACCTACAACATGCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGaa                                                                                                                                                                                            <  2:813521/238‑1 (MQ=255)

                                                   ttCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCGCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGGCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGaa                                                                                                                                                                                            <  1:1031396/238‑1 (MQ=255)

                                                     ttttctgGGACA‑ACGATTTGCCGTTCGCGAAAAAATGGAGCGCCCGGAAGGTGATGTCAAGGATGCCTACAACATTCTGAAGAAAAAAGAAGCTGTTTGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAAc                                                                                                                                                                                           <  2:57344/234‑1 (MQ=255)

                                                          gtggcaa‑aCGAATTGACGTTGGCGAAGAACGGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGGAGCGGTTGGGTGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTGGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGaa                                                                                                                                                                                     <  2:279852/233‑1 (MQ=255)

                                                           ggCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGGGCTGAAAGGGTTGATTGGGCAGATGCGCGGGCAGATTCAAAACATCGAAt                                                                                                                                                                                    >  1:1091286/1‑239 (MQ=255)

                                                                   cgaagttgcGTTGGCGAAGAACTGGTGCGCCTCGGAGCGCATGTAAAAGAGACCTACAACATTCGGAAGAAAAAAGAAGGGGTTGGGCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTGGATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACACGt                                                                                                                                                                             <  2:767825/231‑1 (MQ=255)

                                                                        tgactgtGCCGAAGAACTGGATCGCCTCGAGACGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGc                                                                                                                                                                        <  1:15892/233‑1 (MQ=255)

                                                                           cGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCGGAACATCGAATAACTCGTAACGCCaa                                                                                                                                                                     >  1:237515/1‑239 (MQ=255)

                                                                                ccGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGttgatt                                                                                                                                                                                                                      >  1:217280/1‑185 (MQ=255)

                                                                                ccGAAGAACTGGATCGCCTAGAAGCGCATGTCAAAGAGGCCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGttgatt                                                                                                                                                                                                                      <  2:217280/185‑1 (MQ=255)

                                                                                 cGAAGAACTGGATCCCCTCCAAGCAGATGCTAAAGGAGCCTATCACATGTTGAATAAAAAAGAAGCCGTTGGTCGTCGTTTGGATTTTATGATGCCGGAGTTCAACCGCGAGTCGAACCCTCTTGCGGCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCtt                                                                                                                                                                <  2:504738/238‑1 (MQ=255)

                                                                                          ggATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGACGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGCAGATCCCGGAGCGGATTCAGAAAATCGAATAAACCGTAAAGCCAATTCTTACTTTTccc                                                                                                                                                       >  2:773765/1‑237 (MQ=255)

                                                                                                ccTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGGGCAGATGCGCGGGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTcac                                                                                                                                                >  1:473475/1‑239 (MQ=255)

                                                                                                ccTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGACCATCGAAAAACTCGTAAGGCCAATTCTTACTTTTCCGCCTTcac                                                                                                                                                >  1:490536/1‑239 (MQ=255)

                                                                                                ccTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACCCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCTGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGATAAACTCGCAACGCCAATTCTTACTTTTCCGCCttacc                                                                                                                                                >  1:268152/1‑236 (MQ=255)

                                                                                                         gcATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTTGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACCCTCTTGCGTCGAAGTCTAGCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTGCCGCCTTCACAAATgcc                                                                                                                                         <  1:518706/237‑1 (MQ=255)

                                                                                                                      aCCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGGCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACagag                                                                                                                          <  1:58636/239‑1 (MQ=255)

                                                                                                                       ccctaaacaTTCTGAAGAAAAAAGAACGGGTTGGCCGTCGTGTGGATTTTATGAGGCAGGAGTTCAACCGCGAGTGGAACCCTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCGGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACagag                                                                                                                          <  1:825770/233‑1 (MQ=255)

                                                                                                                       ccTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAATCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCCCCACTCAAACacagg                                                                                                                         >  1:154914/1‑238 (MQ=255)

                                                                                                                        cTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCCCCACTCaaaaaaaacg                                                                                                                        >  2:909551/1‑232 (MQ=255)

                                                                                                                        cTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAAAGCCCCCACTCAAACAAAGCg                                                                                                                        >  2:686347/1‑239 (MQ=255)

                                                                                                                          acaacaTTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCCCAAATGCCGCCACTCAAACAGACCg                                                                                                                        >  1:330075/1‑237 (MQ=255)

                                                                                                                                ttCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAAAGCCCATTCTTACTTTTCCGCCTTCACAAATTCCGCCACTCaacccaac                                                                                                                          >  1:972576/1‑223 (MQ=255)

                                                                                                                                ttCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAAAATCGAATAACTCTTAACGCCAATTCTTACTTTTCCGCCTTCAAAAATACCGCCCACCAAACAGAGCGGAAttttt                                                                                                                >  2:896648/1‑239 (MQ=255)

                                                                                                                                  cTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGt                                                                                                                                                                             <  2:936667/176‑1 (MQ=255)

                                                                                                                                  cTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGt                                                                                                                                                                             >  1:936667/1‑176 (MQ=255)

                                                                                                                                   tGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGGGTTGATTGAGCAGATGGTCGAGCAGATTCAGAACATCGAATAACTGGTAACGCCAATTCTTACTTTTCAGCCTTAACAAATGACGCCCCTCAAAAAAACCGGCAttttttt                                                                                                              >  2:216665/1‑236 (MQ=255)

                                                                                                                                         aaaaaGAGGTGTTTGGCCGTC‑TCGGGTTTTTATGTTGGAGGAGTTCAACCTGGAGTCGAACCCACTTGCTGCGAATTCTATAAAGCCGGAAGTGACAAACCCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACTCCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTCCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCa                                                                                                      <  2:451049/239‑1 (MQ=255)

                                                                                                                                          agaagaagAGGATGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGAGTTGATTGAGCAGATGCGCGGGCAGCTTCCGAACATCGAATAACTCTTACCGCCCATTCTTACTTTTCCGCCTTCCAAAATCCCGCCACTCAAAAAGAGCGGACTTTTTCTTCCCCGCa                                                                                                      >  2:133465/3‑239 (MQ=255)

                                                                                                                                               aagGGGTGGGTCGTCGTTTGGATTTATTGAGGCAGAGGTCCAACCGCCAGCCGACCCCTCTGGCGTAGAAGTATATCAAGGCCGAAGTGACAACCCCCGCCATCGGGCTGAAAGTGTTGATTGAGCAGAGGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCAAAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACAtt                                                                                                 <  2:752836/239‑1 (MQ=255)

                                                                                                                                                    gtggggcgcggtcTGGATTTTATGAGGCAGGAGTTCAACCGCGAGTCGAACCCTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAAt                                                                                             <  1:686347/228‑1 (MQ=255)

                                                                                                                                                       ggttgcgttttGGATTTTTGGAGGCAGGGGTTCACCCGGGGGTGAAACCCTCTGTGGTCGAAGATTATCAATGCCGAAGTGACAACCTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGGGAGCAGTTTCAGAACATCGATTACCTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTg                                                                                         <  2:795442/229‑1 (MQ=255)

                                                                                                                                                       gggcgtcgttTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACCCTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTg                                                                                         <  1:179384/236‑1 (MQ=255)

                                                                                                                                                                   gATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTTTTTTGCggc                                                                                >  2:934353/1‑234 (MQ=255)

                                                                                                                                                                         atgatgCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGccccc                                                                       >  1:460460/1‑238 (MQ=255)

                                                                                                                                                                                 ggAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAAAGCGGCATTTTTCTTCCCCCCAACATTCAATTCTGTTTTTCGTGCCTGCTCCAGATTTTGc                                                               >  2:725238/1‑239 (MQ=255)

                                                                                                                                                                                       cAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCTTGCCCGCCCCCGATTTTGCGATGtt                                                         >  2:721507/1‑239 (MQ=255)

                                                                                                                                                                                       cAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTTCGATGtt                                                         >  1:437620/1‑239 (MQ=255)

                                                                                                                                                                                       cAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGACGtt                                                         >  1:88160/1‑239 (MQ=255)

                                                                                                                                                                                            gcgAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCCGATTTTGCGATGtttttt                                                     >  1:239016/1‑238 (MQ=255)

                                                                                                                                                                                            gcgAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCCCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGttttttt                                                    >  2:111703/1‑239 (MQ=255)

                                                                                                                                                                                               aGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCCCTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTACATTCTGTTTTGCGTGCCTGCTCCCGCTTTTGCGATGTTTTTTTGc                                                  >  2:149137/1‑238 (MQ=255)

                                                                                                                                                                                               aGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGcc                                                 >  2:518080/1‑239 (MQ=255)

                                                                                                                                                                                                 tCGAACACTCTTGGGTCGAATGCTATCAATGCCGAAGTGAAAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGccc                                                <  1:243528/238‑1 (MQ=255)

                                                                                                                                                                                                 tCGAACACTCTTGCGTCGAAGTCTAACAATGCCGAAGGTACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAAGGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGccc                                                <  1:133465/238‑1 (MQ=255)

                                                                                                                                                                                                    aaCGCTTTTGGGGCGGAGTTTATCAATGCCGAAGTGAACACCCCCGCAACCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACCGGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAg                                              <  2:900114/237‑1 (MQ=255)

                                                                                                                                                                                                           ttgtgtgaaaGTTTAGCAATGCCAAAGTGAAAAACCCCCCCAACGAGCTGAAAGTGTTGATTGAGCAGATTCGCGAGCAAATTCAGAAACACAAAAAACTCGTAACGCCAATTTTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTgag                                     <  2:1091286/231‑1 (MQ=255)

                                                                                                                                                                                                           ttGCGTCGAAGTATATCACTGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGCTTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCTGCCTTCACAAATGCCGCCACTCAAACAGCGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGAGCCTGCTCCAGTTTTTGCGATGTTTTTTTGCCCCGCCCACTtag                                     >  1:115974/1‑239 (MQ=255)

                                                                                                                                                                                                                   tattcatgtaaTGCCGAATTGACAAACTCTGCAACCGAGCTGAAAGTGTTGATTGAGCAGCTGCGCGGTCAGATTCAGAACATCGAAAAACTCGTAACGCAAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             <  2:220396/230‑1 (MQ=255)

                                                                                                                                                                                                                   atgTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCAACTCAACCAGAGCGGCATTTTTCTTCCCCTCAACATTCAATTCTGTTTTGCGTGCCTACTCCAGCTTTTGCGATGTTTTTTTGACCAGCACACTGGGAACGagag                             >  2:554059/3‑239 (MQ=255)

                                                                                                                                                                                                                   aattCTAACAATGCCGAAGTGAAAAACTCCGCCATCGAGCTAAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             <  1:773765/236‑1 (MQ=255)

                                                                                                                                                                                                                   aaGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTTAGCAGCTGCGCGAGCAGATTCCGCCCATCGAATCCCTCGTCCCGCCACTTCTTCCTTTTCCGCCTTCCACCATGACGCCCCTAAAACAGCGCGGCCTTTTTCTTCCCCGCAACATTACCTTCTGTTTTGCGTCCCTGCTCACGCTTTTGCGCAGTTTTTTTGCCCAAAACACTgccacagtggg                             >  2:765812/1‑229 (MQ=255)

                                                                                                                                                                                                                   aaGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTgaga                                    >  1:900114/1‑232 (MQ=255)

                                                                                                                                                                                                                   aaGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             >  1:533857/1‑239 (MQ=255)

                                                                                                                                                                                                                    atgttctaaaagCCGAAGGGACAAACACCGCAATCGATCAGAAACTGTTGATTGAGCAGTGGCGGGACACAATTCAAACAAGAGAATCACCCGAAACGCCATTTCGTACTTTCCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGGTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGGgag                             <  2:237515/227‑1 (MQ=255)

                                                                                                                                                                                                                    aGTTTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             <  1:407219/238‑1 (MQ=255)

                                                                                                                                                                                                                    aGTCTACCAAGGCCGAAGGGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             <  1:721507/238‑1 (MQ=255)

                                                                                                                                                                                                                    aGTCTAACAAGGCCGAAGTGACAAACTCCGACTTCGAGCTGAAAGTGTTGATTGAGCAAATGCGCGAGCAGATTCAGAACATCGAATAACCCGTAACGCCAATTCTTACTTTGCCGCCTACACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTgag                             <  1:434577/238‑1 (MQ=255)

                                                                                                                                                                                                                       cTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATa                          >  1:44575/1‑238 (MQ=255)

                                                                                                                                                                                                                       cTAACAATGCCGAAGTTAAAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGAt                           <  1:46015/237‑1 (MQ=255)

                                                                                                                                                                                                                        tataAAGGCCGAAGGTAAAAGCCCCGCCAATGAGCTGAAAGTTTTGATTGAGCATATGAGCGAGCAGATCCAGAACATGAAATAAGCCCAAACGCCAATCCTGCCTTTTCCGCCTCCAAAAATGCCGCCACTCAAACAGGGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACt                        <  2:115974/239‑1 (MQ=255)

                                                                                                                                                                                                                        tATCAATGCCGAAGTGAAAAACCCCGCCATGGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACt                        <  1:786723/239‑1 (MQ=255)

                                                                                                                                                                                                                         aTCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGACCACTGAGAACGTGGGATACTc                       >  1:220396/1‑239 (MQ=255)

                                                                                                                                                                                                                         aTCAATGCCGAAGTGACAAACCCCGCCAGCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTc                       <  1:149137/239‑1 (MQ=255)

                                                                                                                                                                                                                          tCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                      <  1:934353/239‑1 (MQ=255)

                                                                                                                                                                                                                          tCAATGCCGAAGTGAAAAACCCCGCCAGCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTca                      <  1:480899/239‑1 (MQ=255)

                                                                                                                                                                                                                           cagtGCCGAAGTGCCAACCCCCCCCTTCGAGCTGAAATTTTTGATTGAGCAGATGCGGGAGCATATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                     <  2:490536/236‑1 (MQ=255)

                                                                                                                                                                                                                           cAAGGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                     <  1:875637/239‑1 (MQ=255)

                                                                                                                                                                                                                           aaaTCCCGAATTGACAAACTCCGCCATCGAGTTGAAAGTGTTGATTGAGCAGAGGCGCGAGCAGATTAAGAACAGCGAATAACCCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                     <  1:765812/238‑1 (MQ=255)

                                                                                                                                                                                                                             aaggggcaGTGAAAAACTCCGCAATCGAGCTGAAAGGGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACaa                   <  1:350293/232‑1 (MQ=255)

                                                                                                                                                                                                                              tGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAgt                                                                                                                                                                                                                             <  1:609770/36‑1 (MQ=255)

                                                                                                                                                                                                                              agacagggTGACAAACTCCGCTACAGAGCTAAAAGTGTTGATGGAGCAGATGCGCGGGCGAATTCAAAAAAACGAATAACTCGTAACGCCAATTTTTACTTTCCCCCCTTAACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAAc                  <  2:686112/232‑1 (MQ=255)

                                                                                                                                                                                                                                ccGAAGTGACAAACTCCGCAATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                <  1:843476/239‑1 (MQ=255)

                                                                                                                                                                                                                                ccGAAGTAACAAACCCCCCTATCGAGCTAAAAGTTTTGGTGGAGAAGATGCGCGAGCAGATTCAGAACAACAAATAACCCGTAACCCCAATTCTTATTTTTCCGCCTTCAAAAATGCCGCCCCTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTTAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                <  2:578765/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cGAATTGACAACTCCCGCCATCGGGCTGAAAGTTTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCAAATAACTCGTAACGCCAATTCTTACTTTGCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                <  1:821357/238‑1 (MQ=255)

                                                                                                                                                                                                                                  gAAGTGACAAACTCCGCCATCGAGCTTAAAGTTTTGATTGAGCAGATGCCCGACCAGATTCAGAACATCAAATAACGCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGta              <  1:909551/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gAAGGGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACCCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGta              <  1:896648/239‑1 (MQ=255)

                                                                                                                                                                                                                                        aCAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAg                                                                                                                                                                                                                   >  2:609770/1‑36 (MQ=255)

                                                                                                                                                                                                                                              tCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTTCTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGt  <  1:216665/239‑1 (MQ=255)

TGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCT‑GCTGGCACA‑ACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGT  >  NC_000913/3817241‑3817715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`