Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,098,366	C→A	15.4%	Q211K (CAA→AAA)	rhaS →	transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,098,366	0	C	A	15.4%	146.3 / 2.6	82	Q211K (CAA→AAA)	rhaS	transcriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base C (49/19);  new base A (3/11);  total (52/30)
Fisher's exact test for biased strand distribution p-value = 6.04e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

AGGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACG  >  NC_000913/4098136‑4098599                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑TCGTACGCTACATCGGCAGGTTAAACA‑GCCAACggg                                                                                                                                                                                                                                       >  1:669472/1‑238 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACggg                                                                                                                                                                                                                                       >  1:596579/1‑238 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACggg                                                                                                                                                                                                                                       >  1:424728/1‑238 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑ACGTACGCTAAATCGGCAGCTTAAGCA‑GCAAACGGGa                                                                                                                                                                                                                                      >  1:181268/1‑239 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGCCGGATCAA‑TTTT‑CTTTTTCATT‑CCGTCAGCTACATCGGCAGCTTAAGCA‑GCaaaacgg                                                                                                                                                                                                                                       >  1:436105/1‑234 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGCCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTCCATCGGCAGCTTAAGCA‑GCAAACggg                                                                                                                                                                                                                                       >  1:1003123/1‑238 (MQ=255) agGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TAAGGTGAATTGGGATGCCGTGGCGGACCAA‑TTTT‑TCCTTTCACT‑GGGTACGCTACATCGCCGGATTAAGCACGAAAACgg                                                                                                                                                                                                                                        >  1:78641/1‑238 (MQ=255)   gAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACAATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGGTGAC‑TTTT‑ATCTTTCCCT‑GCGGAAGCCACATCTGCAGGCTAAAAA‑GCaaaacggaac                                                                                                                                                                                                                                    >  2:26291/1‑232 (MQ=255)     aGGGGAAAATTTTTTCCCCCTGACCCGCCAGTCGGGAGATCTTGTTTATGCAAGTACTGCTCTTGCTGCGTAAAAGCAGGTTGCAGGGGAACCTGGAAAACAGCGCATCGC‑GTCTCAACTTGCTTGTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACt                                                                                                                                                                                                                                    <  2:1079356/238‑1 (MQ=255)     aGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGTTCAA‑TTTT‑TTCTTTCACT‑GCGTACGCTAACTGGGGCGGTTAAGGA‑GCCAACGGGACTg                                                                                                                                                                                                                                   >  1:344945/1‑238 (MQ=255)     aGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGGTCAATTTTT‑TTCTTTCA‑T‑GGGGACGCTAAATCGGGACGTTAAAGA‑GCAAAAGGGAcat                                                                                                                                                                                                                                   >  2:983625/1‑236 (MQ=255)      gggaAAAATTTTTTCCCCCCGC‑CCGGCAAGCGGCAGGTCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑a                                                                                                                                                                                                                                 <  2:291179/238‑1 (MQ=255)         gAACATGATTTTCCCCGTA‑CCCTCAGTCGGGAGGTCTTGGTTAAGCAAGTACTGCTCTTGCTGCGTAAAAGCTGGTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGc                                                                                                                                                                                                                              <  2:702186/238‑1 (MQ=255)         gAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGGGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTAAGCACCATCGGCGACTTAAACA‑GCAAACGGGGCTG‑Acac                                                                                                                                                                                                                              >  1:741395/1‑236 (MQ=255)          aaaaTGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGGGGGGAATTGGGTGGCCGTGGCGGGTCAA‑TTTT‑CTCTTTCACT‑GCGGACGCTACAACGGCACCTTATGCA‑GAAAAACGGGCTG‑Acccc                                                                                                                                                                                                                             >  2:1071407/1‑238 (MQ=255)                 tttACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGCGAATTGGGATGCCGTGGCGGATAAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAACCCGGACTG‑AGGCCTCACCGAt                                                                                                                                                                                                                     >  1:13703/1‑239 (MQ=255)                         cGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑TCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑AAGCCCCAAGCATACCCGaa                                                                                                                                                                                                              >  1:1103365/1‑238 (MQ=255)                          gA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCCCT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAAc                                                                                                                                                                                                             >  1:116917/1‑238 (MQ=255)                          gA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGGCTG‑ACGCCTCAGCGATACCTGAAc                                                                                                                                                                                                             >  1:802548/1‑238 (MQ=255)                                 cAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTT‑ACGCCTCAGCGATACCTGAAccgcccgc                                                                                                                                                                                                      >  1:952824/1‑239 (MQ=255)                                                   taggaaaTTCCTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAACACGCGCATCCC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGGGGCGGATCAA‑TTTT‑CTCTTTCACG‑GCGTACGCTACATCGGCAGCTTAAGCA‑GAAAACGGGACTG‑ACGCCTCAGCGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGAc                                                                                                                                                                                   <  1:950670/234‑1 (MQ=255)                                                     tGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGATGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTAAGCTACATCGGCAGCTTAAGCA‑GCAAAAGAGACTG‑ACGCCTCAGCGATACCCGAACCCCCCTGGCGCG‑ATGAAAGccccgaac                                                                                                                                                                                 >  2:81208/1‑234 (MQ=255)                                                          ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGCCATCTGCt                                                                                                                                                                            >  1:740075/1‑239 (MQ=255)                                                          ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCt                                                                                                                                                                            >  1:121275/1‑239 (MQ=255)                                                               gCTTTTGGTGTGTAAACGCAGTTTGGAGGAGACCCTGGAAAACAGCCCTTCAC‑GTCTCAAGGTGCTGGTGGCCTGGTGGGAGGACCATTGTGCGGA‑GGGGGTGAATGGGGTTGCGGGGGCGGATCAT‑TTTT‑CTCTTCCACTCGCG‑ACCCTACAGCGGCAGCTTAAGCA‑CAAACCGGGCCTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGcc                                                                                                                                                                       <  2:596579/239‑1 (MQ=255)                                                                  ctTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAACCGGGACTG‑ACGCCTCAGCGATACCTTAACCCCCTGCGACTG‑ATGAAAGCCCGCCAACTGCTACGcccc                                                                                                                                                                     >  1:319770/1‑236 (MQ=255)                                                                  ctTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCAAT‑GCGTACGCTACATCGGCAGCTTTAGCA‑GCAAACGGGACTA‑ACGCCTCAGCGATACCTGAACCGCCTCCGACTG‑ATGAAAGCCCGAAATCATCTACGcacaa                                                                                                                                                                    >  1:273771/1‑235 (MQ=255)                                                                   tggtggcGAAAAAGCAGTTTGCGGGAAACCCGGGAAAACCGCGCATACG‑GTCTCAACTGGCTTCTGGCCGGGCGGGAGGCCCTTTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGACCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAg                                                                                                                                                                  <  1:1071407/234‑1 (MQ=255)                                                                     gctgcGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCCAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCCGCGACTG‑CTGAAAGCCCGACATCTGCTTCGCC‑ACAGCg                                                                                                                                                                >  1:666998/1‑239 (MQ=255)                                                                     acggcGTAAAAGCGTTTTGAAGGAGAACCGGGAAAACAGCGCATCCC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCTTTTTGCCGA‑TGAGGAGAATGGGGATGCCGTGGCGGATAAA‑TTTC‑CTCTTTCACT‑GCGTACGCTACATGGGAGGCTTAAGAA‑GAAAAGGGGACGG‑ACGCCTCGGCTATACCTAAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGc                                                                                                                                                                 <  1:947407/235‑1 (MQ=255)                                                                        gggTAAAAGCAGTTTGAAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTGGCTTCTGGCCGGGCTGGAGGACCATTTGGCCGA‑TGAGGTGAATTGGGATGCCTTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTCCGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAg                                                                                                                                                              <  1:607668/236‑1 (MQ=255)                                                                           tAAAAGCTGTTTGCAGGATAACCT‑GCAAACCGCCCACCCCGGTGTCAATTTGTTTCTGGCCTGGCTGGAGGCCCATTTTCCCGA‑GAGGGGGAATTGGGATGCCGTGGCGGTTCAA‑TTTT‑CTCTTTCCCT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCCGCGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCa                                                                                                                                                          <  2:802548/239‑1 (MQ=255)                                                                           tAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACTGGACTG‑ACGCCTCAGCGATACCTGCAACGCCTGCGCCTG‑ATGAAAGCCCGACATATGCTACGCC‑CCAGCGAGGcc                                                                                                                                                           >  1:495206/1‑238 (MQ=255)                                                                           tAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCCCACGGTTACCTGGACCGCCCGCGACCGAATAAAAGCCC‑ACAACCGCTAACCC‑ACAGCGAGGCCa                                                                                                                                                          >  2:960364/1‑239 (MQ=255)                                                                           tAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑ACAAACGGGTCTG‑ACGCCCCAGCGGAACCTGCACCCCCCGCGACCG‑TAGAAAACCCCCCATCTGATCCCAC‑ACAACCAGGcc                                                                                                                                                           >  2:624809/1‑238 (MQ=255)                                                                           gaaagggggTTTGGAGGAAACCCGGGAAAACAGGGCACCGC‑GTCTCAACTTGCTTTGGGCCGGGCTGGAGGACCTTTTTGCCGG‑TGAGGGGAATTGGGATGCGGGGGCGGATAAA‑TTTT‑CTCTTCCACT‑GCGTCCGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCa                                                                                                                                                          <  2:121275/231‑1 (MQ=255)                                                                            aaaaGCAGTTTGCAGGAGAACCGGGAAACCGGGCCATCCC‑TGCTAATCTTGTTTCGGGCCTGGCTGGAGGACCATTTTGCCGA‑GGAGGTGAATTGGGATGCCGTGGCGGATAAA‑TTTT‑CTCTTTCATT‑GCGTACGCTACAGCGGCAGCTTAAGCA‑GCAACCGGGACTG‑ACGCCTAAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCa                                                                                                                                                          <  1:955855/238‑1 (MQ=255)                                                                                    tttGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCCGCCCCTTCAGCA‑CACAACGGGGCTG‑ACGCCTCACGGATACCTCAACCCCCTGCGCCTG‑ATTTAAACCCGCCACACGCGACCCC‑CCACCGAGGcaacaccctg                                                                                                                                                   >  2:947407/1‑228 (MQ=255)                                                                                     ttGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAACCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCggtcatg                                                                                                                                                 >  1:758043/1‑232 (MQ=255)                                                                                      tGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGC‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATAGGCCGCTTAAGCA‑GCAAACGGGACTG‑CCGCCTCAGCGATACCTTAACCGCCCGCGACGG‑ATGAAAACCCCACAACTCCTACCCC‑CCAACAAGGCCaaagggacgaaa                                                                                                                                               >  2:967328/1‑228 (MQ=255)                                                                                      tGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGAc                                                                                                                                               >  1:871527/1‑239 (MQ=255)                                                                                       cggtggaacccTGAAAAACTGCCCATCCC‑GTCTCACCTTGTTTGTGGCCTGGTTGGAGGCCCATTTTCCCGA‑TGAGGTGAATTGGTATGCCGGGGCGGATAAA‑TTTT‑CTTTTCCACT‑GCGTACGCTACAGCGGCAGCTTAAGCA‑GAAAACGGAACGG‑ACCCCTCAGCGATCCCGGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACa                                                                                                                                              <  2:1003123/230‑1 (MQ=255)                                                                                                ccTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTTAACCGCCTGCGGATG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGAAATCGCCTAt                                                                                                                                      >  1:540603/1‑238 (MQ=255)                                                                                                                       ctcAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCCCCTTTCGCTGTGGATTCAGCGACAGTa                                                                                                                >  1:9743/1‑238 (MQ=255)                                                                                                                        caaccTTGCTTCGGGCCTGGCTGGAGGACCATTTTGCCGA‑TGGGGTGAATGGGGATGCCGGGGGGGAACAA‑TTTT‑CTTTTGCCCT‑GCGTACGCTACATCGGCAGCTAAAGCA‑GCAAACGGGACTG‑ACGCCTAAGCGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAAc                                                                                                              <  1:188838/235‑1 (MQ=255)                                                                                                                          aaCTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCGCCTACGCC‑ACACCGAGGCCAGCGTTACTGAAATAGCCTTTCGCTTGGGATTAACCGACGGTAAcc                                                                                                             >  1:59624/1‑238 (MQ=255)                                                                                                                               gCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑TTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGCCATCTGCTACGCC‑ACAGCCAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCCACAGGAAACACctt                                                                                                        >  1:39589/1‑238 (MQ=255)                                                                                                                               gCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGGACGCTACATCGGCAGCTTAAGCC‑GCCAACGGGACTG‑CCGCCCCAGCGATACCTTAAACGCCCGCGGCTG‑ATGGAAGCCCGACATCTGCTCCGCC‑CCAACGAGGCCCGCGGTACTGGCACTCCCTATCGCTGTGGGTTCAGCGCCCACAACCACcttt                                                                                                       >  2:661505/1‑239 (MQ=255)                                                                                                                                 ttCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATAGCTGTGGCTTCAGCGACAGAAACACCTTTTc                                                                                                      >  1:162480/1‑238 (MQ=255)                                                                                                                                          ggctggAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAACGACAGGAACCCCTTTTCGACGCcttt                                                                                             >  1:444536/1‑238 (MQ=255)                                                                                                                                           gctggAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTTAGCA‑GCCAACGGGACTG‑ACGCCTCCGCGATACCTGAAACGCCTGCGACTG‑ATGAAAACCCGACATCTGCCACGCCAACACCG‑GGCCCCGGTTACTGACATCTCCCACCCCCGTGGATTTACGGACACAAAACCCTCTTCGACCCctttt                                                                                            >  2:4895/1‑238 (MQ=255)                                                                                                                                              ggaggaCCATTTTGCCGA‑TGAGGTGAAGTGGGATGCCGTGGCGTATCAA‑TTTT‑CTCTTTCACT‑GCGTCCGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑CCGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTCCGcc                                                                                        <  1:502509/239‑1 (MQ=255)                                                                                                                                               gtggaCCCTTTTGCCCAGTGGGGGGAATTGGGTGGCCGTGG‑GGAACAA‑TTTTCCTTTTCCCCT‑GCGGCCGTAACATGGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCGCGGCGATCCCGGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGcc                                                                                        <  2:669472/237‑1 (MQ=255)                                                                                                                                               ggggacattttgGCAGA‑GGAGGTGAATGGGGATGCGTTGGCGGTTCAA‑TTTT‑CTCTTCCACT‑GGTTACGCTACTCAGGCAGATAAAGCA‑GCAAAGGGGAATG‑ACGCCTGAGCGATACATGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGcc                                                                                        <  1:592690/230‑1 (MQ=255)                                                                                                                                               ggagaCCATTTTGCCGA‑TGGGGGAAATTGGGTTGCCGTGGCGAATAAA‑TTTT‑CTCTTCCCCT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACGG‑CCGCCCCAGCGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGcc                                                                                        <  1:7439/235‑1 (MQ=255)                                                                                                                                                aggaCCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTACCCACTTTTCGACGCtttttgcccta                                                                                      >  1:532805/1‑233 (MQ=255)                                                                                                                                                 ggaCCATTTTGCCGA‑TGGGGTGAATGGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACG‑GCGTACGCTACATCGGCAGCTTAAGCA‑GAAAACGGGACTG‑ACGCCTCAGCGATCCCGGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCga                                                                                      <  1:533433/238‑1 (MQ=255)                                                                                                                                                     cATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑TTCTTTCACT‑GCGTACGCTACATCTGCAGCTTAAGCA‑GCAAACGGGGCTT‑GCGCCTCAGCGATACCTGAACCGCCTCCGCCTG‑CTGAAACCCCGAACTCTCCTACGCC‑ACCACCGGGCCACGGGTCCTTCCCTCCCCAATCGCCGGGGATTCTGCCACCAGAACCACCTTTCAACGCCTTTTCCCCAAGAGtt                                                                                 >  2:975932/1‑239 (MQ=255)                                                                                                                                                               a‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCCCCGGCAGTTTAACTGGTc                                                                        >  1:1082803/1‑238 (MQ=255)                                                                                                                                                                          ttGGGCTGCTGTGGGTGTCCAT‑TTTT‑CTCTTCCCCT‑GCGTGCGCTACCTCGGCAGTTAAAGAA‑GCAAACGGGACTG‑ACGCCTCGGGTACACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGTTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGata                                                             <  2:424728/239‑1 (MQ=255)                                                                                                                                                                            gggtggcgggggCGCATAAT‑TTTT‑CTCTTTCCCT‑GCGTCCGCAACAGCGCCACTTCAACCA‑AAAAAGGGGACGG‑ACACCTCAGCGATACCTGCACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATAtt                                                           <  2:758043/229‑1 (MQ=255)                                                                                                                                                                                 gCCGGGGCGGAACAA‑TTTT‑CCTTTTCACT‑GCGTCCGTCACACCGGCAGTTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATCCCTGAGCCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGcc                                                       <  1:661505/238‑1 (MQ=255)                                                                                                                                                                                      ggtggATAAA‑TTTC‑CCTTTCCAGT‑GCTTACGGTACATCGGCAGCTTAAGCA‑GCAAACGGGAGTG‑CCGCCTCAGGGTTCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGa                                                  <  1:579385/235‑1 (MQ=255)                                                                                                                                                                                      ggcggtatacttttt‑ttttttCTCT‑GCGTACCGTACATCGGAATCTGAACCC‑GCAAAC‑GGTCTGCACCCCCCGGGGATCCCGAAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGa                                                  <  2:540603/228‑1 (MQ=255)                                                                                                                                                                                       gCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACg                                                >  1:595482/1‑239 (MQ=255)                                                                                                                                                                                               a‑TTGT‑CTTTTACACC‑GCGTACGCTAAAACGGCAGTTTAATCA‑GAAAACGGGTCTG‑GCGCCACAGCGATACCGTAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCt                                        <  1:321097/239‑1 (MQ=255)                                                                                                                                                                                                                    tACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAt                      >  1:162758/1‑239 (MQ=255)                                                                                                                                                                                                                      cGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGTGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtct                    >  1:897158/1‑239 (MQ=255)                                                                                                                                                                                                                        cTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑CCAGCGAGGCCATCGTTACTGACATCCACTATCGCTGTGGATTCAGCGACCGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAAATGGTCACCGCGTGCTATTGGCCAGGGACGGGGATGCTTTTTGCAAGAACCCGGAactt                   >  2:712304/1‑238 (MQ=255)                                                                                                                                                                                                                         taatgcGGCATCTTAAGCG‑GCAAACGGGACAG‑CCCCCACAGCGATACCTAAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttct                 <  1:624809/234‑1 (MQ=255)                                                                                                                                                                                                                            ctCGGCAGCTTAAGCA‑GAAAACGGGATGC‑GCGCCACCGTGATCCCAAAACCGCCTGCGACTG‑ATGAAAGCCCGACATCGGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa              <  1:130831/238‑1 (MQ=255)                                                                                                                                                                                                                            aTCGGCAGCTTAAGCC‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACCTCAGCTACGCC‑ACACCGAGGCCAGCGTTACTGACATCGCCAATCGCTGGCGATTCAACGACAGGTCCCACTTTTCCACGCTTTTTCTCCACGAGTTTCACGGGTCACCGCTGGCAATTCCCCAGGGACGGGCTCGATTTCTCAACTACCGCGaaattcctca               >  2:1094113/1‑230 (MQ=255)                                                                                                                                                                                                                            aTCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa               >  1:634829/1‑238 (MQ=255)                                                                                                                                                                                                                            aTCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGAAATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa               >  1:782324/1‑238 (MQ=255)                                                                                                                                                                                                                              cGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGGGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg            >  2:836697/1‑239 (MQ=255)                                                                                                                                                                                                                               gcacgCTTAAGCA‑GCAACCGGGACTG‑CCCCCGAGGGGATGCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGGCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt           <  2:444536/235‑1 (MQ=255)                                                                                                                                                                                                                                 cAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGCGGGTTTAACTGGTCCCCGCGTGCTATTAGCCAGGGACGGGAGGGCTTTGTGCAATAACGCGCATCTTCTCCACGTAt         >  2:130831/1‑239 (MQ=255)                                                                                                                                                                                                                                 cAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCCCTGTGGATTCAGCCACAGTAACCACTTTTCGAGCCTTTTTCGCCCAGAGTTTAACTGGTACCCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCATTAACCCGAAATTTCTCAACGTAt         >  2:1088309/1‑239 (MQ=255)                                                                                                                                                                                                                                  actttAAGCA‑GACAAGGGGCATG‑ACGCCTCAGCGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAt         <  1:1036703/235‑1 (MQ=255)                                                                                                                                                                                                                                  actttAAGCA‑GAAAACGGGACTG‑CCCCCTCGGCGTTCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAt         <  1:4895/235‑1 (MQ=255)                                                                                                                                                                                                                                   gCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGCGTTTAACTGTTCACCGCGGGATATTCCCCAGGGACGGGCTGGCTTTTTGCAATAACTCGAATCTTCCCAACGTAttt       >  2:236917/1‑239 (MQ=255)                                                                                                                                                                                                                                      tAAGCA‑GAAACGGGGACTG‑ACGCCCCAGTGATCCCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTa    <  1:960364/239‑1 (MQ=255)                                                                                                                                                                                                                                        agca‑gaAAGCGGTACTC‑GCGCCTAGGCTACCCCTAACCCGCCTTCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTTTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg  <  1:286107/239‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             AGGAAGGGGAAAATGATTTACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAC‑GTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGA‑TGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTT‑CTCTTTCACT‑GCGTACGCTACATCGGCAGCTTAAGCA‑GCAAACGGGACTG‑ACGCCTCAGCGATACCTGAACCGCCTGCGACTG‑ATGAAAGCCCGACATCTGCTACGCC‑ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACG  >  NC_000913/4098136‑4098599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑