| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,579,656 | A→C | 17.4% | V62G (GTG→GGG) | symE ← | toxic peptide regulated by antisense sRNA symR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,579,656 | 0 | A | C | 17.4% | 155.6 / 2.0 | 75 | V62G (GTG→GGG) | symE | toxic peptide regulated by antisense sRNA symR |
| Reads supporting (aligned to +/- strand): ref base A (50/7); new base C (6/10); total (56/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG > NC_000913/4579420‑4579888 | cgttgaaaTTTTGACTGGGGGGGGAAGTTTCTTTTTGGTCAGGGAAAGT‑CTCCCGGTGGTTAAGCGGGTATCAACGCCGTTACGCTACTCTTTGTTCACCGGCAATCACTCCAATAAACGGCTGCCCCTGCTTTTGTTTACGCGGCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑t < 2:890758/234‑1 (MQ=255) ggaaaTATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGCGAGGC‑ACATACAGCCTCCCATCAC‑ctt > 1:1006938/3‑238 (MQ=255) gTAAATTTTTTGTGGGGGGGAATTTTTTTTTTGGGTAGGGGGAAGG‑CCCCCGGTGGTTAACCGGGAGTAAACGCTTTTACGCGTCTTTCTTTTTACCGGCAATCACTCCAATAAACGCCTGCCCCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTg < 2:926349/239‑1 (MQ=255) atTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATc > 1:1007879/1‑238 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTCACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGCCAGTTTGCACACCGGCCGTAGCGACTGCATCCGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGGGGA‑CCATACAGCCTTCCCTCCC‑TTTC‑ACATccccc > 2:832531/1‑234 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTAcc > 1:54533/1‑238 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGGTGGGCGGTGAGGA‑CAAAACAGCCTTCCATCAC‑TTTG‑ACATCTAcc > 2:378317/1‑238 (MQ=255) gggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGGGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGt > 1:486857/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAATG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTg > 1:935128/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGTCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCCC‑TTTG‑ACACCTACCGCCGTg > 1:1075774/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTT‑ACATCTACCGCCGTg > 1:709749/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGCGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTg > 1:122408/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCCCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCACCAC‑TTTG‑ACTTCTACCGCCGTg > 1:511187/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCAACCCTGGCGTAGCGACTCCATCATTTCGCCCTCCTCGGCGGCGGGTGGTTGGGCGGGGAGGG‑CAATACAGCCTTCCCTACC‑TTTG‑ACACTCAccgcccgg > 2:265671/1‑235 (MQ=255) tttCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCGGTGGCa > 1:693854/1‑239 (MQ=255) tcgtgtggggATGG‑TTCGCGGTGTTTACCCGTGAGGAAACGGTGTTACGGGTCTTTTTGTTTAGCGGCAATCATTTCAATGAACGGCTACCCCTGCTTTTGTTGACGCGCCGACGGTGGGCAGCCCTGGCGGAGCGACTGCATCAGTTCGCCCTCCCCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTCCCACCTC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACcggc < 2:42875/231‑1 (MQ=255) gTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGGGGCAAAACcggcg > 1:694159/1‑238 (MQ=255) tCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACCGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTGGGGCGGTGCGGA‑CAATACAGCCTTCCATCCCTTTTG‑AC‑TCTACCGCCGTGCCAGGGGCAAAACccgcg > 2:857130/1‑237 (MQ=255) gTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGAtt > 1:470786/1‑238 (MQ=255) gTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGGGAGGA‑CAAACCAGCCTTCCATCAC‑TTTG‑ACATCTCCCGCCGGGCCAGGGGCAAAACCGGGGGCtc > 2:643024/1‑237 (MQ=255) ggAAGGCCTCCGGGTGTTTACCCGGGATAACACGCTTTTCGCCGTTTTTTTTTTTCCCGGCAATCCCCCCAATAACCGCCTGCACCGGTTTTTTTTTACCCGCCGACGTTTGGCCCCCCTGGCGTAGCGACTGCATCAGTTCCTTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑AAATCCACCCTCCCATCCT‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc < 2:873719/239‑1 (MQ=255) cTCCCGTGGTTTAACGGGGTGTAAACTCGTTTCCGCGTTTTTTTTTGTACCGGCAGCCCCCCCAATAACCGCCGGCACGTGTTTTTGTTTACGCGCGGCACGGTGGCACCCCTGGCGTGGCGACTGCATCAGTCCGCCCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑AAATACAGCCTTCCACCCC‑TTTG‑CCATCTCCCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACt < 2:529107/239‑1 (MQ=255) tCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGC‑CAATACAGCCTTCCCTCCC‑TTTT‑ACATTTCCAACCCTTCCACTGCCCAAACCGGCACCTTCCCCCCAcac > 2:108347/1‑237 (MQ=255) ccccGGTGGTTAACCGGGAGAAACCGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACCCCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTg < 1:378317/238‑1 (MQ=255) ccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGCGGTTGGGCGGTGAGGA‑CACTACAGCCTCCCATCAC‑TTTG‑CCATCTACCGCCGGGCCAGTGGCAAAACCGGGGGCTTCCAccccccgc > 2:736146/1‑233 (MQ=255) ccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTACACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGa > 1:119183/1‑238 (MQ=255) aCCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGt > 1:958565/1‑239 (MQ=255) cttgtccccgATTTTCTGTTTACCGGCAATCCCCCCAATAACCGCCGGCCCCTGCTTTTGTTTACGCGCCGACAGTTTGCACCCCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATAc < 1:721855/231‑1 (MQ=255) tGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑CAATCTACCGCCGTGCCAGTGGCAAAACCGCGGGCCTCCCACCGCAGACCCTTCCGGGGGATGGCggggaaaag > 2:665616/1‑233 (MQ=255) tGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCATCGGCGGCGGGGGGTTGGGCGGTGAGGC‑CAAATCAGCCTTCCATCAC‑TTTG‑ACATCAACCGCCGTGCCAGTGGCAAAACCGCGGGCTTCCCGCCCCAGACATTTCCGGGGGGAGGGGGGAAAACg > 2:84054/1‑239 (MQ=255) gTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGGGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACg > 1:462987/1‑238 (MQ=255) cgcgACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGGGAGGC‑CAATACAGCCTTCCATCCC‑TTTT‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGaaaagggggga > 2:1082988/1‑230 (MQ=255) cTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGCCCTTTCAGGGTGATGGCGGGAATACGGCTGTAATcc > 1:339286/1‑239 (MQ=255) cTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAgg > 1:64213/1‑212 (MQ=255) cTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAgg > 1:256053/1‑212 (MQ=255) cTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGACTACGGCTGTAATcc > 1:193940/1‑239 (MQ=255) cTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATcc > 1:959403/1‑239 (MQ=255) ttCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGCCCTTTCCGGGGGATGGCGGGAATACGGGGGTAAACCgg > 2:1075518/1‑239 (MQ=255) aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGTCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCCGCCCCTGCCCTTTCAGGGTGATGGCGGGAAAACCGGCGTAAACCCGGTG‑Gcaa > 2:635313/1‑236 (MQ=255) aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAgg > 1:809830/1‑202 (MQ=255) aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCATGGTGATGGCGGGGATACGGCTGTAATCCGGGTA‑GCGa > 1:880391/1‑238 (MQ=255) cAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCa > 1:464867/1‑239 (MQ=255) aTCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCCGGGGGATGGCGGGAAAACGGCTGTACACCCGGTA‑GAGAcacacaaa > 2:171985/1‑232 (MQ=255) tCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACCCGCATAAcct > 1:365709/1‑237 (MQ=255) gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGCCGGGGGCATg > 1:1000026/1‑239 (MQ=255) gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGGA‑GCGACTCGCATAACCGCCGGTGACATg > 1:937637/1‑239 (MQ=255) cTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCCAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGCATACGGCTGCAATCCGGGTA‑GCGCCTCGCCTAACCGACgggtccttgac > 1:1620/1‑230 (MQ=255) ttccgcgCCGACAGTTGCCCCCCCGGGCGTGGCTACTGCATCATTTCGCTCTCCTCGGCGGGGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTGG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGga < 1:857130/236‑1 (MQ=255) ttccgccgcGACGGTTTGCACCCCTGGCGGAGCGCCTCACACGGTTCGCCCTCCCGGGCGGCGGGTGGTTGGTGGGTGAGGA‑AAATGCAGCCTTCCATCCC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATTCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGga < 1:84054/231‑1 (MQ=255) tttGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGCCGGGGACATGACGGTTATTTGCCCGGGAGACT‑TCTGCTTCgg > 1:778265/1‑238 (MQ=255) cacCGGGCGTAGCGACGGACTAAGTTCGCTCTCCGCGGTGGGGGGTGGTTGGGCGGTGAGGA‑CAATGCAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGtt < 1:189184/239‑1 (MQ=255) tGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGaa < 1:123540/151‑1 (MQ=255) tGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGaa > 2:123540/1‑151 (MQ=255) tGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACC‑TCTGCTTCGAACGGTTGTGCAATacctggc > 1:861926/1‑231 (MQ=255) gCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGCGATGGCGGGAAAACGGCTGGAATCCggg > 1:24066/1‑155 (MQ=255) cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGGTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAAAATGCgtg > 1:156695/1‑238 (MQ=255) tcggTTCTCTCCTCTCGGGGGGGGGTGGTGGGGGGGGGGGAA‑ACACACGGCTTCCCACCCT‑TTTG‑ACTTCCACCGGCGGGCCAGGGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTgtca < 2:1000026/236‑1 (MQ=255) tCGCTCTCCGCGGCGGGGGGTGGTTGGGCGGTGAGGA‑CAAGCCGGCCTTCCATCCC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCAt < 1:573500/239‑1 (MQ=255) cgattcccctgGGCGGCGGGTGGTTGGG‑GGGGAGGACCAGTGTCGCC‑TCCACACC‑TTTGCACCCCTCCCCCAGTGCCAGTGGCAAACCCGGGGGCTTCCAGCCACTGGCCTTGCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCAt < 2:1620/232‑1 (MQ=255) ctctCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGGTGTAATCCGGGTA‑GCGACTCGGCTAACAGACGGGGACCAGACGGTTAATTTCCGGGGGGGCTCTCTGGTCCAAAC‑GTTGGGCAATAACAAGCgtgtagcgtaaaa > 2:209094/1‑229 (MQ=255) ctcGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGGACGGTTGTGCAATAGAATGGGGGTCAGTCATAACTGCTa > 1:464952/1‑239 (MQ=255) tgggcgggggTGGGTTGGGCGGGGAGGA‑CAATACCGCCTTCCATCTC‑TTTG‑CCACTGCCCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTAt < 1:67347/237‑1 (MQ=255) ggCGGTGAGGG‑CAATACAGCCTTCCATCAC‑TTTG‑ACCTCTACCGCCGTGCCAGGGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCCGGGGGATGGCGGGAATACGGCTTGTATCCGGGGACGCGCCCCCCTAAACGCACGGGGACA‑GGCGGTTATTTGCCGGGGGGGCT‑TTGTCTTCGTACGGGTGTGAAATAGAATGCGGGTCCGTCCACACTTCCATTTTCCAGGGAAAgggat > 2:154815/1‑239 (MQ=255) gggtggagaaaaTCCACCCTCCCAACCC‑TTGG‑ACACTGACCGGCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAgtgatt < 1:271191/228‑1 (MQ=255) tGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATa > 1:1052788/1‑191 (MQ=255) aGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGCCGGTGACATGACGGGTATTTGCCGGGGGGACT‑TCTTCTTCGAACGGTTGTGCAATAGAATGCGGGTCAGCATTAACTGCTATTTTCCCGGAATAgggattgggttt > 2:795559/1‑234 (MQ=255) aGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGGA‑GCGACTCGCATAACCGGCGGGGGCATGACGGTTATTTGCCGGGGAGAAT‑TCTGCTTCGAACGGGTGTGCCATAGAATTGGGGTCAGCCATAACTGCTATTTCCCCGGAAAAgggatttgggtt > 2:756400/1‑233 (MQ=255) aGGA‑AAATACCGCCTCCCATCCT‑TTTA‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAgtgattgtgat < 1:795559/238‑1 (MQ=255) tACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATa > 1:807645/1‑182 (MQ=255) tACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTTTCCAGGAATAGTGATTGTGATTAGCGAtc > 1:299321/1‑238 (MQ=255) acacCCTACCATCCT‑TTTG‑CCTTTCACCGCCGTGCCAGTGGCAAACCCGGGGGCTTCCAGCCACTGCACTTGCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGc < 1:385485/239‑1 (MQ=255) cgCCTTCCATCCT‑TTTG‑ACATCTGCCGGCGTGCCGGTGGCAAAACCGGGGGCTTCCAGCCAATGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCgg < 1:108347/238‑1 (MQ=255) ccTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACgg > 1:682475/1‑124 (MQ=255) tCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGCACGGTTGTGCAATAGAATGCGGGTCAGTCATAACTGCTATTTTCCAGGAATAGGGATTGTGATTAGCGATGCggggggg > 1:384197/1‑235 (MQ=255) tCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCCGTCATAACTGCTATTCTCCAGGAAAATGGATTGGGATTACCGATGCGGgggtg > 1:506020/1‑239 (MQ=255) | CTGGTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGG‑CTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGA‑CAATACAGCCTTCCATCAC‑TTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACGGCTGTAATCCGGGTA‑GCGACTCGCATAACCGACGGTGACATGACGGTTATTTGCCGGGGAGACT‑TCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG > NC_000913/4579420‑4579888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |