Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.90.2% 260.3 / 12.1 71pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base . (57/7);  total (60/11)
Fisher's exact test for biased strand distribution p-value = 9.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.90.4% 261.0 / 8.9 71pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base . (57/7);  total (60/11)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCAC  >  NC_000913/2173125‑2173591
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                     
atgaGCCGCCAGTGGGTTGTGCAACCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCCCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccacc                                                                                                                                                                                                                                       <  1:518823/235‑1 (MQ=255)
         cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACAGCAATTAAAATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATaccaccgccggatgc                                                                                                                                                                                                                              >  2:882743/1‑229 (MQ=255)
         cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAAcccccgtatgg                                                                                                                                                                                                                              >  1:307255/1‑231 (MQ=255)
         cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGAAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGATACCAaccccgtatgg                                                                                                                                                                                                                              >  1:712399/1‑231 (MQ=255)
            ttGGGTTTGCAAACCAATAGTTTGCGTCCCGATCCACAGGGTGATGCTTATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCAACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                                                                                                         <  1:875553/238‑1 (MQ=255)
                  gtgCAACCAAATGGTTGGGGTCGCGATCCAAGGGGGGATCCCCAAAATGATGCCACCCGAGATGAAGGTGCGGAAAAGATTTCCCCGAGACAGGGGGCCCGCCATCGCCAAGAAGAAGCCGATGGGGGAAAGACCGCCAAACGGCAGCACCTGATTCCCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                   <  2:800788/238‑1 (MQ=255)
                     cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGGTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAAc                                                                                                                                                                                                                >  1:1052450/1‑238 (MQ=255)
                                  tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCTACAGCCATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                  >  1:1318276/1‑239 (MQ=255)
                                  tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGCGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGCATTTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                  >  1:701860/1‑239 (MQ=255)
                                  tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGTTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGcc                                                                                                                                                                                                  >  1:315719/1‑239 (MQ=255)
                                  tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                  >  1:632330/1‑239 (MQ=255)
                                  tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGcc                                                                                                                                                                                                  >  1:870953/1‑239 (MQ=255)
                                    cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCAACCA‑‑CCGCCGCATGTCCCAGCAGAAACGCCGGACCAAGGCCaa                                                                                                                                                                                                >  1:206637/1‑239 (MQ=255)
                                    cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGAACCCCCCCCCGTATTTCCCAGCCGCAACCCCGggccaaggccaa                                                                                                                                                                                                >  2:1213065/1‑239 (MQ=255)
                                    cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGGTGAGTGGGATAAAAATCAGCCTTGCCGTTACCACCC‑‑CCGTATGTCCCAGCAGCAACCCCGGATCAAGCCCa                                                                                                                                                                                                 >  1:1289944/1‑238 (MQ=255)
                                                      gATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCACCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                                                                                                                               >  1:821617/1‑238 (MQ=255)
                                                       aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                             >  1:89114/1‑239 (MQ=255)
                                                             aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCC‑‑CCGTAGGTCCCAGCAGCAACGCCGGACCAAGGCCAAACAGGAACCCCCAACCCCCGAACt                                                                                                                                                                        >  2:5655/1‑238 (MQ=255)
                                                             aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACt                                                                                                                                                                        >  1:1108914/1‑238 (MQ=255)
                                                             aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                        >  1:1199541/1‑238 (MQ=255)
                                                               aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCGCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                      >  1:964426/1‑238 (MQ=255)
                                                               aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCCGGAACCCCTGACCGCCGAACTTc                                                                                                                                                                      >  1:276178/1‑238 (MQ=255)
                                                               aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CAGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGACACTCCTGACCGCCGAACTTc                                                                                                                                                                      >  1:1359743/1‑238 (MQ=255)
                                                                tggtgaccccccGAGTTAAGGGGGGGGAAAGATTTTCCAGGATGCCGGGGACCCCCACTCCCCAGAAAAACGCCGAGGGGGGCAAGATCGCCAAGGGGCAGCACGTGATTACCCGCCACAAAAACAGCAATAAAAATGGTGAGGGGAAAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                                                                                                                    <  2:89114/230‑1 (MQ=255)
                                                                      acacCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                                                                                                                              >  1:705511/1‑239 (MQ=255)
                                                                             agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                                                                                                        >  1:969253/1‑238 (MQ=255)
                                                                                  aaGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                                                                                                                   >  1:274046/1‑238 (MQ=255)
                                                                                  aaGGGGCGAAACAGTTTACCCCGAGCCACGGCAACCGCCATCGCCACGAAAAAGCCGATGGTGGCAAGATCGCCAAACGACAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                  <  1:894285/239‑1 (MQ=255)
                                                                                        cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCCGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGCCAACACGACTGCtt                                                                                                                                            >  1:605019/1‑239 (MQ=255)
                                                                                         ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATACAAATCAGGCTTGCCGAAACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGAACAAGGCCAATCAGGAACTCCTGCCCGCCGCACTTCGCCCGGAAACAACCACCACCCTGCTTa                                                                                                                                           >  2:918042/1‑239 (MQ=255)
                                                                                         ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCCCCCCACTTCGCCTGGAAAACAACACGACCCCGCTTa                                                                                                                                           >  2:342215/1‑239 (MQ=255)
                                                                                             cAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                                                                                                       >  1:1265234/1‑239 (MQ=255)
                                                                                                   ttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGCTgggg                                                                                                                                  >  1:944243/1‑238 (MQ=255)
                                                                                                      cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGCGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCGGCTATGCGATGGGCGtt                                                                                                                               >  1:1365407/1‑239 (MQ=255)
                                                                                                           tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcc                                                                                                                          >  1:490802/1‑239 (MQ=255)
                                                                                                           tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACAAGCCTGCTTAGCGATAGGCGTTAAccc                                                                                                                          >  1:482605/1‑239 (MQ=255)
                                                                                                             cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCACCGAACTTCGCCTGTAAACGACTAAGAGCCTGCTTAGCg                                                                                                                                        >  1:972879/1‑223 (MQ=255)
                                                                                                             cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTAAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                        >  1:25901/1‑239 (MQ=255)
                                                                                                                    cccGCCAACGCAACAAAAAAGCCGATGGTGGCAAGATCCCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                 <  1:222073/238‑1 (MQ=255)
                                                                                                                    caccgcatgccccACGAAGAAGCCGATGGTGGCAAGAGCGCCAACCGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                   <  1:882743/227‑1 (MQ=255)
                                                                                                                     ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTCCTTAGCGCTGGGCGTTAAACCATCCctgatg                                                                                                                >  1:843598/1‑239 (MQ=255)
                                                                                                                     ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCCCCTGTAAACGACAACGAACCGACTTAACGCGAGGCGGTAAAcaatccataaag                                                                                                                >  2:291634/1‑235 (MQ=255)
                                                                                                                     ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTCCTTAGCGATGGGCGTTAAACCATCCatgatg                                                                                                                >  1:226090/1‑239 (MQ=255)
                                                                                                                       accATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAGGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGACCTTCGCCTGTAAACGACTACAAGCCTGCTTAGGGATGGGCGTTAAACAATCCATAATggg                                                                                                              >  2:703345/2‑239 (MQ=255)
                                                                                                                             ccccACGAAGAAGCAGGGGGGGACAATCGCCCCAAGCGGAAGCCCCAAATGCCCGGGCACAAAAACAGCAGTAAAAGGGGTTAGTGGTAAAAAAAGCAGGTTGGGCGAACCCTCCGCCGTCTGCACCAGCAGCAACGCCGGATCAGGGCCAACCCGGAACTCCTGACCGCCCAACTGCGCCTGTAAGCGGCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa                                                                                                         <  2:937776/237‑1 (MQ=255)
                                                                                                                             cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa                                                                                                         >  1:895589/1‑239 (MQ=255)
                                                                                                                                   gaaaaggCCGAGGGGGACAAGACCGCCAACGGCAACCCCTTGATACCCCGGCAAAAAAACAACAATAAAAATGGGGAGGGGGAAAAAAACCGGGCTTGCAGATCCCCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                   <  2:1108914/233‑1 (MQ=255)
                                                                                                                                         gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCAGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATAAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACAACAACGAGACTGCTTAGCGATGGGCGTTACACCACCCAGCTTGGGTTTAATCAccccggg                                                                                               >  2:778206/1‑233 (MQ=255)
                                                                                                                                          ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                            >  1:677143/1‑239 (MQ=255)
                                                                                                                                                        aTCGCCAAACGGCGGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCGGAAAACGACTACGACCCTGCTTAGCGATGGCGGTTAAACAATCCATGCGGGTTTAAATCCCCCGGGGCATTaggccatccact                                                                               >  2:96785/1‑228 (MQ=255)
                                                                                                                                                             cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATTACCCCGTGCATTACCACCATCACTaccgcc                                                                         >  1:1320694/1‑239 (MQ=255)
                                                                                                                                                             cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgcc                                                                         >  1:1027478/1‑239 (MQ=255)
                                                                                                                                                               aaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAAACCCACCATGATGGGTTTAATCACCCGGGCCATTAACAGCACAACTGCCCCCGt                                                                       >  2:1115289/1‑239 (MQ=255)
                                                                                                                                                                  ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACAAGCCTACTTAGCGATGGGCGTTAAACCATCCATGATGGATTTAATCACCCGTGGCATTACCAGCATCACTGCCGCCGtttt                                                                    >  1:642652/1‑239 (MQ=255)
                                                                                                                                                                         ccTGATTACCGGGCACAAAAACAAAAATTAAAATGGTGAGGGGAAAAAAAACCAGGTTTCCCGATCCCCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                             <  1:703345/239‑1 (MQ=255)
                                                                                                                                                                            tgtttcccccGCCCACAAACAGCAAATAAATGGGGGGGGGGAAAAAAAACACGGTTTGCCAAGCCCTCCGCTGTAGTCCCCAGCAACAACGCCGGACAAAGGCCAATCGGGACCTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCTTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                           <  2:870953/233‑1 (MQ=255)
                                                                                                                                                                                     ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGGGCGTTAAACCACCCATCATGGGTTTAATCACCCGTGGCATTAGCAGCATACCTGCCGCCGTTTTTCCCCCCAGCTGCAATa                                                   >  1:988354/1‑238 (MQ=255)
                                                                                                                                                                                     ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                   >  1:1280445/1‑238 (MQ=255)
                                                                                                                                                                                     ggCACACAAACAGAATTAAAATGGGTGAGTGAGATAAAAATGAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGAAACGCCGAATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                  <  1:586868/239‑1 (MQ=255)
                                                                                                                                                                                       cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                        >  1:744844/1‑151 (MQ=255)
                                                                                                                                                                                                    aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                   >  1:972246/1‑239 (MQ=255)
                                                                                                                                                                                                    aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATAAACCTTTGACATCGTa                                   >  1:397419/1‑239 (MQ=255)
                                                                                                                                                                                                    aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACAATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTTCCGCCGTTTTTACCGCCCGCTGCAGTAAACCTTTGAc                                         >  1:789677/1‑233 (MQ=255)
                                                                                                                                                                                                          aTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGAATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACAACGTAAACcgc                             >  1:627134/1‑239 (MQ=255)
                                                                                                                                                                                                              gTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATAAACCTTTGAAACCGTAACCCCCGAg                          >  2:576024/1‑239 (MQ=255)
                                                                                                                                                                                                                gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACCCCTTTGACCTCGTAACCCGCGAGGa                        >  1:1320530/1‑239 (MQ=255)
                                                                                                                                                                                                                 aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCACCTCCAATACACCTTTGACACCGAAACCCCCGAGGa                        >  2:898788/1‑238 (MQ=255)
                                                                                                                                                                                                                   tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGGCTTAAACCATCCATGATGGGATTAATCACCCTTGGCATTAGCAGCATCACTACCGCCGTTTTTACCGCCACCTGACAAAAACCTTTGACACCGAAAccccccagaaag                      >  2:99876/1‑229 (MQ=255)
                                                                                                                                                                                                                   tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGCCATCGTAACCCGCGAGGAtc                      >  1:1313096/1‑237 (MQ=255)
                                                                                                                                                                                                                   tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATg                      >  1:138915/1‑238 (MQ=255)
                                                                                                                                                                                                                           aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGGCATCGTAACCCGCGAGGAACCCGCTaacc             >  1:474193/1‑237 (MQ=255)
                                                                                                                                                                                                                               cAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGCTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCCAGGATGCCGAAAATCAGc          >  1:1019522/1‑238 (MQ=255)
                                                                                                                                                                                                                                aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGAAACCCGCAAGGATCCCGaaaacaacccc        >  2:1311890/1‑232 (MQ=255)
                                                                                                                                                                                                                                      tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTCGAATACACCTTTGACATCGTAACCCCCCAGGAGGCCGGTAATcctttcccttt   >  2:1100510/1‑229 (MQ=255)
                                                                                                                                                                                                                                       gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTAATCACCCGTGGCATTAGCAGCATCCCTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCCTAACCCCCCAGAATCCCGTTAATAAGCCCCTTcac  >  1:505426/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                     
ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCAC  >  NC_000913/2173125‑2173591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: