Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,975,093 T→G 18.4% T308P (ACT→CCT)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,975,0930TG18.4% 120.5 / 3.1 72T308P (ACT→CCT) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base T (32/25);  new base G (12/3);  total (44/28)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTGGGG  >  NC_000913/2974859‑2975326
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
tATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGTGAAACTGGCGGGAAGGGaga                                                                                                                                                                                                                                       >  1:863325/1‑237 (MQ=255)
 ttGGACGTCGCCTTTTGGATGGCCTTCAGAACCGGAGGTAAAAAGGAGCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGa                                                                                                                                                                                                                                      <  2:319594/238‑1 (MQ=255)
 aTGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGa                                                                                                                                                                                                                                      <  1:700510/239‑1 (MQ=255)
   ggACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATg                                                                                                                                                                                                                                    >  1:1134499/1‑239 (MQ=255)
         cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGtgttt                                                                                                                                                                                                                              >  1:1365746/1‑238 (MQ=255)
         cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAGAATGGGTTTg                                                                                                                                                                                                                              >  1:1088023/1‑239 (MQ=255)
         cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAAGGAAGATGGGTTTg                                                                                                                                                                                                                              >  1:1252565/1‑239 (MQ=255)
         cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGGAAGTTGCTCAGGCAGATGCCCAAGGTTGCCTTTATCGAGAAAATGGGGGAGAGTGAAAAAGGGTTTg                                                                                                                                                                                                                              >  2:569594/1‑239 (MQ=255)
                  gATGGCTTTGAGAACCGGAGGAAAAAAGGATCAGGGCCTCTTCTTCCGGCTGCTTTTAAACCTGTGCAAGACGGGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgc                                                                                                                                                                                                                      <  1:584912/238‑1 (MQ=255)
                    ttgCCTGCAGACCCGGGGGAAAAAAGGATCCGCCCCCTTTCTTCCGGTGGCTGTTTAACCCGGGCCAGCGGGGGCATCAACAAATGAGCAAAGATCCATTCTTGGTCGGCAGTGGGTTCATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACTGGAGTAAGTTGCTCCGGGAGATGCCAGAGTTTGCCTTTATCGAGAAGCTGGCGGGAAGTGAAGATGGTTTTGATTGCAgccgc                                                                                                                                                                                                                   <  2:1134499/237‑1 (MQ=255)
                     ggtcTTCAGACCCGGGGGTAAAAGGGTCTGCGCCTCTTTCTTCCGTCTCTTTTTAAACCTGTGCCAAACGCGGCATGACCAAAGGGGTGAATCTCAATTCTTTGTCGGCAGTGGTGACATCTGTTTTTAAATCTTCCAGATAGCCCCAGCGCATCGGAGTAACTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGAAAGTGAAGATGGTTTTGATTTCAgccgc                                                                                                                                                                                                                   <  2:595937/235‑1 (MQ=255)
                     ggCCTCCAGACCCGGAGGAAAAAAGGGACCGCCCCCTTTCTTCCGGGGTGTGTTTACCCTGGGCCGGACGGGGAAACCGCAAATGAGCGAAGAACCATTCTTTGCCGGCAGTGGTGACATCTGCTTTAAAATCTTCCAGATAGACCCAGCCCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAAAAACGGGCGGGAAGTGAAGATGGATTTGATTTCGgccgc                                                                                                                                                                                                                   <  2:1088023/238‑1 (MQ=255)
                     cgctttCAGAACCGGGGGTAAAAGGGACCAGCACCTCTTCTCCCGGCTGCTGTTTACCCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATATTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGCCCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGATTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                                                                                                                                                                   <  1:1177197/234‑1 (MQ=255)
                      gCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGGTTTTAAccgccg                                                                                                                                                                                                                 >  1:24322/1‑239 (MQ=255)
                      gCCTTCAGAACCGGAGGTAAAAAGGATCAGCACCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATCCTTTGTCGGCAGGGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGGGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGGAGTGAAGAAGGGTTTGtttttcgcccccc                                                                                                                                                                                                                 >  2:1177197/1‑230 (MQ=255)
                                ccGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGc                                                                                                                                                                                                          >  1:397461/1‑236 (MQ=255)
                                   gagtaaaaaaTGCTCAGCGCCTCTTCTTCCGGCTGATGTTTACCCGGTGCCAGACGCGGCATGAGAAAATGAGCGAAGTTCCTTACTTTGTCGGAAGTGGTGACATCTGTTTTAAAATCTCCCAAATAGCCCCAGCGCACATGAGTAGGTTGCTCCGGCAGATGCCAGAGTTTCCCTTTATCGAGACACGGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGt                                                                                                                                                                                                    <  2:1253487/234‑1 (MQ=255)
                                      gTAAAAAGGATCAGCGCCTCTGCCTCCGGCTGCTTTTTAACCTGGGCCAGACGGGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTAAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTaa                                                                                                                                                                                                              <  1:276567/226‑1 (MQ=255)
                                       tAAAAAGGCTCCGCCTCTCTTTTTCCGGGTTCTTTTTAACCGGGGCCAGACGGGGCATCAAAAAATGAGGGAAGCCCCATCTTTGGGGGGAAGGGGTGACATCTGCTTTAAAATCTCCCAGATAGCCCCAGCGCCCCTGAGTAAGTTGCTCGGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACGGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                                                                                                                                                                 <  2:24322/238‑1 (MQ=255)
                                       tAAAAAGGATCAGGCCCTCTTCTCCCGGCTGCTGTTTACCCTGTCCCAGCCGTGGCATCAGCAAATGAGCGAATATCCATACTTTGTCGGCATTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAGGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATGGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                                                                                                                                                                 <  1:569594/238‑1 (MQ=255)
                                       aaaaaaGGATTCGCGCCTCTTCTTCCGGCTGCTTTTTACCCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTAACCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                                                                                                                                                                 <  1:866889/237‑1 (MQ=255)
                                                tCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTAc                                                                                                                                                                                        >  1:954564/1‑238 (MQ=255)
                                                  cccgtccctttcttcCGTTTGTTGTTTCACTGTGGCCAGATGGGGAATCAGCAAATGAGCGAAGTTCCATACTTTGTGGGCAGTGGTGCAATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGAAGATGCCAGATTTTGCTTTTATCGAGAACCTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTAccc                                                                                                                                                                                      <  2:635548/230‑1 (MQ=255)
                                                    cgcCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGGTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGAAGGATTTGATTTCCACCGCCGTAATAACAATTGTCAGCCCTTTTAcccccg                                                                                                                                                                                   >  1:1063955/1‑237 (MQ=255)
                                                     gcCTTTTCTTCCGGCTGCTTTTTAACCTGTGCCAGACGGGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCgg                                                                                                                                                                                   <  1:592011/238‑1 (MQ=255)
                                                          tttcttcCGGCTGCTGTTTAACCTGTGCCAGGCGGGGAACCAGCAAATGAGCGAAGATCCATTCTTTGTCGGCAGGGGTGACATCTGCTTTTAAATCTTCCAGAGAGCCCCAGCGCCCCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAgtg                                                                                                                                                                              <  1:798739/238‑1 (MQ=255)
                                                               tcCGGCTGCTGTTGAACCTGGGCAGGACGCGGCATCAGCAAGTGAGCGAAGATCCATACTTGGGCGGCAGGGGTGACATCTGCTTTTAAATCTTCCAGATAGACCGAGCGCACCTGAGTAAGTGGCTCCGGCAGATGCAAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGtt                                                                                                                                                                         <  1:705333/239‑1 (MQ=255)
                                                                   gctgctGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAATGGGAGAGGGGTTTGATTTTAGCCCCCGGAAAAACACTGGTCAGCCCTTTTACCCCCGCAGGGGAGtttcaa                                                                                                                                                                     >  2:1152100/1‑235 (MQ=255)
                                                                       ctgTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCCCCGTAATAGCCCTGGTCAGCCCTTTTACCCCGGCAGGGTAGTTCATCAtt                                                                                                                                                                  >  1:631718/1‑238 (MQ=255)
                                                                                   tgCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCATTGTAGTTAATCATTGCGGGCCTgcgcc                                                                                                                                                     >  1:608131/1‑237 (MQ=255)
                                                                                   tgCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGAGGGTTTTGATTTCAGCCGCCGTAAAACCCCTGGTCAGACCTTTTACCCCGGCAGGTAAGTTCATAATTCCTGGCAAgcggc                                                                                                                                                     >  1:538915/1‑239 (MQ=255)
                                                                                     gCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTTATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGGGTAGTtcatc                                                                                                                                                                     >  1:1101414/1‑222 (MQ=255)
                                                                                     gCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGGATTCAACCCCCGTAATTGAACTGGGCAGGCCTTTTTACCCGCCAGGTTGATTCATCAATGCGGGCAGtcggc                                                                                                                                                     >  1:1049684/1‑238 (MQ=255)
                                                                                         gACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGCCgg                                                                                                                                                >  1:64262/1‑239 (MQ=255)
                                                                                         gACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTATTGATTTCAGCCGCCGTAATAGCACTGGTCAGGCCTTTTACCCCGGCAGGTTAGTTCATAATTGCGGGGCAGCGGCCACgg                                                                                                                                                >  1:1057564/1‑239 (MQ=255)
                                                                                          aCGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGGGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTACGCCGCCGTAATAGCACCGGCCAGCCATTTTACCGCGGCAGTGTTGTTAATCATTTCGGGCATGCGGCGACggg                                                                                                                                               >  1:1309765/1‑239 (MQ=255)
                                                                                              ggCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGGGTAGTTCATCATTGCGGGCATGCGGCGACggggcat                                                                                                                                           >  1:822951/1‑235 (MQ=255)
                                                                                                          tGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCCTGCGGCCACGGGCGATGGCCCCAAAAAt                                                                                                                               >  1:362693/1‑239 (MQ=255)
                                                                                                                aaGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATAATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTg                                                                                                                          >  1:500811/1‑238 (MQ=255)
                                                                                                                   aTCAATACTTGGGGGGCAGTGGTGACATCTGCTTTTAAAGCTTCCAGATAGCCCCGGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACGGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTaa                                                                                                                                                                                                              <  2:410697/151‑1 (MQ=255)
                                                                                                                    tCCATACTTTGTCGGCAGTGGTGACATCTGCTTTAAAATCTTCCAGATGGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGGGGAAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCg                                                                                                                       <  1:1152100/237‑1 (MQ=255)
                                                                                                                      ccttttttGTCGGCGGGGGTGATTTTTGTTTTAAAATCTCCAAGATAGACCCGGGGCACCTGGTAAAGTGGCTCCGGAAGAGCCGAGATTTTTCTTTTAGCGAAAAACGGGGGGGAAGGGAAGTTGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                                                                                   <  2:954564/234‑1 (MQ=255)
                                                                                                                           tttGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTCAACCCCCGGAATAACACCGGTCAGCCCTTTTACCCCGGCAGTGTAATTCATAATTGTCGGCATGCGGCGACGGGCGATGGGCCCAAAAAACAAAGCCCCACGGaag                                                                                                               >  2:712074/1‑236 (MQ=255)
                                                                                                                           gttGTCGGAGGTGGTTATATTTGTTTGAAATTCTTCCAGAAAGCCCACGCTATCCTGAGTATTTGGCTCGGGTATAGGACATTCTTTTCCTTCATGGAAAAACGGGCGGAAGGTGAAGTTGGTTGTGATTTCAGCCACCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCTGCGACGGGCGATGGCCCCAAAATTCACTGCCGCGCTGATGc                                                                                                              <  2:1063955/238‑1 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGGAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCACCATTGCGGGCATGCGGCGACGGGCGATGGCCCCCAAAATCACTGCCGCACCGATGccc                                                                                                            >  2:964507/1‑238 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGt                                                                                                                                                                                                    >  1:410697/1‑151 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCCGATg                                                                                                               >  1:882174/1‑236 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGGAGTTCATCATTTCGGGCCTGCGGGGACGGGCGATGGCCCCACAAATCCCTGCCGCCCTGATg                                                                                                               >  1:1155469/1‑236 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGGAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCCACGGGCGATGGCCCCAAAAATCACTGCCGCACTTATg                                                                                                               >  1:1368633/1‑236 (MQ=255)
                                                                                                                             tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAAATGGTTTTGATTTCAGCCGCCGTAATAGCCCTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCCTCACTTCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCCCCCtgctgcg                                                                                                             >  1:652383/1‑237 (MQ=255)
                                                                                                                                    aGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATTCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCTGCATTgg                                                                                                     >  1:643143/1‑239 (MQ=255)
                                                                                                                                          tacttttttttttAAATCTTCCAGAAAGACCCAGCCGCTCTGAGGTAGTGTCTCCGGCAGATGCCAGAGTTCGCCTGTGACGAGAACCGGGCGGTAAGTGAATGTGGTTTTGATTTCGGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGCAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgcagca                                                                                               <  2:170437/230‑1 (MQ=255)
                                                                                                                                             aTCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGGAATAACACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATAACTGCCCCACTGATGCCTGCATTGGGCAGCCTTa                                                                                            >  1:772580/1‑239 (MQ=255)
                                                                                                                                                tGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACCGGGCATGGCCCCCAAAATCCCTTCCGCCCTGATGCCGGCAATGGGGAGCATTaaa                                                                                          >  2:209553/1‑237 (MQ=255)
                                                                                                                                                  cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTCTCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCCGCCGCCGTAATAGCGCTGTGCAGCCCTTTTACCCCGGCAGTGTAGTTCTTCTATTCGGGCAGCCGGCGCCGAGCGGTGGCCCCCAAAATCACTGCCCCACTGAGTCCGGCATTGGGAAGAATTAAGCCg                                                                                       >  2:531398/1‑239 (MQ=255)
                                                                                                                                                  cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtcat                                                                                                                                                                      >  1:131000/1‑160 (MQ=255)
                                                                                                                                                     ttAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTCAGCCTCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGGGTAGTTCATCAATGCGTGCAGGCGGCGACGGGCGATGGCCCCAAAACTCACTGCCGGACTGCTCCCGGCACAGGCCCGCATTAAACCgctc                                                                                    >  1:273941/1‑236 (MQ=255)
                                                                                                                                                       aaaTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCCTGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCTGCCTTGGGCAGCATTAAGCCGCTGCg                                                                                  >  1:1207205/1‑239 (MQ=255)
                                                                                                                                                                    agccccAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAaca                                                                     <  1:964507/236‑1 (MQ=255)
                                                                                                                                                                       cccGGCGCCCGTGAGGAAGTTCCCCCGGCAGATTCCAGTGTTTGCCTTTATCGACAACCTGGCGGGAAGTGAATATGGTTTTGTTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAacac                                                                    <  1:1277170/237‑1 (MQ=255)
                                                                                                                                                                         cAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGGGGGGTTTGATTTTAGCCGCCGTAATAGCACTGGTAAGCCCTTTTAACCCGGCAGGGTAGTTCATCATTGCGGGCATGCGGCGACGCGCGATTGCCCCAAAAATTCATGCCGCACTGCTGCCTGCATTAGGCAGAAATAAACCGATGCGTTCGCCTTCAACCCTGTa                                                                >  2:961557/1‑239 (MQ=255)
                                                                                                                                                                           gcgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGGAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCCCCCTGATGCCTGCACTGGGCAGCATTAAGCCCAGCCGTTCGCCTTCAAAACTGTAtt                                                              >  2:757122/1‑239 (MQ=255)
                                                                                                                                                                           gcgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGGTTTGATTTCAGCCGCCGTCATAGCACTGGTCAGCCCTTTTAACCCGGCAGTGTAGTTCATCCTTGCGGGCATGCGGGCACGGGCGATGGCCCCCAAAATCACTGCCGCCCCTATGCCTGCCTTGGCGCGCATTTAACCGCAGCCGTCTCCTTCCAAACTGTAtt                                                              >  2:51767/1‑239 (MQ=255)
                                                                                                                                                                                      tAAGTTGCTCCGGGAGGTGCCAGGGTTCGCTTTTATCGAGAAACTGGCGGGAAGTGAAATTGGTTTTGTTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGAt                                                    <  1:900072/238‑1 (MQ=255)
                                                                                                                                                                                      tAAGTTGCTCCGGCAGGTGCCATGGTTTGCCTTGAACGAAAAACGGGCGGGAAGGGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGAt                                                    <  1:1350536/238‑1 (MQ=255)
                                                                                                                                                                                         gTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCCAAAAACACTGCCGCACTGATGCCCGCATTGGGGAGCCTTAAGCCCATGCGTTCCCCTTCCACACTGTCTTTTTTCAGGaatgg                                                 >  2:1350536/1‑234 (MQ=255)
                                                                                                                                                                                              tCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTTGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGCATTTTTCAAGGATGCGTCcaac                                           >  1:432076/1‑239 (MQ=255)
                                                                                                                                                                                              tCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGGGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCCACACTGTATTTTTCAAGGATGCGTCcaac                                           >  1:871227/1‑239 (MQ=255)
                                                                                                                                                                                                                     tttAGCGAAAAACGGGCGGGAAGTGAAGGTGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAAtt                    <  1:8494/239‑1 (MQ=255)
                                                                                                                                                                                                                               aaggggggggaagggaaGTGGGTTTTGATTTCAGCCGCCGTAATAGCACGGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATTCGGGGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGa          <  1:526609/232‑1 (MQ=255)
                                                                                                                                                                                                                                aCTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCTg                                                                                                           >  1:1077618/1‑141 (MQ=255)
                                                                                                                                                                                                                                  tGGGGGAAAGTGAAGATGTGTTTAATTCCAGCCGCCGTAATTGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAAGCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTc       <  1:51767/239‑1 (MQ=255)
                                                                                                                                                                                                                                     ggggagttGAAGATGGTTTTGATTGCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCGGTGTAGTTCATCATTGCGGGCATGGGGGGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTg     <  1:55333/231‑1 (MQ=255)
                                                                                                                                                                                                                                        gAAGGGAAGTTGGTTTGGATTTCAGCCGCCGTAATTGCCCTGGGCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTgggg  <  1:756219/238‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
TATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGGAGTCTGGGG  >  NC_000913/2974859‑2975326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: