Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,452,852 A→G 30.3% G94G (GGA→GGG)  ytfT → putative sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,452,8520AG30.3% 46.1 / 2.3 30G94G (GGA→GGG) ytfTputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (6/14);  new base G (6/4);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAG  >  NC_000913/4452639‑4453085
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
gCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑cg                                                                                                                                                                                                                   <  1:732110/239‑1 (MQ=255)
gCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑cg                                                                                                                                                                                                                   <  1:164789/239‑1 (MQ=255)
                        gctgctGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGGTGGGTATTTCCCGGACGCCAACGGCTTCGGATA‑CGGT‑CGCCGGGCTAAGCCTGCCGGttgtgt                                                                                                                                                                                           >  1:296375/1‑237 (MQ=255)
                        gctgctGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAAGGATATCGCCGGAGCCACAACGGCTGCGGGTA‑CGGT‑CGCGGGGTTAGGCGTGCCGAtttttt                                                                                                                                                                                           >  1:983888/1‑239 (MQ=255)
                        gctgctGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGGGATGGCCATCGCCGGGGCCCCAACGGCTCCGGTGA‑CGGT‑CGCGGGGTTCCGCCTGCCCAttgttt                                                                                                                                                                                           >  1:486295/1‑239 (MQ=255)
                                         tGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTCGGATGA‑CGGT‑CCCGGGGTTTAGCCTGCCGGTTGTTTTGTTTAGGGCCCTggg                                                                                                                                                                           >  1:830271/1‑238 (MQ=255)
                                         tGGTAGCCCCGCATTTCTGGCAGGAGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGGGGGGGTGGCTATTGCCGGAGCCCAAACAGCTTGGCGTA‑CGGTGCCGGGGATTC‑GCCTGCCCATTGTTTTGTTTAGCGccacggg                                                                                                                                                                           >  1:998862/1‑233 (MQ=255)
                                              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCGTTCGCCGGAGCCACAACGGCCGCGATGA‑CGGT‑GCCGGGGTTTACGCTCCCGATTGTTTTGTTAACAGCCCTGGGGAccc                                                                                                                                                                      >  1:880005/1‑237 (MQ=255)
                                                cccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGGGCCACAAAGGCCGCGGTTA‑CGGT‑CGCGGGGTTCACCCTGCCGGATGTTTTGTTAAGCGCCCTGGGCACCGGc                                                                                                                                                                    >  1:1002792/1‑238 (MQ=255)
                                                 ccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGGGGCCCAACGGCTGCGGTGA‑CGGT‑CGCGGGGTTCAGCCTGCCGCTTGTTTTGTTTACTGCCCCGGGGACCGGcc                                                                                                                                                                   >  1:14957/1‑237 (MQ=255)
                                                  cGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCGCCGTAGGGGCGGGGATGGCTTTCGCCGGGGCCCCAACGGGTGGCAGGAGGGGG‑CGGGGGATCACGGCGCCCGCTTGTTTTGTTAACCGCCCTGGCCCCCGGccc                                                                                                                                                                  >  2:882603/1‑237 (MQ=255)
                                                         ttGGCGGGGGGTTCTTCGGGTGTGGGGTTTG‑TTCGGTCGCCCCATAGACATTTTTAACCGGGCAGCCCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCgg                                                                                                                                                          <  2:486295/238‑1 (MQ=255)
                                                                 tggtgTCTCGGGGTGGGGGTTTT‑GTTGGTGGCCCTATAGACATTCGTGACCGGGCCGCCCTCGTTGGTGTACTGGCGATTGGCATGACGTTGGGGATCGCGCCCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGGGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATtgtg                                                                                                                                                   <  2:14957/238‑1 (MQ=255)
                                                                    gtgtttcggctGGGGGTTTGTTTCGGTGGCCCCATGCGAATGCTTAACCGGGCCGCCCCCGTGGCGTTACGGGCAATTGGCATGACGCTGGTGATCCCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGaa                                                                                                                                                <  2:806899/229‑1 (MQ=255)
                                                                        ttaGGATGGGCGTTGG‑TTCGGTAGCCCCATAGACATTCTTAACCGGGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGGGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCa                                                                                                                                           <  1:240455/237‑1 (MQ=255)
                                                                                                           cttcttcCCCGTGCCCCCCCCGTTGCGTTACTGGCGGTTGGCAGGCGGCTGGTGAGCGCCGCCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGt                                                                                                         <  2:618828/238‑1 (MQ=255)
                                                                                                             tCTTAGCCTTGCTGCTCCGGTTGCGTTACTGGAGTTTGGCATGGCGCTGGTGATCGCCTCCGGTGGGATTGATCTCTCCGTTGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCg                                                                                                       <  1:107388/239‑1 (MQ=255)
                                                                                                              cTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGc                                                                                                      <  1:523193/239‑1 (MQ=255)
                                                                                                                tAACCGTGGAGCTCCCGTTGGGTTACTGGCGATTGGCCTGGCGCTGGTGATCGCCCCCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCa                                                                                                    <  1:1300438/239‑1 (MQ=255)
                                                                                                                                                 tgggggggcGCTGGTGAGCGCTACGGGGGGGATTGTTCTCCCCGGCGGGGCGGGGATGGCTATCGCCGTGGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTgg                                                                  <  2:969109/231‑1 (MQ=255)
                                                                                                                                                   gcctggcgcgGGTGTTCCCCACCGGTGGGGTTGACCTCTCCGGTGGGGCGGTGATGGCTACCGCCGGAGCCACAACGGCTGGGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGgcg                                                                <  2:834312/233‑1 (MQ=255)
                                                                                                                                                       gcggCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTAGCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCaa                                                             <  1:866157/235‑1 (MQ=255)
                                                                                                                                                                             gggATTGATCTCTCCGTAGGGGCGGAGCAGGCTATCGCCCGGACCCCAACGGCCGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGGTTGTTTTGTTAAGCGCCCCGGGCACCGGCATCCTGGCGGGGGTGTGGGAAGGGAGACTGGTAGGGAGACCCAAAATTTAGCCGTTTGTCGCCCCTCCTGTTCTGATGTGGCGCCGGG‑GCGGGGGGGGGGCACGCGTCACCGCCggggcagggg                                       >  2:911769/1‑231 (MQ=255)
                                                                                                                                                                                                 gggggggagggCTATCGCGGGAGCCACAACGGTTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAt                   <  1:804363/229‑1 (MQ=255)
                                                                                                                                                                                                  ggggggATGGCTAGCGGCGGGGCCCAAACGGCTGGGATGA‑CGGT‑CGGGGGATTCAGCCTGGCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtc                  <  1:1254938/233‑1 (MQ=255)
                                                                                                                                                                                                            gctccGCCGGGGCCACAACGGGTGGGGTGA‑CGGT‑CGGGGGATTACGCCTGCCGGTTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGtt        <  2:880005/234‑1 (MQ=255)
                                                                                                                                                                                                                cGCCGGAGCCACAACGGCTGGGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCa   <  1:1263133/239‑1 (MQ=255)
                                                                                                                                                                                                                cGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCCCCGCCGGGCAGACCGTCCCGTTTAACCCGCCGGATTCTTCATGGTTCgccc   >  1:767622/1‑236 (MQ=255)
                                                                                                                                                                                                                cGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCa   >  1:887306/1‑239 (MQ=255)
                                                                                                                                                                                                                 gacGGAGCCACACCGGTTGCGATGA‑CGGC‑CGCGGGATTCAGCCTGCCGATTGTCTTGTTAATCGTCCTTGGCACCGGCATCCGGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAg  <  1:25929/237‑1 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
GCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTG‑TTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGA‑CGGT‑CGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGGGCACCGGCATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATG‑GTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAG  >  NC_000913/4452639‑4453085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑