Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,606,902 A→G 20.2% S267P (TCC→CCC)  rsmC ← 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,9020AG20.2% 152.5 / 6.9 72S267P (TCC→CCC) rsmC16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (9/46);  new base G (14/2);  total (23/49)
Fisher's exact test for biased strand distribution p-value = 2.95e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAATTAACCTTTCTTCGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTT  >  NC_000913/4606664‑4607125
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     
gaTAATTAACCTTTCTTCGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGAAACGGCGGGGTGga                                                                                                                                                                                                                                       >  1:1098056/1‑239 (MQ=255)
 aTAATTAACCTTCCTTCCCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAACTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGgag                                                                                                                                                                                                                                      <  1:497418/239‑1 (MQ=255)
  ttaTTAACCTTTCTTCGCCTGGCGGGTTATAGTGGCGCGATTCCCCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCAAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGgag                                                                                                                                                                                                                                      <  1:1307763/236‑1 (MQ=255)
  tAATTAACCTTTCTTCGCCTGGCGGGTCAAAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGgag                                                                                                                                                                                                                                      <  1:408062/238‑1 (MQ=255)
      tAACCTTTCTTCGCCTGGCGGGTCATAATGGCGCGATTCACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAgatgat                                                                                                                                                                                                                                 <  1:1170134/239‑1 (MQ=255)
         ccTTTCCTCGCCTGGCGGGTCATAATGGCGCGATACTCCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCat                                                                                                                                                                                                                              <  1:195215/239‑1 (MQ=255)
             tcttcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGaatgagccaataa                                                                                                                                                                                                                          >  1:1299561/1‑231 (MQ=255)
             tcttcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGGTGATCagattc                                                                                                                                                                                                                          >  1:192243/1‑237 (MQ=255)
             tcttcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGAGGTGGGCATATCa                                                                                                                                                                                                                          >  1:768758/1‑239 (MQ=255)
             tcttcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGAGGGGATcctgata                                                                                                                                                                                                                          >  1:821358/1‑233 (MQ=255)
             tcttcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCACCGTGGAACGGGGGGTTGGGGATGTGCATATcc                                                                                                                                                                                                                          >  1:804831/1‑238 (MQ=255)
                tcGCCTTGGGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTTTTTGCCGGATCCCTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCaaaa                                                                                                                                                                                                                       <  1:334343/239‑1 (MQ=255)
                gcGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCGGTTTGCGCGATCACTTCGTGGAAGCAAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCACCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCaaaa                                                                                                                                                                                                                       <  1:852616/238‑1 (MQ=255)
                      ggCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCACCGTTTGCGCCGCATCCAGGCTGGTTTCCATCCCATCGTGGAACGGCGGGTTGGGGAGGTTCTTTTCAAAACgcc                                                                                                                                                                                                                   >  1:263536/1‑235 (MQ=255)
                              aTAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGAGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTc                                                                                                                                                                                                       <  1:438609/239‑1 (MQ=255)
                                 aTGGCGCGATACACCTTGAAGCCGCCGGGTTGCGCGATCACTTCGTGGAAGCCAAATGTTTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGa                                                                                                                                                                                                    <  1:196069/239‑1 (MQ=255)
                                     cgcgATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGCCGGGTTGTGGAGGCACATATTAAAACAACCTTTCACCTCCGaaaaa                                                                                                                                                                                                >  1:357124/1‑238 (MQ=255)
                                      cggattccccTTTAAGCGCCCTGGTTGGGCGCTCCCTTGGGGGAAGCCAAATGGCCCATCCAACCCGTCCGGATAAGGCAGGAAGGCGTTCGCTAAAATTCGCGGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAg                                                                                                                                                                                                <  2:41070/230‑1 (MQ=255)
                                       cgATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTTACCTCGGAAAAAAc                                                                                                                                                                                              >  1:41070/1‑239 (MQ=255)
                                           cccccTTGAAGCGCCCTGTTTGGGCGATCCATTGGGGGAATCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTg                                                                                                                                                                                         <  2:787645/236‑1 (MQ=255)
                                               cTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGGGATGGTCATATACAAACGGCCTTTACCCCCGGAAAAAAGATTGCTgg                                                                                                                                                                                     >  1:384722/1‑239 (MQ=255)
                                                 tgcaGCCCCCTTTTTGTCTGATCCCTTCTGGGAGCCAAAATGCCTCCCCCACCCCGCCCGGAGAAGGCAGGAAGGCCTTCGCTACAATTCGCCGCTCGCCGCCGCTATTAAGATGTCGCACCGCACCTCGAATCAGCGTTTGCGCCGCATCCATGCTGGTTTGCATCCCATCGTGGAACGGCAGGGTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCa                                                                                                                                                                                   <  2:199736/236‑1 (MQ=255)
                                                 tGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGGTGATCATATCTAAACGCCCCTTCCACTCGGGAAAAACGGTTCTGGCa                                                                                                                                                                                   >  1:663098/1‑239 (MQ=255)
                                                   caGCGCCCTTTTTGCGCGATCCCTTCGGGAAAGCCAAATGTCTCTCCCAGCCCGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCaaa                                                                                                                                                                                 <  2:804831/238‑1 (MQ=255)
                                                   aaGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGGCGGGCATATCAAAACGCCCCTTTCCCCCGGAAAAAACGTTCGCTGGCaa                                                                                                                                                                                  >  1:1198853/1‑239 (MQ=255)
                                                    aGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCATATCAAAAACACCTTTCACCTCGGAAAAAACGTTGCTGGCCAAg                                                                                                                                                                                >  1:1119051/1‑239 (MQ=255)
                                                    aGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGAGACGGTCATATCAAAACGACCTTTCACCTCGGAAAAAACGGTGCCTGCaaa                                                                                                                                                                                 >  1:287067/1‑238 (MQ=255)
                                                      cgcCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCTTGGAACGGCGGGGTGGGGGAGATCCTATTCAAACGCCCCTTTCCCCCGGGAAAAAAGGttccgtcaacaaa                                                                                                                                                                                >  1:849918/1‑228 (MQ=255)
                                                        cccTGTTTGCGCGATCCCTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACtt                                                                                                                                                                            <  1:736841/239‑1 (MQ=255)
                                                        cccTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTTAACTCCGAAAAAAAGATGCTGGCAAAAACt                                                                                                                                                                             >  1:1019072/1‑238 (MQ=255)
                                                         cttttttGCGCGATCTCTTCGTGGAAGCCAAAGGTCTCATCCAGCACGGCCGGAGAAGGCAGGAAGGGGTGCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTc                                                                                                                                                                           <  2:1204967/235‑1 (MQ=255)
                                                         ccggTTTGCGCGATCCCTTCGTGGAAGCCAAATTGCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTc                                                                                                                                                                           <  1:1298605/236‑1 (MQ=255)
                                                         ccTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGCTGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGAGGATCATATTCAAACCACCTTTCACCTCGGGAAAAACCTTGCTgtgcacaacat                                                                                                                                                                            >  1:291824/1‑228 (MQ=255)
                                                         ccTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCATATCAAAAAGACCTTTTACCCCGGGAAAAACGTTGCTGGCAAAAAcat                                                                                                                                                                            >  1:47851/1‑236 (MQ=255)
                                                         ccTGTTTGCGCGAGCACCTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGTTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCAACGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTc                                                                                                                                                                           <  1:818996/239‑1 (MQ=255)
                                                                gcggGATCCTTTCTTGTAAGCAAAATTTCTCCGCCAGCCCGTCCGGATAAGGCAGGAAGGGGGTCGCTACAATTCGCAGGTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCa                                                                                                                                                                    <  2:663098/239‑1 (MQ=255)
                                                                 cgcaATCCCTTCGTGTAAGCCAAAGTTCCCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTCCAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCAcc                                                                                                                                                                                                          <  1:387998/202‑1 (MQ=255)
                                                                  gcgATCCCTTGGGGGAAGCAAAATGGCCCACCCACCCCGTCCGGAAAAGGCAGGAAGGCGTTCGCTACAATTCGCCGCCTGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAac                                                                                                                                                                  <  2:1127016/239‑1 (MQ=255)
                                                                      tCACTTCGGGGAAGCCAAATGTCTCATCCGGGCCGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCg                                                                                                                                                             <  1:562136/239‑1 (MQ=255)
                                                                         ctgcGTGGAAGCCAAATGTCTGATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTg                                                                                                                                                          <  1:927972/236‑1 (MQ=255)
                                                                         cTTCGTGGAAGCCAAATTTCTTCTCCAGCACGGCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTg                                                                                                                                                          <  1:242947/239‑1 (MQ=255)
                                                                               ggAAGCCAAATTTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCCCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGc                                                                                                                                                     <  1:1119743/238‑1 (MQ=255)
                                                                                             ctcATCCAGCGCGTCCGGAAAAGGCAGGAAGGCGTTCGCTACAATGCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCgg                                                                                                                                        <  1:1362704/238‑1 (MQ=255)
                                                                                             ctcATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTAAAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCgg                                                                                                                                        <  1:1156429/238‑1 (MQ=255)
                                                                                                         gTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTAccg                                                                                                                           <  1:1326571/239‑1 (MQ=255)
                                                                                                           cctgATAAGGCAGGAGGTGTTTCGTTAATATTCGCAGTTCGCCGCCGGTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAACCGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTAccgcc                                                                                                                         <  2:534849/236‑1 (MQ=255)
                                                                                                            cGGATAGGGCGGGAAGGGGTTAGCTTCACTGCCCAGTCTCCCCCCGCTATTAAGATGACCACCCGCCCCGGGAATCAGGGTTTGGGCCGCTTCCAGGCTGGTTTGCATTCCATCGTGGAAAGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTAccgcc                                                                                                                         <  2:287067/238‑1 (MQ=255)
                                                                                                            cGGATAAGGCAGGAAGGCGTTCCGTACAATTCGCAGCTCGCCGCCGGTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTAccgcc                                                                                                                         <  1:32040/238‑1 (MQ=255)
                                                                                                                   ggCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGATCATATCAAAACGCCCTTTTCCCCCGGAAACGACGTCGCTGGCAAAGACTTCCCCTTCACACACGTTTGCCGCACGAGTTGCGGCGCTGGCCTCTACCGCCgggggcag                                                                                                                 >  1:1289063/1‑235 (MQ=255)
                                                                                                                   ggCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTTGGGTTGTGAATATCAAAAACACCCTTCCCCTCCGGAAAAGCGGTGTTGGCAAAGACCTTCCCTTAACAACCCTTTGCCGCCACAGGTGCGCCGGCGGCTCCTCCCCCCCGGGcgaa                                                                                                                 >  2:362734/1‑236 (MQ=255)
                                                                                                                       ggAGGGCTTTCGCTAAAATGCGCATCTCGCCGCCGTTATTAAGATGACGCGCCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGAc                                                                                                              <  2:121922/238‑1 (MQ=255)
                                                                                                                         aaGGCGTTCGTTACAATTCGCAGCCCGCCGCCGCTATTAAGATGACGCCCCGCACCGCGAATCAGCGTTTGCCCCGCATCCAGGCTTGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGGCCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACa                                                                                                             <  1:1326111/237‑1 (MQ=255)
                                                                                                                         aaGGCGTTCGTTAAAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACAt                                                                                                            <  1:538540/238‑1 (MQ=255)
                                                                                                                                 cGCTCCAATCCGCACCTCCCCGCCGCTATTAAGATTACGCACCGCACCGCGAATCAGCTTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAgg                                                                                                   <  1:1264301/239‑1 (MQ=255)
                                                                                                                                  cccaCAATTCGCGGCCCGCCCCCGCTATTAAGATGCCGCCCCCCACCGGGCATCAGCGTTTGCGGCGCATCCAGGCTGGTTTGCATCCCAGCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGt                                                                                                  <  2:1289063/236‑1 (MQ=255)
                                                                                                                                                                      aCGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCCCAAGGGGAGACGGATTTTCGGCGAAGGGGCCGCAAAggga                                                              >  1:1199815/1‑236 (MQ=255)
                                                                                                                                                                      aCGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGGTGGAGATGATCATATCAAAACGACCTTTCAACTCGGAAAAAACGTTGCTGGCAAAGACTTTCCCCTCAACACCGTTGGCCGCAAGGGTTTGCCGGCGGGCTTCTCCCCCCGGGGCCGGGGAAACCCACAAGGGGGGGCGAAATTTATGGGAAATGGCGGCAAAAGCaa                                                             >  2:872073/1‑239 (MQ=255)
                                                                                                                                                                             gccccgcGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAg                                                      <  1:362734/236‑1 (MQ=255)
                                                                                                                                                                             gcaccggGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAg                                                      <  1:210145/239‑1 (MQ=255)
                                                                                                                                                                                   ggAATCCGCGTTTGGGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCGCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGcc                                                <  1:255266/238‑1 (MQ=255)
                                                                                                                                                                                     aaTCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTgaga                                                                                              <  1:966833/193‑1 (MQ=255)
                                                                                                                                                                                               tGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGGGATGGTCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTTACCTTCAACACCGTTGGCCGCAAGGGTTGCGCGGCTGGCCTCTACCGCCGGGGGAGAGACATCCCCCAAGGTGAGACGAATTTTCg                                                                                   >  2:966833/1‑193 (MQ=255)
                                                                                                                                                                                                tccccccagccAGGCGTGTTTGCCACCCCGCGGTGAACGGCGGTTGGGAGATGAACATACCAAACCCGTGTTTCACCTCGGAAAGGACGTTGCTGGCAAAGGCTTCCCCTTCAACCCCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCg                                   <  2:1098056/230‑1 (MQ=255)
                                                                                                                                                                                                    cagaTCCAGGCTGGTTTGCAACCCATCGGGGAACGGCGGGTTGGAGATGAACATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACa                                <  1:462033/235‑1 (MQ=255)
                                                                                                                                                                                                     gcATCCCGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTGCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATc                              <  1:1157021/239‑1 (MQ=255)
                                                                                                                                                                                                      cctCCAGGGTGTTTGTCTTCCCATCGTGGAACGGGGGGTTGGGGATGATCATACAAAAACGACCTTTCCCCGCGGAAAAGACGTTGCTGGCAAAGCCTTCCCCAGCAACCCCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATcc                             <  2:34802/237‑1 (MQ=255)
                                                                                                                                                                                                      atctcAGGCTGTTTTGCACCCCATCGGGGAACGGGGTGTTGGACATGATCATCTCAAAACGACCTTTGACCACGAAAAAGACGTTGCTGGCAAAGCGTTCACCGTCAACGCCGTTGGCCGCAATTGTGTGGCGGCTGGCTTCTGCCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTCCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATcc                             <  2:357124/235‑1 (MQ=255)
                                                                                                                                                                                                       ctCCAGGTTTGTTTTCCTCCCATCGTGGAACGGCGGGTTGGCAATGATCATATCAAAACGCCCTTTCCCCTCGGAAAAGGCGTTGCTGGCAAAGCCTTCACCTTCAACACCGTTGGCCGCAAGTTTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCa                            <  2:1299561/238‑1 (MQ=255)
                                                                                                                                                                                                       aTCCAGGCTGTTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGGCCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCa                            <  1:1183053/239‑1 (MQ=255)
                                                                                                                                                                                                       aTCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCCCCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCa                            <  1:424039/239‑1 (MQ=255)
                                                                                                                                                                                                                     gCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCCCCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTc              <  1:1160721/239‑1 (MQ=255)
                                                                                                                                                                                                                           cATCGTGGAACGGGGGGTTGGGGATGTTCATATCAAAACCACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGAAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGc        <  1:250962/239‑1 (MQ=255)
                                                                                                                                                                                                                           cATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGc        <  1:1082118/239‑1 (MQ=255)
                                                                                                                                                                                                                           cATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCCGAAAAGACGTTGCTGGCAAAGACATCAACTTCAACACCCTTTGCCGCCAGAGTTGCGCGGCTGGCTTTTACCGCCGGGGCAGAAGCATCCCACAAGGGGAGACGAATTTTTGACGCAAGACGCGCAAAGAGCAACACAAAACACCCACGCCCCAACCCCACCACCAACACTTTAACCTTctaggcg        >  2:456929/1‑233 (MQ=255)
                                                                                                                                                                                                                           cATCGTGGAACGGCGGGTTGGAGATGATCATAACAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTGGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGc        <  1:879161/239‑1 (MQ=255)
                                                                                                                                                                                                                                  gAACGGGGGGTTGGAGATGATCAGATCAAACCGGCCTTTCCCCTCGGAAAAGGCGTTGCTGGCAAAGCCTTCACCTTAAACACGTTTGCCCGAAAGTGTTGCGCGGCTGGCTTCTCCCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGtt  <  2:849771/238‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     
GATAATTAACCTTTCTTCGCCTGGCGGGTCATAATGGCGCGATACACCTTGAAGCGCCCTGTTTGCGCGATCACTTCGTGGAAGCCAAATGTCTCATCCAGCACGTCCGGATAAGGCAGGAAGGCGTTCGCTACAATTCGCAGCTCGCCGCCGCTATTAAGATGACGCACCGCACCGCGAATCAGCGTTTGCGCCGCATCCAGGCTGGTTTGCATCCCATCGTGGAACGGCGGGTTGGAGATGATCATATCAAAACGACCTTTCACCTCGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTT  >  NC_000913/4606664‑4607125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: