Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	910,397	T→C	13.6%	E218G (GAG→GGG)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,397	0	T	C	13.6%	241.6 / 3.6	93	E218G (GAG→GGG)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base T (48/28);  new base C (2/12);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 9.30e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTA  >  NC_000913/910163‑910631                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            gctggGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCTTGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AATTCttt                                                                                                                                                                                                                                         >  1:128399/1‑239 (MQ=255)    gggTTGATATCCATCCGAATGATTTTGGCATCGGTCCGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATg                                                                                                                                                                                                                                      <  1:1038079/239‑1 (MQ=255)              tattCTGAATTATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATGGCGTGCCGGGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCcgcg                                                                                                                                                                                                                           <  2:288788/236‑1 (MQ=255)              cAATCTGAATGATTTTGGCATCGGGCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCcgcg                                                                                                                                                                                                                           <  1:1251072/239‑1 (MQ=255)               aaTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCgcgc                                                                                                                                                                                                                          >  1:922724/1‑239 (MQ=255)                aTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGGGAACCCGATTAACCGGGCCATTCCAACTTCAGAGCGATTACCGTATTCGACTTGTTCTTTCCCGCCAAGGGCTAGAACAATAGGGCCTTTAATTTTCCCGG‑AAAGACACACA‑AACTCTTTTTGACCCCCCcgcc                                                                                                                                                                                                                           >  2:285631/1‑237 (MQ=255)                  cTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGc                                                                                                                                                                                                                       <  1:121027/239‑1 (MQ=255)                    gAATGATTTTGGGATCGGTCGGGTAGAAGGCTCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCg                                                                                                                                                                                                                     <  1:446844/239‑1 (MQ=255)                    gAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCg                                                                                                                                                                                                                     <  1:972110/239‑1 (MQ=255)                     atggtttttGGCATGGGTGGGGTAGAAGGGGCGGTGGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACAGGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGc                                                                                                                                                                                                                    <  2:316147/234‑1 (MQ=255)                     aaTGATTTTGGCATCGGTCGGGTAGGAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGcc                                                                                                                                                                                                                      >  1:762952/1‑237 (MQ=255)                      atgtgtgtGGCATGGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGc                                                                                                                                                                                                                    <  1:888774/231‑1 (MQ=255)                       tGATTTTGGAATCGGTGGGGTAGAAGGGGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTGCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTg                                                                                                                                                                                                                  <  1:683356/239‑1 (MQ=255)                               gCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CCCCCCCCCCCCACCGCTGCcacaacc                                                                                                                                                                                                          >  2:498659/1‑236 (MQ=255)                                               aGGCCCTGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGc                                                                                                                                                                                            <  1:1131147/237‑1 (MQ=255)                                                ggCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCCCTCCCACACCTCAGGGGGAAATTGCTgg                                                                                                                                                                                         >  2:1070000/1‑239 (MQ=255)                                                    cgGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAggg                                                                                                                                                                                                     >  1:667521/1‑223 (MQ=255)                                                    cgGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATa                                                                                                                                                                                     >  1:157758/1‑239 (MQ=255)                                                    cgGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATa                                                                                                                                                                                     >  1:1047465/1‑239 (MQ=255)                                                      gTAGGGAAATTGGGTGCCGAGTAGCACTAACGTGTCGGCGTTTATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATTCGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATaa                                                                                                                                                                                    <  1:498659/238‑1 (MQ=255)                                                        aGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTTCTGGATTAACg                                                                                                                                                                                  >  1:1081422/1‑238 (MQ=255)                                                                 gCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACAAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTCTCGTATTCGACATGTTCTTTACCGGGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACCCC‑ACT‑ACCCCATTTGC‑CGCCCCCGCGCACCCGCTGCCACACATCAGGGGGATTATGCGGGAATAACCCACAAGTTgg                                                                                                                                                                        >  2:1371180/1‑238 (MQ=255)                                                                         aGTAGCACTAACGTGTCGGCATTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCCCTGCCACACATCAAGGCGAAATTTCTGGAAAAACCCAGCAGTTGCGCCAGttt                                                                                                                                                                >  2:131604/1‑239 (MQ=255)                                                                             gCACTAACGTGTCGGCGTTTACCATGGTAAGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGt                                                                                                                                                            <  2:762952/239‑1 (MQ=255)                                                                                   aCGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAActct                                                                                                                                                      >  1:385054/1‑239 (MQ=255)                                                                                   aCGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAActct                                                                                                                                                      >  1:748895/1‑239 (MQ=255)                                                                                   aCGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAActct                                                                                                                                                      >  1:1284895/1‑239 (MQ=255)                                                                                   aCGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCAAACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAActct                                                                                                                                                      >  1:369155/1‑239 (MQ=255)                                                                                    cGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGCCGCTATTGCTGGTATACCGCAGCAGTTCCCCCAGTTTCCGTAACtctt                                                                                                                                                     >  1:1252760/1‑239 (MQ=255)                                                                                        tCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGAGCAGGGCATGAACAATAGGCGCTTTAATATTCCCGGCAACCTCA‑ACT‑AACCCTTTATG‑AGCCCCCGCGCAGCCGCGGCCCAACACCAGGGCGAAATTCCTGGAAAAACGCAGACGTTGCGCCAGTTTGGGGAACccttcttt                                                                                                                                                 >  2:1026187/1‑238 (MQ=255)                                                                                           gCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTcttcttctt                                                                                                                                               >  1:1068412/1‑238 (MQ=255)                                                                                                catcatGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACCTCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCGGTTTGCGTAACTCTTCTTCTTCCGGc                                                                                                                                          >  1:277149/1‑238 (MQ=255)                                                                                                  tcatGTTTTGGAAACCTTACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTCCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACCCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTc                                                                                                                                       <  2:1284895/239‑1 (MQ=255)                                                                                                       ttATTGAAACCCTACGAGAAGCCGATTAATCCGGTCATTCCAACATCATACGGATTATCGTTTTCGACATGTTCGTTCCCGCGCAGGGGATGAAAAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGAc                                                                                                                                  <  2:1270979/238‑1 (MQ=255)                                                                                                       gTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGc                                                                                                                                                                                                                    <  1:825173/157‑1 (MQ=255)                                                                                                       gTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGc                                                                                                                                                                                                                    >  2:825173/1‑157 (MQ=255)                                                                                                                ccTGACGAGAAGGCGATTAACCCGGTTATTCCAACATCATGCGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTgg                                                                                                                         <  1:363735/239‑1 (MQ=255)                                                                                                                  tGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACCCTTCTTATTCCGGCGTCACGACTGGTTGTGgc                                                                                                                        >  1:1313816/1‑238 (MQ=255)                                                                                                                            cctaTTACCCCTGTCATTCCACCAGTGTGCGGATTATCGTTATCTACATGTTCTTGTCCTGGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACCCTTTAAG‑CGCCCCCGCGCAGCCGCTGCCACACAGCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATAc                                                                                                               <  2:128399/234‑1 (MQ=255)                                                                                                                                             tCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCtt                                                                                            <  1:131604/239‑1 (MQ=255)                                                                                                                                                  catcatACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGCACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGt                                                                                                                                                                  >  2:1370479/1‑164 (MQ=255)                                                                                                                                                  catcatACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGt                                                                                                                                                                  <  1:1370479/164‑1 (MQ=255)                                                                                                                                                   atcatACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATTGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGgcg                                                                                      <  1:220710/239‑1 (MQ=255)                                                                                                                                                       tACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAgg                                                                                  >  2:858421/1‑239 (MQ=255)                                                                                                                                                       tACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAgg                                                                                  >  1:159495/1‑239 (MQ=255)                                                                                                                                                       tACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGCTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACAAGTGCATGGTGGCCCCtccggcggcggg                                                                                  >  2:867968/1‑228 (MQ=255)                                                                                                                                                          ggATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCATAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGttt                                                                               <  1:1070000/239‑1 (MQ=255)                                                                                                                                                            aTTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCCGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACCGGTTGTGCCGCATGATACCATGGCATGGTTGCCCCTTCTGGCGCAGGGTTTa                                                                             >  2:801839/1‑239 (MQ=255)                                                                                                                                                            aTTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGGTTTa                                                                             >  1:242822/1‑239 (MQ=255)                                                                                                                                                              tATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTaa                                                                            >  1:410010/1‑238 (MQ=255)                                                                                                                                                               aTCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGCTATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCCCGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAc                                                                           >  1:643294/1‑238 (MQ=255)                                                                                                                                                               aTCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGGGCATGATACCAGTGAATGGTTGCCCCTTCTGGGCCAGGTTTTaaa                                                                           >  2:974272/1‑237 (MQ=255)                                                                                                                                                                  gTATTCACCTTGTTTTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTGCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGGAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCTGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCa                                                                       <  2:541670/239‑1 (MQ=255)                                                                                                                                                                    tttCCACATGTTCTTTACCCCGCAGGGAATGAACAAGTGGCCCTTTAAGTTTCCCGGCAAACCCA‑ACT‑AACCCTTTTTG‑CGCCCCCCCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACg                                                                     <  2:1081422/238‑1 (MQ=255)                                                                                                                                                                     ttCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCcaca                                                                                                                                                                                                             <  2:825105/102‑1 (MQ=255)                                                                                                                                                                     ttCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCcaca                                                                                                                                                                                                             >  1:825105/1‑102 (MQ=255)                                                                                                                                                                          cATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTgg                                                                                                                         <  1:432479/181‑1 (MQ=255)                                                                                                                                                                          cATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTgg                                                                                                                         >  2:432479/1‑181 (MQ=255)                                                                                                                                                                              ttCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCCCAGGTTTTAACGCCACGTCGCCTGGt                                                            >  1:572174/1‑238 (MQ=255)                                                                                                                                                                                   tACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGCTATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCATGTTTTAACGCCACGTCGCCTGGTAccac                                                       >  1:1230827/1‑238 (MQ=255)                                                                                                                                                                                   tACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGTCGTCACGACTGGTTGTGGCGCATGATACCCGTGCATGGTTGCCCCTTCTGGCGCCGGTTTTAACGCCACGTCGCCGGGGaaacag                                                      >  2:894660/1‑235 (MQ=255)                                                                                                                                                                                      cgcgCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAGGCCACGTCGCCTGGTAACACGCCa                                                   >  1:666669/1‑239 (MQ=255)                                                                                                                                                                                        cgcAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGGTTTAACGCCACGTCCCCTGGaaaacacaaaa                                                  >  2:446494/1‑230 (MQ=255)                                                                                                                                                                                        cgcAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGGGCATGGTTGCCCCTTCTGGCGCTGGTTTTAACGCCACGACCCCTGGTAACAAGACaa                                                  >  2:1042758/1‑238 (MQ=255)                                                                                                                                                                                        cgcAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTCGTTGTGGCGCATGATACCATTGCATGTTTGCCCCTTCTGGCGAAGGTTTTAAAGCCCCGACCCCTGGGAAAACgcaaa                                                  >  2:372520/1‑234 (MQ=255)                                                                                                                                                                                           ctggCATTAACAAGAGGCGTTTTAATTTCCCCGGCAAACTCA‑AAT‑AGCTCTTTATG‑CGCCCCCGCGGCGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGa                                              <  2:277149/237‑1 (MQ=255)                                                                                                                                                                                           aGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAc                                                                           >  2:972032/1‑210 (MQ=255)                                                                                                                                                                                           aGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAc                                                                           <  1:972032/210‑1 (MQ=255)                                                                                                                                                                                              gtaTGAACAATGGGCCCTTTAAGTTTCCCAGCAAACCCA‑ACC‑AACCTTTTATG‑CTCCCCCGCGCAGCCGCTGCCACCCATCAGGGCGGTATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGa                                              <  2:369155/234‑1 (MQ=255)                                                                                                                                                                                              gCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACGCTTTATG‑CGCCCCCGCGCAGCCGCTGCCAAACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGaaa                                            <  2:922724/238‑1 (MQ=255)                                                                                                                                                                                                aTGAACAATAGGCGCTTTAATTTTCCCGGCAATCTCA‑ACT‑ACCCCTTTATG‑CCCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGc                                         <  1:1026187/239‑1 (MQ=255)                                                                                                                                                                                                aTGAACAAGTGGCTCGTGAATTGTCCCGGCAAACCCA‑ACT‑AGCCCTGGAGG‑CGCCCCCGCGCGGCCGCTGCCAACCATCAGGGCGATATTGCTGGGAGTACGCAGCAGTTGCGCCAGTTGGCGTAACTCATCTTCTTCCTGCGTTACGACTGGTTGTGGCGCATGATTCCATTGCATGGTTGCCACTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGc                                         <  2:99793/239‑1 (MQ=255)                                                                                                                                                                                                  gaccAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACGCTTTATG‑CGCCCCCGCGCAGCGGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCa                                       <  2:176622/236‑1 (MQ=255)                                                                                                                                                                                                  gAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACGCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCa                                       <  1:858421/239‑1 (MQ=255)                                                                                                                                                                                                  gAAAAAAAGGCCCTTTAATTTTCCCGGCAAACCCA‑ACT‑AACCCTTTTTG‑CCCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCa                                       <  2:748895/239‑1 (MQ=255)                                                                                                                                                                                                       aTAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCCCGACTGGTTGTGGCGCATGCTCCCAGTGCATGGTTGCCCCTTCTGGCGCAGGCTTTAACGCCACGTCGCCTGGTAACACGCACACCGAAACCCCACGGtt                                  >  2:1371652/1‑239 (MQ=255)                                                                                                                                                                                                       aTAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCCCGTCGCCTGGGAACACGACAACCGAAACCCCACGGta                                  >  2:754617/1‑238 (MQ=255)                                                                                                                                                                                                       aTAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGtt                                  >  1:156104/1‑239 (MQ=255)                                                                                                                                                                                                       aTAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAAGCCACATCGCCTGGTAACCCCGCAACCGAAACCCCAAgggt                                  >  2:1343008/1‑237 (MQ=255)                                                                                                                                                                                                        tAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGCTACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCCCCTGGTAAAACACCAACCGAAACGCCACGGTTa                                 >  2:395830/1‑239 (MQ=255)                                                                                                                                                                                                             cttttAATTTTCCCGGAAAACCCA‑ACT‑AACCTTTTATG‑CGCCCCCGCCGAGCCGCGGCCCCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCa                             <  2:157758/236‑1 (MQ=255)                                                                                                                                                                                                             ccTTTTATTTTCCCCGCAAACCCA‑ACT‑ACCCTCTTAAG‑CCCCCCCGCGCCGCCGCTGCCAAACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCa                             <  2:1068412/237‑1 (MQ=255)                                                                                                                                                                                                             ccTTTAATTTTCCCGGCCAAC‑CA‑ACTCAACCCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGGGATATTGCGGGAACAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCa                             <  2:242822/237‑1 (MQ=255)                                                                                                                                                                                                                ttAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCCCGACTGGTTGTGGCGCATGATACCAGTGCTTGGTTGCCCCTTCTGGCGCCGGTTTTAACGCCACGTCCCCTGGTAAAACGACCACCGAAACCCCACGGTTAaccacc                           >  2:277275/1‑237 (MQ=255)                                                                                                                                                                                                                ttAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCGTGATACCAGTGCATGGTTGCCCCTTCTGGCTCAGGTTTTAACGCCCCGTCGCCTTGTAACACGACAACCGAAACGCCACGGATAAGAAcc                           >  2:549492/1‑237 (MQ=255)                                                                                                                                                                                                                ttAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGCCGCCTGGTAACACGACAACCGAAACCCCCAGGTTaaaaacc                           >  2:608324/1‑232 (MQ=255)                                                                                                                                                                                                                 tAATTTTCCCGGAAAACTCA‑ACT‑AACCTTTTATG‑CGCCCCCGCGCAGCCGCTGCCAAACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCt                        <  1:395830/239‑1 (MQ=255)                                                                                                                                                                                                                        cccGGCAAACTCA‑ACT‑TACCTTTTTTG‑CCCCCCCCCGCCGCCGCTGCCAAACATCAGGGCGATATTTCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCa                 <  2:159495/239‑1 (MQ=255)                                                                                                                                                                                                                        cccGGCAAACTCA‑ACT‑AACTCTTTAGG‑CGCCCCCGCGCAGCCGCTGCCCCACATCAGGGGGATATTGCTGGAATAACGCAGCAGTTGGGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCa                 <  1:608324/239‑1 (MQ=255)                                                                                                                                                                                                                            gCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACTGCAACCGAAAGGCCACGGTTAAGCACCGCTTTGCGCCTGGc             >  1:73643/1‑239 (MQ=255)                                                                                                                                                                                                                             cAACCTCA‑ACT‑CACTTTTTATG‑CCCCCCCGCGCAGCCGTGGCCCCACATCAGGGCGATTTTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCa            <  2:666669/239‑1 (MQ=255)                                                                                                                                                                                                                              agaCTAA‑ATT‑AACTCTTTAGG‑CCCCCCCCCGGTGCCGCTGCCAAAGATCAGGGCGATATTGCTGGAATAACGCGGGAGTTGCGCCAGTTTGCGTAACCCTTCTTCTTCCGGCGTCACGTCTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCa            <  2:1252760/236‑1 (MQ=255)                                                                                                                                                                                                                               aaCTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAAt          <  1:1343008/239‑1 (MQ=255)                                                                                                                                                                                                                                    a‑act‑aactCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCa     <  1:446494/239‑1 (MQ=255)                                                                                                                                                                                                                                      att‑aactCTTTTGG‑GCCCCCCGGGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAg    <  1:314779/237‑1 (MQ=255)                                                                                                                                                                                                                                        t‑aactCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTa  <  1:754617/239‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            GCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCA‑ACT‑AACTCTTTATG‑CGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAACGCCACGTCGCCTGGTAACACGACAACCGAAACGCCACGGTTAAGCACCGCTTTGCGCATGGCAATCGCCAGTA  >  NC_000913/910163‑910631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑