Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,030,269	C→T	17.6%	S69F (TCT→TTT)	yccX →	weak acylphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,030,269	0	C	T	17.6%	76.9 / 2.8	54	S69F (TCT→TTT)	yccX	weak acylphosphatase
Reads supporting (aligned to +/- strand):  ref base C (13/30);  new base T (7/2);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 1.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAG  >  NC_000913/1030037‑1030490                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 cgTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGGAGG‑GC‑GGGCCCCGCTTTgcggt                                                                                                                                                                                                                            >  2:333084/1‑237 (MQ=255)        taaggataaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGaa                                                                                                                                                                                                                     <  1:47399/239‑1 (MQ=255)            gataaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCCTGGAGAACcgg                                                                                                                                                                                                                 >  1:894266/1‑239 (MQ=255)               aaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGGTTTGCGGCTGTGGAACggggg                                                                                                                                                                                                               >  1:1123119/1‑236 (MQ=255)                aggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGGAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGGGGGTGTGGAACggggggt                                                                                                                                                                                                             >  1:127419/1‑235 (MQ=255)                     agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAAAGTG‑GC‑GGCCCCCGGTCTCGGGGTGTTGAACgggggggcgggg                                                                                                                                                                                                        >  2:859797/1‑230 (MQ=255)                      gcagcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTCACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATCCAGTGGTTAAAGAGGG‑GC‑GGACAGGGGGATGCGCGCGGGGAAAGGGGGCTAAGCGa                                                                                                                                                                                                       >  2:1183921/1‑239 (MQ=255)                        ataaaTGTCGAAAGTTGGCATAATTCCCGGGGTTTACGGGGGATGTCAGGGCGTAGGATTGCGCTACCGCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAAGGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAg                                                                                                                                                                                                      <  2:563506/235‑1 (MQ=255)                            aTGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGGGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGATTTGCGCGCGTAGAACGGGGGCGACGCGAGCCGc                                                                                                                                                                                                  >  1:845685/1‑238 (MQ=255)                                  aaaGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGGTGTGGGCGGGTAGAACAGGGGGCAAGCGAGACGcgcaacc                                                                                                                                                                                            >  2:421773/1‑232 (MQ=255)                                      tCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCATGGGTTAAAGGGTG‑GCGGGCCCGCGTTTTGGGCGTGTAGACGGGGGGCTAAGAGACGCGCATCAccccgc                                                                                                                                                                                        >  2:340488/1‑237 (MQ=255)                                        ggaaaaaatGCGTGGGTTTAGGGGGGTGTGCGGGGCGTAGGTTTCCGCAACCCCACACAGGACGAAGCGAAAAGACTGGGTTTCACCGGGTACGCCAAAAATTTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGGGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCggg                                                                                                                                                                                     <  2:845685/231‑1 (MQ=255)                                         gCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGTTCTGCCGGGGTGGAACGGGGGTTCCGCGGGCCGCGTCCTCCCCCgggg                                                                                                                                                                                    >  1:475914/1‑239 (MQ=255)                                               ttGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGGCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTa                                                                                                                                                                              <  1:1183921/239‑1 (MQ=255)                                                  ccTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTGGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTTTGCGCGTGTAGAACGGGGGGTCAGCGAGCCCACTCACTCCCCGGGGGGATTTAc                                                                                                                                                                            >  1:906926/1‑238 (MQ=255)                                                    tGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGGCCGTGTAGAACGGGGGCTGAGCGGGCCGGCGCCGCCCACGGGGGGATTAAACgg                                                                                                                                                                         >  1:338548/1‑238 (MQ=255)                                                       gTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTTTGCGCGTGTAGAACGGGGGCTCAGCGAGCCCCACCATCCCTCGGGGGGATTAACCGAttt                                                                                                                                                                      >  1:335284/1‑239 (MQ=255)                                                        tttACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAAc                                                                                                                                                                                                                    <  1:1220194/192‑1 (MQ=255)                                                             gggCGTGTTCGGGGCGAAGGATTTGGCAACCCCCACCAGGCAGAGTCAAAAAGCATGGGTTTCAACGGGTACGCCAAAAATCTTGGCGGTGGAAGCGTGGACGTGGTTGCTTGGGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  2:846193/239‑1 (MQ=255)                                                             gggCGAGGTAAGGGCGTAGGATTTCGCTTCACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGGGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  1:266424/239‑1 (MQ=255)                                                                 ggtttCGGGGCTTGGGGTTTCGCTTCCCCCCAAAGTAAGGAGCGAAAAGACTGGGTTTAACGGGGTCCGCAAAAAATGTTGCAGGTGGCAGGGTGGAAGGGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCg                                                                                                                                                             <  2:245560/235‑1 (MQ=255)                                                                                        tacaccgaccaGTACGGAGCAAAAAGGCTGGGTTTAACCGGGTACGCCAAAAATCTTGACGTTGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTgg                                                                                                                                   <  1:1135153/230‑1 (MQ=255)                                                                                        tACACCAAAAAGGACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAATTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTgg                                                                                                                                   <  2:338548/239‑1 (MQ=255)                                                                                         acacCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGc                                                                                                                                  <  1:421773/239‑1 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGGGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCa                                                                                                                           <  1:1276321/239‑1 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGGGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTTACCGGTTTTCGTATCCGTTA‑AATACATTTTAACCGT‑TTTTGC‑GGGcgc                                                                                                                           >  1:1317053/1‑237 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCGCGTAGAACGGGTGCTCAGCGAGCCGACTCATCCCTCGGGGGGATTAACCGATTTTTGTATCCGTTA‑AATACATTTTACCCGT‑TTTTGC‑AGGccc                                                                                                                           >  1:512785/1‑238 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGCGCTGCGCGGGGAGAAAAGGGGCTCAGCGAGGCCCCGCCTCCCCCGGGGGACGTTACAGATTTTTGTAGCCCGTA‑AATAAAAATTACCGGT‑TTTGGG‑Agggcg                                                                                                                           >  2:624984/1‑236 (MQ=255)                                                                                                    tACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGGTCTTCGCGTGTAGAACGGGGGCTCCGCGAGCCGCCACATCCCTCGGGGGGATTAACCGGTTTTTGTATCCCTTA‑AATAAATTTTTACTGT‑TTTTGC‑AGGccacca                                                                                                                        >  1:368106/1‑237 (MQ=255)                                                                                                    tACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGGGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGCGCTCGGCGGGCAGGACGGGTGCTCAGCGGCGCCCACCCTACCTCCGGGGATTTAACCGGTGTTTTGATCCCTTACAATA‑ATTTTACCGGTGTTTggtgggctacaca                                                                                                                        >  2:963989/1‑228 (MQ=255)                                                                                                              aaaGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGGCCGCCGTTTTCGCCGGTAGAACCGGTGGTCAGCGGGCCGCATACTCCCCCCGGGGGATTAAACGGTTTTTTTATCCGTTA‑AATACATTTTAACCGT‑TTTTGG‑AAGCCAGCAAatttttggggt                                                                                                            >  2:591246/1‑232 (MQ=255)                                                                                                                                ggTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCGTGTAGAACGGGGGCTCAGCGAGCCGCATCATCCCTCGGGGGGATTAACCGATTTTTGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGCAAGGCCAGCAAATTTGGGGGCTTGCTTAGCCGGGCCtt                                                                                            >  1:536407/1‑238 (MQ=255)                                                                                                                                         aaaaTTTTGACGGTGGCGGGTGGGAAGTGGTTGCTGGGGGGGAAGAAGGGCGGGGGAAAAAATTAAGCCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGGGTGTAAAACGGTTGCTCAGCGAGCCGCATCATCCCTCGGGTGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  2:906926/239‑1 (MQ=255)                                                                                                                                         aaaaTCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  1:859797/239‑1 (MQ=255)                                                                                                                                         aaaaTCTGGACGGGGGCGGCGTGGAAGTGGTTGCTTGCGGTGAAGAGGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  1:340488/239‑1 (MQ=255)                                                                                                                                          aattttgagcGATGGAAGGGGGGAGGTGTTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATGAAGGCAGTGGTAAAAGAGGG‑GC‑GGCCCGGGTTCTGCGCGTGTGGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  2:368106/230‑1 (MQ=255)                                                                                                                                          aaattgtGACGGTGGCAGGGTGGAAGTGGTTGCTTGGGGGGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCGGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:591246/233‑1 (MQ=255)                                                                                                                                          aaaTTTTGGCGGTGGACGGGTGGAGGTGGTTGTTTGGGGTGAAGAAGGGCAGGTGGAAAAATTAAGGAATGGGTTAAAGAGTG‑GC‑GGCCCGCTTTCTGGGGGTTTAGACCGGGGGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  2:335284/239‑1 (MQ=255)                                                                                                                                          aaaTTTTGACGGTGGGAGCGTTGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAAGGCAGGTGTTAAAGAGGG‑GC‑GGCCCGTGTTCTGGGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:624984/239‑1 (MQ=255)                                                                                                                                          aaaTCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTAAAGAAGGGCAGGTGGAAAAATTAAGGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:907540/239‑1 (MQ=255)                                                                                                                                                      tggaggggTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCATTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAg                                                                     <  1:1195444/231‑1 (MQ=255)                                                                                                                                                       tgaggggTGGAGGGGTTTGGTTGGGGTGAAGAAGGGCAGGTGGAAAATTTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTAAAACGGGTGCTAAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGc                                                                    <  2:61223/232‑1 (MQ=255)                                                                                                                                                                                 tGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCGCAGCGGGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTa                                                                                                                                              <  1:1230595/140‑1 (MQ=255)                                                                                                                                                                                   aagaagGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  2:314652/121‑1 (MQ=255)                                                                                                                                                                                   aagaagGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGAGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                >  1:314652/1‑121 (MQ=255)                                                                                                                                                                                           cgggTGGAAAATTAAGTGAAGGGGTT‑AAGGGTGTGC‑GGCCCGGTTTCTGCGGGTTTAGAACCGGGCGTCGTCGTGTCGGATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                <  2:892202/236‑1 (MQ=255)                                                                                                                                                                                                      aTTAAGGCATGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCttt                         <  1:679387/234‑1 (MQ=255)                                                                                                                                                                                                           tGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCCCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                <  1:1279688/222‑1 (MQ=255)                                                                                                                                                                                                                tGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGGGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCACTTTTGGTGGCTTTCTTTGCCGGCCCTTTCTGTAAACGACGGTGTAAAAAACGGCGGTTACCCTTTTTTTTTCCCAAATAATATTCCATCGCCTTTACCAGcccaactc           >  2:679387/1‑231 (MQ=255)                                                                                                                                                                                                                  gTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:683677/167‑1 (MQ=255)                                                                                                                                                                                                                     aaaGAGGG‑GG‑GGCCCGCGTTCTGCGCGGGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCg     <  1:749315/239‑1 (MQ=255)                                                                                                                                                                                                                          ggg‑gc‑ggcCCGCGTTTGGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:76248/235‑1 (MQ=255)                                                                                                                                                                                                                          gTG‑GC‑GGCCCGTGTTTCGCGCGTGTAGAACGAGTGCCCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTTGCTTGCTTAGCCGGCCCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:150513/237‑1 (MQ=255)                                                                                                                                                                                                                          gTG‑GC‑GGCCCGCGTTCTGGGCGTGTAGAACGGGTGCTAAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:737342/237‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 CGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAG  >  NC_000913/1030037‑1030490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑