Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,241,337 A→C 19.5% G245G (GGT→GGG cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,241,3370AC19.5% 157.3 / 5.6 66G245G (GGT→GGGcvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (39/12);  new base C (2/12);  total (41/25)
Fisher's exact test for biased strand distribution p-value = 3.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGCTCTCATGATGCTGGATCATCGCAATTAGGGTAATCGTCAG  >  NC_000913/1241104‑1241570
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
aGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGAGGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCCGAATACCGc                                                                                                                                                                                                                                        >  1:295628/1‑238 (MQ=255)
   aaTCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTa                                                                                                                                                                                                                                      <  1:1002879/237‑1 (MQ=255)
      cgggCAAAGGCCGAAAGGGGAGGGGGGAAAAATGCCAGCCATGCGGACAATCAGAGGCGCGGAATGGCTATGGGCAGCAGATCGGTTGGGTTAACCAACAGCCGCAGGACCAAGAAAGTGGCGATTTGCGCCAGCCAGGTGAGGCCGTCGAAATGTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCACAAAGATACACCGGCAGAATGCCGCTGCGTTc                                                                                                                                                                                                                                 <  2:467417/236‑1 (MQ=255)
      ccAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTc                                                                                                                                                                                                                                 <  1:1281299/239‑1 (MQ=255)
      ccAGAAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTc                                                                                                                                                                                                                                 <  1:1129670/239‑1 (MQ=255)
        aGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGCGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAACAGGGCGATTACCCAGCAGACACCCGCCCAGATACACCGCCAGAATACCGCTACCTTcc                                                                                                                                                                                                                                >  1:1138384/1‑238 (MQ=255)
           aaaGACCGAAAGGGGACGGGCAAAGAATATCAACCATGCGGAAAAAATGAGCCCCGGAAGTGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACAGGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAgcg                                                                                                                                                                                                                            <  2:272297/239‑1 (MQ=255)
              tACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCag                                                                                                                                                                                                                         <  2:761772/239‑1 (MQ=255)
                   aaaGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGACAAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCCCAGGAGtt                                                                                                                                                                                                                    >  2:9126/1‑239 (MQ=255)
                    aaGGGGACGGGCAAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTa                                                                                                                                                                                                                  <  1:490646/239‑1 (MQ=255)
                         gACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAACGCAGCAGTTAATgag                                                                                                                                                                                                             >  1:219224/1‑239 (MQ=255)
                          cagggCGAAGAATATCAGCCAGTCGGAAAAAAGGGCGGCCGGAATGGCAATAGGAAGCAGATCGGTTGGGGTAACCGACAGCCCCAGCAACAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATgag                                                                                                                                                                                                             <  2:365528/236‑1 (MQ=255)
                          aggggCGAAGAATATCACCCATGCGGACAAAATGAGCGCCGGAATGGCAAAAGGCAGCGGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATgag                                                                                                                                                                                                             <  2:689714/236‑1 (MQ=255)
                          aCGGGGGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATgag                                                                                                                                                                                                             <  2:1057687/238‑1 (MQ=255)
                          aCGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCCCCGTAGTTAATgaga                                                                                                                                                                                                            >  1:912191/1‑239 (MQ=255)
                                       atcatcCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCCGAATACCGCTACCTTCCAGCGCAGTCGTTAATGAGAAAATCAGAAtct                                                                                                                                                                                                >  1:689714/1‑236 (MQ=255)
                                        tcatcCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCCCAGCTACACCGCCAGAATACCGCTACCTTCCACCGCCGAGGTTAATCGGAAAATCAGAATAcc                                                                                                                                                                                               >  1:413956/1‑238 (MQ=255)
                                        tcatcCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAAAACCGc                                                                                                                                                                                             >  1:422549/1‑239 (MQ=255)
                                              aTGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAAt                                                                                                                                                                                                                >  1:575735/1‑215 (MQ=255)
                                              aTGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCCCTGCCTTCCAGCGCGGTAGTTAATAACAAAATCGGAATACGGCCCCtt                                                                                                                                                                                        >  1:25578/1‑239 (MQ=255)
                                               tGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCCGAATACCGCTACCTTCCAGCGCAGGAGTTAATGAAAAAATAAAAAAACCGCCCCTTa                                                                                                                                                                                       >  2:468449/1‑239 (MQ=255)
                                                        aaTGGGCCCCGAATTGGCAATGGGCAGCGGATCGCTTGGGTAAACCAACGGCCCAAGCACCAGGAACAGGGCGATTTGCGCCAGCCAGGCGGGGCCGTCGAAATTGTGCAGGAGGCCGTGGCGGTGGCGAATAGGGCGATTCCCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTAAATGAAAAAATCAGAATACCGCCACTTAATGCCAGCa                                                                                                                                                                              <  2:295628/239‑1 (MQ=255)
                                                          tggccgcCGGATCGGCAAGAGGAGTCAGACCCTTGGGGTAAACCACAACCCCCAGCACCGGGAAAAGGGCGTTTTGCACAAGACGGGCGAGCCCTCCGAAATTTTGCAGGATCCCGTAGCGGTTGCAAATGGGGCGATTACCAAGCAGAAAACCGCAAAGATCCCCCGCCAGAATCCCGCTACCTCCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAAt                                                                                                                                                                            <  2:852446/235‑1 (MQ=255)
                                                              cgcCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGCATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCAAGCAATGGat                                                                                                                                                                        >  1:205940/1‑239 (MQ=255)
                                                              cgcCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGAACAGATACCCCGCCAGAATACCGCTACCTTCCAGCGCCGAAGTTAATGAGAAAAACAGAAAACCGCAACTTAATACAAGCAATGGaa                                                                                                                                                                        >  2:374634/1‑238 (MQ=255)
                                                              cgcCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAACAGAAACCCGCACAGATACACCGCCAGAATACCGCTACCTTCCAACGCAGTAGTTAATGAAAAAATCAGAAAACCGCCCCTTAATGCCaaaaagtgaa                                                                                                                                                                        >  2:1050299/1‑230 (MQ=255)
                                                                ctgaaaTGGCAATAGGCAGCAGATCGCTTGGGTTACCCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGAAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAACACCGCCACTTAATGCCAGCAATGGata                                                                                                                                                                       <  1:1050299/234‑1 (MQ=255)
                                                                           aTAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCAACTTAATGCCAGCAATGGATATAATcctgtt                                                                                                                                                             >  1:543381/1‑233 (MQ=255)
                                                                              ggCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCACCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGGATTAATCCGGCGGGCAg                                                                                                                                                        >  1:1081576/1‑239 (MQ=255)
                                                                                  gcagATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATAAAATCCGGCGGGCAGGGCg                                                                                                                                                    >  2:291434/1‑239 (MQ=255)
                                                                                  gcagATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGGCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGAGATAATCCGCCGGGCAggggg                                                                                                                                                    >  1:1155057/1‑237 (MQ=255)
                                                                                     gctggtgtGGTTTACCCAACCCCCCCAGCCCCAGACAGAGGGCGATTTGCGCCAGCCGGGGGGGGCCGGCAAAATTTTGCAGGATGCCGGGGCGGTTGCGAATCGGGCAATACCCCAGCAGAAAACCGCATAGATACCCCGCCAGAATCCCGCTACCTACCAGCGCAGTAGTTAATGAGAAAACCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATg                                                                                                                                                 <  2:631774/232‑1 (MQ=255)
                                                                                        cGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGAAACACCGCCAGAAAACCGCTACCTTCCAACGCAGCAGTTAATGAGGAAATAAGAAAACCGCACCTTAATGCCAGCAAAGGCaataat                                                                                                                                                                   >  2:1022290/1‑218 (MQ=255)
                                                                                                   cccAACCCCCCCAGCCACAGGAACGTGGCTTTTGGCCCCAGCGGGGGGGGGCGGTAGAAATTTTGCAGGTTGCTGTAGTGGTTGCGAAGGGGGCGATTCCCCAGCAAAAACCCGAACAAATACACCGCCAGAATCCCGCTACCTCCCAGCGCAGTAGTAAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTg                                                                                                                                   <  2:1155057/238‑1 (MQ=255)
                                                                                                   caccccagccccagcccCGGGAACAGGGTGTTTTGGCCAGGGGGGGCGGGGGCGTCGAAATTGGGAGGGAGGCCGGAGGGGTTGCGAATGGGCGAGTTCCCCAACAGAAACCCCCAAAGAAACACCGCCAGAATCCCGCTCCCTTCCAGCGCAGGAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTg                                                                                                                                   <  2:1276599/234‑1 (MQ=255)
                                                                                                      aaacgCCCCAGCACCAGGAACGTGGCGATTTGCCCCAGCCAGGGGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCag                                                                                                                                <  1:374634/235‑1 (MQ=255)
                                                                                                                     aGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGAACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCCGAATACCGCCACTTAATGCCAGCAATGGATCTAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGCAGTAAATACccgc                                                                                                                  >  1:146764/1‑238 (MQ=255)
                                                                                                                      ggAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTCCTGCCAGCAATGGATATAAACCGGCGGGCAGGGCGAGTCGGTTGATCATTTGCAGTTGTAAATGAcccgc                                                                                                                 >  1:539148/1‑235 (MQ=255)
                                                                                                                           tgggCGATTTGCCCCAGCCGGGGGTGCCTCTGAAATTTTTGCGGGCGTCCGTCGGGGTTGCGAGGGCGTCGATCCCCCAGCAAAAATCAGCACAGTGACCCGTCAAGACTACCGCTCCTTTCCGGCCAGGTGTTAAATAAGAAAAACAGAATCCCGCCCCTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAg                                                                                                           <  2:159241/237‑1 (MQ=255)
                                                                                                                           aTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCaa                                                                                                            >  1:859735/1‑238 (MQ=255)
                                                                                                                            tGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAg                                                                                                           >  1:889056/1‑238 (MQ=255)
                                                                                                                                                          tCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCCATGCGGTTGATCATTTGCAGTAGTAAATAACCCCCGCCAAGACCTATTACAATGACCAGACCAAAtttgtg                                                                             >  2:1052428/1‑235 (MQ=255)
                                                                                                                                                           aaattttttGGAGGTGCCGTGGGGGTTGGCAAAGGGGCGGTTCCCCCAGAAAAAACCCGAACAAAACCCCCGCCAAAACCCCCCCACCTCCAAGCGCAGTAGTTAATGAAAAAAACAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGc                                                                            <  2:1322422/234‑1 (MQ=255)
                                                                                                                                                                                    gTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGc                                                                                                                                                                                   >  1:131717/1‑111 (MQ=255)
                                                                                                                                                                                    gTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGCCAATGAAAATCAAGc                                                   >  1:801918/1‑239 (MQ=255)
                                                                                                                                                                                    gTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGCCAATAAACAcccccc                                                   >  2:938304/1‑236 (MQ=255)
                                                                                                                                                                                    gTTGCAAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGc                                                                                                                                                                                   <  2:131717/111‑1 (MQ=255)
                                                                                                                                                                                       gCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCag                                                                                                                                                                                                                         <  1:834482/71‑1 (MQ=255)
                                                                                                                                                                                       gCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCag                                                                                                                                                                                                                         >  2:834482/1‑71 (MQ=255)
                                                                                                                                                                                       gCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGAt                                                                                                                                         >  1:968864/1‑150 (MQ=255)
                                                                                                                                                                                               ggCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTa                                                                                                   >  1:1254618/1‑180 (MQ=255)
                                                                                                                                                                                               ggCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGCAAATCCAACTAATAtttcc                                         >  2:1098891/1‑235 (MQ=255)
                                                                                                                                                                                               ggCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCAAGCTAATATTGct                                         >  1:139983/1‑238 (MQ=255)
                                                                                                                                                                                                gctaTTCCCCAACAGAAAACCGCAAAAATCCCCCGCAAGAATCCCGCTACCTTCAAGCGCAGTAGTTAATAAAAAAATCAAAATCCCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGct                                         <  1:291434/234‑1 (MQ=255)
                                                                                                                                                                                                    tttaccccAGAAAAAACCCCCACAAACACCCCGACAAAATCCCGTGCCCTCCCAGCGAATTAGTAAATAAAAAAACCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGCTCTCa                                     <  1:1254934/234‑1 (MQ=255)
                                                                                                                                                                                                           cagcagAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTaaa                                                                                                                         >  1:822823/1‑148 (MQ=255)
                                                                                                                                                                                                                 aaaaCCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGCTCTCATGATGCTGGatca                        >  1:414870/1‑239 (MQ=255)
                                                                                                                                                                                                                        cacaGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCACGACCACTTACAATGCCCCGTCCAAATTGATGCAGAATATCGACAAAGAACATCCAGCTAATATTGCTCTCATGATGCTGGGAAATCGCaaa                 >  2:894562/1‑238 (MQ=255)
                                                                                                                                                                                                                        cacaGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAAAATTGCCCTCATAATGCTGGATCAACGCAAt                 >  2:215469/1‑239 (MQ=255)
                                                                                                                                                                                                                                  ccgccgAAATCCCGTTACCTGCAAGCGCAGAATGTAAGGAAAAAATCAAAATGCCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGCTCTCATGATGCTGGATCATCGCAATTAGGGTAATc       <  1:370265/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCACATTATTGCAGAATATCGACACTGAAAATCCAGCTAATATTGCTCTCATGATGCTGGATAATCGCAATTAGGGTAATc       >  1:66631/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGCCAATAAACATCCAGCAAAAATTTCTCTCTGGATGCGGGGTCAACGCATTTAGGGGaacagc     >  2:495324/1‑232 (MQ=255)
                                                                                                                                                                                                                                       aGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGAc                                                                 >  2:477289/1‑176 (MQ=255)
                                                                                                                                                                                                                                       aGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGAAGAAAATCGCAAATGCACATCAAGCTAAAATTCCTTCCATGATGCTGGATCATCGCAATTAGGGTAATCTTCaa  >  2:444345/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGGAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTTGTGCAGAAAATCGACAATGAACATCCAGCTAATATTGCTTTCATGTTACTGGAAAAACAAAATTAGGGAAATCGGCaa  >  2:710071/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aGAATACCGCTACCTTCCAGCGCAGTAGTAAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGAc                                                                 <  1:477289/176‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
AGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCCAAGACCTATTACAATGCCCAGACCAAATTGTTGCAGAATATCGACAATGAACATCCAGCTAATATTGCTCTCATGATGCTGGATCATCGCAATTAGGGTAATCGTCAG  >  NC_000913/1241104‑1241570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: