Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,251,973	T→C	15.9%	L303P (CTC→CCC)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,251,973	0	T	C	15.9%	188.9 / 4.4	75	L303P (CTC→CCC)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base T (48/14);  new base C (3/10);  total (51/24)
Fisher's exact test for biased strand distribution p-value = 3.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCG  >  NC_000913/1251741‑1252206                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          cAATTTATAAAGTCCCAGGGGGCGGGGGTTTGGCCCCTTTGCCGC‑AGGGCAAGTAGGGGGAGGGAAAACCACAGAACTTTAAATTTTCCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATc                                                                                                                                                                                                                                      <  1:1261042/239‑1 (MQ=255)  aaTTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCAt                                                                                                                                                                                                                                       >  1:1020593/1‑236 (MQ=255)           aTGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTTTTTCCCCATCCTGGGGaaaaa                                                                                                                                                                                                                           >  2:566428/1‑236 (MQ=255)                cAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGaccgaatgc                                                                                                                                                                                                                       >  1:1061158/1‑230 (MQ=255)                  ggcggcGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGc                                                                                                                                                                                                                    >  1:1325656/1‑239 (MQ=255)                                       ttGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAAttt                                                                                                                                                                                                >  1:1069069/1‑238 (MQ=255)                                        tGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAAATGCGGCCGTTGATGACCAGTCAATTAg                                                                                                                                                                                               >  2:58740/1‑239 (MQ=255)                                                                    aacccgtGAACTTTTAACTTTCCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCata                                                                                                                                                                    <  1:566428/233‑1 (MQ=255)                                                                            aaCTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGGAAAGTCAGCCATACCTTCGCTCATATGCCACAg                                                                                                                                                            >  1:766706/1‑239 (MQ=255)                                                                            aaCTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATAc                                                                                                                                                                               >  1:500856/1‑220 (MQ=255)                                                                                 ttAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACg                                                                                                                                                        >  1:382257/1‑238 (MQ=255)                                                                                 ttAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAATAAACCAAACCTTCGCCCAAAAGCCAAAGGagg                                                                                                                                                        >  2:536964/1‑236 (MQ=255)                                                                                   aaCGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCTTATGCACCAGGACGAt                                                                                                                                                      >  1:175526/1‑238 (MQ=255)                                                                                        tACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGCAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:414858/1‑239 (MQ=255)                                                                                         aCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATGATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGAGCCGGCAGTTGTTGACCAGTCAATTAGGAAAAGTAAGCCATACCTTCCCCCATATGCACCAGGAAAAACCGCaa                                                                                                                                                >  2:37324/1‑238 (MQ=255)                                                                                         aCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:32458/1‑238 (MQ=255)                                                                                         aCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:770110/1‑238 (MQ=255)                                                                                         aCCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTAATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGA‑CGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGGGGAACAGATGCGGCAGTGGCAGACCAGTCACTTCGGCAAAGACAGCCAAACCTTCGCTCATTTGCCAAAGGCCGAACCGCaa                                                                                                                                                >  2:87787/1‑238 (MQ=255)                                                                                               cgTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGGAAACccgc                                                                                                                                          >  1:264375/1‑238 (MQ=255)                                                                                                     ggaacaaGCAAAAAAACCGGCAAATCCGGTCAAACACGTAAAACCACCCTGTAAAAGCCAG‑CACCAGTTTATCGATCGGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCCCAATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGa                                                                                                                                   <  1:762216/237‑1 (MQ=255)                                                                                                     gCAAAAAGAAATAAAACAGGCACACCCGCTAAAACACGAAAAACCAACCTGTAAAAGCAAG‑CACCAGTTAATGGAGGGGTTG‑ATAACCTTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAATTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGa                                                                                                                                   <  2:1020593/239‑1 (MQ=255)                                                                                                         caaGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCCCTTGATTCa                                                                                                                               >  1:1162494/1‑239 (MQ=255)                                                                                                           aGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑TGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCAtt                                                                                                                             >  1:615370/1‑239 (MQ=255)                                                                                                           aGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATCCGGCAGTTGATGACCAGTCAATTAGGAAAAGACAGCCAACCCTTGGTGCATATGCCCAAGGCCGATCCGACAACCCGCCGATTGTTTCttt                                                                                                                             >  1:1318887/1‑239 (MQ=255)                                                                                                            gCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGGAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCtttt                                                                                                                            >  1:807561/1‑239 (MQ=255)                                                                                                                tAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTGGCTCAAATGCCCCATGACAGACCCCAAACCCCCCCCTGGAATCATTTTGGt                                                                                                                        >  2:1051268/1‑239 (MQ=255)                                                                                                                       ggCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAg                                                                                                                  >  1:680893/1‑238 (MQ=255)                                                                                                                       ggCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATCCGGCCGTTGATGACCAGTCCATTAGGACAAGTCAGCCCTACCTTCGCCCATATGCCACAGAGCGATGCCCAAACCCCCCCCTTGATTCATTTTgaccgacggg                                                                                                                 >  2:715199/1‑230 (MQ=255)                                                                                                                        gCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggc                                                                                                                >  1:1127884/1‑239 (MQ=255)                                                                                                                            aTCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCAAATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTctgcctcttata                                                                                                            >  1:78294/1‑228 (MQ=255)                                                                                                                               cgttCAAACCCGTAGAAGCAACTTTTGAAAGCCAA‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGAAAATAGAAACGC‑AGGGAACCAGTTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGAGGCGGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                                                         <  1:1296017/236‑1 (MQ=255)                                                                                                                                    aaaCCCGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTT‑ATACCCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGTTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                                                                    <  1:916654/239‑1 (MQ=255)                                                                                                                                    aaaCACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATACCCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                                                                    <  1:536964/239‑1 (MQ=255)                                                                                                                                    aaaCACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAAACCGCCGCTTGATTCATTTTGGCCGCCAGGCGgcgcgcggaa                                                                                                     >  2:551658/1‑234 (MQ=255)                                                                                                                                      acacGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGCTGCGGCAGCTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTGCCCCATATGCCACAGGCCGATCCGCAACCCCCCCCCTTGATTCTTTTTTGTCCCCAGGCTGCCCGCAgagcct                                                                                                  >  2:82456/1‑234 (MQ=255)                                                                                                                                          gTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCa                                                                                                                                                                                                        >  2:1306127/1‑133 (MQ=255)                                                                                                                                          gTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCa                                                                                                                                                                                                        <  1:1306127/133‑1 (MQ=255)                                                                                                                                                  caCCTTTTAAAACCAGCCCCCAGTTTATTGATGCGGTG‑AAACCCCATAACCAGATAAAAGAAACGC‑AGGGAACCCGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATAAGGAAAAGGCAGCAATTCCTCCGCTCATGGGCCACAGGCCGATCCGAAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                                                       <  2:32458/238‑1 (MQ=255)                                                                                                                                                   cccTTTAAAAGCCAG‑CACCAGTTTATGGATGCGGTG‑ATAACCCTTAACCCGATAATAGAAACGC‑AGGGAACCAGTTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     <  1:58740/238‑1 (MQ=255)                                                                                                                                                   aCCTTTAAAACCCAG‑CACCAGTTTATTGATGCGTGT‑AAAACCCTTAAACCAATAATAGAACCGC‑GGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATCCCTTCGCTCATATGCCGCAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     <  1:744113/239‑1 (MQ=255)                                                                                                                                                        tGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGTAAAGTAAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCAGCCGCTTGATTCATTTTGGTCCCACGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTtttg                                                                                >  1:1331581/1‑239 (MQ=255)                                                                                                                                                         gAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGTTCCATCCTGTGGAACAGATGCGGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTgg                                                                               <  1:1302335/239‑1 (MQ=255)                                                                                                                                                         gAAAACCGG‑CCCCAGTTTTTTGATTGGGTT‑ATCACCCTAAAACCTATAATAAAAACGC‑AGGGAACCGGTTTTA‑TTTTGTTGCTCACTCCTGTGAAACAGAGGCGGCGGTTGATGACCAGTCAATTAGGAAAAGTCAGCCAATCCTTCGCTCATATGCCCAAGGACGATCCGAAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTgg                                                                               <  2:1331581/239‑1 (MQ=255)                                                                                                                                                               cAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCACCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGggaaga                                                                         >  1:141040/1‑239 (MQ=255)                                                                                                                                                                     cccattttttGGTGCGTTGTATACCACTAAAACCGATAAGAGAAACGA‑AGAGAACCAGCTTTA‑TTTTTTTGCTCCACCCTGTGAAACAGGCGCGGCAGTGGATGGCCAGTCATTTAGGAAAAGCCAGCCATACCTGCGCTCATATGCCACAGGACGTCCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGc                                                                     <  2:1061158/235‑1 (MQ=255)                                                                                                                                                                        gcttATTGAGGCACTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTCATGCTCCATCCGGTGGAACAGAGCCGGCAGTTGATGATCATTCAGTTAGGAAAAGGCAGCCCTACCTTGGACCATATGCCCCAGGACGCTCCGCAAACCCGCCACTTGACTCCTTTTGTACGCCAGTCGCCCAGCGTAACCATTCCTGTCCACCTTTGTGGGAAAGAgggggggg                                                                 >  2:1333924/3‑234 (MQ=255)                                                                                                                                                                              tgagtgggTG‑ATAACCCTTAACCCGATAATAGAAACGC‑AGGAAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACATATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                                                           <  1:1012700/233‑1 (MQ=255)                                                                                                                                                                                aTGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGc                                                          >  1:669065/1‑238 (MQ=255)                                                                                                                                                                                  gCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGGGAAGAGGGCGTGGGAAAGGcccg                                                       >  1:676858/1‑236 (MQ=255)                                                                                                                                                                                   cGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACt                                                       >  1:314783/1‑238 (MQ=255)                                                                                                                                                                                   cGGCG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACCGGACGATCCGCAAACCCGCCGCCTGATTCATTTTGGTCCCCAGGCGGGCCGCCGTAGCGTTCCGTACCTACTTTGTGGAGAAAAGGGGGTGGGCCAGGAAcg                                                       >  2:467904/1‑237 (MQ=255)                                                                                                                                                                                    ggTG‑AAAACCCTTAAACCGATAATAGAACCGC‑AGGGAACCAGCTTTA‑TTTTGTGGCCCCATCCTGTGGACCAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTg                                                      <  1:754224/238‑1 (MQ=255)                                                                                                                                                                                     gTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                                                            >  2:634043/1‑183 (MQ=255)                                                                                                                                                                                      gttaTAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                                                            <  1:634043/180‑1 (MQ=255)                                                                                                                                                                                        ttattcccTTTAACCCAAAAAAGAAACCCGAGGAACCCATTTTTT‑TTTTGTTGCCCCATCCTGTGGAACAGAGTCGGCAGTTGATGCCCAGACAATTAGGAAAAGTCAGCCATCCCTTCGCTCATAGCCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCt                                                    <  2:770110/233‑1 (MQ=255)                                                                                                                                                                                              ctttAACCCGTTAATAGAAACGG‑GGGGAACCATTTTTT‑TTTTTTTGCCCCATCCTGGTGACAGGGTGGGGGAGTTGATCACCAGTCAATTAGGAAAAGTCAGCCATCCCTTCGCTCATATGCCACGGGACGATCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg                                            <  2:676858/237‑1 (MQ=255)                                                                                                                                                                                              ccTTAACCCGATAATAGAAACGC‑AGGGAACCGGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg                                            <  1:37324/239‑1 (MQ=255)                                                                                                                                                                                                 tAAACCGATAATAGAAACGC‑AGGGAACCAGTTTTATTTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaag                                                                          <  1:951332/207‑1 (MQ=255)                                                                                                                                                                                                 tAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTATTTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaag                                                                          >  2:951332/1‑207 (MQ=255)                                                                                                                                                                                                     aagagaataGAACCGG‑AGGTATCCAGTTTTA‑TTTTGTTGCCCTAGCGTGTGGAACAGATGCGGCATTTGAGCACCAGTAAATTGGGAAAGGTCACCCATCCCTTCGCTCATATGCCACGGGACGTTCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCa                                     <  2:255543/234‑1 (MQ=255)                                                                                                                                                                                                        ataataGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGTTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTTGGGGAAAAAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCaaaa                                  >  2:977342/1‑239 (MQ=255)                                                                                                                                                                                                        ataataGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTTTGGAGAAGAGGGCGTGGGCAAGGCCCTGCTAAATCAGGCCATTCATaa                                  >  2:1092112/1‑239 (MQ=255)                                                                                                                                                                                                        ataataGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCCCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGGTGGCTGGGGCAAGGCACTGCTAAGTCAGGCAATTCCTaa                                  >  1:1210525/1‑239 (MQ=255)                                                                                                                                                                                                        ataataGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCCGCAGGCGCCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATaa                                  >  1:535483/1‑239 (MQ=255)                                                                                                                                                                                                        ataataGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCAGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACCGGACGATCCGCAAACCCGCCGCTTGATTCATTTTTGTCGCCAGGTGGCGCGCGGTACCTTTCCTGTCCTGCTTTGTGGGGAAAGGGGCGGTGGCAGGGCACTGCTAAGTACGGCAATTctaaa                                  >  2:1074851/1‑235 (MQ=255)                                                                                                                                                                                                                  cGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGCTTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGGGGGCAAGGCACTGCTAAGTCAGGCACTTAATAATGAAagcgag                        >  2:264658/1‑239 (MQ=255)                                                                                                                                                                                                                  cGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAagg                           >  1:255543/1‑235 (MQ=255)                                                                                                                                                                                                                       gggAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGGGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAaacgaggggg                    >  2:1085420/1‑235 (MQ=255)                                                                                                                                                                                                                        ggAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCCCCGCTTCATTCATTTTTGTCGCCACGCGGGGCCCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGGCCCTCTAAGGCAGGCAATTCTTAATGAAAACGCGCGggc                   >  2:1332852/1‑239 (MQ=255)                                                                                                                                                                                                                          aaCCAGTTTTA‑GTTTGTTGCTCCAACCGGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACg                                                                                                                                                        <  2:648262/104‑1 (MQ=255)                                                                                                                                                                                                                           aCCGGCTTTT‑TTTTGTTGCCCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCATGTAGGAAAAGTCAGCCATCCCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGtgctgc                <  1:1051268/239‑1 (MQ=255)                                                                                                                                                                                                                                cTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     >  1:1011815/1‑165 (MQ=255)                                                                                                                                                                                                                                    a‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAAc                                                                                                                                              >  1:648262/1‑104 (MQ=255)                                                                                                                                                                                                                                           ttgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCCAACCCGGCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGGCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGAGTGCTGCAGGTCCTTATATCg  >  1:305741/1‑238 (MQ=255)                                                                                                                                                                                                                                           ttgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCCAGGCCCTGCTAAGCCAGGCAATTCATAATGAAAACGAGCGGGCGGCAGGTCCTTATATcc  >  2:661254/1‑237 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          CAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAACGC‑AGGGAACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCG  >  NC_000913/1251741‑1252206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑