Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,879,473	A→C	21.0%	V38G (GTG→GGG)	yoaG ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,879,473	0	A	C	21.0%	160.3 / 10.8	75	V38G (GTG→GGG)	yoaG	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (47/10);  new base C (2/15);  total (50/25)
Fisher's exact test for biased strand distribution p-value = 1.72e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAATGTTGTGCCA  >  NC_000913/1879241‑1879705                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         tGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCTGGGGGGCAAGACTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATAAACGATCGGTGATAATTACCAACCACTAACATCC‑AGTTCGTTTTCCGGGGCAAAAAAAAGAACGGAATTACACACAAATTTTATCACTTcg                                                                                                                                                                                                                                     >  2:319788/1‑237 (MQ=255)  gACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAAAACGTACGGAATTCACCAAACCTTTTATCACttaac                                                                                                                                                                                                                                    >  2:1337732/1‑235 (MQ=255)              aTTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATAAACGATCAGTGATATTTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGAATTCACCAGTGCTTTTGTAACTTCACCCGCCATTTCTg                                                                                                                                                                                                                        >  1:403486/1‑238 (MQ=255)               ttGTTCCCTCCCTTATCTGTTTTGCATTCGGGGGTTTGTTGTTGAAAAAAAGCATAGCCCAACATCGGGAGGGCAAGATTTGTTGCGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCCCTATCAACGATCAGTGATAATTCCCAACCAAAAACATCA‑TGTTTGTTTTCCGTGTAATAAGAACGTACGGTATTCACCAGATCTTTTATCATTTCAGCCGCCACTTCTGGc                                                                                                                                                                                                                      <  1:1100991/239‑1 (MQ=255)                   ttCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCAcc                                                                                                                                                                                                                   >  1:268609/1‑238 (MQ=255)                    tCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAAAGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCa                                                                                                                                                                                                                  >  1:472460/1‑238 (MQ=255)                     ccTCCCTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAAATTTTTTATCACTTCAGCCGCCACTTCTGGCAcaaca                                                                                                                                                                                                                >  2:451580/1‑235 (MQ=255)                             tttttttttGTGTTAGGGGGTTAGTCGATAAAAAAACGCATACCACAACATCGGGAGGGCAAGTTTTGGGACGAGCAT‑CACGGAGGTTTTTTTTCCGATGGCACAGAATTGCCCCCAACACCAACCAGTAAAAATTCCAAACCACAACCTTCA‑TGTTCTTTTTCCGTGTCATAAGAACGTCCGGTATTCACCAGATTTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCAt                                                                                                                                                                                                        <  2:852696/234‑1 (MQ=255)                             aacTGTTTTGCATTAGGGGGTTAGTCGCTGAAAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCCCCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCAt                                                                                                                                                                                                        <  1:1339102/237‑1 (MQ=255)                                 gTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCCCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGCCATACGAACGGACGGTATTCACCAACTCTTTTATCCCTTCCGCCGCCAATTCTGGCACCCGCCAAACCACCGGc                                                                                                                                                                                                    >  2:230128/1‑239 (MQ=255)                                   tttGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGCCATAAGACCGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGCATTCGGCg                                                                                                                                                                                                   >  1:825211/1‑238 (MQ=255)                                     tGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGGAGAGCAT‑CACGGAGGTTTTTTTTGCGAGGGCGCAGAAATTGAGCCATCAACGATCAGTGATATTTACCAAACACAAAACACT‑AGTTCGTTTTCCGGGTACTAAGAACGGACGGAATTGCCCAGATCTTTTATATCTTAAGCAGCAACTTCTGGCACCAGCAAAGTcctgggagtac                                                                                                                                                                                                >  2:1278985/1‑229 (MQ=255)                                     tGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGTTATTCACCAGATCTTTTATCACTTCCGCCGCCACTTCTTGCCCCACCAAATTCATCGGCgcc                                                                                                                                                                                                 >  1:1005252/1‑236 (MQ=255)                                     ggaaTTAGCGGGATAGTCGCTGAGAAAAAGCATAGCACAACATCGGGTGGGCAAGATTTGTGTCGAGCAT‑CACGGAGGTTTTTTTTTCGATGGCGCAGAAATTGCGCCATCAACGCTCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGGTTCATAAGAACGTACCGCATTCACCAGATCTTTTATCCCATCAACCGCCACTTATGCGAACAGCAAAGACAtacgggcc                                                                                                                                                                                                 >  2:1278959/4‑231 (MQ=255)                                     ggCATTAGGGGTTTAGTGGCTGATAAAAAGCAAAGCACAACATCGGGAGGGAAAGATTTGTGCCGAGCAT‑CACGGAGTTTTTTTTTGCGATGGCGCAGAAATTGGTCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGtct                                                                                                                                                                                                <  1:1148202/238‑1 (MQ=255)                                       cATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCCGCCAAGTCATCGGCGTctct                                                                                                                                                                                              >  1:1320173/1‑239 (MQ=255)                                            gCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGGTAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAAATCTTTTATTACTTCAGCCGCCACTTCTGGCACCAGACAAGCAAACGGCGTCTCTTTTTc                                                                                                                                                                                         >  2:1224193/1‑239 (MQ=255)                                            gCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTc                                                                                                                                                                                         >  1:1268647/1‑239 (MQ=255)                                                          gATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGCCCCTGTTTCATAATCGCCAGaa                                                                                                                                                                            >  1:275204/1‑238 (MQ=255)                                                                     tAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCCCCAGCAAAGTCATCGGCGTCTCTGTTTCATAAACGACAGAAACGCCCTTGcgg                                                                                                                                                                >  2:1264359/1‑237 (MQ=255)                                                                        gacaacaTGGGGGGGGAAAGTTTTGGGAAGAGCAC‑CGGGGAGTTTTTTTTGGCGATGGCGCAAAAATTGGACCATCAACGATAAGGGATAATTCCCAACCACAAACATCA‑TGTTCTTTGTCCGTGTAAAAAGAACTGACGGTATTCAACAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTa                                                                                                                                                             <  2:357642/238‑1 (MQ=255)                                                                                  ggAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCCCCAGCAAAGTCATCGGCGTCCCTGTTTCATAATCGACAGAAACGCCATTCCTGTTATTGGGGACgg                                                                                                                                                   >  2:58709/1‑239 (MQ=255)                                                                                  ggAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGAATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGAACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCCGTTCTTGTTTACg                                                                                                                                                    >  1:1103628/1‑238 (MQ=255)                                                                                   gggggAAAGTTTTGTGACGGGCAT‑CAGGGGGGTTTTTTTTGCGATGGCGCAAAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTCCGGTGTCATAAGACCGTACGGTATTCACCAGATCTTATATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACgg                                                                                                                                                   <  1:177064/236‑1 (MQ=255)                                                                                    gggtcAAGATTGGTAGCGAGCAT‑CACGGAGGTTTGTTGTGCGATGGCGCAGAAATTGCCCCAACAAGGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCATTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGt                                                                                                                                                  <  1:1358/234‑1 (MQ=255)                                                                                    aGGGCAAGTTTTGTGCCGA‑CATACACGGAGGTTTTTTTTGCGATGGCGCAAAAATTGCCCCACAAACGATCAGTGATAATTACCAACCAAAAACATCA‑TGTTCGTTTCCCGTGTCATAAGAACGTACGGTATTCCCAAGATTTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGt                                                                                                                                                  <  1:1264359/238‑1 (MQ=255)                                                                                     gggCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTggggcaggtac                                                                                                                                                >  2:629070/1‑230 (MQ=255)                                                                                     gggCAAAATTTGGGACGACCAT‑CCCGGAGGTTTTTTTTGCGATGGCGAAAAAATTCCGCCACCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTc                                                                                                                                                 <  1:16592/238‑1 (MQ=255)                                                                                      ggCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACCGAAACGCCATTGCTGTTATTGGTGACGGTCAc                                                                                                                                               >  1:802846/1‑239 (MQ=255)                                                                                       gCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACg                                                                                                                                              >  1:188702/1‑239 (MQ=255)                                                                                                gtgACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGtt                                                                                                                                     >  1:781022/1‑239 (MQ=255)                                                                                                   aCGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCCAAGTCATCGGCGTCTCTGGTTCATAATCGCCAGAAACGCCCTTGCTGTTATTGGTGACGGTACCGGGATAAGTTGCt                                                                                                                                  >  1:1273364/1‑239 (MQ=255)                                                                                                              gggggtttttttttGCGAGGGCAAAAAAATTGCCCCAACAACGAAAATTAAAAATCCCCACCCA‑AAACCTCACTGTTTGTTTTCCGTGTCAAAAGACCTCACGTTTTTCCCAAGATCTTTTATCACTGCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGa                                                                                                                        <  2:825211/233‑1 (MQ=255)                                                                                                                      ttttttGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATa                                                                                                                                                                                      >  1:349021/1‑169 (MQ=255)                                                                                                                      ttttttGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGGATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCCAAGTCATCGGAGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGGGGAGGCAACGGGATACGTTGCTTGGCCCagggttacttt                                                                                                                 >  2:806708/1‑228 (MQ=255)                                                                                                                         tttGCGATGGCGACAAAATTGCCCCATCAACGATCATGAATAATTCCAAACCAAAAACCTCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCCCCAGATCTTTTATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCg                                                                                                             <  1:109373/239‑1 (MQ=255)                                                                                                                         ttggCGATGGCGCAAAAATTGCGCCATAAACAATCGTTAATACTAACCAACCACAAACATTA‑TGTTCTTTTCCCGTGTAAAAAGAACGTCCGGTATTCACAAGATCTTTTATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCg                                                                                                             <  1:601235/236‑1 (MQ=255)                                                                                                                             cGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCAACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACCGAAACGCCATGGCTGTTATTGGTGACGGTCACGGTATAAGTTGCTTTGCCCATTATTAATTTCCCGTTat                                                                                                         >  2:220064/1‑239 (MQ=255)                                                                                                                                             gcgcCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCgttg                                                                                         >  1:1305524/1‑239 (MQ=255)                                                                                                                                             gcgcCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCgttg                                                                                         >  1:451902/1‑239 (MQ=255)                                                                                                                                                  aTCAATGATCATTAATACTTACCAACCCAAAACTTCT‑TTTTCTTTTTCCGTCTCATAAGAACGTACGGTATTCACCAGTTCTTTTATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTgcg                                                                                    <  2:451902/239‑1 (MQ=255)                                                                                                                                                           cAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTTTTGCGCACCTTCCa                                                                           >  1:842722/1‑239 (MQ=255)                                                                                                                                                            aGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATTGGCGTCTCTGTTTCATAATCGACAGAAACACCATTGCTGTTATTGGTGCCGGTCACGGTATACGTTGCTTTGCCCCTGATTCATTTCCCGTAATGAAGGACTTTCCGTTTTTGGGCACCTTCCaa                                                                          >  2:687678/1‑238 (MQ=255)                                                                                                                                                             gtattAATTACCAACCCAAACCTTTT‑TTTTCTTTTTCCGTGTCATAAAAACTTCCGGTTTTCACCAGATTTTTTACCCCTTCAGCCGCCCCTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATc                                                                         <  2:1268647/235‑1 (MQ=255)                                                                                                                                                               taTAATTCCCAACCAAAACCTTCA‑TGTTCGTTTCCCGTGTTAAAAAACCGTCCGGTATTCCCCAGTTTTTTTACACCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAg                                                                       <  1:1337732/238‑1 (MQ=255)                                                                                                                                                                        caaccaCAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAgg                                                              >  1:780148/1‑239 (MQ=255)                                                                                                                                                                        caaccaCAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAgg                                                              >  1:362772/1‑239 (MQ=255)                                                                                                                                                                          accaCAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTTCGCACCTTCCATCAGGACTTAAGGa                                                             >  2:61212/1‑238 (MQ=255)                                                                                                                                                                            cacaAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGcc                                                          >  2:337773/1‑239 (MQ=255)                                                                                                                                                                            caaaaaCCTGA‑TGTTCGTTTCCCGTGTCATAAGACCGTCGGGTTTTCCCCAGATCTTTTAACACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGcc                                                          <  1:181023/236‑1 (MQ=255)                                                                                                                                                                                  actctcgtttCCTTTTCCGTGTAAAAAGGCCGTACGGTATGCCCCAGTTTTTTTACCCCTTCAGCCGCCACTTAGGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACgaa                                                     <  1:687678/231‑1 (MQ=255)                                                                                                                                                                                      a‑tGTTCGTTCCCCGTGTAAAAAGAGCGTACGTTTTTCACCAGATTTTTTATCCGTTCGGCCGCCACTTCCGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGt                                                <  1:230128/239‑1 (MQ=255)                                                                                                                                                                                          ttCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCCGGAGCCACGAAGTAGCCaa                                             >  1:560716/1‑239 (MQ=255)                                                                                                                                                                                                ttCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGa                                       >  1:896406/1‑239 (MQ=255)                                                                                                                                                                                                    gtgtCATACGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCCCCTTCCATCCGGACTTCCGGAGCACCGAAACAATCAACGTTGAAATa                                   >  2:120782/1‑239 (MQ=255)                                                                                                                                                                                                     tttCATAAGAACGTACGGTTTTCCCCATTTTTTTTACCCATTGAGCCGCCACTTCTGGCCCCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATaa                                  <  2:780148/237‑1 (MQ=255)                                                                                                                                                                                                            aGACCGTTCGGTTTTCACAAGTTTTTTTACACCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCgt                           <  1:1224193/239‑1 (MQ=255)                                                                                                                                                                                                                  tcgggTATTCCCCAGATTTTTTACCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTCGAAATAAGTATCgtcagtca                     <  1:502478/236‑1 (MQ=255)                                                                                                                                                                                                                  tACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATg                                           >  1:1329561/1‑217 (MQ=255)                                                                                                                                                                                                                  tACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGAATCgtcagcaa                     >  1:294487/1‑236 (MQ=255)                                                                                                                                                                                                                  tACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGACGTCTCTGCTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATGCATTTCCCGTTATGAATGACTTTCCGTTGTTCTGCACCTTCGACACGGCCTTCAGGATGCCCCAAGAATTTCATGTTGAAACaaattgcgctag                        >  2:1191412/1‑226 (MQ=255)                                                                                                                                                                                                                   aCGGTTTTCACCAGATTTTTTAGCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCAt                    <  1:451580/239‑1 (MQ=255)                                                                                                                                                                                                                    cGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGAGAGTCATg                   >  2:976124/1‑239 (MQ=255)                                                                                                                                                                                                                         ttCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCCCGAAGAAGTCCATGTTGAAATAAGATTCGTCAGTCATGGCTTc              >  1:559165/1‑239 (MQ=255)                                                                                                                                                                                                                            aCCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAGCAAGTCAATGTTGACATAAGTATGGTCAGTCATGGCTTAAAt           >  1:708962/1‑239 (MQ=255)                                                                                                                                                                                                                                 aTCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGCCAGAAACGCCATTGCTGTTTTTGGGGACGGTCACGGTATACGTTGCTTTGACCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCAACGCAACAGTAAATGGTGAAATAAGTAACGGCAGTCACAGTTTCAAttttgt      >  2:787876/1‑239 (MQ=255)                                                                                                                                                                                                                                 aTCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGCCAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTTCGCCCCTTCACTCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGTAATAAGTATCGGAAGTCATGGCTTCatattttg      >  2:1126437/1‑232 (MQ=255)                                                                                                                                                                                                                                 aTCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGTACTTCAGGAGCCACGAAGCAGTCAATGTTGCAATAAGTATCGTCAATCCTGGCTTCAAtgttgt      >  2:1037644/1‑239 (MQ=255)                                                                                                                                                                                                                                 aTCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAAtgttgt      >  2:1296743/1‑239 (MQ=255)                                                                                                                                                                                                                                 aTCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAAtgttgt      >  1:174468/1‑239 (MQ=255)                                                                                                                                                                                                                                  acTTTTATACCTTGAGCCGCCATTGCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCAGAATCGACAGATACGCCTTTGCTTTTATTGGTGACGTTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGCCTTTCCGTTGTTGCGCTCCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAAtgttg       <  1:787876/236‑1 (MQ=255)                                                                                                                                                                                                                                   tttttATCCCTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCa                                                                                                                                                <  1:1141084/98‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCa                                                                                                                                                >  2:1141084/1‑99 (MQ=255)                                                                                                                                                                                                                                   cTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGCAGAAGTCAATGTTGAAATAAGTATCGCCAGTCATGGCTTCAAtg          >  1:881221/1‑233 (MQ=255)                                                                                                                                                                                                                                     tttATCCCTTCCCCCGCCCCTTCCGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAATGTTGTGCCa  <  2:1305524/239‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         TGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCAT‑CACGGAGGTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCA‑TGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCACTTCTGGCACCAGCAAAGTCATCGGCGTCTCTGTTTCATAATCGACAGAAACGCCATTGCTGTTATTGGTGACGGTCACGGTATACGTTGCTTTGCCCATGATTCATTTCCCGTTATGAATGACTTTCCGTTGTTGCGCACCTTCCATCAGGACTTCAGGAGCCACGAAGAAGTCAATGTTGAAATAAGTATCGTCAGTCATGGCTTCAATGTTGTGCCA  >  NC_000913/1879241‑1879705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑