Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,274,349 T→G 21.8% G57G (GGT→GGG yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,3490TG21.8% 148.8 / 5.5 75G57G (GGT→GGGyejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (14/39);  new base G (15/5);  total (31/44)
Fisher's exact test for biased strand distribution p-value = 3.18e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGC  >  NC_000913/2274115‑2274578
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
tACCTGGTGGTAGGACGTCAATAAAGCGGCCAAACTGCCTTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                      <  1:1210311/238‑1 (MQ=255)
    tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGGCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCCCCGCCGGTGGAGGGGTTtgtg                                                                                                                                                                                                                                  >  2:265613/1‑238 (MQ=255)
    tggtggTATGACGTCAATAAAGCGGCCAAACTACCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGCCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTg                                                                                                                                                                                                                                  >  1:1276042/1‑238 (MQ=255)
        ggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCTGgccag                                                                                                                                                                                                                             >  1:1049429/1‑238 (MQ=255)
              ccggtcaaaacgCGGCCAAACGGCCGTCCGCCAGAAAACAGGGGCAGTAGGTGGGTGCTTACCTGGTTCGCCGTCTGTTGCTGGGGATCCCAACATTGTGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATgcg                                                                                                                                                                                                                      <  2:1071354/228‑1 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCTGGCCAGCCATggcca                                                                                                                                                                                                                    >  1:315683/1‑235 (MQ=255)
                   aaaaaggCGGCCACCCTGCGTCCCGCCAAAAAACAGGGGGATTAGATGGCCGCTTCCCTGTTTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCaaa                                                                                                                                                                                                                  <  2:73807/232‑1 (MQ=255)
                        aGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGGGATCCCAACATTATGGGCGGTTATCACCCTCAAATTTTTCATCGTGCCAATTGCGCCTGGCGGTCCGGTCGACCAGGCCACCGCCGCCATTTAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTGTGTACAGCCAAGGGCAAACcgggg                                                                                                                                                                                                            >  2:438656/1‑237 (MQ=255)
                             ccAAACTGCCGTCCGCCAGCAAACAGTGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAACTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGTGAAAGGGTTCGTGCCAGCCACGAGCAAACGGGTGTCGGc                                                                                                                                                                                                       >  1:287688/1‑239 (MQ=255)
                             ccAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGGTCCTGCCAGCCCAGCGCAAACGCGGGTccgg                                                                                                                                                                                                       >  1:1196378/1‑236 (MQ=255)
                                       gTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTTCCCGGCGCAGGCGGTGAAGGGGTTGGTGCCCGCCAGGCGCAAACGGGGGTGGGCAAAAACAGCg                                                                                                                                                                                             >  2:114314/1‑239 (MQ=255)
                                         cccccccgaaaCAGGGGGGGGTAGTTGGGCCGTTACCGTATTCCCCGTTTGTTCGTGGTGGTCCCAACATTTTGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                            <  2:1324729/231‑1 (MQ=255)
                                         ccccccagaaaCTGGGGGGGTGGGTGGGCGTTTTCCTGTTTCGCCGGTTGTTTATGGTGAACCCAACATTATGGGGGATTTACCCCCACAACTTTTTTATCGTGAAAATTGCGCGTGGCGGGCGGGTCGACCCGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                           <  2:766484/231‑1 (MQ=255)
                                         cccccAGCAAAGGGGGGGATTAGGTGGGGCTTTTCCTTATTCCCCGTCTGTTGCTGGGGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                           <  2:332369/236‑1 (MQ=255)
                                                               tggtGGGGGTTTCCCTGCTTCGCCTTTTTTTTCGGGTGCCCCCAACTTTATGGGCTATTTGCCCCATCAATTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                       <  2:599379/235‑1 (MQ=255)
                                                               aGATGGGGGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                       <  1:1353135/238‑1 (MQ=255)
                                                                gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGTTTCGGGCCAGCCAACCCCAAACCGGTGTCGGCAAAATACGGGACAGGAAATACCGggggggcggcg                                                                                                                                                                     >  2:170513/1‑234 (MQ=255)
                                                                  tGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCCTTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGACGGCGTTCGTGCCACCCCAGCGCAAACGGGGGTCGGGCATATCAGCGCCCGTAATTACCCTGGCGGACACggg                                                                                                                                                                   >  2:255463/1‑238 (MQ=255)
                                                                   gggCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGTGGTTGTGCCAGGCCTGCGCAAACGGGGGTCGGCCATATCAGCGCGAGGAATTACCGTGGGGGGCgggggt                                                                                                                                                                  >  2:1162952/1‑237 (MQ=255)
                                                                     gtccTTACCTGATTCGCCTTCTATTGCTGGTTATCCCAACATTATGGGCGATTATCACCATCAACTTGTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:733349/235‑1 (MQ=255)
                                                                     gcgcTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:186243/238‑1 (MQ=255)
                                                                     gcgcTTACCTGATTCGCCGTCTGGTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:750381/238‑1 (MQ=255)
                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGTGCGGACGCGGATTAGa                                                                                                                                                              >  1:1065802/1‑238 (MQ=255)
                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCCGCCATGCGCAAACGGGTGTGGGCAAAATCAGCGACAGTAATTACCGTGGCGGagccgggtttga                                                                                                                                                              >  1:1110620/1‑227 (MQ=255)
                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGGGCCAGCCATGGGCAAACGGGGGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACCCGGGTTAGa                                                                                                                                                              >  1:917526/1‑238 (MQ=255)
                                                                           aCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAAAATCAGCGACAGTAATTACAGTGGCGGACGCGGAGTAGATCCa                                                                                                                                                          >  1:1128057/1‑239 (MQ=255)
                                                                           aCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGCGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGAAATTACCGTGGCGGACCCGGATTAGATCCa                                                                                                                                                          >  1:681140/1‑239 (MQ=255)
                                                                                     ccGTTTTTTTCTGGTGTTCCCAACATTTGGGGCGTTTATCCCCATCAACTTTTTCACCGGGCAAATTGCCCCTGGCGGGCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCg                                                                                                                                                <  2:1276235/239‑1 (MQ=255)
                                                                                       gTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCCTGGCGGACCCGGATTAGAACCCGaaaccggttct                                                                                                                                              >  1:430590/1‑230 (MQ=255)
                                                                                        tCTATTGCTTGTGATCCCAACAGTATGGGCGATTATCACCATTAACTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGccg                                                                                                                                             <  1:886108/239‑3 (MQ=255)
                                                                                                ttgttacccccACTTTGTGGGCGATTATCACCCTCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACt                                                                                                                                     <  2:244839/232‑1 (MQ=255)
                                                                                                  gTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGTGAAGGGGTTCGTGCCAGCCCTGCGCCAACGGGGGTCGGCACTATCCGCCACAGGAATTACCGTGCGGGGCGCGGGTTTGATCCCAAAAGGATGGCTgagcgaaacc                                                                                                                                    >  2:694189/1‑231 (MQ=255)
                                                                                                   gttccccAACTTTTTGGGCGTTTATCCCCATCACTTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGGCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                  <  2:895753/235‑1 (MQ=255)
                                                                                                    ggtCCCAACATTAGTGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                  <  1:57686/236‑1 (MQ=255)
                                                                                                       cccAACATTCTGGGCTATTATCACCATCAACTTTTGCATCGGGAAAATTGCCCGTGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                              <  1:530299/239‑1 (MQ=255)
                                                                                                                 tGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCAATCATCGCTACGGGTTTGa                                                                                                                    >  1:493159/1‑239 (MQ=255)
                                                                                                                       aTTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCAATCGCCGACATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGc                                                                                                               <  1:58590/238‑1 (MQ=255)
                                                                                                                       aTTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGCCAGAAATTACCGTGGGGGACGCGGGTTAGAGCCAGAAATGATGCCTGGGGTAACTCATCCCTCCGGTTTTGTAAAGcc                                                                                                              >  2:900663/1‑239 (MQ=255)
                                                                                                                        ttATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                             <  1:119934/239‑1 (MQ=255)
                                                                                                                         ttttcccacttaCTTTTTTTTCCTGCCAAATTGCCCTGGGCGGGCCGGTGCACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                             <  2:729307/228‑1 (MQ=255)
                                                                                                                             aCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCCGAAGGGATAGCCGAGATCACTCATCGCACCGGGTTTGGTAAACCGAAcc                                                                                                        >  1:925118/1‑238 (MQ=255)
                                                                                                                                  cAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACCGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGGAGTGATCGCTGGAATCACTCCTCGCTACGGGTTTGATAAGCCGAGCCACGaa                                                                                                   >  1:18946/1‑238 (MQ=255)
                                                                                                                                   aaCTTTTTCATCGTGCAATTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACg                                                                                                 <  1:1162952/239‑1 (MQ=255)
                                                                                                                                         ttCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCCGCGACAGTAATTTCCGTGGCGGACGCGGGTTAGATCCCGAAAGGGTCGCTGAGGTCACCCAACCCCACGGGTTTGGTAAACCGATACCACAACGGTACtt                                                                                           >  2:280299/1‑239 (MQ=255)
                                                                                                                                             ccGTGGAAATTGCGCCTGGGGGTCCGGTCGAACCGGCCATCGCCGCCATTGATTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCaaa                                                                                       <  1:1202007/238‑1 (MQ=255)
                                                                                                                                                     atttGGGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                <  2:167134/237‑1 (MQ=255)
                                                                                                                                                     aaTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                <  1:694189/239‑1 (MQ=255)
                                                                                                                                                         gggcCTGGGGGCCCGGTCGACCAGGCCATGGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGa                                                                            <  1:280299/237‑1 (MQ=255)
                                                                                                                                                                gCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACAt                                                                      <  1:900663/238‑1 (MQ=255)
                                                                                                                                                                           cgccAGGCCATGGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGAtt                                                           <  1:255463/236‑1 (MQ=255)
                                                                                                                                                                                     tCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  2:280250/127‑1 (MQ=255)
                                                                                                                                                                                     tCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 >  1:280250/1‑127 (MQ=255)
                                                                                                                                                                                     cccccgccATTGTGTTTGGTAATGGTGGGGGTTACCCCGTGGCAGGCGGTGAAGTGGGTCTGGCCGGCCAGGCGCAAACGGGGGGCGGGAATATCAGCGACAGTAATTACCTGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGAACACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGc                                                <  2:1223671/236‑1 (MQ=255)
                                                                                                                                                                                      cgccgcCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGGGCCAGCCATGCGCAAACGGGGGGCGGCAATATCAGCGGCAGTAATTACCGGGGCGGACCCGGATTAGGTCCAGAAAGGATCGCTGAGATCACCCATCGCCCCGGTTTTTGTAAGACGATTCCCCAACGGTAATTCCAAATGCCCTGGGACTACAACCGCTTTTGGTTTGGGGGTAGcc                                               >  2:634829/1‑239 (MQ=255)
                                                                                                                                                                                       gccgccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGGCGCGGAGTAGATCCAGAAGGGATCGCTTAGATCACTCCTCGCTTCGGTTTTGGTAAACCGATCCCCGAACCTTACTTCCAAATGGTCTGGGCCTACAAACCCTTTGAttttttggaaaaccc                                              >  2:120763/1‑228 (MQ=255)
                                                                                                                                                                                             aTTGAGTTTGGGAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCAAGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGGCGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCg                                        <  1:938578/239‑1 (MQ=255)
                                                                                                                                                                                                   tttGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAAAGCCAGAAGTGATCCCTGGGATAACTCAACGCCACGGTTTTGGAAAGCCGCTCCCAGAACGTTACTTCAAAAGGGCCTGGGACAAAATCCCCTTTTATTTTGGGGTTAACCTtttttgaacgg                                   >  2:1257699/1‑231 (MQ=255)
                                                                                                                                                                                                   tttGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAAGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTTGTAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTCAATCCGCTTTGATTTTTGCGTTAGCCTGTTTCCCaagg                                   >  1:924358/1‑235 (MQ=255)
                                                                                                                                                                                                     ttgTAATGCCGGGGTGTTCCCCGGCGGCGGGTGTGAAGGTGGTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                <  2:493159/237‑1 (MQ=255)
                                                                                                                                                                                                     tGGTAATGCCGGAGTATTTCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                <  1:114314/239‑1 (MQ=255)
                                                                                                                                                                                                           tGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCaaaa                                                                                      <  1:298348/179‑1 (MQ=255)
                                                                                                                                                                                                                gggtttttcccGGCGGAGGCGGTGAAGGGTGTCGTGCCCGCCAGGGGGAAACGGGTGTCGGCAATTTCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACg                     <  2:715666/231‑1 (MQ=255)
                                                                                                                                                                                                                  ttATTACCCGGGGCAGGGGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  1:3891/237‑1 (MQ=255)
                                                                                                                                                                                                                  gttttccccGGCGCGGGGGGTGAAGGGGTTCGGGCCAGCCAGGGGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:924358/232‑1 (MQ=255)
                                                                                                                                                                                                                  gTATTCCCCGGCGCGGGGGGTGTAGGTTGTCGGGCCAGGCATGGGCAAACGGGTGGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCTCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGTCGc                    <  2:287688/238‑1 (MQ=255)
                                                                                                                                                                                                                  gTATTACCGGGCGGGGGCGGTGAAGGGTGTCGTGCCAACCATGGCCAAACGGGTTTCGGAAATATCAGCGAAAGTAATTCCCGTGGCGGACGCGGATTAGATCCAGGAGGGAGCGCTGAGATCACGCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:992145/238‑1 (MQ=255)
                                                                                                                                                                                                                  gTATTACCCGGGCCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  1:446735/238‑1 (MQ=255)
                                                                                                                                                                                                                  gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                <  2:1058203/178‑1 (MQ=255)
                                                                                                                                                                                                                  gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                >  1:1058203/1‑178 (MQ=255)
                                                                                                                                                                                                                     ttccccGGGCCAGGCGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGAt                <  2:1067915/236‑1 (MQ=255)
                                                                                                                                                                                                                           gggccAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAACCGGGTGTCAGCAAAAACAGCGACAGGAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGa          <  1:1301729/235‑1 (MQ=255)
                                                                                                                                                                                                                             cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCCAAATGCTCTGGGGCTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGGGCTGACGCTGATTTAAGACa        >  1:1067915/1‑239 (MQ=255)
                                                                                                                                                                                                                             cgcAGGAGGCGAAGGTGGTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAAGCGGATTAGATCCAGAAAGGATCGCTTAGATCACTCATCGCCACGGGTTTTAATAGCCGATCACCGACAGTTACTTCACAAAGCTCTGGGACTACATCCGCTTTGATTTTGGGGTTAACCTTTTTTCCAGCGCCACGCGGCGGGAGCGGATTaaaaaaa        >  2:1232401/1‑235 (MQ=255)
                                                                                                                                                                                                                                   gggggAAGGTTTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTg   <  2:1110620/234‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTTAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGc  <  1:68601/238‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGC  >  NC_000913/2274115‑2274578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: