Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,167 A→C 13.6% T512T (ACA→ACC yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,1670AC13.6% 183.1 / 3.3 80T512T (ACA→ACCyejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (41/22);  new base C (3/14);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 7.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAA  >  NC_000913/2281947‑2282392
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
cgGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGCATTTGCAACATGGGCACCACGAGAAAGGCGAATGGTTGAAAATCACCATTTACGAGGAAGAAGGCGCGGGTTGGAGTAGTGGTTTCCGGCTGACAAAACCCGCCCacgcaaccg                                                                                                                                                                                                                   >  2:1321882/1‑229 (MQ=255)
 ggAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCCACATGGGCACGACGAGAAAGGCGAATGGTTGGAAATCACCTATTACGAGGAAAACGGCCCGGATGGGAGTGAGCGTTTCCGTCTGCACACACACGCCCCACGGACCGcc                                                                                                                                                                                                                 >  2:654920/1‑238 (MQ=255)
 ggAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCCCGACGGGAAAGGCGCAAGGTTGACAATCACCTATTACGGTGAAGACGGCGCCGGTGTGAGTGAGCGTTTCCGTCTGCAAAAACCTGCCCaaccgaccac                                                                                                                                                                                                                  >  2:7517/1‑228 (MQ=255)
   aaaaCGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGAATTACGCTGTAGCGGCATGTCCTTGCAACCTGGGCACGACGAGAAAGGCGAATGGTTGGAAATCACCCATTCCGATGAAGACGGCCCGGAGGCGAGTGAGCGTTTCCGTCTGCAAACCCAAGCCCCACGTCCCGCCTTc                                                                                                                                                                                                              >  2:250483/1‑239 (MQ=255)
      acgattggcccgcccgcCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGag                                                                                                                                                                                                           <  1:1169097/230‑1 (MQ=255)
       ctatatGGGCGCCCGCCGCTGCCGCGAACGGGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          <  1:297418/237‑1 (MQ=255)
       cgttgtgcgcgcccgccGCTTCCGTGAATTGGACACCGTACGTGGTGAGCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          <  2:840571/230‑1 (MQ=255)
             tGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTTCAACATGGGGACGACGAGAAAGGCGAAAGGTTGAAAATCACCCATTACGATGAAGAAGGCGCGGATGTGAGTGACGGTTTTCGTCTGCCAACACCTGCCCAGCGCACCGCCCTCGggcacattt                                                                                                                                                                                                    >  2:1072999/1‑234 (MQ=255)
                  cccgccGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACCTGGGCACGACCAGAAAGGCGAATGGTTGAAAATAACCTATTACCATGAAGACGGCGCGGATGGGAGGGAGGGGTTTCGTCTTCCAACACCCGCCCCCCCGCCCGCCTTCCAGCGGCtttttttt                                                                                                                                                                                               >  2:794777/1‑236 (MQ=255)
                  cccgccGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATc                                                                                                                                                                                               <  1:867917/239‑1 (MQ=255)
                      ccgcTTCCGGGAATGTGAACCGGTACTGGTTGATCCGGACTATATGTTAAAAGCGGCGCTACGCCTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATccgcc                                                                                                                                                                                           <  2:1069565/239‑1 (MQ=255)
                        gcTGCCCGGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGTTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATccgcccg                                                                                                                                                                                         <  1:415510/239‑1 (MQ=255)
                          tgcccggAATGTGACCCCGTATGGGTTGAGCCGGGCGATATGTTAAAAGCGGCGCTACGAATGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGCCGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       <  2:549542/233‑1 (MQ=255)
                          tGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATTGTAAAAGCTGCGCTACGCCTGAAAGCCGCGCGGGTATTTCGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGCATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       <  1:89902/239‑1 (MQ=255)
                                      gACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          <  2:276958/208‑1 (MQ=255)
                                      gACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          >  1:276958/1‑208 (MQ=255)
                                      gACACCCTGCGGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACgcgc                                                                                                                                                                                <  1:1044258/234‑1 (MQ=255)
                                       gcacCGTACTGGTTTAACCGGAAGATATGTTAAAGGGGGCGTTACGAAGAAAGGTCGCGCTGGTATTACGCTGGAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAAGGGTTGAAAATCACCTATTACGATGAAGGCGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACAcc                                                                                                                                                                           <  2:1329745/237‑1 (MQ=255)
                                           cGTTCTGGTTGATCCGGACGATATGGTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCa                                                                                                                                                                      <  1:654920/239‑1 (MQ=255)
                                                tGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGCCGAGAAAGGCGGATGGTTGGAAATCACCTATTACCATGAAGACGGCGAGGGAGAGAGTGAGGGATTCCAGCCGCAAAACCCAGCCCTCCCTATCGCCTTCGGGGAGATTTGTTTCCCCCCGCACACGACCCAACCGGGCAtatg                                                                                                                                                                  >  2:1044258/1‑235 (MQ=255)
                                                  gTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       <  1:219930/215‑1 (MQ=255)
                                                  gTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGGTTCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACTCGCCCAACGGGCCTccc                                                                                                                                                                  >  1:1354715/1‑236 (MQ=255)
                                                       tCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       >  1:886188/1‑210 (MQ=255)
                                                       tCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       <  2:886188/210‑1 (MQ=255)
                                                       tCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGCACCGCCTTCGAGCAGACTTTTACCGGCCCGCATATGCGCCCACCGGGCATCCcgctgc                                                                                                                                                             >  1:50548/1‑236 (MQ=255)
                                                       tCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGGCTTTTCAACATGGGCCCGGCGAGAAAGGCGAATGGTTGAAAATCACCTATTAACATGACGACGGCGCGGGAGTGAGTGAGCGTTTCCCACCGCACACACCTGACCACCGTACCGCCTTCCAGCAGCTTTTTTTCCGCCCCCATACGCACCCACCCGCCATccccccgc                                                                                                                                                             >  2:300151/1‑231 (MQ=255)
                                                            aCGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCCCATACGCGCACACCGGGCATCCCGCCGCGCTggcgc                                                                                                                                                     >  1:383067/1‑236 (MQ=255)
                                                                tatGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTCCGATGAAGACGGCCCGGATGTGAGTGAGCGTTTCCGTCTGCAACAACCTGCCCAGCGTACCGCCTTAGAGCCGCTTTTTTTCCGCCCCCATAACCCCACACCGGGCACCCcgccgccgcgggccacc                                                                                                                                                  >  2:1082196/1‑227 (MQ=255)
                                                                 atGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCCAACCGGCCATCCCGCCCCGCTGGAtacccc                                                                                                                                                 >  1:1198975/1‑233 (MQ=255)
                                                                 atGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCCCTGCGCTGGTTCAccg                                                                                                                                                 >  1:844458/1‑238 (MQ=255)
                                                                    ttAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATccgcc                                                                                                                                                                                           <  1:1036589/193‑1 (MQ=255)
                                                                      aaaaGCGGCGCTCCGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATAACCTTTTACGATGAAAACGGCGCGGATGTGAGCGAGCGTTTCCGGTCGCAACAACCTGCACAGCGGACCCCCTTCGGGCAGCTTTTTTTCCGCCCGCATTCGCCCACCCCGCGCACCCCCCTCCGCTGGATTAcacccccca                                                                                                                                           >  2:984110/1‑231 (MQ=255)
                                                                              cgcTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCt                                                                                                                                    >  1:1252718/1‑238 (MQ=255)
                                                                              cgcTACGACTGAAAGACGCGCTGGCATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGGGAGCGGTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCCCCCGCATACGCGCACAACCGCCACCCCCCCGGGCTGGAACCCCCCCCCCGaaaaccc                                                                                                                                    >  2:591946/1‑232 (MQ=255)
                                                                                                      tATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGACCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCAccgccgccg                                                                                                                                           <  1:177955/207‑1 (MQ=255)
                                                                                                             tggTAGCGGAATGTCTTTGCAAAATGGGCACGACGAGAAAGGCGAATGGTTAAAAACCACCTATTACGATGAAGACGGCGCGGATGTGAGTGGGCGTTTCCGTCGGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATCCGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGAtt                                                                                                    <  2:844458/237‑1 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTATGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          <  2:552138/134‑1 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                                                                                                                                                                          >  1:552138/1‑134 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTg                                                                                                 >  1:483499/1‑239 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACCAGCACAACGGGCATCCCCCTGCGCTGGATAACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGAtttt                                                                                                  >  1:205905/1‑238 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCCCATACGCGCACACCCGGCATCCCCCTGCGCTGGGTCACCGCCGCCGAAATCCCCCCCCCGCAAGCCTTTTTTCGCACCCCGGAttttt                                                                                                 >  2:798909/1‑238 (MQ=255)
                                                                                                                tAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTAAGATGAAGACGGCGCGGAGGTGAGGGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGGACCGCCTTCGAGCAGCTTTTTATCCGCCCCCCACCCCCCAAACCGGGCCTCCCCCCGGGGTGGATCACCCCCGCCGCTAGCCCCGCCCAGAACACCCTATAGCGAAAACCGGGATTTg                                                                                                 >  2:1301018/1‑239 (MQ=255)
                                                                                                                     gCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCCCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCCCACCGGGCATCCCCCTGCGCTGGACCACCCACGCCGCTATCCCCCCCCAGCAAGCCTTATTGCGAACCCCGGGGTTTgggggg                                                                                            >  2:530586/1‑234 (MQ=255)
                                                                                                                        tttCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCTGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGcc                                                                                         <  1:378273/237‑1 (MQ=255)
                                                                                                                        tGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCACCGTACCGCCTTCGCGCAGCTTTTTATCCGCCCGCATACGCGCCCACCGGGCATCCCCCGCCGCTGGCTCACCGCCCCCCCAACCCCCCCCCAGCAAGCCTTTTTTCGCCACCCGGATTTTttcgcccc                                                                                          >  2:1267150/1‑234 (MQ=255)
                                                                                                                          tCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGCTGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCCCATACGCGCCCCCCGGGCATCCCGCTCGGCTCGGTCACCCCCGCCGATAACCCCCCCCACCACACCCTATTACCAACCCCGGATTTTGTCGACGcac                                                                                        >  2:320258/1‑236 (MQ=255)
                                                                                                                          tCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       >  1:1315825/1‑143 (MQ=255)
                                                                                                                          tCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCa                                                                                                                                                                                       <  2:1315825/143‑1 (MQ=255)
                                                                                                                          tCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCCCCCGCATACGCCCACACCGGGCATCCCCCTGGCCTGGATCACCCCCGCCGCAATCCTGCCCCAACAAACCCTATTTCCAACCCCCGGTTTTgtgcgcgccc                                                                                        >  2:859308/1‑230 (MQ=255)
                                                                                                                               gccagaTGGGCACGAGGAGAAAGGCGGATGGTTGAAAAACCCGTATTCGGAGGGAGGCGGCGGGGTTGGGAGTGAGCGTTTCCGCTTGCAAACCCCGGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACGGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGa                                                                                  <  2:959017/234‑1 (MQ=255)
                                                                                                                                caaccgggggACCACGGGAAAGGGCAAATGGTTAAAACTCCCTTATGACGAGGAAGCGGGGGCGGTGGTGTGGGCGCGTTTCCGTCTGAAACCCCCTGCCCAGCGTCCCGCCTTCGGCCAGCTTTTTACCCGCCCGCATCCGCGCCCACCGGGCATCCCGCTGCGCTGGATCCCCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGa                                                                                  <  2:1252718/233‑1 (MQ=255)
                                                                                                                                   cATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGCTTTTGTCGTCCCCCGCATGAAAg                                                                               >  1:1170374/1‑238 (MQ=255)
                                                                                                                                     tGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGCTATCCTCGCCCAGCAAGCCTTATTGCGCACCCCGGATTTTGTCGTCGCCCGCATGAACGGca                                                                            >  2:284117/1‑238 (MQ=255)
                                                                                                                                       gtgcgaacgaAAAGGGGGAATGGTTGAAAACCCCCTATGCCGATGAAGCGGGCGGGGTTGTGTGTGAGTTTTTCCTTGTCCAAACCCCGGCCCAGCGTACTGCCTTCGGGCAGCTTTTTACCCGCCCCGATACGCGCCCCCCGGGCCTCCCGCTGGGCTGGATCCCCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCa                                                                           <  2:135073/232‑1 (MQ=255)
                                                                                                                                         cACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGt                                                                         <  1:7517/238‑1 (MQ=255)
                                                                                                                                             acgaGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCACCCCGCTGCGCTGGATCACCGCCGCCGATATCCCCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGCCGCCGCCCGCATCAAAGGCCCGTACTg                                                                     >  2:1097353/1‑238 (MQ=255)
                                                                                                                                                 gaAAGGCTAGTGGTTGAAAATCCCCTGTTACGATGAAGACGGCCGGGATGTGAGTGAGCGTTCCCGTCTGCAAACCCCTGCCCAGCTTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAg                                                                 <  1:900808/238‑1 (MQ=255)
                                                                                                                                                  aaaGGCGAAGGGTTGAAAACCCCCTATTACGATGAAGCCGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACCCCGCCCCAGCGTCCCGCCTTCGAGCAGCTTTTTAACCGCCCGCATACGCGCACCCCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGt                                                               <  1:807478/239‑1 (MQ=255)
                                                                                                                                                    aGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCcgc                                                                                                                                                                >  1:1365347/1‑140 (MQ=255)
                                                                                                                                                    aGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCcgc                                                                                                                                                                <  2:1365347/140‑1 (MQ=255)
                                                                                                                                                           aTGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGGCGCCCGCCTGAAAGGCCAGCACTGGCCggggggggtaa                                                       >  2:257792/1‑230 (MQ=255)
                                                                                                                                                           aTGGTTGAAAACCCCCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTCCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTACCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGaaa                                                       <  1:284117/239‑1 (MQ=255)
                                                                                                                                                                tGAAAATCCCCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAgtg                                                  <  1:798909/239‑1 (MQ=255)
                                                                                                                                                                   aaaTCACCTTTTACGATGAGGGCGGCGCGGATGTGAGTGATCCTTTCCTTCTGCAAACACCTGCCCAGCGTACCGCCTTCGCGCAGCTTTTTATCCGCCCGCATCCGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGtt                                                <  1:1072999/238‑1 (MQ=255)
                                                                                                                                                                        aCCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTAtt                                                                                                                  >  1:1062457/1‑167 (MQ=255)
                                                                                                                                                                         ccTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGCCGTCGCCCGCATGCAAGGCCAGTACTTGCAGGAGCGTGGAAAAGTgattgattc                                          >  1:983736/1‑237 (MQ=255)
                                                                                                                                                                          cTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCACCCCGCTGCGCTGGATCCCCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACg                                        <  1:1097353/239‑1 (MQ=255)
                                                                                                                                                                                  aTGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCCGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGCTATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTCATGGCAGGTGGGTGGCACAGGGTTCAGTTACGAAGGCCGt                                >  1:313867/1‑239 (MQ=255)
                                                                                                                                                                                    gAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGGAAAAGGGTTCGGTTACCAAGGGCGttt                              >  1:171231/1‑239 (MQ=255)
                                                                                                                                                                                    gAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCCCATGAAAGGCCAGTACTGGCAGGGGCGGGAAAAAAAGGTCCATTACGaaagggggtt                              >  2:347707/1‑231 (MQ=255)
                                                                                                                                                                                            cgcgGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTTGTCTg                       >  1:106584/1‑238 (MQ=255)
                                                                                                                                                                                               ggggtggggtgacCTTTTCCGTCTGCAAACCCCCCCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACCCCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGgcg                    <  1:1267150/230‑1 (MQ=255)
                                                                                                                                                                                               ggATGTGAGTGAGCGTTTCCGTCTGCAAACCCCGGCCCAGCGTACCGCCTGCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGCCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGgcg                    <  1:300151/238‑1 (MQ=255)
                                                                                                                                                                                                         tggctttcccGTTTGCAAACCCCTGCCCAGGTTACCGCCTTCGAGCCGCTTTTTACCCGCCCGCATACGCGCACCCCGGGCATCCCGCTGCGCTGGATCCCCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTAcg         <  1:70188/231‑1 (MQ=255)
                                                                                                                                                                                                          ggcttttCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGTTTTTTACCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTAcg         <  1:1367341/234‑1 (MQ=255)
                                                                                                                                                                                                          agCGTTTCCGTCTGCAAACCCCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTAACCCGCCCGAATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTAcg         <  1:996926/238‑1 (MQ=255)
                                                                                                                                                                                                          agCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGGGGGAGAAAGGGTTCAGTTTCGAAGGACGGTTTTTTCTGgggccccaaataagg        >  2:1294721/1‑228 (MQ=255)
                                                                                                                                                                                                          agCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGACCAGTACTGGCAGGTGCGTGAAAAAGGGTTCGATTACGAAGGTGGTTTTCGTCTGGCGCACGAATTAcgc        >  1:1018922/1‑239 (MQ=255)
                                                                                                                                                                                                          agCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCCCATGAAAGGCCAGTACTGGCAGTGACGGGGAAAAGGGTTCGATTACGAAGGGCGGTTTTGTGTGGGGCACGagtaaaac        >  2:962271/1‑232 (MQ=255)
                                                                                                                                                                                                                tCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGGTTACGAAGGTCGTTTTCGTCTTGCGCCCGAATTAAGCGGTTaa  >  1:887410/1‑239 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
CGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGATCACCGCCGCCGATATCCTCGCCCAGCAAGCCTTATTGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAA  >  NC_000913/2281947‑2282392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: