Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,321,376 T→C 7.4% L445P (CTC→CCC)  atoS → sensory histidine kinase in two‑component regulatory system with AtoC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,321,3760TC 7.4% 369.7 / 2.3 131L445P (CTC→CCC) atoSsensory histidine kinase in two‑component regulatory system with AtoC
Reads supporting (aligned to +/- strand):  ref base T (64/56);  new base C (3/8);  total (67/64)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTC  >  NC_000913/2321153‑2321612
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
 ccgCCCGCAAAAAAACCCAGCGCCGCATGGCGCAAGAAGAACGCCTCGCCACCCTGGGTGAGCTGATGGGTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCa                                                                                                                                                                                                                               <  2:1000878/237‑1 (MQ=255)
      cggaaaaaaACCCGGGGGCGCATGGCGGAAGGAGAACGGCTCGGCCCACTGGGGGAGCGGATGGCTGGCGTCGGGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCca                                                                                                                                                                                                                          <  1:1300537/236‑1 (MQ=255)
      cGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGGTATGTCCAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATACCTGTCCGTAGTACTCAACAAAAACGACTCAATTCACAAAGATATTCAGCAATTTACCGAATTTTCACGTCCAccccc                                                                                                                                                                                                                          >  2:1022457/1‑238 (MQ=255)
      cGCAAAAAACCCCGGGGCCGCATGGCCCAAGCAGAAGCCCTCGCCACACTGGGTGAGCTGATGGCTGGCTTCGCGCATAAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCca                                                                                                                                                                                                                          <  2:940537/239‑1 (MQ=255)
                   aGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACAAGTGACCCAATACATCAGGAATATCTGTCCGTAGAACTCAAAGAAATCGATTCAATTATCAACATATTTCAGCAGTTGCTCGAATTTTCACGTCACAGCCACAGCCaaggtaaa                                                                                                                                                                                                             >  1:1234923/1‑233 (MQ=255)
                     cgccgcATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCAAAGCAAATGGcaaaa                                                                                                                                                                                                           >  2:709545/1‑237 (MQ=255)
                        gcggagggcataGCAAAAGGCCCGGCCACCCTGGTTGGGCTGATGGCTGGCGTCGGGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGAACAGATCTTGCGCAAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGt                                                                                                                                                                                                        <  2:1241034/228‑1 (MQ=255)
                               cgcAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCCAATTTTCACTTCCACCCCCAAGTCAATGGcaaaaaaacaacccc                                                                                                                                                                                                 >  2:1057458/1‑230 (MQ=255)
                                      aGACCGCCTGGCCACATTGGGGGTGCTGATGGCTGGCGTGGAGTATGAAATACGTAATCAATTAACGGCTATTCGTGGTTAGGTACAGATCTTCCGGCAAAAAACCAGTGCCCCAATGCAACAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAAGAAGTGCTCGAAGTTTCACGCCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCa                                                                                                                                                                                           <  2:417630/238‑1 (MQ=255)
                                         aCGCCTCGCCACCCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCa                                                                                                                                                                                           <  1:709545/235‑1 (MQ=255)
                                         aCGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTgg                                                                                                                                                                                       <  1:1057458/239‑1 (MQ=255)
                                                gCCCCACGGGGTGGGGTTATGGTGGGGGTCGCGCAGGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGCAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                <  2:1340672/239‑1 (MQ=255)
                                                gCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                >  1:219563/1‑239 (MQ=255)
                                                cgcacaCTGGGTTAGCTGATGGGTGGCGTCGCGGATGAAGTACGTAATCCGTTAACGGCTTTTCGTGGTTATGTACAGATCTTGCGACAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                <  1:69826/237‑1 (MQ=255)
                                                cccACCCTGGGTGTGTTTATGGTTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                <  2:17964/238‑1 (MQ=255)
                                                     acTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAActc                                                                                                                                                                          <  1:1347534/239‑1 (MQ=255)
                                                           tGAGCTGATGGCTGGGGTCTGGCATGAAGTACGTAATCCCTTACCGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  1:1304494/238‑1 (MQ=255)
                                                           tGAGCTGATGGCTGGGGGCGCGCATGAAGGACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  1:1060487/238‑1 (MQ=255)
                                                           tGAGCTGATGGCTGGCGTCGCGAATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  1:526313/238‑1 (MQ=255)
                                                            gAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACGGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggt                                                                                                                                                                      >  1:766830/1‑236 (MQ=255)
                                                            cggtgggtgggtgggGGCGGGCAGGAAATACGTAATCCCTGTACGGATATGCGTGGTTATTTACAGCTCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACACAAAGAAATCGATCCAATTAACAAAGTTATTCAGCAATTCCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGATGAAGAAACTctggtac                                                                                                                                                                    <  2:745941/231‑1 (MQ=255)
                                                               cTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATCCATCAGGAATATCTGTCCGTAGCACTAAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCCCGAATTTTACCGTCCCACCCACAGGAACTGGCAAAAAGTCAGCCGCAATTCATTGGTTAAAGAAATCTctggcagagt                                                                                                                                                                 >  2:910009/1‑233 (MQ=255)
                                                               cTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAAATCTTGTGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACTTCCACGCCCCAGTCAATGGCAACAAGTCGGCCTCAATGCATTGGTTGAAGAAACCctggtactgga                                                                                                                                                                >  2:1115287/1‑238 (MQ=255)
                                                                   tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTc                                                                                                                                                                                                       >  1:708693/1‑197 (MQ=255)
                                                                   tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTCCTCGAATTTTCACGTCCACGCCACAGTCAATGGCACCACGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACaa                                                                                                                                                            >  1:62232/1‑239 (MQ=255)
                                                                        ggcGTCGCGCAGAAAGTCCGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGACCTTGCGCAAACAAACAAGTGACCCAATAAATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAAccgc                                                                                                                                                       <  1:864843/239‑1 (MQ=255)
                                                                            tCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCCCCCCACAGTCAATGGCAACAAGTCAGCCTCCATGCCATGGTTGAAAAAAATCTGGTACTGGGAAAAACCGCCGGc                                                                                                                                                   >  2:118734/1‑239 (MQ=255)
                                                                                cgaagAAGTTCGTAACCCCGTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAAAAAACCGGTGACCCAATACATCAGGAATATCTGTCCGTAGTCCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTa                                                                                                                                                <  1:1130894/234‑1 (MQ=255)
                                                                                          cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCggg                                                                                                                                      >  1:237654/1‑238 (MQ=255)
                                                                                                 cGTTAACGGCTATTCGGGGTTAGGTCCAGTTTTTGCGCCAACAAACCAGTGACCCAATACATAAGGAATACCGGTCCGTAGTACTCAAAGAAATGGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGAc                                                                                                                                 <  1:634641/236‑1 (MQ=255)
                                                                                                  gTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCa                                                                                                                             >  1:839582/1‑239 (MQ=255)
                                                                                                    tAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCCGGCACTGGACCAAACCGCCGGCGTACAAGCGCCGGGCGACTTCATa                                                                                                                           >  1:604108/1‑239 (MQ=255)
                                                                                                     aaCGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCCTTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCACGGGTCGACTTCATaa                                                                                                                          >  1:688160/1‑239 (MQ=255)
                                                                                                          cttttCGTGTTTTTGTCCAGATCTTGCCCCAACAAACCAGTGACCCAAAACAACAGGAATTTCTGTCCGTAGTTCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGaa                                                                                                                     <  2:839582/236‑1 (MQ=255)
                                                                                                           tATTTGTGGTTATGTCCAGATTTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAACCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGaa                                                                                                                     <  1:818671/238‑1 (MQ=255)
                                                                                                           tATTCGTGGTTATGTACAGATCTTGCGCAAAAAAACCAGTGACCCAATCCATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGaa                                                                                                                     <  1:118734/238‑1 (MQ=255)
                                                                                                           tATTCGGGTTTACGTAAAGATCTTGCGCCAACAAACCAGTGACCAAATCCATCAGAAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAGGTTATTCAGCAATTGCTGGAATTTTCACGACCACGCCACAGTCAATGGCACCAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGaa                                                                                                                     <  1:1022457/238‑1 (MQ=255)
                                                                                                             ttCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCt                                                                                                                                                                                                                                               >  2:1351750/1‑115 (MQ=255)
                                                                                                             ttCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCt                                                                                                                                                                                                                                               <  1:1351750/115‑1 (MQ=255)
                                                                                                             ttCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCAt                                                                                                                                                                                          >  1:394938/1‑168 (MQ=255)
                                                                                                             ttCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAACAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCAt                                                                                                                                                                                          <  2:394938/168‑1 (MQ=255)
                                                                                                                  tgTTATGAACAGTTCTTGCTCAAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTAAAAGAAATCGATTCAATTAAAAAAGTTATTCAGCAATTGCCCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATaa                                                                                                             <  1:683340/238‑1 (MQ=255)
                                                                                                                  gtttgtGTACAGTTCTTGCGCAAACAAACCAGTGACCCAATACATCAGGAATTTCTGTCCGTAGTACTAAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCCCGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATaa                                                                                                             <  2:62232/234‑1 (MQ=255)
                                                                                                                  ggTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                >  2:921342/1‑173 (MQ=255)
                                                                                                                  ggTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                <  1:921342/173‑1 (MQ=255)
                                                                                                                       tGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACCGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAAt                                                                                                        >  1:204899/1‑239 (MQ=255)
                                                                                                                            caaccTTGCCACAAAAAACCGATGACCCAATCCATCAGAAATATCTGTCCGTAGTCCTAAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGc                                                                                                   <  1:1263971/235‑1 (MQ=255)
                                                                                                                              aTCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGccc                                                                                                 >  1:79439/1‑239 (MQ=255)
                                                                                                                               ttttGCGCCAAAAACCCATCGACCAAATACATCAGGAATTTCTTTCCGTATTACTAAAAGAAACCGATTAAATTAAAAAAGTTATTCAGAAATGGCTCGAATTTTCACGTCCCCGCCACAGTCAATGGCAACAAGTCAGCCTAAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGc                                                                                                   <  2:239156/234‑1 (MQ=255)
                                                                                                                                cTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATGGGTTGAAGAAACTCTGGTACTGGTACAAACAGCCGGCGTAAAAGAGCGGGACGACTCCTAAAGCGAACTGGCAAATGAATAAAGACCGa                                                                                               >  2:914417/1‑239 (MQ=255)
                                                                                                                                cTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGACTCAATTAACAAAGTTATTCAGCAATTGCCCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAACCTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGa                                                                                               >  1:247916/1‑239 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTcc                                                                                                                                                                                                                                <  1:655801/108‑1 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTcc                                                                                                                                                                                                                                >  2:655801/1‑108 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     >  1:1350292/1‑166 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGGACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTCCAAGCGCGGGTCGACTTCAGAAGCGAACTGGATAATGAATTAAGCCCGATTa                                                                                            >  1:699835/1‑239 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATACAACAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  2:1350292/166‑1 (MQ=255)
                                                                                                                                   gcgcCAACAAACCAGTGACCCAATAAATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACAGCCGGCGTACAAGCGCGGGGCGACTTCATAAGAGAACTGGATAATGAATTaacacgcatta                                                                                            >  2:1037146/1‑230 (MQ=255)
                                                                                                                                       caacaaACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGc                                                                                        <  1:863836/239‑1 (MQ=255)
                                                                                                                                       caacaaACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGAAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGc                                                                                        <  2:237654/239‑1 (MQ=255)
                                                                                                                                                  tGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTa                                                                                                                                                <  1:956902/172‑1 (MQ=255)
                                                                                                                                                  tGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGAACAAACCGCCGGCGTa                                                                                                                                                >  2:956902/1‑172 (MQ=255)
                                                                                                                                                      ccAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  1:252568/147‑1 (MQ=255)
                                                                                                                                                      ccAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     >  2:252568/1‑147 (MQ=255)
                                                                                                                                                      caattcctttaGGAATTTTTGTCCGTTGTACCAAAAAAAATCGATTAAATAAAAAACTTTATTCAGCAATGCCTCGAATTTACCCGTCCACGCCACAGTCAATGGCAACAAGTCGGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCt                                                                         <  2:709012/229‑1 (MQ=255)
                                                                                                                                                         aaacaacaGGAATATCTGTCCGTAATACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGAAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCaa                                                                      <  1:56630/237‑1 (MQ=255)
                                                                                                                                                         aTACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCCCaa                                                                      >  1:503523/1‑239 (MQ=255)
                                                                                                                                                           aCATAAGGAATTTTAGGCCGTACTTCACAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGATTTTCCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAAc                                                                    <  1:712364/239‑1 (MQ=255)
                                                                                                                                                              taaGGATTATCTGTCCGTAGTACCAAAAGAAACCGATTTAATTAACAAAGTTATTCCGCAATTGCTCGAATTTCCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAgg                                                                 <  1:102180/237‑1 (MQ=255)
                                                                                                                                                              tCAGAAATATTTGGCGGTGTTACAAAAAGTAATCGTTCAAATTAACAAAGTTATAAGACAAGTAGCGGAATTTTACAGCTCACGACAAATTCAATGGCTACCAGTCAGCCTCAATGCAATGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAgg                                                                 <  2:835422/239‑1 (MQ=255)
                                                                                                                                                              tCAGAAATATCTGGCCTTAGTTCTCAAAAAAATCGATTAAATAAACAAAGTTATTCAGCAATGGCTCGAATTTTCAGGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAgg                                                                 <  2:79439/239‑1 (MQ=255)
                                                                                                                                                               cAGGAATATCTGTCCGTCGTACTCAAAGAAATCGTTTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCAGGGACTGGTACAAACCGCCGGCGTACAAGCACGGGTCTACTTCATAACGGAACAGGATAAAGAATAAGGCCCGATTAACGCCGACGGAAAACTGCtacaaaagtt                                                                >  2:1116328/1‑230 (MQ=255)
                                                                                                                                                               cAGGAATATCTGTCCGTAGTACTCAAAGAAATCGCTTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGCCGACCAAGCGCGGGCCGACTTCGTAAGCGAACTGGATAATGAATTAAGCCCGCTTAACGCCGATCGTGAAACGCTAAAACAgga                                                                >  2:838205/1‑238 (MQ=255)
                                                                                                                                                                aGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGTTAATGAATTAAGCCCGATTAACGCCGTTCGTGACCTGCTCAACCAgga                                                                >  1:411070/1‑237 (MQ=255)
                                                                                                                                                                     tatCTGTCCGTAGTCCCAAAAGAAATCGATTCAATAACAAAATTTATCCAGCAATGTCTCGAATTTACCCGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTactact                                                          <  1:324796/239‑1 (MQ=255)
                                                                                                                                                                       tCTTTCTGTATTACGCAAAAAAATGGTTTAAATTAACAAAGTTATTCAGAAATTGCCGGAATTTCACCGTCACCGCAACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGa                                                        <  2:1273128/239‑1 (MQ=255)
                                                                                                                                                                       tCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACCCCGATCGTGAACTGCTCAAAAAGGAAAAACTGa                                                        >  2:138742/1‑239 (MQ=255)
                                                                                                                                                                       tCTGCCCGTAGTACCCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGa                                                        <  1:138742/239‑1 (MQ=255)
                                                                                                                                                                          tttcttatgtcTCAAAATAATTGTTTCAATTAAAAAATTTATTCACCCATTCCTCAAATTTTCCCGTCCACGCCCAAGTCAATGGCAAAAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACGGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAata                                                     <  2:384187/229‑1 (MQ=255)
                                                                                                                                                                          gTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGCCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGCTTAACGCCGAACGTGAACTGCTCAAAAAGGAAATACTGaaa                                                      >  2:932153/1‑237 (MQ=255)
                                                                                                                                                                           tCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCa                                                                                                                                                                                           >  1:4716/1‑105 (MQ=255)
                                                                                                                                                                           tCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCa                                                                                                                                                                                           <  2:4716/105‑1 (MQ=255)
                                                                                                                                                                            cccttataccaaaaaaaaaCGGTTCAATTAAAAAATTTATCAAGAAATTCCTAGAATTTTCACTGCCACGCCACAGGCAATGACAACAAGGCAGCCCCAAGGCATTGGTGGAAGAAACTCTGGTACTGGGACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATc                                                   <  2:688160/228‑1 (MQ=255)
                                                                                                                                                                            ccGTATTACTCAAAGAAACCGATTAAATTAACAAAGTTATTCAGCATTTGCTCGAATTTTCACGTCCCCGCCACAGTCAATGGCAACAAGTCTGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGAATAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATc                                                   <  1:1115287/239‑1 (MQ=255)
                                                                                                                                                                            ccGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATc                                                   <  1:981644/239‑1 (MQ=255)
                                                                                                                                                                               tagtaCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                <  2:174410/112‑1 (MQ=255)
                                                                                                                                                                               tagtaCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTgaaga                                                                                                                                                                                >  1:174410/1‑112 (MQ=255)
                                                                                                                                                                                agtaCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTctggta                                                                                                                                                                     <  2:490418/121‑1 (MQ=255)
                                                                                                                                                                                agtaCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACAACGTAAAATCCTGa                                               >  2:1262362/1‑239 (MQ=255)
                                                                                                                                                                                  taCTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTactact                                                          >  1:1226561/1‑226 (MQ=255)
                                                                                                                                                                                      cAAAAAATTCGATTAAATTAACAATTTTATTCAGCAATTGCTCAAATTTTCCCGGCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATg                                         <  2:277422/239‑1 (MQ=255)
                                                                                                                                                                                           aaactgATTCAATTAACAAAGTTATTCAGCAATTGCCCGAATTTTCACGCCCACGCCAAAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTc                                    <  1:190127/234‑1 (MQ=255)
                                                                                                                                                                                              tCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTGCAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAg                                 >  1:484672/1‑239 (MQ=255)
                                                                                                                                                                                              tCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAACCAGGCACTACTGAATATCCTGATCAATGCCGTCCAg                                 >  1:428644/1‑239 (MQ=255)
                                                                                                                                                                                              tCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATc                                                   >  2:818928/1‑221 (MQ=255)
                                                                                                                                                                                              tCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATc                                                   <  1:818928/221‑1 (MQ=255)
                                                                                                                                                                                               cGATTAAATTAAAAAAGTTATTCAACAATTGCTCGAATTTCAACGTCCACGCCACAGTCAATGGCAACAGGTCAGCCTCAAAGCATTGGTTGAAGAAACTCTGGTACGGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAgg                                <  1:1116328/239‑1 (MQ=255)
                                                                                                                                                                                                       ttAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGACCTGCTCAACCAGGTACTACTGAATATCCTGATCAATCCCGTCCAGGCAATAAgc                        >  2:820192/1‑239 (MQ=255)
                                                                                                                                                                                                       ttAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGAACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGTTCGTGAACTGCTCAAACAGGTactact                                                          >  1:761700/1‑205 (MQ=255)
                                                                                                                                                                                                                          aTTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTTAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCa     >  1:725116/1‑239 (MQ=255)
                                                                                                                                                                                                                             gCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGCGGGAAAATTCGCATTc  >  1:477609/1‑239 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
ACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTCAATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTCGACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTCAAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTC  >  NC_000913/2321153‑2321612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: