Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,828,506 T→G 22.1% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,5060TG22.1% 107.0 / 2.0 52V257G (GTG→GGG) srlDsorbitol‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (9/29);  new base G (11/3);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 7.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGT  >  NC_000913/2828269‑2828739
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
cGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg                                                                                                                                                                                                                                            >  1:5188/1‑239 (MQ=255)
cGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg                                                                                                                                                                                                                                            >  1:942254/1‑239 (MQ=255)
   tGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAAGGTCACCGGCGGGCAGGGGATg                                                                                                                                                                                                                                         >  1:437505/1‑239 (MQ=255)
     cATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAGTACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGAATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATCGGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGCCAGTCGACCAATGTCACCGGCGGGCAGGGGATgat                                                                                                                                                                                                                                       >  2:242298/1‑237 (MQ=255)
     cATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGACGtg                                                                                                                                                                                                                                       >  1:910298/1‑238 (MQ=255)
             gATGCTCGGTAACCTGCTGAAATCGCCGATGCTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGTCGGGCACGGTGTGTTCTGATCaa                                                                                                                                                                                                                               >  1:475786/1‑239 (MQ=255)
                 cTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGc                                                                                                                                                                                                                           >  1:1174668/1‑239 (MQ=255)
                       ttccTGCTAAAATCGCCGATGTTCCAGGCCCTTTTGCCACCATTAGCGACCAAGCTGGGTATAAAACCGGGGCAAGTCGAGGAATAGTACATAGACAAAATGCCGCTCAAACGCGGCTGCGATTATCAAGGTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGAGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgaga                                                                                                                                                                                                                      <  2:727339/236‑1 (MQ=255)
                       aaCCGGCTGAAATCGCCGTTGTTCCAGTCACTGGTGCCACAATACGCGACCAAGCTGGGTAACAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgaga                                                                                                                                                                                                                      <  1:1071305/238‑1 (MQ=255)
                            ttctgaaaggCCCATGTTTCGGTCCCTGTTGCCCCAAAACGGCACCAAGCTGGGTATCAAACCGGATCAAGGCGAGCAGTATTACTTCGACAAAGTTCCGGTCAAACGCGGCTGCGATTATCAAGATTTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCAtt                                                                                                                                                                                                                <  2:910298/230‑1 (MQ=255)
                                       cGATTTTCCCGTTCCTGTTGCCCCAATACCCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  2:1109497/239‑1 (MQ=255)
                                       cGATGTTCCAGTCACTGTTTCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  1:968551/239‑1 (MQ=255)
                                       cGATGTTCCAGGCACTGGTGCCCAAATACGCGAACAAGCTGGGTTACAAACCGGATTAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  1:623468/239‑1 (MQ=255)
                                        gATGTTCCCGTCACTGGTGCCACAATACGCGACCAAGGTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGCAAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGt                                                                                                                                                                                                    <  1:162460/239‑1 (MQ=255)
                                          tGTTCCAGGCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGGCGAGCAGTATTACATCGACAAAGTGCCGATCAAACGAGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCCCCGGACAGTCGATCAAGGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                                                                                  <  1:154975/239‑1 (MQ=255)
                                                    aCTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGCGCTGAATATGATGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTAACCGGCGGCCAGAGGCGATTCTGGTCACAAGCGGAGATCACTTAAGGATCCCCGCGAGACTATTGaa                                                                                                                                                                                       >  1:557805/1‑239 (MQ=255)
                                                     atGTTGCCAAAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAAt                                                                                                                                                                                      <  1:452259/238‑1 (MQ=255)
                                                                aTACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGTTGTTCTGATCAACAACGGGGAACCATTAAGGATTTCCGCGGGACAATAGAATGCCTCAtcgg                                                                                                                                                                            >  1:590781/1‑235 (MQ=255)
                                                                  tcgcgACCAACCGGGGTATCAAACCGGATCAAGTCGGGCAGTATTACATCGACAAAGTACCGCTTAAACGGGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTa                                                                                                                                                                         <  2:571770/238‑1 (MQ=255)
                                                                    gcgACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGGTCAAAAACGGGGATCCATTAAGGGTTTCCCGGGGACTATAACATGCCTTATGTGGTAc                                                                                                                                                                        >  1:235275/1‑238 (MQ=255)
                                                                          aaGCTTGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTTCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcatc                                                                                                                                                                 <  1:459040/239‑1 (MQ=255)
                                                                             cTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcat                                                                                                                                                                  <  1:1172366/235‑1 (MQ=255)
                                                                              tGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTCCCGCTCAAACGGGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                               <  1:162306/237‑1 (MQ=255)
                                                                              cgggTTTCAAACCGGATCAAGGCGAGCAGTATTTCATCGACAAAGTGCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                               <  2:590781/236‑1 (MQ=255)
                                                                                ggTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcatc                                                                                                                                                                 <  1:164950/233‑1 (MQ=255)
                                                                                    tCAAACCGGAACAAGTCGGGCAGTTTTACATCGACAAAGTACCGCTCAAAAGCGGCTGCGATTATCAAGATGTGCTTAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACa                                                                                                                                                       <  1:1246614/238‑1 (MQ=255)
                                                                                       aaCCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAAGGGAGGTCCCTTAAAGGACTCCCCGGGGCTATAGAATGCCCGATGCGCCACGCTCATCAGGCATACAgg                                                                                                                                                     >  1:1104747/1‑237 (MQ=255)
                                                                                             aTCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  1:994961/185‑1 (MQ=255)
                                                                                               cAAGTCGAGCAGTTTTACATCGACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGTTGTTCTACGCCAGTCCTAAGGCGTCGTCCTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCa                                                                                                                                           <  1:1101839/239‑1 (MQ=255)
                                                                                                           tATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  1:850932/171‑1 (MQ=255)
                                                                                                            tttcacgcGAAAAAGTACGGTGAAACCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTTTTCTACCCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCTTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGa                                                                                                                               <  1:242298/231‑1 (MQ=255)
                                                                                                            aTTACTCCGAAAAAGCGCCGCACAAACGGGGCTGGATTTCACAAGAGGTGCAGAATATGGTGGTGTTCTGCCCCAGTCGTAGGGGGTGGTACTGCACGGGACAGTCGATAAATGTCACCGGGGGTCAGGTGATGTTCTGATCAACAGGGGAGATCCATTAAGGATCCCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaa                                                                                                                              <  2:1174668/239‑1 (MQ=255)
                                                                                                            aTTACACCGACAAATTTCCGGTCAAACGGGGCTGCGATTATCAAGATGGGCTGAATATGCTGCTTTTCTACGCCAGTCCTAGGGCGTCGTACTGCACCGGGCAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaa                                                                                                                              <  1:1301136/239‑1 (MQ=255)
                                                                                                                cATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                                                                                       <  1:224272/195‑1 (MQ=255)
                                                                                                                     aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGCTGTTCTGATCACCAGCGGTGATCCATTAAGGATCTCCGTGGGACTATATAATACCTGATGCGCCACGCCCATCAGACACACAAGGATTTCCCCCACCACAAAAAGAaaaaaaaggg                                                                                                                      >  2:30472/1‑233 (MQ=255)
                                                                                                                               gCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGGGGGACTATAGAATGCCAGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTCCATTAAGGAAAAGTTATGGGATCCGcca                                                                                                            >  1:852683/1‑236 (MQ=255)
                                                                                                                                cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATAAACAGCGGGGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTCCATTAAGGAAAAGGTATGGGATcccccct                                                                                                           >  1:1276161/1‑233 (MQ=255)
                                                                                                                                cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGGTCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGAATACAGGACTTCCGCCACCACAATAAGTAAAAAGTATGGAATCCGccccc                                                                                                          >  1:928777/1‑235 (MQ=255)
                                                                                                                                                            tgCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                                                                                       <  2:990966/151‑1 (MQ=255)
                                                                                                                                                            tgCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                                                                                       >  1:990966/1‑151 (MQ=255)
                                                                                                                                                                  atgtgctttttttCGACGCCAGTCCTAGGTGGTCCTTCGCCACCGGACAGGAGATAAATGTCACCGGCGGTCAGGGTATTTTCTGATCAACAGGTGAGATCCTTGAAGGATCTCCGTGAGACTAGAGAATGCCTGATGCGCTACGCTCATCAGGCATACGGGTCTTCCGCCACTACATTAAGGAAAAGTTATGGTAGCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCaa                                                                         <  2:569224/227‑1 (MQ=255)
                                                                                                                                                                   tatGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                     <  1:185729/115‑1 (MQ=255)
                                                                                                                                                                             ttCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGGTCAACAGCGGGGATCCATTAAGGAACTCCGTGAGACtata                                                                                                                                                                                          >  2:185729/1‑115 (MQ=255)
                                                                                                                                                                               ctcccgcAGTCCTAAGGGGTTGTACTGCACCGGCCAGTCGATCAATGCCCCCGGCGGTCAGGTGATTTTTTGATCAACGGCGGAGATCCATTAAGGATCTCCGTGAGACTAAAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcgg                                                           <  1:1151815/233‑1 (MQ=255)
                                                                                                                                                                                       gTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGGTCTGATCAACAGCGGAGATCCATTAAGGGCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCCCTCATCACCGCCGCCGTTATTGCCTGGTGTGGGGAACAGGCCTTTGGCGGCGGGCAAAt                                                  >  1:618806/1‑239 (MQ=255)
                                                                                                                                                                                          cTAGGGCGTCTTTCCGCCCCGGACAGTCAATCAAGTCCCCGGGCGTGCGGGGTGTTTTCTGAACAACAGCGGAGCTTCATTAAGGATCCCCGTGAGATTAAAGAATGCCTGATGCGCTACGCTAATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAAttt                                                <  2:68946/239‑1 (MQ=255)
                                                                                                                                                                                                      tctcccccGGACACTACATTATTGTCCCCGGGGGTCAGTTGTTGTCTTGATCAACAGCGGCAATCCATGAAGTATCTCGGGAGCAATAAAGAATGCCTGATGCGCTACGCTAATGAGCCATACAGTACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAAcc                                    <  2:437505/233‑1 (MQ=255)
                                                                                                                                                                                                      aCTCCACCGGAAAGGTGAATAATGTCCCCGGCGGGCAGGTTTTGTTCTGACAAACGGGTGAGATCCATAAAGGATTGCCGTGAGACTATAGAATGCCAGATGCGCTACGCTCAACAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAAcc                                    <  1:1236999/239‑1 (MQ=255)
                                                                                                                                                                                                               gACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAACTCCGGGAGACTATAGAATTCCTGATGCGCTCCGCTCATCAAGCATACAGGACTTCCGCCACTACATTAAGGGAAAGTTATGGTATCCGCACTCATCACCGTCCCCGTTTTCGCCCGCGGTGGTGAACTGGCCTTCGGCGGCTGGCACCTTTTTCCTTTTTACCGCGCCTTc                            >  2:750030/1‑238 (MQ=255)
                                                                                                                                                                                                                    tCGATCAATGTCACCGGCGGTCAGGTGGTGTTCTGCTCAAAAGCGGCGAACCATTAAGGGTCTCCGTGAGACTATAGAATGCCCGATGCGCTACGCTCATCAGGCATAAAGGACTTTCGCCACTACATTTAGGAAAAGATTTGGGATCCGCACTCATCACCCTCCCCCTTTTCGCCTGGGGGTCGGAAATGGCCTTAGGCGGCTGGGAAAATTTTCGCTTTTACCGTGCTTTCgcaaca                      >  2:1292416/1‑234 (MQ=255)
                                                                                                                                                                                                                     cGATCAATGTCACCGGCGGTCAGGGGATGTCCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGaca                        <  1:1316848/236‑1 (MQ=255)
                                                                                                                                                                                                                                          cAGGTGATGTTCGGATCAACAGCGGAAATCCATTAAGGCTCCCCGTGGACCTAAAGAATGCCGGATGCGCTACGCTCATCAGGAATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGt  <  1:1281804/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGT  >  NC_000913/2828269‑2828739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: