Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,560,455:1 | +G | 100% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,560,455 | 1 | . | G | 95.4% | 193.0 / ‑0.9 | 65 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (47/15); total (50/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACC > NC_000913/3560218‑3560672 | aTCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCCTGTT‑G‑Gc > 1:408222/1‑238 (MQ=255) ttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAg > 1:230400/1‑239 (MQ=255) cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCGGGATCAGGTTTTg > 1:371805/1‑238 (MQ=255) tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGATCTGGGTGGCCTTGTGGTCGGG‑CCACGCGGTGTTAACCGGACTGGAAGCGCGCG‑CCG‑CCCCGCCATGCTGG‑GC‑CACGGCCAGGTTTTGCTcc > 2:207805/1‑239 (MQ=255) ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGGG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTc > 1:146412/1‑239 (MQ=255) ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACGGCCATGAT‑G‑GCGCAGGACACGGGTTTGCTCCCCCAGccc > 1:25515/1‑237 (MQ=255) ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGcacgct > 1:926063/1‑233 (MQ=255) tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGCGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAGGTTTTGCTCCGCCAGCCCAtta > 1:23975/1‑238 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCCGCG‑CCG‑CCCCGCCATGAG‑G‑GCGCAGGGCCAGGTTTTGCCCCGCCAGCCCATTGGGGGcg > 2:639033/1‑239 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTcg > 1:193681/1‑239 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAGGTTTTGCTCCCCCAGCCCATTGGGGGcg > 2:601828/1‑239 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCC‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc > 1:172146/1‑238 (MQ=255) cTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGGGGGGAAAAg > 2:378709/1‑238 (MQ=255) gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGGGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg > 1:840054/1‑236 (MQ=255) gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGGGGTCGCGGCGAAAAGTCTGCGGGCTg > 1:363446/1‑239 (MQ=255) tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑GCCC‑CGGGGTCAGGTTTTGCTCCGCCAGCCCATTGGGGGCCCGGCGAATAGTGTGGGGGCTGACgg > 2:588318/1‑239 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCCTGATGG‑GG‑CAGGGCCAGGTTTTGCCCCGCCAGGCCATTGGGGTCGCGGCGAAACGTCTGGGGGcgtggggg > 2:119791/1‑232 (MQ=255) cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGCAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCACCCCATTGAGGTCCCGGCGAATAGTCTCCGGGCTGACGgag > 1:405478/1‑239 (MQ=255) cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag > 1:1032872/1‑239 (MQ=255) cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCACCG‑CCG‑CCCCGCCATGAT‑G‑CCGCGGGATAAGGTTTTGCTCCGCCACCGCATTGAGGCCGCGGCGAATAGCCTGGGGGCTGGCGgag > 2:712952/1‑239 (MQ=255) gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg > 1:432817/1‑233 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCGGCCCATTAAGGTCGCGGCGAAAAGTCTGCGGGCTGCCGGAGCAATGc > 1:593407/1‑237 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGACGGGGAAATGc > 1:1052287/1‑237 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGGGAAAAGTCTGCGGGCGGCCGGGGAAAGGcc > 2:1058587/1‑237 (MQ=255) cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCCCCAGCCCATTGAGGTCCCGGGGAAAAGTTTGGGGGGTGAGGGGGAAAGTCTCCAcc > 2:765823/1‑239 (MQ=255) ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTACTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGCGGAATGCTATACCa > 1:160715/1‑237 (MQ=255) ggggggcgtatatCAAAAAACGGGGTCGAGCCTTTGGGGTTTTG‑CCCCCCCCCTTGGCGGGCAATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑CCACGGGGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt < 1:639033/229‑1 (MQ=255) gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAATGCAGCG‑CCG‑CCCCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAAAATTCTCCGGGCTGCCGGGGAAATACTCTACAACCACTTCggg > 2:457801/1‑238 (MQ=255) gCCAATATGGAAAAACGGCGTCGGGCCTTTGGGGATTTC‑CTCCGCCACTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 1:765823/238‑1 (MQ=255) atatCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAACTGCTCTACCAGCCCTTCGGTCCTgcc > 1:802716/1‑237 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATa > 1:1007436/1‑238 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTGGGTCGCGGCGAATAGTCTGCGGGCTGACGGGAGAATGCTCTACCAGCTCTTCGGAACTAACATaa > 1:691442/1‑238 (MQ=255) gATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGCCATaa > 1:706372/1‑238 (MQ=255) aaCCGCGTCGTGCTTTTGGGGGTTTGTCCCCGCCTCTTGGGGGGGGTGGCGCTCTTTTTCTGCGGGGTGGGGGGCCTTGCGATCGTG‑CCACGGCGTGTAAACCGAACTGGAAGGCAGCG‑CCG‑CCCCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 2:672874/239‑1 (MQ=255) cGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCGCTACCACCTCTTCGGTCCTGACCTAACCCTGCTGtt > 1:1065782/1‑239 (MQ=255) tCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa > 1:181856/1‑218 (MQ=255) tCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTCCGGGCTGCGGGAGAAATGCCTTACCCGCTCTTCGGCACTGACATAACCCTGCTGTTTa > 2:251541/1‑239 (MQ=255) aGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTCCTGTTTAAcc > 1:699526/1‑239 (MQ=255) ctttGGGGTTTGT‑CCCCGCCCGTTGGGGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGGGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg < 1:601828/236‑1 (MQ=255) tttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGCCATAACCCTGCTGGTTAAACAGTTCGaaaaa > 2:1003729/1‑237 (MQ=255) tttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAAGCCTCTACCGGCCCTTCGGAACTGACATAACCCTGCAGTTTAACCAGTTCGttaat > 1:1067179/1‑238 (MQ=255) ttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAAACCCGCGGTTTAACCAGGTCGaaaata > 2:7204/1‑238 (MQ=255) ttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTTACAAAACCCTGCTGTTTAACCTGtctcttataaa > 2:927307/1‑229 (MQ=255) ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCCGTAATACCGTtctg > 1:693774/1‑238 (MQ=255) gtttGGGGGGGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGGG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt < 1:215822/238‑1 (MQ=255) cttggggggCGATGCGCTTTTTTTCTGCGGTCTGGTGGGCCTGGCGATCGTC‑ACACGGCGTGTTAACCGAAGTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt < 1:470762/233‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCAAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGATAGTCTGCGGGCTGACGGAGGAATGCTCTACCCCCTCTTCGGTACTGCCCTAACCCTGCTGTTTAACCAGTTCGATAAAACCGTTGTGACGtttg > 2:877474/1‑237 (MQ=255) gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCCGCTGTTTAACCAGGTCGTAAAACCCGTTGGGACGTTGgggttgtt > 2:788624/1‑239 (MQ=255) ccctttttttCTGGGGTCGGGGGGGCTTTGCG‑GCGTGCCCAGGGCG‑GTGAACCCGACTGGGAGGGCGCGCCCG‑CACCGCCATGAT‑G‑GCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt < 2:405478/234‑1 (MQ=255) ttttcgcgggTCGGGGTGGCCTGGCGATCGTG‑CCGCGGCTTGTTAACCGACCGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa < 1:119791/231‑1 (MQ=255) tgcGGTTTGGGTGGCTTTGGGCTCGTG‑CCAGGGCGTGTTAACCGAGCTGGAAGGCGGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc < 2:322936/237‑1 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGGT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAAAACCGTTGTGACGTTGTGTTTTTTTCATTTTTAAATCCCTg > 2:413580/1‑239 (MQ=255) gTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTTTACCAGCCCTCCGGAACTGACATAACCCTGCTGTTTAACCAGGTCGATAAAACCGTTGGGACGTGTGGTTTTTTTCATTTTAAAATCCCCGGa > 2:353030/1‑239 (MQ=255) cggggTGGCCTTGGAATGGTG‑CCAGGGGGTGTTACCCGAACGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa < 1:788624/236‑1 (MQ=255) ccTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTAttttcg > 1:701772/1‑239 (MQ=255) cGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc > 1:396645/1‑239 (MQ=255) cGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAAAAAACCGTTGTGACGTTGGGGTTGTTTAATTTTTAAATCCCCGGAATAATTTTCGTTTTCgcgc > 2:875367/1‑239 (MQ=255) gTG‑CCACGGGGTGTTACCCGAACGGGAAGGCAGGG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa < 1:378709/239‑1 (MQ=255) gTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa > 1:545583/1‑239 (MQ=255) ggtTAACCGAACGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 1:7204/237‑1 (MQ=255) gtTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTGGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGGCCATTTAGCGAATca > 2:682661/1‑239 (MQ=255) gAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa > 1:838455/1‑238 (MQ=255) atgggAAGGCAGGC‑CCG‑CACCGCAAGTAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:877474/236‑1 (MQ=255) ttgggggCAGCC‑CCGCCCCCGCTCTGAT‑G‑GCGAAGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGGTGGGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 2:160715/233‑1 (MQ=255) aGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa > 1:84295/1‑238 (MQ=255) ccg‑ccc‑cCCCGCCATGGT‑G‑GCGCAGGAACAGGTTTGGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc < 2:1052287/238‑1 (MQ=255) | ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACC > NC_000913/3560218‑3560672 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |